Table Of ContentContributors to Volume 316
elcitrA srebmun era ni sesehtnerap gniwollof the seman of .srotubirtnoc
snoitailiffA listed era .tnerruc
NITUOMJAN G. ABDULAEV (6), Center for NEELHTAK BOESZE-BATrAGLIA (5), Depart-
Advanced Research in Biotechnology, Na- ment of Molecular Biology, School of Os-
tional Institute of Standards and Technol- teopathic Medicine, University of Medicine
ogy and University of Maryland Biotechnol- and Dentistry of New Jersey, Stratford, New
ogy Institute, Rockville, Maryland 20850 Jersey 48080
GREGORY M. ACLAND (50), James A. Baker DEAN BOK (26), Jules Stein Eye Institute, Uni-
Institute, Cornell University, Ithaca, New versity of California, Los Angeles, Califor-
York 1046-35841 nia 59009
JAMES B. AMES (8), Center for Advanced Re- BABAK BORHAN (28), Department of Chemis-
search in Biotechnology, University of try, Michigan State University, East Lan-
Maryland Biotechnology Institute, Rock- sing, Michigan 42884
ville, Maryland 20850
NASUS E. FFOHREKCORB (36), Department of
VIBHA ANAND (50), .F M. Kirby Center, Biochemistry, University of Washington
Scheie Eye Institute, University of Pennsyl- School of Medicine, Seattle, Washington
vania School of Medicine, Philadelphia, 59189
Pennsylvania 9606-40191
J. DARIN NOSNORB (34), Moran Eye Center,
GNAGFLOW BAEHR (34), Moran Eye Center, University of Utah, Salt Lake City, Utah
University of Utah, Salt Lake City, Utah 23148
23148
EINNAEJ CHEN (11), Departments of Ophthal-
ROBERT B. BARLOW (3), Department of Oph- mology and Cell and Neurobiology, Mary
thalmology, State University of New York D. Allen Laboratory for Vision Research,
Health Science Center, Syracuse, New Doheny Eye Institute, University of South-
York 13210 ern California School of Medicine, Los
Angeles, California 33009
LINDA K. BARTHEL (39), Department of Anat-
omy and Cell Biology, University of Michi- Pu CHEN (27), Department of Craniofacial Bi-
gan Medical School, Ann Arbor, Michigan ology, University of Southern California
6160-90184 School of Dentistry, Los Angeles, Califor-
nia 33009
ARTIHCUS BATNI (4), Neuroscience Research
Institute, University of California, Santa GNIMIHS CHEN (40), Department of Ophthal-
Barbara, California 93117 mology and Visual Science, Washington
University School of Medicine, .tS Louis,
JEAN BENNETr (50), .F M. Kirby Center,
Missouri 63110
Scheie Eye Institute, University of Pennsyl-
vania School of Medicine, Philadelphia, ARTUR V. NAYICEDIC (41), Scheie Eye Insti-
Pennsylvania 9606-40191 tute, University of Pennsylvania, Philadel-
phia, Pennsylvania 19104
DAVID G. BIRCH (13), Retina Foundation of
the Southwest, and Department of Ophthal- M. CARTER LLAWNROC (14), Department of
mology, University of Texas Southwestern Physiology, Boston University School of
Medical School, Dallas, Texas 13257 Medicine, Boston, Massachusetts 81120
xi
xii CONTRIBUTORS TO VOLUME 316
RIMAS S. DEEB (43), Departments of Medicine YROGERG G. NIWRAG (19, 23, 38), Depart-
and Genetics, University of Washington, Se- ment of Ophthalmology, University of
,eltta Washington 98196 Washington School of Medicine, ,elttaeS
Washington 5846-59189
RETEP B. RELIWTED (9), Department of Physi-
ology and Biophysics, University of Wash- ODRACIR L. GEE (7), Department of Ophthal-
ington, Seattle, Washington 59189 mology and Visual ,secneicS Madison Med-
ical School, University of Wisconsin, Madi-
YLREBMIK A. RESNERD (49), Department of
son, Wisconsin 29735
Molecular Genetics and Microbiology, Uni-
versity of Florida College of Medicine, LEAHCIM H. GELB (29), Departments of
,ellivseniaG Florida 32610 Chemistry and Biochemistry, University of
Washington, Seattle, Washington 59189
