Table Of ContentContents
VOLUME 14, ISSUE Nos. 1-16
Yeast
ISSUE No. 1, 15 JANUARY 1998
Sequences of Saccharomyces cerevisiae 24m DNA Improving Plasmid Partitioning in Hansenula
polymorpha
A. |. Bogdanova, O. S. Kustikova, M. O. Agaphonov and M. D. Ter-Avanesyan
Development of the Methylotrophic Yeast Pichia methanolica for the Expression of the 65 Kilodalton
isoform of Human Glutamate Decarboxylase
C. K. Raymond, T. Bukowski, S. D. Holderman, A. F. T. Ching, E. Vanaja and M. R. Stamm
Distribution of the Flocculation Protein, Flop, at the Cell Surface During Yeast Growth: The Availability
of Flop Determines the Flocculation Level
M. Bony, P. Barre and B. Blondin
The Lysine-rich C-terminal Repeats of the Centromere-binding Factor 5 (Cbf5) of Kluyveromyces /actis
are not Essential for Function
A. A. Winkler, A. Bobok, B. M. Zonneveld, H. Y. Steensma and P. J. J. Hooykaas
The Sge1 Protein of Saccharomyces cerevisiae is a Membrane-associated Multidrug Transporter
A. E. Ehrenhofer-Murray, M. U. Keller Seitz and C. Sengstag
Engineering Yeast for Efficient Cellulose Degradation
P. van Rensburg, W. H. van Zyl and| . S. Pretorius
Kluyveromyces factis SEF? and its Saccharomyces cerevisiae Homologue Bypass the Unknown
Essential Function, but not the Mitochondrial RNase P Function, of the S. cerevisiae RPM2 Gene
K. R. Groom, H. C. Heyman, M. C. Steffen, L. Hawkins and N. C. Martin
YEAST FUNCTIONAL ANALYSIS REPORTS
Efficient PCR-based Random Mutagenesis of Sub-genic (100 bp) DNA Fragments
V. Svetiov and T. G. Cooper
CURRENT AWARENESS ON YEAST
ERRATUM
ISSUE No. 2, 30 JANUARY 1998
Mutations in Five Loci Affecting GAP7-independent Uptake of Neutral Amino Acids in Yeast
M. U. Jorgensen, M. B. Bruun, T. Didion and M. C. Kielland-Brandt
Designer Deletion Strains Derived from Saccharomyces cerevisiae S288C: a Useful Set of Strains and
Plasmids for PCR-mediated Gene Disruption and Other Applications
C. B. Brachmann, A. Davies, G. J. Cost, E. Caputo, J. Li, P. Hieter and J. D. Boeke
Mutations of the CDC28 Gene and the Radiation Sensitivity of Saccharomyces cerevisiae
N. A. Koltovaya, |. P. Arman and A. B. Devin
Flow Cytometry and Cell Sorting for Yeast Viability Assessment and Cell Selection
D. Deere, J. Shen, G. Vesey, P. Bell, P. Bissinger and D. Veal
Disruption of the Saccharomyces cerevisiae YAP3 Gene Reduces the Proteolytic Degradation of
Secreted Recombinant Human Albumin
S. M. Kerry-Williams, S. C. Gilbert, L. R. Evans and D. J. Ballance
Functional Comparison of the Yeast scERV7 and scERV2 Genes
G. Stein and T. Lisowsky
YEAST SEQUENCING REPORTS
Molecular Cloning and Sequencing of a Chitin Synthase Gene (CHS2) of Paracoccidioides brasiliensis
G. A. Nifio-Vega, E. T. Buurman, G. W. Gooday, G. San-Blas and N. A. R. Gow
Characterization of a Branched-chain Amino-acid Aminotransferase from Schizosaccharomyces pombe
A. Eden and N. Benvenisty
ISSUE No. 3 FEBRUARY 1998
