Working with Stem Cells

381 Pages·2016·10.721 MB·English

by  Henning Ulrich, Priscilla Davidson Negraes (auth.)

Henning Ulrich ·
Priscilla Davidson Negraes
Working
with Stem
Cells
Working with Stem Cells

Henning Ulrich • Priscilla Davidson Negraes
Working with Stem Cells
A Quick and Easy Approach
of Methodologies and Applications
Henning Ulrich Priscilla Davidson Negraes
Department of Biochemistry Department of Biochemistry
Institute of Chemistry Institute of Chemistry
University of Sao Paulo University of São Paulo
São Paulo , SP , Brazil São Paulo , SP , Brazil
ISBN 978-3-319-30580-6 ISBN 978-3-319-30582-0 (eBook)
DOI 10.1007/978-3-319-30582-0
Library of Congress Control Number: 2016945374
© Springer International Publishing Switzerland 2016
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Pref ace
We are in a unique moment in human history where stem cells, previously viewed
as a great promise in medicine, are now slowly moving into clinical trials. Scientists
are now confi dent to start testing different stem cell-based protocols in humans. For
the fi rst time, we will be the pioneers, having the opportunity to do it right, setting
the example for the generations to come. Contrary to conventional medicine, cel-
lular therapies will replace damaged tissues and stay there for the lifespan of a per-
son. Alternatively, stem cells can be used as a tool to recapitulate human development
and inform us about the disease pathology, proving opportunities for novel diagnos-
tics and therapies. This is an extraordinary example of plasticity, never seen before
in medical history. The fundamental aspects of stem cells come back to the methods
that were developed over the years to reach to this point. We have learnt how to
isolate, grow, enrich, and differentiate stem cells. These methods are still evolving
in the scientifi c laboratories all over the world and will be optimized for the differ-
ent medical strategies. This book summarizes the most current and important meth-
ods, and state-of-the art protocols to manipulate stem cells. It should not be seen as
a fi xed textbook, but a snapshot of a new and dynamic era in medicine.
San Diego, CA, USA Alysson Renato Muotri, Ph.D.
v

Contents
1 Stem Cells: Principles and Applications ............................................... 1
Ágatha Oliveira , Juliana da Cruz Corrêa-Velloso , Talita Glaser ,
and Henning Ulrich
2 Human Embryonic Stem Cell Line Derivation .................................... 15
Simone Aparecida Siqueira Fonseca , Roberta Montero Costas ,
and Lygia V. Pereira
3 Adipose-Derived Mesenchymal Stromal Cells ..................................... 37
Amanda Faria Assoni , Giuliana Castello Coatti ,
Juliana Plat Aguiar Gomes , Mayra Vitor Pelatti , and Mayana Zatz
4 Neural Differentiation of Rodent Neural Progenitor Cells
and Isolation and Enrichment of Human Neural
Progenitor/Stem Cells ............................................................................. 57
Antonio H. Martins , Jose L. Roig-Lopez , and Maxine Nicole Gonzalez
5 Mice Post-natal Subventricular Zone Neurospheres:
Derivation, Culture, Differentiation and Applications ........................ 79
Laura Sardá-Arroyo , Clarissa Schitine , Sara Alves Xapelli ,
and Henning Ulrich
6 Very Small Embryonic Like Stem Cells (VSELs)
and Their Hematopoietic Specification ................................................. 97
Malwina Suszynska , Mariusz Z. Ratajczak , and Janina Ratajczak
7 Neural Crest Stem Cell Cultures: Establishment,
Characterization and Potential Use....................................................... 111
Andréa Gonçalves Trentin , Ricardo Castilho Garcez ,
and Raul Bardini Bressan
vii
viii Contents
8 Cancer Stem Cells: Issues with In Vitro Expansion
and Model Systems ................................................................................. 127
Khadidiatou Guiro , Garima Sinha , Oleta Sandiford ,
Treena L. Arinzeh , and Pranela Rameshwar
9 Spontaneous Generation of Patient-Specific Retinal
Pigment Epithelial Cells Using Induced Pluripotent
Stem Cell Technology .............................................................................. 143
David A. Carter , Britta Nommiste , Pete J. Coffey ,
and Amanda-Jayne F. Carr
10 Differentiation of Human Pluripotent Stem Cells
into Cortical Neurons.............................................................................. 163
Cassiano Carromeu , Alexandre Vessoni , Ana Paula Diniz Mendes ,
and Patricia Cristina Baleeiro Beltrão-Braga
11 Motor Neuron Differentiation from Pluripotent Stem Cells:
Development of the Technique, Synopsis of Protocols,
and Summary of Current Applications ................................................ 181
Helen Cristina Miranda and Albert R. La Spada
12 Derivation of Dopaminergic Neurons from Human Pluripotent
Stem Cells Using a Defined System and/or Small Molecules .............. 203
Atossa Shaltouki
13 Differentiation of hiPSC-Derived Cardiomyocytes .............................. 219
Fabian Zanella and Farah Sheikh
14 Endoderm Differentiation from Human Pluripotent Stem Cells ....... 237
Nathan Kumar , David Brafman , and Karl Willert
15 Pancreatic Differentiation from Human Pluripotent Stem Cells ....... 257
Nicholas Vinckier , Jinzhao Wang , and Maike Sander
16 Efficient Generation of Skeletal Myogenic Progenitors
from Human Pluripotent Stem Cells ..................................................... 277
Jaemin Kim , Alessandro Magli , and Rita C. R. Perlingeiro
17 Genome Editing in Stem Cells ............................................................... 287
Leon Tejwani , Cleber A. Trujillo , Charles A. Thomas ,
and Alysson R. Muotri
18 Neural Stem Cells: Functional Multipotency
and Spinal Cord Injury Research Protocols ......................................... 311
Yang D. Teng , Xiang Zeng , Inbo Han , and Jaime E. Anderson
Contents ix
19 Stem Cells and Tissue Engineering ....................................................... 331
Fernanda Maria Policarpo Tonelli , Nicole de Cássia Oliveira Paiva ,
Rebecca Vasconcellos Botelho de Medeiros , Mauro Cunha Xavier Pinto ,
Flávia Cristina Policarpo Tonelli, and Rodrigo Ribeiro Resende
20 Scaffolds for Embryonic Stem Cell Growth and Differentiation ........ 347
Ana Teresa Semeano , Talita Glaser , Henning Ulrich ,
and Denise Freitas Siqueira Petri
Index ................................................................................................................. 367