ULE NOSSKIRE (22), Ludwig Institute for Can-
cer Research, S-17177 Stockholm, Sweden HEDIRAF IHCHSAMOHG (29), Departments of
G. L. FAIN (10,14), Departments of Physiolog- Chemistry and Biochemistry, University of
laci Science and Ophthalmology, University Washington, Seattle, Washington 59189
of California, Los Angeles, California WERDNA F. X. GREBDLOG (44), Eye Research
59009 Institute, Oakland University, Rochester,
DEBORA B. FARBER (47), Jules Stein Eye Michigan 48309
Institute, University of California School MARK P. RELLEK-YARG (9), Department of
of Medicine, Los Angeles, California Physiology and Biophysics, University of
59009 Washington, Seattle, Washington 59189
TREBOR N. SSIRAF (16), Department of Oph- ALRAC B. GREEN (18), Department of Biol-
thalmology, University of Washington
ogy, University of ,ainigriV ,ellivsettolrahC
School of Medicine, Seattle, Washington
ainigriV 22903
-59189 5846
ESIO~tNARF REELESEAH (23, 24), Department
OLUAP A. FERREIRA (45), Department of
of Ophthalmology, University of Washing-
Pharmacology and Toxicology, Medical
ton School of Medicine, ,elttaeS Washington
College of Wisconsin, Milwaukee, Wiscon-
5846-59189
sin 53226
NAITSIRHC P. HAMEL (46), INSERM ~tinU
YRNEH K. W. FONG (27), Doheny Eye Insti-
,452 Neurobiologie de l'Audition, F-34090
tute, and Departments of Ophthalmology
Montpellier, France
and Molecular Microbiology, University of
Southern California School of Medicine, NAHSNEW HAO (27), Division of Biology, -laC
Los Angeles, California 33009 ifornia Institute of Technology, Pasadena,
California 52119
ENNAEJ KCIREDERF (34), Moran Eye ,retneC
University of Utah, Salt Lake City, Utah MAILLIW W. HTRIWSUAH (48, 49), Depart-
23148 ments of Ophthalmology, and Molecular
Genetics and Microbiology, University of
AKATIHSOY ADAKUF (17, 31), Department of
Florida College of Medicine, ,eUivseniaG
Biophysics and Biochemistry, Graduate
Florida 32610
School of Science, University of Tokyo,
Bunkyo-ku, Tokyo ,3300-311 Japan IKAAKAT IHSAYAH (43), Departments of Med-
icine and Genetics, University of Washing-
DAVID L. GARaERS (37), Howard Hughes
ton, Seattle, Washington 69189
Medical Institute and Department of Phar-
macology, University of Texas Southwest- DLANOD C. HOOD (13), Department of Psy-
ern Medical Center, Dallas, Texas -53257 chology, Columbia University, New York,
0509 New York 10027
CONTRIBUTORS TO VOLUME 316 xiii
SEMAJ B. YELRUH (36), Howard Hughes Med- ARDNEJAR HONIS-RAMUK (47), Jules Stein
ical Institute and Department of Biochemis- Eye Institute, University of California
try, University of Washington School of School of Medicine, Los Angeles, Califor-
Medicine, Seattle, Washington 59189 nia 59009
OKmrtSTIM IKURA (8), Division of Molecular DEREK MAL (29), Departments of Chemistry
and Structural Biology, Ontario Cancer In- and Biochemistry, University of Washing-
stitute, University of Toronto, Toronto, On- ton, Seattle, Washington 59189
tario 25G 2M9, Canada
YCNAN R. LEE (35), University of Florida
GEENG-Fu JANG (23), Department of Oph- Brain Institute, Gainesville, Florida
thalmology, University of Washington 4420-01623
School of Medicine, Seattle, Washington
SINAJ MEL (11), Departments of Ophthalmol-
59189 - 5846
ogy and Genetics, New England Medical
MING JI (4), Department of Biochemistry and Center, Tufts University School of Medi-
Molecular Biology, State University of New cine, Boston, Massachusetts 11120
York Health Science Center, Syracuse, New
DERFLA S. NlWEL (48, 49), Department of Mo-
York 01231
lecular Genetics and Microbiology, Gene
G. J. SE~rOJ (14), Department of Physiology,
Therapy Center, University of Florida Col-