Investigation of Two Yeast Genes Encoding Putative lsoenzymes of Phosphoglycerate Mutase
J. J. Heinisch, S. Muller, E. Schlutter, J.J acoby and R. Rodicio
Post-translational Fate of CAN7 Permease of Saccharomyces cerevisiae
M. Opekarova, T. Caspari, B. Pinson, D. Bréthes and W. Tanner
The Saccharomyces cerevisiae TGL2 Gene Encodes a Protein with Lipolytic Activity and can
Complement an Escherichia coli Diacylglycerol Kinase Disruptant
G. P. H. van Heusden, M. Nebohacova, T. A. Overbeeke and H. Y. Steensma
isolation and Characteristics of Yeasts able to Grow at Low Concentrations of Nutrients
Y. Kimura, Y. Nakano, K. Fujita, S. Miyabe, S. Imasaka, Y. Ishikawa and M. Sato
identification and Kinetic Analysis of a Functional Homelog of Elongation Factor 3 YEF3 in Saccharo-
myces cerevisiae
A. V. Sarthy, T. McGonigal, J. O. Capobianco, M. Schmidt, S. R. Green, C. M. Moehle and R. C. Goldman
During the Initiation of Fermentation Overexpression of Hexokinase Pll in Yeast Transiently causes a
Similar Deregulation of Glycolysis as Deletion of Tps1
J). R. Ernandes, C. De Meirsman, F. Rolland, J. Winderickx, J. de Winde, R. L. Brandao and J. M. Thevelein
YEAST FUNCTIONAL ANALYSIS REPORTS
Generation of Saccharomyces cerevisiae Deletants and Basic Phenotypic Analysis of Eight Novel Genes
from the Left arm of Chromosome XIV
M. Maftahi, C. Gaillardin and J. Nicaud
YEAST SEQUENCING REPORTS
Cloning, Sequencing and Disruption of the ARG& Gene Encoding Acetylornithine Aminotransferase in
the Petite-negative Yeast K/uyveromyces /actis
A. Janssen and X. J. Chen
The SPL7 tRNA Splicing Gene of Candida maltosa and Candida albicans
E. P. Plant, D. Becher and R. T. M. Poulter
CURRENT AWARENESS ON YEAST
ISSUE No. 4, 15 MARCH 1998
Mam33p, an Oligomeric, Acidic Protein in the Mitochondrial Matrix of Saccharomyces cerevisiae is
Related to the Human Complement Receptor gC1q-R
T. Seytter, F. Lottspeich, W. Neupert and E. Schwarz
Synthesis of Monohydroxylated Inositolphosphoryiceramide (IPC-C) in Saccharomyces cerevisiae
Requires Scs7p, a Protein with both a Cytochrome b,-like Domain and Hydroxylase/desaturase Domain
T. M. Dunn, D. Haak, E. Monaghan and T. J. Beeler
Assembly of Phosphofructokinase-1 from Saccharomyces cerevisiae in Extracts of Single-Deletion
Mutants
A. Klinder, J. Kirchberger, A. Edelmanann d G. Kopperschlager
Purified Arginine Permease of Candida albicans is Functionally Active in a Reconstituted System
P. K. Mukherjee and R. Prasad
The Importance of the Glycerol 3-Phosphate Shuttle During Aerobic Growth of Saccharomyces
cerevisiae
C. Larsson, |.-L. Pahiman, R. Ansel!, M. Rigoulet, L. Adler and L. Gustafsson
The SKS71 Gene of Saccharomyces cerevisiae is Required for Long-term Adaptation of snf3 Null Strains
to Low Glucose
P. Vagnoli and L. F. Bissor
Glycosylation of Human a,-Antitrypsin in Saccharomyces cerevisiae and Methylotrophic Yeasts
