Table Of ContentContents
ISSUE Nos
ISSUE No. 1, 15 JANUARY 1999
A Positive Selection for Plasmid Loss in Saccharomyces cerevisiae Using Galactose-Inducible Growth
Inhibitory Sequences
M. Kawahata, S. Amari, Y. Nishizawa and R. Akada
Cloning, Sequence Analysis and Overexpression of a Saccharomyces cerevisiae Endopolygalacturonase-
encoding Gene (PGL7)
S. Gognies, A. Gainvors, M. Aigle and A. Belarbi
Saccharomyces cerevisiae |RR1 Protein is Indirectly Involved in Colony Formation
A. Kurlandzka, J. Rytka, B. Rézalska and M. Wysocka
identification and Characterization of The Genes for Two Topoisomerase I-Interacting Proteins from
Saccharomyces cerevisiae
H. Park and R. Sternglanz
identification and Characterization of Lysine**° of Yeast Deoxyhypusine Synthase as the Site of Enzyme
Intermediate Formation
E. C. Wolff and M. H. Park
YEAST FUNCTIONAL ANALYSIS REPORTS
Disruption and Basic Functional Analysis of Five Chromosome X Novel ORFs of Saccharomyces cerevisiae
Reveals YJL125c as an Fssential Gene for Vegetative Growth
Jaquet and J.-( Jauniaux
Disruption and Basic Phenotypic Analysis of Six Novel Genes from the Left Arm of Chromosome XIV of
a sene-siggr mee cerevisiae
E. Duenas, J. Revuelta, F. del Rey andC . R. Vazquez de
Disruption of Six Novel Genes from the Left Arm of Chromosome XV of Saccharomyces cerevisiae and
Basic Phenotypic pepe of the Generated Mutants
K. Séron. M.-O. Bk el and R. Haquena r-Tsapis
BOOK REVIEW
Operating Instructions for the Yeast Machine
CALENDAR
CURRENT AWARENESS ON YEAST
EF No. 2 3 ANUARY 1999
Cloning and Characterization of 1,3-$-Glucanase-Encoding Genes from Non-Conventional Yeasts
Esteban, C. R. Vazquez de Aldana and F. del Re
Asymmetric Distribution of Phosatidylethanolamine in C. a/bicans: Possible Mediation by CDAT, a
Multidrug Transporter 4“ to ATP Binding Cassette (ABC) Superfamily
D 1, S. Krishnamurthy [ B. lL xit, C. M. Gupta, D. Sanglard and R. Prasad
Secretion of a Protoxin Post entititiiantie Controlled by NaCl in a Halotolerant Yeast, Pichia farinosa
Cir
A PDR5-\independent Pathway of Multi-Drug Resistance Regulated by the S/N4 Gene Product
Fleckensteir Shall nd G
YEAST SEQUENCING REPORTS
Over-Expression of Candida albicans Mitochondrial Ribosomal Protein S9 (MrpS9p) Disturbs Mitochon-
drial Function in Saccharomyces cerevisiae
vvN ilts ¢ > B 17K a iA P
YEAST FUNCTIONAL ANALYSIS REPORTS
Disruption of Six Novel Saccharomyces cerevisiae Genes Reveals that YGL7129c is Necessary for Growth
in Non-Fermentable Carbon Sources, YGL128c for Growth at Low or High Temperatures and YGL725w is
implicated in the Biosynthesis of Methionine
B.T A. Rodrig Torre d M. E. Cerdar 145
Disruption of Six Unknown Open Reading Frames from Saccharomyces cerevisiae Reveals Two Genes
Involved in Vacuolar Morphogenesis and One Gene Required for Sporulation
A } Santos, C. R. Vazquez de Aidana and J. L. R 15§
Disruption and Basic Phenotypic Analysis of Eighteen Novel Genes from the Yeast Saccharomyces
cerevisiae
f A y A Kerct . 1 Exaerd nd FF ry 165
CURRENT AWARENESS ON YEAST 173
Characterization of the Hansenula polymorpha CPY Gene Encoding Carboxypeptidase Y
R Ff K K B. Rechinaer. M. Yav M ant - A KW. Kie 121
Dynamic Responses of Reserve Carbchydrate Metabolism Under Carbon and Nitrogen Limitations in
Saccharomyces cerevisiae
D., f " " , c Ba B sonza { i 101
