THESIS Submitted In partial fulfillment of the requirements for the Degree of MASTER OF

155 Pages·2015·24.53 MB·English

THERAPEUTIC MANAGEMENT OF NON-REGENERATIVE ANEMIA IN
CANINES

THESIS

Submitted

In partial fulfillment of the requirements for the Degree of

MASTER OF VETERINARY SCIENCE
IN

VETERINARY CLINICAL MEDICINE, ETHICS AND

JURISPRUDENCE

BY
INGLE CHINMAY SURENDRANATH
Enrolment No.: V/09/028

Bombay Veterinary College, Mumbai

MAHARASHTRA ANIMAL AND FISHERY SCIENCES
UNIVERSITY, NAGPUR – 440 001
(INDIA)
2016
DECLARATION OF THE STUDENT

I hereby declare that the experimental research work and interpretation

of the thesis entitled “THERAPEUTIC MANAGEMENT OF NON-

REGENERATIVE ANEMIA IN CANINES” or part thereof has not been
submitted for any of the other degree or diploma of any university, nor the data
have been derived from any thesis or publications of any university or scientific

organization. The sources of material used and all assistance received during

the course of investigation have been duly acknowledged.

Signature
Date- / /2016
(INGLE CHINMAY SURENDRANATH)

Enrolment No. V/09/028 Reg. No.

1543

Signature

(Dr. J. U. Patil)
Date - / /2016
Chairman, Advisory Committee

Assistant Professor,
Department of Veterinary

Epidemiology and Preventive Medicine

ii
DECLERATION OF ADVISORY COMMITTEE

Shri. INGLE CHINMAY SURENDRANATH has satisfactorily prosecuted
his course of research work for a period of not less than one semester and that
the thesis entitled “THERAPEUTIC MANAGEMENT OF NON-REGENERATIVE

ANEMIA IN CANINES” submitted by him is the result of research work is

sufficient to warrant its presentation to the examination in subject of Veterinary
Clinical Medicine, Ethics and Jurisprudence for the award of degree by the
Maharashtra Animal and Fishery Sciences University, Nagpur.

We also certify that the thesis or part thereof has not been previously

submitted by him for a degree of any other university.

Signature
Place: Mumbai Dr. J. U. Patil
Date: Advisor / Guide
Assistant Professor,
Department of Veterinary Epidemiology
and Preventive Medicine
ADVISORY COMM ITTEE
Name and Signature of Advisory Committee Members

Sr.
Name Designation Signature
No.

Head of departments
Dept. of Veterinary Anatomy & Histology,
1 Dr. P. L. Dhande
Physiology & Biochemistry and Animal
Nutrition

Professor
2 Dr. R. V. Gaikwad Dept. of Veterinary Clinical Medicine,

Ethics and Jurisprudence
Professor
3. Dr. S. D. Ingole
Dept. of Veterinary Physiology
Assistant Professor
4. Dr. R. D. Velhankar Dept. of Veterinary Clinical Medicine,
Ethics and Jurisprudence
Assistant Professor

5. Dr. D. A. Pawalkar Dept. of Veterinary Epidemiology and
PreventiveMedicine
iii
CERTIFICATE

This is to certify that the thesis entitled “THERAPEUTIC MANAGEMENT OF

NON-REGENERATIVE ANEMIA IN CANINES” submitted by Shri. INGLE CHINMAY
SURENDRANATH to the Maharashtra Animal Sciences University, Nagpur, in partial
fulfillment of the requirement for the degree of MASTER OF VETERINARY SCIENCE

(M. V. Sc.) has been approved by the Student’s Advisory Committee after examination in

collaboration with the External Examiner.
.

Signature

Name & Signature of (Dr. (Mrs) M. M. Gatne) ( D r . J. U. Patil)

External Examiner Head of the Department Advisor/Guide

Sr.
Name Designation Signature
No.