H. A. Kang, J.-H. Sohn, E.-S. Choi, B. H. Chang, M.-H. Yu and S.-K. Rhee
Purification and Properties of Polyphosphatase from Saccharomyces cerevisiae Cystosol
N. Andreeva, T. Kulakovskaya, |. Sidorov, A. Karpov and |. Kulaev
YEAST FUNCTIONAL ANALYSIS REPORTS
Construction of a PCR-ligated Long Flanking Homology Cassettes for Use in the Functional Analysis of
Six Unknown Open Reading Frames from the Left and Right Arms of Saccharomyces cerevisiae
Chromosome XV
B. M. Pearson, Y. Hernandaon d M. Schweizer
ISSUE No. 5, 30 MARCH 1998
Isolation and Characterization of K/uyveromyces marxianus Mutants Deficient in Malate Transport
O. Queirés, M. Casal, S. Althoff, P. Moradas-Ferreira and C. Leao
Identification and Analysis of Homologues of Saccharomyces cerevisiae Spt3 Suggest Conserved
Functional Domains
J. M. Madison and F. Winston
Heat Shock Transiently Enhances the Synthesis Rate of Sisip, a Ribosome-Associated DnaJ Protein in
the Oleagenous Yeast Apiotrichum curvatum
V. Specht, M. Lubeck and H. Kindl
Application of mRNA Differential Display to Investigate Gene Expression in Thermotolerant Cells of
Saccharomyces cerevisiae
C. Gross and K. Watson
Evolution of Gene Order and Chromosome Number in Saccharomyces, Kluyveromyces and Related
Fungi
R. S. Keogh, C. Seoighe and K. H. Wolfe
Regulation of Alcoholic Fermentation in Batch and Chemostat Cultures of Kluyveromyces /actis CBS
2359
J. Kiers, A.-M. Zeeman, M. Luttik, C. Thiele, J. |. Castrillo, H. Y. Steensma, J. P. van Dijken and J. T. Pronk
YEAST FUNCTIONAL ANALYSIS REPORTS
The List of Cytoplasmic Ribosomal Proteins of Saccharomyces cerevisiae
R. J. Planta andW . H. Mager
YEAST SEQUENCING REPORTS
Nucleotide Sequence of the Schizosaccharomyces pombe lys1+ Gene and Similarities of the lys1+
Protein to Peptide Antibiotic Synthetases
V. Bhattacherjee and J. K. Bhattacharjee
Characterization of the Prk1 Protein Kinase from Schizosaccharomyces pombe
P. Watson and J. Davey
CURRENT AWARENESS ON YEAST
ISSUE No. 6 APRIL 1998
Influence of Monovalent Cations on Yeast Cytoplasmic and Vacuolar pH
M. Calahorra, G. A. Martinez, A. Hernandez-Cruz and A. Pena
Induced Expression of the Candida albicans Multidrug Resistance Gene CDA7 in Response to
Fluconazole and Other Antifungals
M. L. Hernaez, C. Gil, J. Pla and C. Nombela
Identification of YHRO719 in Saccharomyces cerevisiae Chromosome VIII as the Gene for the Cytosolic
Asparaginyl-tRNA Synthetase
|. Landrieu, M. Vandenbol, R. Leberman, D. Portetellaen d M. Hartlein
Deletion of Transmembrane Domain 12 of CDA7, a Multidrug Transporter from Candida albicans, Leads
to Altered Drug Specificity: Expression of a Yeast Multidrug Transporter in Baculovirus Expression
System
S. Krishnamurthy, U. Chatterjee, V. Gupta, R. Prasad, P. Das, P. Snehlata, S. E. Hasnain and R. Prasad
A Family of Laboratory Strains of Saccharomyces cerevisiae Carry Rearrangements Involving
Chromosomes | and !"!