Spontaneous Mutation, Oxidative DNA Damage, and the Roles of Base and Nucleotide Excision Repair in
the Yeast Saccharomyces cerevisiae
YEAST FUNCTIONAL ANALYSIS REPORTS
Systematic Identification, Classification and Characterization of the Open Reading Frames which Encode
Novel Helicase-related Proteins in Saccharomyces cerevisiae by Gene Disruption and Northern Analysis
’ } \A \ EF hia ka Y. Muraka iT Ek 19
YEAST SEQUENCING REPORTS
Analysis of TFIIH Subunit, Through Isolation of the Gene from Schizosaccharomyces pombe Correspond
ing to that of Saccharomyces cerevisiae SSL1, Reveals the Presence of Conserved Structural Motifs
CURRENT AWARENESS ON YEAST 2¢
A 2-um DNA-Based Marker Recycling System for Multiple Gene Disruption in the Yeast Saccharomyces
cerevisiae
Yeast Vitality During Cider Fermentation: Assessment by Energy Metabolism
identification and Characterization of Individual Cyclin-Dependent Kinase Complexes from Saccharo
myces cerevisiae
Cloning, Targeted Disruption and Heterologous Expression of the K/uyveromyces marxianus Endopoly
galacturonase Gene (EPG7)
YEAST SEQUENCING REPORTS
Isolation and Sequence of the GCR3 Homologue from Candida albicans by Complementation of Agcr3
Strain of Saccharomyces cerevisiae
YEAST FUNCTIONAL ANALYSIS REPORTS
Transcript Analysis of 250 Novel Yeast Genes from Chromosome XIV
Basic Phenotypic Analysis of Six Novel Yeast Genes Reveals Two Essential Genes and One which Affects
the Growth Rate
ISSUE No. 5, 30 MARCH 1999
Regulation of the Expression of the Kluyveromyces lactis PDC? Gene: Carbon Source-Responsive
Elements and Autoregulation
M. Destruelle, R. Menghini, L. Frontali and M. M. Bianchi 361
A Single Nucleotide Change in the MNA7 (VCX7/HUM7) Gene Determines Resistance to Manganese in
Saccharomyces cerevisiae
L. del Pozo, L. Osaba, J. Corchero and A. Jimenez
Characterization of the Histidine Mutants of K/uyveromyces /actis
B. J. M. Zonneveld, L. Tizzani and M. Wésolowski-Louvel
Biosynthesis of Phytochelatins in the Fission Yeast. Phytochelatin Synthesis: A Second Role for the
Glutathione Synthetase Gene of Schizosaccharomyces pombe
A. Al-Lahham, V. Rohde, P. Heim, R. Leuchter, J. Veeck, C. Wunderlich, K. Wolf and M. Zimmermann
Initial Characterization of the Nascent Polypeptide-Associated Complex in Yeast
B. Reimann, J. Bradsher, J. Franke, E. Hartmann, M. Wiedmann, S. Prehn and B. Wiedmann
YEAST SEQUENCING REPORTS
Isolation and Characterization of the AF72 Gene Encoding Alcohol Acetyitransferase II in the Bottom
Fermenting Yeast Saccharomyces pastorianus
H. Yoshimoto, D. Fujiwara, T. Momma, K. Tanaka, H. Sone, N. Nagasawa and Y. Tamai 409
DNA Sequencing and Analysis of a 40 kb Region from the Right Arm of Chromosome II from Schizo-
saccharomyces pombe
M. Sanchez, F. del Rey, A. Dominguez, S. Moreno and J. L. Revuelta 419
YEAST FUNCTIONAL ANALYSIS REPORTS
The Open Reading Frame YALO48c Affects the Secretion of Proteinase A in S. cerevisiae
A. M. Wolff, J. G. L. Petersen, T. Nilsson-Tillgren and N. Din
Disruption and Phenotypic Analysis of Seven ORFs from the Left Arm of Chromosome XV of Saccharo-
myces cerevisiae
K. Hajji, J. Clotet and J. Arino 435
ISSUE No. 6 APRIL 1999
Functional Analysis of HO Gene in Delayed Homothallism in Saccharomyces cerevisiae wy2
K. Ekino, |. Kwon, M. Goto, S. Yoshino and K. Furukawa
Two-Dimensional Analysis of Proteins Secreted by Saccharomyces cerevisiae Regenerating Protoplasts