Head of departments
Dept. of Veterinary Anatomy & Histology,
1 Dr. P. L. Dhande
Physiology & Biochemistry and Animal
Nutrition

Professor
2 Dr. R. V. Gaikwad Dept. of Veterinary Clinical Medicine,

Ethics and Jurisprudence
Professor
3. Dr. S. D. Ingole
Dept. of Veterinary Physiology
Assistant Professor
4. Dr. R. D. Velhankar Dept. of Veterinary Clinical Medicine,
Ethics and Jurisprudence
Assistant Professor
5. Dr. D. A. Pawalkar Dept. of Veterinary Epidemiology and
Preventive Medicine

Signature with seal
Date: Dean / Associate Dean
Bombay Veterinary College
ACKNOWLEDGEMENTS
ACKNOWLEDGEMENTS
At the onset, I express my deep sense of gratitude to my
honorable teacher and research guide Dr. J. U. Patil sir, Assistant
Professor of Epidemiology and Preventive Medicine, Bombay
Veterinary College, Parel, Mumbai for his valuable guidance,
executing and pursuance of the present research project. I shall
forever remain an admirer of his interest in work, deep
knowledge, approach to the subject, confidence, love for his
students and college and his constant cooperation throughout my
entire research work and post graduation studies, all of which
make him unforgettable personality.
I sincerely express my thanks to Dr. R. V. Gaikwad sir,
Hospital superintendent, TVCC & Professor of Veterinary Clinical
Medicine, Ethics and Jurisprudence, Bombay Veterinary College,
Parel, Mumbai and Dr. R. D. Velhankar sir and Dr. D. G. Dighe
sir (Associate Professor of of Veterinary Clinical Medicine, Ethics
and Jurisprudence) from the bottom of my heart for his
constructive suggestions and painstaking efforts. His valuable
suggestions have made a great difference to my performance. I
would also like to thank to Dr. C. N. Galdhar sir, Assistant
Professor, Department of Veterinary Clinical Medicine, Ethics and
Jurisprudence for their valuable suggestions & help.
I am indebted to Dr. P. S. Masare, Dr. K. V. Garud, Dr.
Krishna Ingole for their constant inspiration, advice and untiring
cooperation throughout my entire postgraduate studies.
I extend my thanks to Dr. R. L. Dhande, Head of
departments of Veterinary Anatomy & Histology, Physiology &
Biochemistry and Animal Nutrition, Dr. S. D. Ingole, Professor
Department of Veterinary Physiology, Dr. D. A. Pawalkar for
their invaluable suggestion & guidance as a member of advisory
committee during preparation of this research work.
I am also thankful to Unique veterinary Laboratory &
Primax for providing me reports on proper time, which really
helped me in the diagnosis & treatment.
This acknowledgement would be incomplete without
thinking of my colleagues Dr. Uttareshwar Surwase, Dr. Purvi
Naik, Dr. Shivanand Biradar, Dr. Ravikumar Meshram, Dr.
Akash Vaidya, Dr. Shivkumar Yankam, Dr. Vividha Charate, Dr.
Sayli Ramteke, Dr. Trupti Wanjari, Dr. Padam & who were
always willing to help me during the entire research work. I am
also thankful to My senior Dr. Uttam sir, Dr. Nitin Sangekar sir,
Dr. Divakar sir, Dr. Ashwini sir, Dr. Manish sir, Dr. Abhineet
Rumne sir, Dr. Sachin Raut sir, Dr. Sachin Nagre sir, Dr. Akshata
Ubale, Dr. Ekta Thakkar, Dr. Varsha mam & Dr. Amruta
Parulkar.
I am also thankful to Dr. Snehal Saudagar, Dr. Tanaya Pai,
Dr. Anushka, Dr. Anil Datir, Dr. Krisna Kumar who helped me
during entire reaserch work.
I am extremely overjoyed to extend my thanks to all staff
members of medicine department viz. Mr. Shivgan, Adsul kaka
Raju kaka, Babu kaka, & Murgeshan for their extended help.
I am extremely thankful to Mangesh Salvi, Khandu, Kharie
kaka, Suresh Kamble, Dharmapal Shinde, Patange, Dalvi,
Ranjan, Kulkarni, Khaire kaka, Gundemama, Mr. Patange, Kiran
Gaikwad and all other staff of BSDPHA for helping me during my
thesis work.
I do not have proper words to pay regards to my
respected All grandfathers and grand mothers, father, mother,
brothers, Snehal Vahini and Kirti Vahini because of whom I could
read, write and come up to this stage. I am also thankful to my
angels Pranjal & ojal for their best wishes. I would like to express
my deep sense of gratitude, indebtedness towards my Parents who
took pains to bring me up to this stage. I shall be failing in duties if
I do not express gratitude towards my whole family for their love,
wishes, inspiration and blessings.
During my stay at this esteemed institute, I was fortunate
enough to receive the kind co-operation from almost everyone in
either way. It is extremely difficult to thank all of them
individually by name. This shortcoming may be pardoned.
DATE: 7/09/2016
Place: BVC, Mumbai (Chinmay Surendranth Ingle)
vi
TABLE OF CONTENTS
Sr. No. CHAPTER PAGE NO.
1. INTRODUCTION : 1 –3
2. REVIEW OF LITERATURE : 4-27
3. MATERIALS AND METHODS : 28-32
4. RESULTS AND DISCUSSION : 33-60
5. SUMMARY AND CONCLUSIONS : 61-65