S. Casaregola, H. Vang Nguyen, A. Lepingle, P. Brignon, F. Gendre and C. Gaillardin
YEAST FUNCTIONAL ANALYSIS REPORTS
An Improved Protocal for the Preparation of Yeast Cells for Transformation by Electroporation
J. R. Thompson, E. Register, J. Curotto, M. Kurtz and R. Kelly
YEAST SEQUENCING REPORTS
Cloning and Sequencing of a cDNA Encoding a Copper-Zinc Superoxide Dismutase Enzyme from the
Marine Yeast Debaryomyces hansenii
N. Y. Hernandez-Saavedra, J. M. Egly and J. L. Achoa
Homolog of a-factor Receptor Gene in Saccharomyces exiguus
K.-1. Kusumoto, A. Hino, Y. Yonekura, Y. Nogata, H. Ohta and H. Takano
A 9359 bp Fragment from the Right Arm of Saccharomyces cerevisiae Chromosome VII Includes the
FOL2 and YTA7 Genes and Three Unknown Open Reading Frames
M. L. Agostoni Carbone, G. Lucchini, P. Melchioretto, V. Nardese, M. Vanoni and L. Panzeri
CALENDAR
CURRENT AWARENESS ON YEAST
ISSUE No. 7 MAY 1998
Physical Mapping of Chromosomes VIi and XV of Saccharomyces cerevisiae at 3.5kb Average
Resolution to Allow their Complete Sequencing
H. Tettelin, A. Thierry, A. Goffeau and B. Dujon
An Extracellular Meiosis-promoting Factor in Saccharomyces cerevisiae
M. Hayashi, K. Ohkunanid |. Yamashita
Bicarbonate-mediated Social Communication Stimulates Meiosis and Sporulation of Saccharomyces
cerevisiae
K. Ohkuni, M. Hayashanid |. Yamashita
The Saccharomyces cerevisiae Early Secretion Mutant tip20 is Synthetic Lethal with Mutants in Yeast
Coatomer and the SNARE Proteins Sec22p and Ufelp
G. Frigerio
isolation of the Pichia pastoris PYC1 Gene Encoding Pyruvate Carboxylase and Identification of a
Suppressor of the pyc Phenotype
J. Menendez, J. Delgado and C. Gancedo
YEAST FUNCTIONAL ANALYSIS REPORTS
Genomic Disruption of Six Budding Yeast Genes gives one Drastic Example of Phenotype
Strain-dependence
E. Bilsland, M. Dahlén and P. Sunnerhagen
YEAST SEQUENCING REPORTS
identification of a Putative Histidine Kinase Two-component Phosphorelay Gene (CaHK7) in Candida
albicans
J. A. Calera, G.H. Choi and R. A. Calderone
Isolation and Characterization of the Candida albicans SEC4 Gene
M. Clément, H. Fournier, L. de Repentigny and P. Belhumeur
Genetic Organization and Sequence Analysis of the Hypha-specific Cell Wall Protein Gene HWP7 of
Candida albicans
J. F. Staab and P. Sundstrom
The H/S4 Gene from the Yeast K/luyveromyces /actis
M. A. Freire-Picos, M. Hampsey and M. E. Cerdan
CURRENT AWARENESS ON YEAST
ISSUE No. 8, 15 JUNE 1998
Pombe: A Gene-finding and Exon-intron Structure Prediction System for Fission Yeast
T. Chen and M. Q. Zhang
Transcriptional Co-regulation of Saccharomyces cerevisiae Alcohol Acetylitransferase Gene, ATF7 and
\-9 Fatty Acid Desaturase Gene, OLE7 by Unsaturated Fatty Acids
D. Fujiwara, H. Yoshimoto, H. Sone, S. Harashima and Y. Tamai
Comparison of the Structural Characteristics of Chromosome VI in Saccharomyces Sensu Stricto: The
Divergence, Species-Dependent Features and Uniqueness of Saké Yeasts
A. Nakazato, T. Kadokura, M. Amano, T. Harayama, Y. Murakami, M. Takeda, M. Ohkuma, T. Kudo andT . Kaneko
The C-terminal Hydrophobic Repeat of Schizosaccharomyces pombe Heat Shock Factor is not Required
for Heat-induced DNA-binding
K. A. Saltsman, H. L. Prentice and R. E. Kingston
Functional Analysis of the Saccharomyces cerevisiae UBC11 Gene
F. M. Townsley and J. V. Ruderman
The Pichia pastoris Dihydroxyacetone Kinase is a PTS1-containing, but Cytosolic, Protein that is
Essential for Growth on Methanol
G. H. Luers, R. Advani, T. Wenzel and S. Subramani
N-Glycosylation by Transfer of GIcNAc, from Dolichol-PP-G1cNAc, to the Protein Moiety of the Major
Yeast Exoglucanase
R. Cueva, C. Cotano and G. Larriba
YEAST FUNCTIONAL ANALYSIS REPORTS
A Versatile Set of Vectors for Constitutive and Regulated Gene Expression in Pichia pastoris
|. B. Sears, J. O'Connor, O. W. Rossanese and B. S. Glick
CALENDAR
ERRATUM...