Boston University School of Medicine, Bos-
lege of Medicine, Gainesville, Florida 01623
ton, Massachusetts 02118
TREBLA M. ERIUGAM (50), .F M. Kirby Cen-
IRTIMD L. VEUKAK (6), Shemyakin and Ov-
ter, Scheie Eye Institute, University of Penn-
chinnikov Institute of Bioorganic Chemis-
sylvania School of Medicine, Philadelphia,
try, Russian Academy of Sciences, Mos-
Pennsylvania 9606-40191
cow, Russia
ANABOHS .S MANI (4), Section of Genetics and
UROTAS ARUMAWAK (1), Department of Biol-
Developmental Biology, Cornell University,
ogy, Graduate School of Science, Osaka
Ithaca, New York 3072-35841
University, Toyonaka, Osaka ,3400-065
Japan NAHTAN L. MATA (25), Department of Psychi-
atry, University of Texas Southwestern
V. J. VOLAFEK (14), Department of Physiol-
Medical Center, Dallas, Texas 53257
ogy, Boston University School of Medicine,
Boston, Massachusetts 02118 OKIHAKAT MATSUDA (31), Department of
Biophysics and Biochemistry, Graduate
WEHTTAM J. YDENNEK (36), Department of
School of Science, University of Tokyo,
Biochemistry, University of Washington
Bunkyo-ku, Tokyo ,3300-311 Japan
School of Medicine, Seattle, Washington
59189 IKUYORIH OTOMUSTAM (33), Department of
YRRAB E. XONK (3, 4), Departments of Bio- Biochemistry and Molecular Biology, and
chemistry and Molecular Biology, and Oph- NSF EPSCoR Oklahoma Biotechnology
thalmology, State University of New York Network Laser Mass Spectrometry Facility,
Health Science Center, Syracuse, New University of Oklahoma Health Sciences
York 13210 Center, Oklahoma City, Oklahoma 09137
AKOAN IROMOK (33), Department of Bio- H. R. MAa~EWS (10, 14), Physiological Lab-
chemistry and Molecular Biology, Univer- oratory, University of Cambridge, Cam-
sity of Oklahoma Health Sciences Center, bridge CB2 3EG, United Kingdom
Oklahoma City, Oklahoma 09137 ANA ZEDNEM (11), Departments of Ophthal-
AJILATAN V. AVOREPONSARK (11), Depart- mology and Cell and Neurobiology, Mary
ments of Ophthalmology and Genetics, New D. Allen Laboratory for Vision Research,
England Medical Center, Tufts University Doheny Eye Institute, University of South-
School of Medicine, Boston, Massachu- ern California School of Medicine, Los
setts 11120 Angeles, California 33009
xiv CONTRIBUTORS TO VOLUME 316
HTANNAHS SBREM (42), Department of Oph- ROr-ITRA S. SNALOP (7), Department of Oph-
thalmology, Johns Hopkins University thalmology and Visual Sciences, Madison
School of Medicine, Baltimore, Maryland Medical School, University of Wisconsin,
50212 Madison, Wisconsin 53792
TREBOR S. YADLOM (44), Department of -DiB OICANGI OICNEVORP (18), Department of
chemistry and Molecular Biology, Univer- Anatomy and Cell Biology, Uniformed Ser-
sity of British Columbia, Vancouver, British vices University, Bethesda, Maryland 51802
Columbia V6T 1ZD, Canada
TREBOR R. ODNAR (20, 30), Department of
SALOHCIN AKZCYZUM (48), Department of Biological Chemistry and Molecular Phar-
Molecular Genetics and Microbiology, macology, Harvard Medical School, Bos-
Gene Therapy Center, University of Florida ton, Massachusetts 51120
College of Medicine, Gainesville, Florida
REFINNEJ A. YAR (25), Division of Life Sci-
01623
ences, University of Texas, San Antonio,
KoJI IHSINAKAN (28), Department of Chemis-
Texas 2660-94287
try, Columbia University, New York, New
ALEMAP A. DNOMYAR (39), Department of
York 10027
Anatomy and Cell Biology, University of
OCIRNE NASI (3), Department of Physiology,
Michigan Medical School, Ann Arbor,
Boston University School of Medicine, Bos-
Michigan 6160-90184
ton, Massachusetts 02118
T. LEAHCIM DNOMDER (46), Laboratory of
YMEREJ SNAHTAN (42), Departments of Mo-
Retinal Cell and Molecular Biology, Na-
