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Transcription Factors: Methods and Protocols PDF

407 Pages·2010·8.265 MB·English
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About Transcription Factors: Methods and Protocols

In the last few years, significant breakthroughs in transcription research expanded our appreciation for the complexity of molecular controls on gene expression in mammalian cells. In Transcription Factors: Methods and Protocols, experts in the field describe state-of-the-art approaches that investigators can use to probe critical mechanisms underlying transcription factor nuclear-cytoplasmic trafficking as well as to assess the functional impact of post-translational modifications on transcription factor function. The chapters are written by prominent scientists, many of whom developed these methods, and highlight protocols that focus on specific transcription factor family members with particular relevance to human disease. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.Comprehensive and current, Transcription Factors: Methods and Protocols compiles the latest techniques for elucidating controls on transcription factor intracellular localization and activity, and consequently is unlike any other methods-based text on transcriptional regulation today.

Detailed Information

Author:Céline Charlot, Hélène Dubois-Pot, Tsvetan Serchov, Yves Tourrette, Bohdan Wasylyk (auth.), Paul J. Higgins (eds.)
Publication Year:2010
ISBN:9781607617372
Pages:407
Language:English
File Size:8.265
Format:PDF
Price:FREE
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