ISSUE No. 9, 30 JUNE 1998
A Novel Esterase from Saccharomyces carlsbergensis, a Possible Function for the Yeast 7/P7 Gene
M. W. Horsted, E. S. Dey, S. Holmberg and M. C. Kielland-Brandt
Development of a Transformation System for the Multinuclear Yeast Dipodascus (Endomyces)
magnusii
L. Adamikova, P. Griaé, L. TomaSka and J. Nosek
Quantitative Imaging of TATA-Binding Protein in Living Yeast Cells
G. H. Patterson, S. C. Schroeder, Y. Bai, P. A. Weil and D. W. Piston
Highly Efficient Assimilation of Lactose by a Metabolically Engineered Strain of Saccharomyces
cerevisiae
M. Rubio-Texeira, J. |. Castrillo, A. C. Adam, U. O. Ugalde and J. Polaina
ARH1 of Saccharomyces cerevisiae: A New Essential Gene That Codes for a Protein Homologous to the
Human Adrenodoxin Reductase
L. Manzella, M. H. Barros and F. G. Nobrega
Curing Saccharomyces cerevisiae of the 2 Micron Plasmid by Targeted DNA Damage
E. L. Tsalik and M. R. Gartenberg
YEAST FUNCTIONAL ANALYSIS REPORTS
The Deletion of Six ORFs of Unknown Function From Saccharomyces cerevisiae Chromosome VII
Reveals Two Essential Genes: YGR795w and YGR198w
J. M. Rodriguez-Pena, V. J. Cid, M. Sanchez, M. Molina, J. Arroyo and C. Nombela
YEAST SEQUENCING REPORTS
Cloning and Sequence Analysis of the Pichia pastoris TRP1, IPP1 and H/IS3 Genes
1. C. Cosano, P. Alvarez, M. Molina and C. Nombela
identification and Characterization of the KLCMD7 Gene Encoding Kluyveromyces /actis Calmodulin
T. F. Rayner and M. J. R. Stark
CURRENT AWARENESS ON YEAST
ISSUE No. 10 JULY 1998
Differentiation of Brewing Yeast Strains by Pyrolysis Mass Spectrometry and Fourier Transform
infrared Spectroscopy
E. M. Timmins, D. E. Quain and R. Goodacre
Chromosomal DNA Patterns and Gene Stability of Pichia pastoris
H. Ohi, N. Okazaki, S. Uno, M. Miura and R. Hiramatsu
Control of Division Arrest and Entry into Meiosis by Extracellular Alkalisation in Saccharomyces
cerevisiae
M. Hayashi, K. Ohkuni and |. Yamashita
Genetic Interaction Between YP76 and YPT7 in Saccharomyces cerevisiae
B. Li and J. R. Warner
Co-existence of Two Types of Chromosomes in the Bottom Fermenting Yeast, Saccharomyces pastorianus
Y. Tamai, T. Momma, H. Yoshimoto and Y. Kaneko
YEAST FUNCTIONAL ANALYSIS REPORTS
Drug-induced Phenotypes Provide a Tool for the Functional Analysis of Yeast Genes
H. Launhardt, A. Hinnen and T. Munder
Heterologous Modules for Efficient and Versatile PCR-based Gene Targeting in Schizosaccharomyces
pombe
J. Bahler, J. Wu, M. S. Longtine, N. G. Shah, A. McKenzie Ill, A. B. Steever, A. Wach, P. Philippsen and J. R. Pringle
Additional Modules for Versatile and Economical PCR-based Gene Deletion and Modification in
Saccharomyces cerevisiae
M. S. Longtine, A. McKenzie III, D. J. DeMarini, N. G. Shah, A. Wach, A. Brachat, P. Philippsen and J. R. Pringle