lecular Biology and Genetics, Neuroscience,
and Ophthalmology, Howard Hughes Med- tional Eye Institute, National Institutes of
ical Institute, Johns Hopkins University Health, Bethesda, Maryland 29802
School of Medicine, Baltimore, Maryland NlVEK D. RIDGE (6), Center for Advanced
50212 Research in Biotechnology, National Insti-
IHSORIH ORUGHO (32), Department of Oph- tute of Standards and Technology and Uni-
thalmology, Sapporo Medical University versity of Maryland Biotechnology Institute,
School of Medicine, Chuoku, Sapporo Rockville, Maryland 20850
Hokkaido ,3458-060 Japan DERF RIEKE (12), Department of Physiology
IKUYIHSOT OKANO (17), Department of and Biophysics, University of Washington,
Biophysics and Biochemistry, Graduate Seattle, Washington 0927-59189
School of Science, University of Tokyo, NASUS W. NOSNIBOR (37), Howard Hughes
Bunkyo-ku, Tokyo ,3300-311 Japan Medical Institute and Department of Phar-
EINNA E. OTFo-BRuc (16), Department of macology, University of Texas Southwest-
Ophthalmology, University of Washington ern Medical Center, Dallas, Texas -53257
School of Medicine, Seattle, Washington 0509
5846-59189
DLANOR NAMPEOR (45), Department of Hu-
FOTZSYZRK IKSWEZCLAP (21, 23, 24, 29), De-
man Genetics, University Hospital Nip
partments of Ophthalmology, Chemistry,
megen, Nijmegen 6500 HB, The Nether-
and Pharmacology, University of Washing-
lands
ton School of Medicine, Seattle, Washington
KRAM D. GALLOR (18), Department of Anat-
5846-59189
omy and Cell Biology, Uniformed Services
GIARC k. HSIRAP (30), Department of Biolog-
University, Bethesda, Maryland 20815
ical Chemistry and Molecular Pharmacol-
ogy, Harvard Medical School, Boston, Mas- ANNA TREMOR (22), Ludwig Institute for
sachusetts 51120 Cancer Research, S-17177 Stockholm,
Sweden
DIVAD R. GREBREPPEP (13), Department of
Ophthalmology and Visual Sciences, Col- OTREBLA RUIZ (26), Department of Neu-
lege of Medicine, University of Illinois, Chi- robiology, University of California, Los
cago, Illinois 21606 Angeles, California 59009
CONTRIBUTORS TO VOLUME 316 VX
JOHN C. SAARI (19, 23, 36, 38), Departments TREBUL REYRTS (8), Department of Neurobi-
of Ophthalmology and Biochemistry, Uni- ology, Stanford University School of Medi-
versity of Washington School of Medicine, cine, Stanford, California 5215-50349
Seattle, Washington 5846-59189 DIVAD NEDGUS (18), Endocrinology and Re-
TELUAD K. VEAPTAS (2), Department of Mo- production Research Group, Physiology
lecular and Cellular Pharmacology and Division, School of Biomedical Sciences,
Neuroscience Program, University of Mi- Kings College London, London SE1 1UL,
ami School of Medicine, Miami, Florida United Kingdom
63133
DRAHCIR NOSNAWS (3), Merck, Sharpe and
DIANA KCIHCS (45), Department of Pharma- Dohme Research Laboratories, West Point,
cology and Toxicology, Medical College of
Pennsylvania 68491
Wisconsin, Milwaukee, Wisconsin 53226
LEAHCIIV1 R. TAYLOR (36), Department of
DAWD ZTLEIHCS (29), Department of Molecu-
Biochemistry, University of Washington
lar Biotechnology, University of Washing-
School of Medicine, Seattle, Washington
ton, Seattle, Washington 59189
59189
ESSIRAHC RETEULHCS (4), Department of -DiB
DEBRA A. NOSPMOHT (3), Department of
chemistry and Molecular Biology, State
Ophthalmology, University of Michigan
University of New York Health Science
Medical School, Ann Arbor, Michigan
Center, Syracuse, New York 13210
6160-90184
NASUS L. DNALWOR-ELPMES (35), University
of Florida Brain Institute, Gainesville, Flor- NEK TRAN (47), Jules Stein Eye Institute, Uni-
adi 4420-O1623 versity of California School of Medicine,
Los Angeles, California 59009