A Novel Approach to Study the Cell Wall
M. Pardo, L. Monteoliva, J. Pla, M. Sanchez, C. Gil and C. Nombela 459
Fzf1p of Saccharomyces cerevisiae is a Positive Regulator of SSU7 Transcription and its First Zinc Finger
Region is Required for DNA Binding
D. Avram, M. Leid and A. T. Bakalinsky
Analysis of Genetic Interactions Between DHH1, SSD17 and ELM17 Indicates their Involvement in Cellular
Morphology Determination in Saccharomyces cerevisiae
H. Moriya and K. Ilsono 481
YEAST SEQUENCING REPORTS
Schizosaccharomyces pombe stt3* is a Functional Homologue of Saccharomyces cerevisiae STT3 which
Regulates Oligosaccharyltransferase Activity
S. Yoshida, A. Matsuura, J. Merregaert and Y. Anraku
YEAST FUNCTIONAL ANALYSIS REPORTS
Heterologous URA3MX Cassettes for Gene Replacement in Saccharomyces cerevisiae
A. L. Goldstein, X. Pan and J. H. McCusker
How to Bring Orphan Genes into Functional Families
M.M. Bianchi, G. Sartori, M. Vandenbol, A. Kaniak, D. Uccelletti, C. Mazzoni, J.-P. di Rago, G. Carignani, P. P. Slonimski
ind L. Frontal 513
A Rapid Technique for the Determination of Unknown Plasmid Library Insert DNA Sequence Directly from
Intact Yeast Cells
E. R. Klebanow and P. A. Wei
CURRENT AWARENESS ON YEAST
Vectors for Rapid Selection of Integrants with Different Plasmid Copy Numbers in the Yeast Hansenula
polymorpha DL1
AA "7 >
Effects of Weak Acids on Cation Accumulation, ApH and A in Yeast
Disruption of the KEX1 Gene in Pichia pastoris Allows Expression of Full-Length Murine and Human
Endostatin
; f kk 562
Regulated Expression of the Saccharomyces cerevisiae Frelp/Fre2p Fe/Cu Reductase Related Genes
YEAST SEQUENCING REPORTS
identificatior f a Gene Encoding the Pyruvate Decarboxylase Gene Regulator CaPdc2p from Candida
The K/IPMRTI Gene of Kluyveromyces /actis Encodes for a P-type Ca ATPase
9
YEAST FUNCTIONAL ANALYSIS REPORTS
Systematic Analy f Yeast Strains with Possible Defects in Lipid Metabolism
2
senerat f Null Alleles for the Functional Analysis of Six Genes from the Right Arm of Saccharomyces
f ne | ‘ ve Il
HF
RRENT AWARENESS ON YEAST pe
F t Re etwee Cod Usage Bias and Translation Initiation AUG Context in
. ny -
‘ , ‘ , SS is by the Fission Yeast Schizosaccharomyces pombe
f ter dal Yeast Strains by Expressing the Pedi »ccus acidilactici Pediocin Gene
P| ere ; Le
‘ 7 tase f the Ma e V< t Debaryomyces hanseni .
r
N , € Schi: . haromyces pombe Required for Eff ent Cell Separaatndi Soexnua l f
T
Y
A " ‘ ; MV ) Mie t me Hybridization C
C
\ r j JALYSIS REPORTS
Cc
NA } ‘ H is ten
f Ne ff ts Re t , F Ele ste Leve f the Re I itional Repair Protein Rad54p
} 7
Manse } y ha PDD! Gene Product, Essential for the Selective Degradation of Peroxisomes Int
Hon ‘ Pa ms vce ere\ 2e Vps34) Fu
Characteristics of the Heterologously Expressed Human Lanosterol 14a-Demethylase (Other Names;
P45014DM, CYP51, P45051) and Inhibition of the Purified Human and Candida albicans CYP51 with Azole
Antifungal Agents
D. C. Lamb, D. E. Kelly, M. R. Waterman, M. Stromstedt, D. Rozman and S. L. Kelly
Combining Mutations in the Incoming and Outgoing Pheromone Signal Pathways Causes a Synergistic
Mating Defect in Saccharomyces cerevisiae
L. Giot, C. DeMattei and J. B. Konopka 765
Linear DNA Plasmid pPK2 of Pichia kluyveri: Distinction Between Cytoplasmic and Mitochondrial Linear
Piasmids in Yeasts
J. Blaisonneau, J. Nosek and H. Fukuhara
