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About XTT and Yeast
This document summarizes new insights into the cellular reduction of tetrazolium dyes, which are widely used tools in cell biology. Key findings include: - Tetrazolium dyes like MTT that form insoluble formazans can be reduced intracellularly by NADH and in non-mitochondrial areas like the cytoplasm and plasma membrane. - Their positive charge facilitates cellular uptake via the plasma membrane potential. - Newer dyes like XTT, MTS, and WST-1 are negatively charged and reduced extracellularly via electron transport across the plasma membrane rather than intracellularly. - These unexpected findings prompt a re-evaluation of how tetrazolium dyes
Detailed Information
| Author: | ['Jay Steele'] |
|---|---|
| Publication Year: | 2018 |
| Pages: | 27 |
| Language: | English |
| Format: | |
| Price: | FREE |
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