YASDNIL T. EPRAHS (42), Forschungsstelle fiir
Experimentelle Ophthalmologie, Universi- WERDNA T. C. TSIN (25), Division of Life
ti~ts-Augenklinik Abteilung II, D-72076 Sciences, University of Texas, San Antonio,
,negnibi~T Germany Texas 2660-94287
NNYL C. WAHS (49), Department of Molecular REHTAEH A. NAV EPPS (36), Department of
Genetics and Microbiology, University of Biochemistry, University of Washington
Florida College of Medicine, Gainesville, School of Medicine, Seattle, Washington
Florida 32610 59189
SARDNA NOMIS (22), Department of Biochem- LUAP R. NAV LEKNIG (7), Department of Oph-
istry and Molecular Biology, University thalmology and Visual Sciences, Madison
College London, London WC1E 6BT,
Medical School, University of Wisconsin,
United Kingdom
Madison, Wisconsin 53792
NELDALV Z. KAPELS (2), Department of Mo-
J. NOTSERP VAN RESOOH (16, 38), Department
lecular and Cellular Pharmacology and
of Ophthalmology, University of Washing-
Neuroscience Program, University of Mi-
ton School of Medicine, Seattle, Washington
ami School of Medicine, Miami, Florida
5846-59189
63133
ELIA T. ANAZALLIV (25), Division of Life Sci-
MARIA L. OTUOS (28), Universidad de La La-
ences, University of Texas, San Antonio,
guna, Santa Cruz, Mexico
Texas 2660-94287
TUMTRAH REHCETS (21), Department of Oph-
thalmology, University of Washington T. MrNH VUONO (15), Aurora Biosciences,
School of Medicine, Seattle, Washington San Diego, California 12129
5846-59189 ASERET M. REKLAW (7), Department of Oph-
WERDNA NAMKCOTS (42), Department of Psy- thalmology and Visual Sciences, Madison
chology, University of California, San Medical School, University of Wisconsin,
Diego, La Jolla, California 9010-39029 Madison, Wisconsin 29735
xvi CONTRIBUTORS TO VOLUME 316
KCIRTAP O. NELAHW (49), Department of Mo- GNIMIEW YAS (49), Department of Molecular
lecular Genetics and Microbiology, Univer- Genetics and Microbiology, University of
sity of Florida College of Medicine, Gaines- Florida College of Medicine, ,ellivseniaG
,elliv Florida 32610 Florida 32610
MARK E. WHITrEN (29), Departments of NHOJ R. SETAY III (29), Department of Mo-
Chemistry and Biochemistry, University of
lecular Biotechnology, University of Wash-
Washington, Seattle, Washington 59189
ington, Seattle, Washington 59189
SIROJ XKCIREDNIW (43), Departments of Med-
KOHEI AMAYOKOY (29), Departments of
icine and Genetics, University of Washing-
Chemistry and Biochemistry, University of
ton, Seattle, Washington 69189
Washington, Seattle, Washington 59189
Er'TENA NOTSNIW (20), Department of Bio-
logical Chemistry and Molecular Pharma- DLANOD J. ZACK (40), Departments of Oph-
cology, Harvard Medical School, Boston, thalmology, Neuroscience, and Molecular
sttesuhcassaM 02115 Biology and Genetics, Johns Hopkins Uni-
versity School of Medicine, Baltimore,
OKIHOMOT IHCUGAMAY (43), Departments of
Maryland 21287
Medicine and ,sciteneG University of Wash-
ington, Seattle, Washington 98196 IEGRES NIHKUTOLOZ (48), Department of Mo-
EDYLC K. ATIHSAMAY (47), Jules Stein Eye lecular Genetics and Microbiology, Gene
,etutitsnI University of California School of Therapy Center, University of Florida Col-
Medicine, Los Angeles, California 59009 lege of Medicine, ,ellivseniaG Florida 32610
Preface
Molecular methods related to visual transduction and the visual cycle
have grown in number and evolved considerably since they were covered
in Volume 18 of Methods in Enzymology in 1982. During this time the
fundamental principles elicited through the study of visual signal transduc-
tion have been extended far into the biology of other transduction systems
initiated by numerous types of small chemical molecules.