YEAST SEQUENCING REPORTS
Cloning of a Centromere Binding Factor 3d (CBF3D) Gene From Candida glabrata
T. Stoyan, R. Eck, J. Lechner, P. Hemmerich, W. Kunkel and S. Diekman 793
Isolation of a Histoplasma capsulatum cDNA that Complements a Mitochondrial NAD(+)-lsocitrate
Dehydrogenase Subunit I-deficient Mutant of Saccharomyces cerevisiae
C. H. Johnson and J. E. McEwen 799
CURRENT AWARENESS ON YEAST 805
SSUE No. 10A JULY 1999
Role of NaOQH-Extractable Cell Wall Proteins Ccw5p, Ccw6p, Ccw7p and Ccw8p (Members of the Pir
Protein Family) in Stability of the Saccharomyces cerevisiae Cell Wall
Mréa and W. Tanner o21t2]
The Topoisomerase | Poison Camptothecin Generates a Chk1-dependent DNA Damage Checkpoint Signal
in Fission Yeast
S. Wan, H. Capasso and N. C. Walworth O2<9 4
A Proposal for Nomenclature of Aldehyde Dehydrogenases in Saccharomyces cerevisiae and Characteriz-
ation of the Stress-inducible ALD2 and ALD3 Genes
P. Navarro-Avino, R. Prasad J. Miralles, R. M. Benito and R. Serranc 829
Overproduction of the Opil Repressor Inhibits Transcriptional Activation of Structural Genes Required for
Phospholipid Biosynthesis in the Yeast Saccharomyces cerevisiae
C. Wagner, M. Blank, B. Strohmanannd H.-J. Schuller
Deletion of the Carbonic Anhydrase-like Gene NCE103 of the Yeast Saccharomyces cerevisiae Causes an
Guygen sensitive Growth pares
( A. Gnann and F. K. Zir i
A pean womencteture for New Translation Initiation Factor Genes from S. pombe and other Fungi
der. H.-P. Vornloche ) W. B. Hersanhd eJ. yE. G. McCarthy
The Three Copies of the A7P7 Gene are Arranged in Tandem on Chromosome Il of Saccharomyces
cerevisiae $288C
ikeda. H. Satoh. K. Of} st T. Satoh and T. Mabuchi
Three » Genes Whose Expression is Induced by Stress in Saccharomyces cerevisiae
Arroyo and A.A. C yVvarruDias
YEAST SEQUENCING REPORTS
DNA Sequencing and Analysis of a 67-4 kb Region from the Right Arm of Schizosaccharomyces pombe
Chromosome I! Reveals 28 Open Reading Frames Including the Genes his5, pol, ppa2, rip?, rpb& and skb?
Xiang, M. H. Lyne, V. Wood, M.-A. Rajandream, B. G. Barrell and S. J. Av 893
CURRENT AWARENESS ON YEAST
SUE Ne 10B JULY 1999
SPECIAL ISSUE
Yeast Functional Analysis Reports
Supported it Dy the Yeast Industry Platforn
introduction—Yeast Industry Platform
Func tional Analysis of 12 ORFs from Saccharomyces cerevisiae Chromosome Il
yuez-Navarr Estandr J.u E. cPerehz-Or tir
Disruption of Six Open Reading Frames on Chromosome X of Saccharomyces cerevisiae Reveals a Cluster
of Four Essential Genes
Disruption and Basic Functional Analysis of Six Novel ORFs of Chromosome XV from Saccharomyces
cerevisiae
I35
Disruption of Six Saccharomyces cerevisiae Novel Genes and Phenotypic Analysis of the Deletants
: } 5 V ‘ ez P_G B Hiesta and M Remacha 345
Construction and Genetic Analysis of S. cerevisiae Deletants of Six Novel ORFs from Chromosome Il
+ M 1A \ Q
Epitope Tagging of Yeast Genes Using a PCR-based Strategy: More Tags and Improved Practical Routines
MK K ~ ) W. Zacharias B. Wins K. Nasmyth and & >chniebe 63
Chemotyping of Yeast Mutants Using Robotics
. r M. Ef 4 i \ Bradshaw P_P >10T TSK ] Maundre j
Disruption of Six Novel Yeast Genes Located on Chromosome II Reveals One Gene Essential for Vegetative
Growth and Two Required for Sporulation and Conferring Hypersensitivity to Various Chemicals
" yk AAV iigdalski, P. P. Stonimsk