YEAST SEQUENCING REPORTS
isolation and Nucleotide Sequence of the Gene Encoding Phosphoenolpyruvate Carboxykinase from
Kluyveromyces /actis
H. K. Kitamoto, S. Ohmomo and Y. limura
CURRENT AWARENESS ON YEAST
ISSUE No. 11 AUGUST 1998
REVIEW
The Structure and Function of Yeast Xylose (Aldose) Reductases
H. Lee
Multiple Regulatory Proteins Mediate Repression and Activation by Interaction With the Yeast Mig1
Binding Site
J. Wu and R. J. Trumbly
Suppression of sdh? Mutations by the SDH1b Gene of Saccharomyces cerevisiae
G. Colby, Y. Ishii and A. Tzagoloff 1001
Studies of Astaxanthin Biosynthesis in Xanthophyllomyces dendrorhous (Phaffia rhodozyma). Effect of
Inhibitors and Low Temperature
L. M. Ducrey Santopietro and M.-R. Kula
AILV1 Gene from the Yeast Arxu/a adeninivorans LS3—a New Selective Transformation Marker
T. Wartmann, H. Rosel, |. Kunze, R. Bode and G. Kunze
YEAST FUNCTIONAL ANALYSIS REPORTS
Functional Analysis of Three Adjacent Open Reading Frames from the Right Arm of Yeast
Chromosome XVI
B. WaSkiewicz-Staniorowska, J. Skata, M. Jasinski, M. Grenson, A. Goffeau and S. Utaszewski
A Search in the Genome of Saccharomyces cerevisiae for Genes Regulated via Stress Response Elements
E. Moskvina, C. Schuller, C. T. C. Maurer, W. H. Mager and H. Ruis
YEAST SEQUENCING REPORTS
The Uracil Permease of Schizosaccharomyces pombe: A Representative of a Family of 10 Transmembrane
Helix Transporter Proteins of Yeasts
J. de Montigny, M. L. Straub, R. Wagner, M. L. Bach and M. R. Chevallier
CURRENT AWARENESS ON YEAST
ISSUE No. 12, 15 SEPTEMBER 1998
REVIEW
Candida rugosa Lipases: Molecular Biology and Versatility in Biotechnology
S. Benjamin and A. Pandey
Transient mRNA Responses in Chemostat Cultures as a Method of Defining Putative Regulatory Elements:
Application to Genes Involved in Saccharomyces cerevisiae Acety|-coenzyme A Metabolism
M. A. van den Berg, P. de Jong-Gubbels and H. Y. Steensma
A Highly Conserved Intraspecies Homolog of the Saccharomyces cerevisiae Elongation Factor-3 Encoded
by the HEF3 Gene
T. C. Maurice, C. E. Mazzucco, C. S. Ramanathan, B. M. Ryan, G. A. Warr and J. W. Puziss
Secretion and pH-Dependent Self-Processing of the Pro-Form of the Yarrowi/a lipolytica Acid Extra-
cellular Protease
R. K. McEwan and T. W. Young
YEAST FUNCTIONAL ANALYSIS REPORTS
Functional Analysis of Yeast Essential Genes Using a Promoter-Substitution Cassette and the
Tetracycline-regulatable Dual Expression System
G. Belli, E. Gari, M. Aldea and E. Herrero
New Constructs and Strategies for Efficient PCR-based Gene Manipulations in Yeast
O. Puig, B. Rutz, B. G. Mattias Luukkonen, S. Kandels-Lewis, E. Bragado-Nilsson and B. Séraphin
YEAST SEQUENCING REPORTS