Unprecedented progress has been made in vision research since 1982,
and newly developed methods to study vertebrate phototransduction and
the visual cycle are summarized in this Volume 316 (Part B) of Methods
in Enzymology and its companion Volume 513 (Part A). The methods
included are very broadly defined. In this volume the methods appear
in sections on characterization of calcium-binding proteins and calcium
measurements in photoreceptor cells; methods to identify posttranslational
and chemical modifications; assays to study phototransduction in vitro and
in vivo; characterization of enzymes of the visual cycle; analysis of animal
models; and methods to study inherited retinal diseases: from the defective
gene to its function and repair.
In Volume 315 they appear in sections on characterization of opsins;
methods to study properties of proteins that interact with rhodopsin; charac-
terization of photoreceptor protein phosphatases, phosphodiesterase, and
guanylyl cyclase; cyclic nucleotide-gated channels; and Na÷/Ca2÷-K ÷ ex-
changer and ABCR transporter.
It is my pleasure to thank the authors, the reviewers, Drs. M. I. Simon
and J. N. Abelson (the editors-in-chief), and the publisher who made this
project possible. In particular, I would like to thank members of my labora-
tory for their patience and support during the hectic period of editing
these volumes.
KRZYSZTOF PALCZEWSKI
xvii
METHODS IN ENZYMOLOGY
EMULOV I. Preparation and Assay of Enzymes
Edited yb SIDNEY P. KCIWOLOC DNA NATHAN O. KAPLAN
VOLUME II. Preparation and Assay of Enzymes
Edited by SIDNEY P. KCIWOLOC DNA NATHAN O. KAPLAN
EMULOV III. Preparation and Assay of Substrates
Edited by SIDNEY P. KCIWOLOC DNA NATHAN O. KAPLAN
VOLUME IV. Special Techniques for the Enzymologist
Edited by SIDNEY P. KCIWOLOC DNA NATHAN O. KAPLAN
VOLUME V. Preparation and Assay of Enzymes
Edited by SIDNEY P. KCIWOLOC DNA NATHAN O. KAPLAN
EMULOV VI. Preparation and Assay of Enzymes (Continued)
Preparation and Assay of Substrates
Special Techniques
Edited by SIDNEY P. KCIWOLOC DNA NATHAN O. KAPLAN
VOLUME VII. Cumulative Subject Index
Edited by SIDNEY P. KCIWOLOC DNA NATHAN O. KAPLAN
EMULOV VIII. Complex Carbohydrates
Edited by ELIZABETH F. NEUFELD DNA VICTOR GRUBSNIG
EMULOV IX. Carbohydrate Metabolism
Edited by WILLIS A. WOOD
EMULOV X. Oxidation and Phosphorylation
Edited by RONALD W. KOORBATSE DNA MAYNARD E. NAMLLUP
EMULOV XI. emyznE Structure
Edited by C. H. W. HIRS
EMULOV XII. Nucleic Acids (Parts A and B)
Edited by LAWRENCE NAMSSORG DNA KIVIE EVADLOM
EMULOV XIII. Citric Acid Cycle
Edited by J. M. NIETSNEWOL
EMULOV XIV. Lipids
Edited by J. M. NIETSNEWOL
EMULOV XV. Steroids and Terpenoids
Edited by RAYMOND B. NOTYALC
EMULOV XVI. Fast Reactions
Edited by KENNETH KUSTIN
xix
XX METHODS IN ENZYMOLOGY
EMULOV XVII. Metabolism of Amino Acids and Amines (Parts A and B)
Edited by TREBREH ROBAT DNA CELIA ETIHW ROBAT
EMULOV XVIII. Vitamins and Coenzymes (Parts A, B, and C)
Edited by DLANOD B. KCIMROCcM DNA LEUMEL D. THGIRW
EMULOV XIX. Proteolytic Enzymes
Edited by EDURTREG E. NNAMLREP DNA OLZSAL DNAROL
EMULOV XX. Nucleic Acids and Protein Synthesis (Part C)