A Large-Scale Sonication Assay for Cell Wall Mutant Analysis in Yeast
A. Fast Method to Diagnose Chromosome and Plasmid Loss in Saccharomyces cerevisiae Strains
K ed
Disruption of the Cytochrome c Gene in Xylose-utilizing Yeast Pichia stipitis Leads to Higher Ethanol
Production
Total Number of Coding Open Reading Frames in the Yeast Genome
Analysis of the Peroxisomal Acy!-CoA Oxidase Gene Product from Pichia pastoris and Determination of its
Targeting Signal
Transcription of the HXT4 Gene is Regulated by Gcr1p and Gcr2p in the Yeast S. cerevisiae
Hansenula Polymorpha Pex1p and Pex6p are Peroxisome-associated AAA Proteins that Functionally and
Physically Interact
Distributior f the Actin Cytoskeleton During the Cell (¢ le f Yarrowia lipolytica and the Visualization
f the Tubulin Cytoskeleton by Immunofluorescence
Hiagh-yield Secret f f Recombinant Gelatins by Pichia pastor
Jeletior f SF/7T, a Novel Suppressor of Partial Ras-cAMP Pathway Deficiency in the Yeast Saccharo
ce erevisiae, Causes G. Arrest
t in the Sa haromyces cerevisiae Gene SAC 2use Multiple Drug-Sensitivity
Schizosaccharomyces pombe Homologue of Glutathione Peroxidase vi ' foes not Contain Seleno
ced by Several Stresseasn d Works as an Antioxidant
Mu
YEAST SEQUENCING REPORTS Kin
Molecular Clonir J f Homologs of RAS and RHOT Genes from Crypté« occus neoformans
ade
YEAST FUNCTIONAL ANALYSIS REPORTS
Mor
Designer Deletion and Prototrophic Strains Derived from Saccharomyces cerevisiae Strain W303-1la
Cyst
CURRENT AWARENESS ON YEAST
SSUE No. 12, 15 SEPTEMBER 1999
Saccharomyces cerevisiae Colony Growth and Ageing: Biphasic Growth Accompanied by Changes in
Gene Expression
J.-R. Meunier and M. Choder
A Mutant for the Yeast scERV? Gene Displays a New Defect in Mitochondrial Morphology and
Distribution
D. Becher, J. Kricke, G. Stein and T. L wsky
Molecular Mechanism of the Multiple Regulation of the Saccharomyces cerevisiae ATF1 Gene Encoding
Alcohol Acetyltransferase
D Fujiv ara, O Kobayashi, H. Yoshimoto S. Harashima and Y. Tam
Con Cycle Regulation of a DNA Ligase- epee Gene (CalIG4) from Candida albicans
Andaluz, A. Ciudad, J. R. Coque, R. Calderone and G. Larriba
Endocytosis and Vacuolar Morphology in Saccharomyces cerevisiae Are Altered in Response to Ethanol
Stress or Heat =e
Meaden. N ebora, L Guldfeldt, H. Siequmfeldt and M. Jakobser
Alcohol Oxidese Hybrid retain Formed /n Vivo and /n Vitro
Nakagawa, H. Mukaiyama, H. Yurimoto, Y. Sakai and N. Kat
Gene Senkation in Schizosaccharomyces pombe Using a Temperature-sensitive Ura4p
K. Davis, C. Pateman and J. Davey
YEAST SEQUENCING REPORTS
Isolation and Expression of the Gene Encoding Mitochondrial ADP/ATP Carrier (AAC) from the
Pathogenic Yeast Candide parapsilosis
Vi. Nebohaéova, M Mar J. Nosek and J. Kolar
identification of Putative Response Regulator Two-component Phosphorelay Gene (CaSSK7) from
Candida albicans
A. Ldlelda a nadR A Calder
YEAST FUNCTIONAL ANALYSIS REPORTS
Disruption and Functional Analysis of Seven ORFs on Chromosome IV: YDLO57w, YDLO12c, YDLO10w,
VOUCSCe. YOLOOSw (APCT11), YDLOOSc (MED2) and YDLOO3w (MCD7)
KN L. iwanejko, S. Los F. Fabarnd eA. N
A Strong Carbon Source Effect is Mediated by puC Plasmid Sequences in a Series of Classical Yeast
Vectors Designed for Promoter Characterization
A. Abadiieva. PG . Hilven and yM.y Crabee
Deletion of Six shinies Reading Frames from the Left Arm of Chromosome IV of Saccharomyces cerevisiae