A. BIBLIOGRAPHY : i-xi
B. APPENDIX : xii-xiii
C. ABSTRACT : xiv-xix
D. VITA xx
LISTS OF TABLES
Sr. No. Title Page No.
Values of HB (g%), PCV (%) and Total Reticulocyte count/µl
1 33-34
of selected cases of nonregenerative anemia
2 Detailed classification of cases in different groups 33-34
3 Breed wise and GroupWise distribution of canines 33-34
4 Age wise and Group wise distribution of canines 33-34
Details of case description, clinical manifestations and history
5 34-35
of dogs with Nonregenerative anemia of group A-1, B-1, C-1
Distribution of clinical manifestations in cases of
6 34-35
nonregenerative anemia of Groups A-1, B-1 and C-1
Details of case description, clinical manifestations and history
7 34-35
of dogs with Nonregenerative anemia of group A-2, B-2, C-2
Distribution of clinical manifestations in cases of
8 34-35
nonregenerative anemia of Groups A-2, B-2 and C-2
Details of clinical examination of dogs having Non-
9 35-36
regenerative anemia
10 The calculated dose of Inj. Iron dextran (50mg/ml) 35-36
11 Therapeutic management of dogs of Group A 35-36
12 Therapeutic management of dogs of Group B 36-37
13 Therapeutic management of dogs of Group C 37-38
Details of case description, clinical manifestations of dogs
14 with Nonregenerative anemia of group A-1, B-1, C-1 after 38-39
treatment
Details of case description, clinical manifestations of dogs
15 with Nonregenerative anemia of group A-2, B-2, C-2 after 39-40
treatment
Mean ± SE values of hematobiochemical parametersof Group
16 A-1 (n=7) on 0th day and 14th day with t test and % 39-40
increase/decrease after treatment
Mean ± SE values of hematobiochemical parametersof Group
17 B-1 (n=7) on 0th day and 14th day with t test and % 39-40
increase/decrease after treatment
Mean ± SE values of hematobiochemical parametersof Group
18 C-1 (n=7) on 0th day and 14th day with t test and % 39-40
increase/decrease after treatment
Mean ± SE values of hematobiochemical parametersof Group
19 A-2 (n=3) on 0th day and 14th day with t test and % 39-40
increase/decrease after treatment
Mean ± SE values ofhematobiochemical parametersof Group
20 B-2 (n=3) on 0th day and 14th day with t test and % 39-40
increase/decrease after treatment
Mean ± SE values of hematobiochemical parametersof Group
21 C-2 (n=3) on 0th day and 14th day with t test and % 39-40
increase/decrease after treatment

viii
LISTS OF Plates
Sr. Page
Title
No. No.
1 Drug (Inj. Iron Dextran) used for all Groups 31-32
2 Drug (Inj. Amino acid preparation) used for all Groups 31-32
3 Drug (Inj. Darbepoietinalfa) used for Groups B-1 and B-2 31-32
Drug (Inj. NandroloneDecanoate) used for Groups C-1
4 31-32
and C-2
5 Dog (Case 17) being administered fluid therapy 36-37

ix