Cloning and Sequence of a 3:835 KBP DNA Fragment Containing the H/S4 Gene and a Fragment of a
PEX5-Like Gene From Candida albicans
F. Navarro-Garcia, R. Pérez-Diaz, A. |. Negredo, J. Pla and C. Nombela
ISSUE No. 13, 30 SEPTEMBER 1998
A Spheroplast Rate Assay for Determination of Cell Wall Integrity in Yeast
R. Ovalle, S. T. Lim, B. Holder, C. K. Jue, C.W ood Moore and P. N. Lipke
Characterization of a Second Gene (ZSOD22) of Na* H* Antiporter from Salt-tolerant Yeast Zygo-
saccharomyces rouxii and Functional Expression of ZSOD2 and ZSOD22 in Saccharomyces cerevisiae
T. lwaki, Y. Higashida, H. Tsuji, Y. Tamai and Y. Watanabe
Regulation of Peroxisomal Proteins and Organelle Proliferation by Multiple Carbon Sources in the
Methylotrophic Yeast, Candida bo/dinii
Y. Sakai, H. Yurimoto, H. Matsuo and N. Kato
Fluorescent Probing of Membrane Potential in Walled Cells: diS-C, (3) Assay in Saccharomyces cerevisiae
D. GaSkova, B. Brodska, P. Herman, J. Veée7, J. Malinsky, K. Sigler, O. Benada andJ . PlaSek
YEAST FUNCTIONAL ANALYSIS REPORTS
Disruption of Six Saccharomyces cerevisiae Genes from Chromosome IV and Basic Phenotypic Analysis of
Deletion Mutants
M. Carmen Lopez, M. Sanchez, E. Ferminan and A. Dominguez
Transcriptional Profiling on all Open Reading Frames of Saccharomyces cerevisiae
N. C. Hauser, M. Vingron, M. Scheideler, B. Krems, K. Hellmuth, K.-D. Entian and J. D. Hoheisel
YEAST SEQUENCING REPORTS
Cloning and Sequence Analysis of the Gene Encoding Invertase (/NV7) from the Yeast Candida utilis
F. P. Chavez. T. Pons, J. M. Delgado and L. Rodriguez
Cloning and Characterization of the Hansenula polymorpha Homologue of the Saccharomyces cerevisiae
PMRI1 Gene
H. A. Kang, J.-Y. Kim, S.-M. Ko, C. S. Park, D. D. Y. Ryu, J.-H. Sohn, E.-S. Choi and S.-K. Rhee
CURRENT AWARENESS ON YEAST
ISSUE No. 14 OCTOBER 1998
Stabilization of Two Families of Critical Targets for Hyperthermic Cell Killing and Acquired Thermo-
tolerance of Yeast Cells
K. Obuchi, H. lwahashi, J. R. Lepock and Y. Komatsu
Cloning of the Candida a!bicans Nucleoside Transporter by Complementation of Nucleoside Transport-
deficient Saccharomyces
S. Detke
Comparison of Expression Systems in the Yeasts Saccharomyces cerevisiae, Hansenula polymorpha,
Klyveromyces lactis, Schizosaccharomyces pombe and Yarrowia lipolytica. Cloning of Two Novel
Promoters from Yarrowia lipolytica
S. Muller, T. Sandal, P. Kamp-Hansen and H. Dalboge
Characterization of Deletion Mutations in the Carboxy-terminal Peptide-binding Domain of the Kar2
Protein in Saccharomyces cerevisiae
M. Tokunaga, S. Kato, A. Kawamura-Watabe, R. Tanaka and H. Tokunaga
YEAST FUNCTIONAL ANALYSIS REPORTS
A New Method for Quantitative Determination of Polysaccharides in the Yeast Cell Wall. Application to