T r, B. Prein, A drositzG,. Daun 1S.D.K
Disruption of Six Novel ORFs on the Left Arm of Chromosome XIil Reveals One Gene Essential for
Vegetative Growth of Saccharomyces cerevisiae
x A var [ sardner. R._ N Walmsle
ERRATUM
Construction of a Lactose-assimilating Strain of Baker's Yeast
R
A Selection System for Human Apoptosis Inhibitors Using Yeast
One-step, PCR-mediated, Gene Disruption in the Yeast Hansenula polymorpha
Perdon p > 7 ) a ¥. f©Rc r it a} nd } AA\ Sivve r
Mutations that Cause Threonine Sensitivity Identify Catalytic and Regulatory Regions of the Aspartate
Kinase of Saccharomyces cerevisiae
ade9 is an Allele of SER? and Plays an Indirect Role in Purine Biosynthesis
RDp f<
Mouse CAF1, a Mouse Homologue of the Yeast POP2 Gene Complements the Yeast pop2 Null Mutation
M. , Sasar A | Y hioka, AA . AMAaatts ur » and AA . Saka
Cysteine Besynthenis 5i n Saccharomyc es cerevisiae: a New Outlook on Pathway and Regulation
S ] Kawa ‘2 la Brzvweczy and A Paszewsk 1365
Signalling between Mitochondria and the Nucleus Regulates the Expression of a New po-Lactate Dehy
drogenase Activity in Yeast
nberg and R. A. Butow
YEAST SEQUENCING REPORTS
The URA5S Gene Encoding Orotate-phosphoribosyl Transferase of the Yeast K/uyveromyces /actis
Cloning, Sequencing and Use as a Selectable Marker
Molecular Cloning and Sequence Analysis of Zygosaccharomyces rouxii ADE2 Gene Encoding a
Phosphoribosy!-aminoimidazole Carboxylase
Cloning and Characterization of the K/uyveromyces lactis Homocysteine Synthase Gene
YEAST FUNCTIONAL ANALYSIS REPORTS
Disruption of Six ORFs on Saccharomyces cerevisiae Chromosome X: the YJL069c Gene of Unknown
Function is Essential to Cell Viability
High-efficiency Gene Targeting in Schizosaccharomyces pombe Using a Modular, PCR-based Approach
with Long Tracts of Fianking Homology
CURRENT AWARENESS ON YEAST
The Proteolyt Systen f the Yeast K/uyveromyces /actis
Role of the Sulphate Assimilation Pathway in Utilization of Glutathione as a Sulphur Source by
Sa haromyces cerevisiae
The Yeast PRS3 Gene is Required for Ceili Integrity, Cell Cycle Arrest upon Nutrient Deprivation, lon
Homeostasis and the Proper Organization of the Actin Cytoskeleton
STRE- and cAMP-independent Transcriptional Induction of Saccharomyces cerevisiae GSY2 Encoding
Glycogen Synthase Dur g Diauxic Growth on Glucose
A Novel Vacuolar Protein Encoded by SSU27/MCD4 is Involved in Cell Wall Integrity in Yeast
5-—
Allelisn »f Saccharomyces cerevisiae Genes PSO6. Involved in Survival After 3-CPs+UVA Induced
Damage. and ERG Er »ding the Enzyme Stero! C-5 Desaturase ™T*r
Replicative Ageing in the Fission Yeast Schizosaccharomyces pombe
ww
YEAST SEQUENCING REPORTS
Sequence Analy f SLA2 of the Dimorphic Yeasts Candida albicans and Yarrowia lipolytica
YEAST FUNCTIONAL ANALYSIS REPORTS
Disruption and Functional Analysis of Six ORFs on Chromosome XV YOL117w. YOL115w TRF4)
YOL114c, YOL112w (MS84), YOL111c and YOLO72w
Three New Dominant Drug Resistance Cassettes for Gene Disruption in Saccharomyces cerevisiae
Lipid Composition of Subcellular Membranes of an FY1679-derived Haploid Yeast Wild-type Strain Grown
yn Different Carbon Sources
CURRENT AWARENESS ON YEAST
ISSUE No. 15 NOVEMBER 1999
Molecular Cloning and Characterization of the Gene HXK71 Encoding the Hexokinase from Yarrowia
lipolytica
T. Petit and C. Gancedo