detidE yb KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXI. Nucleic Acids (Part D)
Edited yb ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXII. Enzyme Purification and Related Techniques
Edited by MAILLIW B. YBOKAJ
EMULOV XXIII. Photosynthesis (Part A)
Edited yb YNOHTNA NAS ORTEIP
EMULOV XXIV. Photosynthesis and Nitrogen Fixation (Part B)
Edited yb YNOHTNA NAS ORTEIP
EMULOV XXV. Enzyme Structure (Part B)
detidE by C. H. W. HIRS DNA EGRES N. FFEI~SAMIT
EMULOV XXVI. Enzyme Structure (Part C)
Edited yb C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVII. Enzyme Structure (Part D)
detidE by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVIII. Complex Carbohydrates (Part B)
Edited by ROTCIV GRUBSNIG
EMULOV XXIX. Nucleic Acids and Protein Synthesis (Part E)
Edited by ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXX. Nucleic Acids and Protein Synthesis (Part F)
detidE yb KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXXI. Biomembranes (Part A)
detidE yb YENDIS REHCSIELF DNA RETSEL REKCAP
EMULOV XXXIL Biomembranes (Part )B
Edited by SIDNEY FLEISCHER AND LESTER PACKER
EMULOV XXXIII. Cumulative Subject Index Volumes I-XXX
detidE yb AHTRAM G. SINNED DNA DRAWDE A. SINNED
EMULOV XXXIV. Affinity Techniques (Enzyme Purification: Part B)
detidE by MAILLIW B. YBOKAJ DNA MEIR KEHCLIW
EMULOV XXXV. Lipids (Part B)
detidE by NHOJ M. NIETSNEWOL
METHODS IN ENZYMOLOGY xxi
EMULOV XXXVI. Hormone Action (Part A: Steroid Hormones)
Edited by BERT W. O'MALLEY DNA JOEL G. NAMDRAH
EMULOV XXXVII. Hormone Action (Part B: Peptide Hormones)
Edited by BERT W. O'MALLEY DNA JOEL G. HARDMAN
EMULOV XXXVIII. Hormone Action (Part C: Cyclic Nucleotides)
Edited by JOEL G. HARDMAN DNA BERT W. O'MALLEY
EMULOV XXXIX. Hormone Action (Part D: Isolated Cells, Tissues, and Organ
Systems)
Edited by JOEL G. NAMDRAH DNA BERT W. YELLAM'O
EMULOV XL. Hormone Action (Part E: Nuclear Structure and Function)
Edited by BERT W. O'MALLEY DNA JOEL G. NAMDRAH
EMULOV XLI. Carbohydrate Metabolism (Part B)
Edited by W. A. DOOW
EMULOV XLII. Carbohydrate Metabolism (Part C)
Edited by W. A. DOOW
EMULOV XLIII. Antibiotics
Edited by NHOJ H. HSAH
EMULOV XLIV. Immobilized Enzymes
Edited by SUALK HCABSOM
EMULOV XLV. Proteolytic Enzymes (Part B)
Edited by OLZSAL DNAROL
EMULOV XLVI. Affinity Labeling
Edited by WILLIAM B. JAKOBY DNA MUIR WILCHEK
EMULOV XLVII. Enzyme Structure (Part E)
Edited by .C H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XLVIII. Enzyme Structure (Part F)
Edited by .C H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XLIX. Enzyme Structure (Part G)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV L. Complex Carbohydrates (Part C)
Edited by ROTCIV GRUBSNIG
EMULOV LI. Purine and Pyrimidine Nucleotide Metabolism
Edited by AICIRTAP A. EEFFOH DNA MARY ELLEN JONES
EMULOV LII. Biomembranes (Part C: Biological Oxidations)
Edited by YENDIS REHCSIELF DNA tESTER REKCAP
EMULOV LIII. Biomembranes (Part D: Biological Oxidations)
Edited by SIDNEY FLEISCHER DNA LUSTER PACKER
EMULOV LIV. Biomembranes (Part E: Biological Oxidations)
Edited by SIDNEY FLEISCHER DNA LUSTER PACKER