the Cell Wall Defective Mutants of Saccharomyces cerevisiae
N. Dallies, J. M. Francois and V. Paquet
YEAST SEQUENCING REPORTS
Molecular Cloning and Sequence Analysis of the Schizosaccharomyces pombe ade10° Gene
C. Liedtke and H. Schmidt
Molecular Cloning of Alcohol Dehydrogenase Genes of the Yeast Pichia stipitis and |Identification of the
Fermentative ADH
Passoth, B. Schafer, B. Liebel, T. Weierstall and U. Klinner
Single-read Sequence Tags of a Limited Number of Genomic DNA Fragments Provide an Inexpensive Tool
for Comparative Genome Analysis
K. Hartung, D. Frishman, A. Hinnen and S. Wolfi
CURRENT AWARENESS ON YEAST
ISSUE No. 15 NOVEMBER 1998
inter- and Intraspecific Chromosome Pattern Variation in the Yeast Genus K/uyveromyces
C. Belloch, E. Barrio, M. D. Garcia and A. Querol
Monovalent Cation Fluxes and Physiological Changes of Debaryomyces hansenii Grown at High Concen-
trations of KCI and NaCl
P. E. Thomé-Ortiz, A. Peha and J. Ramirez
Cloning and Characterization of the Peroxisomal Acyl CoA Oxidase ACO3 Gene from the Alkane-utilizing
Yeast Yarrowia lipolytica
H. Wang, A. Le Clainche, M.-T. Le Dall, Y. Wache, Y. Pagot, J.-M. Belin, C. Gaillardin and J.-M. Nicaud
Cloning and Characterization of an n-alkane-inducible Cytochrome P450 Gene Essential for n-Decane
Assimilation by Yarrowi/a lipolytica
T. lida, A. Ohta and M. Takagi
YEAST SEQUENCING REPORTS
Isolation and Sequence Analysis of the Orotidine-5'-phosphate Decarboxylase Gene (URA3) of Candida
utilis. Comparison with the OMP Decarboxylase Gene Family
L. Rodriguez, F. P. Chavez, M. E. Gonzalez, L. Basabe and T. Rivero 1399
YEAST FUNCTIONAL ANALYSIS REPORTS
Vectors for Transcription Factor Cloning and Target Site Identification by Means of Genetic Selection
in Yeast
A. H. Meyer, P. B. F. Quwerkerk and J. H. C. Hoge
Quantitative Analysis of Yeast Gene Function by Using Competition Experiments in Continuous Culture
F. Baganz, A. Hayes, R. Farquhar, P. R. Butler, D. C. J. Gardner and S. G. Oliver
CURRENT AWARENESS ON YEAST
ISSUE No. 16, YEAST 1998, DECEMBER 1998
Publisher's Note
List of Referees
EDITORIAL
This Year in YEAST
R. B. Wickner
REVIEWS
A History of Research on Yeasts 1: Work by Chemists and Biologists 1789-1850
J. A. Barnett 1439
Expanding Yeast Knowledge Online
K. Dolinski, C. A. Ball, S. A. Chervitz, S. S. Dwight, M. A. Harris, S. Roberts, T. Y. Roe, J. M. Cherry and D. Botstein 1453
Biochemistry, Cell Biology and Molecular Biology of Lipids of Saccharomyces cerevisiae
G. Daum, N. D. Lees, M. Bard and R. Dickson 1471
Oxidative Stress Responses of the Yeast Saccharomyces cerevisiae
D. J. Jamieson 1511
Fusion of a Fission Yeast
J. Davey 1529
Author Index 1567
Keyword Index 1571
Volume Contents