An Alternative Model for the Transmembrane Segments of the Yeast H* -ATPase
J. P. Pardo, F. Martinez, G. Guerra, |. Velazquez, J. L. Rendén and G. Mendoza
FLR1 Gene (ORF YBROO8c) is Required for Benomyl and Methotrexate Resistance in Saccharomyces
cerevisiae and its Benomyl-induced Expression is Dependent on Pdr3 Transcriptional Regulator
N. Bréco, S. Tenreiro, C. A. Viegas and |. Sa-Correia
Transformation of Candida albicans by Electroporation
M. D. De Backer, D. Maes, S. Vandoninck, M. Logghe, R. Contreras and W. H. M. L. Luyten 1 609
A Potential Role of the Cytoskeleton of Saccharomyces cerevisiae in a Functional Organization of
Glycolytic Enzymes
R. Gotz, E. Schluter, G. Shoham andF . K. Zimmermann 1 619
Cloning and Characterization of the EXG7 Gene from the Yeast Yarrowia /lipolytica
P. F. Esteban, S. Casarégola, C. R. Vazquezd e Aldana and F. del Rey 1 631
Trans-dominant Mutations in the GPR? Gene Cause High Sensitivity to Acetic Acid and Ethanol in the
Yeast Yarrowia lipolytica
K. Tzschoppe, A. Augstein, R. Bauer, S. D. Kohlwein and G. Barth 1 645
inositol Synthesis and Catabolism in Cryptococcus neoformans
Y. Molina, S. E. Ramos, T. Douglass and L. S. Klig 4 657
YEAST FUNCTIONAL ANALYSIS REPORTS
A Series of Protein Phosphatase Gene Disruptants in Saccharomyces cerevisiae
N. Sakumoto, Y. Mukai, K. Uchida, T. Kouchi, J. Kuwajima, Y. Nakagawa, S. Sugioka, E. Yamamoto, T. Furuyama
H. Mizubuchi, N. Ohsugi, T. Sakuno, K. Kikuchi, |. Matsuoka, N. Ogawa, Y. Kaneko and S. Harashima 4 669
Disruption of Seven Hypothetical Ary! Alcohol Dehydrogenase Genes from Saccharomyces cerevisiae and
Construction of a Multiple Knock-Out Strain
D. Delneri, D. C. J. Gardner, C. V. Bruschi and S. G. Oliver 1681
Disruption and Phenotypic Analysis of Six Novel Genes from Chromosome !V of Saccharomyces
cerevisiae Reveal YDLO60w as an Essential Gene for Vegetative Growth
E. Casalone, C. Barberio, D. Cavalieri, |. Ceccarelli, M. Riparbelli, S. Ugolini and M. Polsinell 41 691
CURRENT AWARENESS ON YEAST 1703
ISSUE No. 16 DECEMBER 1999
Transcription of Some PHO Genes in Saccharomyces cerevisiae is Regulated by Spt7p
K. Nishimura, K. Yasumura, K. Igarashi, S. Harashima and Y. Kakinuma
RGD! Genetically Interacts with M/D2 and SLG7, Encoding Two Putative Sensors for Cell Integrity
Sgnemny in Saccharomyces cerevisiae
je Bettiqnies, C. Barthe, C. Morel, M. F. Peypouquet, F. Doignon and M. Crouzet
YEAST FUNCTIONAt ANALYSIS REPORTS
Selection of Genes Repressed by cAMP that are Induced by Nutritional Limitation in Saccharomyces
c erevisiae
Tadi, R. N. Hasan, F. Bussereau, E. Boy-Marcotte and M. Jacquet
Facilitating Functional Analysis of the Saccharomyces cerevisiae Genome Using an EGFP Based Promoter
Library and Flow Cytometry
PL. Bell, |. W. Davies and P. V. Attfield
New Tools for Protein Linkage Mapping and General Two-hybrid Screening
T. Durfee, O. Draper, J. Zupan, D. S. Conklin and P. C. Zambrysk 761
The trp1-AFA Designer Deletion for PCR-based Gene Functional Analysis in Saccharomyces cerevisiae
Horecka and Y. Jigami 1 769
Systematic Analysis of S. cerevisiae Chromosome VIII Genes
R. Niedenthal, L. Riles, U. Guldener, S. Klein, M. Johnston and J. H. Hegemann
YEAST SEQUENCING REPORTS
isolation and Characterization of MELt Gene from Torulaspora delbrueckii \FO 1255
Oda and M. Fukunaga
Calendar
Author Index
Key Word Index
Volume Contents
