The Potential Antioxidant and Anti-Inflammatory Effects of Date Seed Powder in Rats

107 Pages·2017·2.71 MB·English

United Arab Emirates University
Scholarworks@UAEU
Theses Electronic Theses and Dissertations
11-2016
The Potential Antioxidant and Anti-Inflammatory
Effects of Date Seed Powder in Rats
Fatima Theyab Saif Amer AI Meqbaali
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Part of theEnvironmental Sciences Commons
Recommended Citation
Amer AI Meqbaali, Fatima Theyab Saif, "The Potential Antioxidant and Anti-Inflammatory Effects of Date Seed Powder in Rats"
(2016).Theses. 473.
https://scholarworks.uaeu.ac.ae/all_theses/473
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vi

Abstract

Chronic diseases are among the main leading causes of mortality and morbidity in
the United Arab Emirates (UAE). Oxidative stress (defined as an imbalance in the
reactive oxygen species production/degradation ratio) in interaction with
inflammation, has been highlighted as one major underlying mechanism in the
development of these diseases. Due to their antioxidant and anti-inflammatory
properties, polyphenols could prevent the development of chronic diseases.
Interestingly, date seeds are particularly rich in such compounds. Nonetheless, an in
vivo study aiming at studying and understanding antioxidant and anti-inflammatory
effects of date seeds in serum and various organs is missing. Therefore, the present
preclinical study is a step further to investigate these effects of date seeds in animal.
Forty rats were fed for 13 weeks with either a control diet or a diet containing date
seed powder (DSP) (0.2%, 0.4%, 0.8%). Biomarkers of the antioxidant status,
protein and lipid oxidative damages, low-grade inflammation markers were
measured in the serum and organs and histopathology was done. DSP was shown as
not altering organs’ function, as significantly increasing the antioxidant defense
system in serum and organs and as decreasing protein and lipid oxidative damages in
organs. Besides, DSP did not alter the immune inflammation markers. This
highlights a preventive role of DSP against oxidative stress-related chronic diseases
as well as suggesting its possible anti-inflammatory effects.

Keywords: Date seeds powder, chronic disease, antioxidants, oxidative damages,
inflammation, polyphenols.
vii

Title and Abstract (in Arabic)

نارئفلا ًلع رمتلا يىن يف ةبهتللااو ةذسكلأا ثادبضمل لمتحملا ريثأتلا
صخلملا
دبٛفٕنأ ضازيلأن خٛسٛئزنا ةبجسلأا ٍٛث ٍي (chronic diseases) خُيشًنا ضازيلأا زجتؼت
(oxidative حذسكلأا خٛهًػ ٗهػ ءٕعنا طٛهست ىت ذقٔ .حذحتًنا خٛثزؼنا داربيلإا خنٔد ٙف
ٍٛدسكلأن خٛهػبفتنا دبقتشًنا جبتَا خٛهًػ ٍٛث ٌسإتنا ٙف مهخ بَٓؤث فزؼت ٙتنأ ( stress)
.ضازيلأا ِذْ زٕٚطت ٙف حذحأ خٛسبسأ خٛنآك ،دبثبٓتنلاا ٗنا خفبظلإبث ) بٓٛشلات خٛهًػٔ
( polyphenols) لُٕٛفٛنٕجهن ٍكًٚ ،دبثبٓتنلان حدبعًنأ حذسكلأن حدبعًنا بٓصئبصخن ازظَٔ
(Date seed زًتنا َٖٕ قٕحسي ٌأ ،وبًتْلان زٛثًنا ٍئ .خُيشًنا ضازيلأا ِذْ رٕطت غُي
ىٓفٔ خسارد ٗنإ فذٓت دبسارد ٘أ ذخٕت لا َّأ بًثٔ .دبجكزًنا ِذْ مثًث ُٙغ powder)
ءبعػأٔ وذنا مصي ٗهػ زًتنا َٖٕ قٕحسي ٙف ةبٓتنلاأ حذسكلأا دادبعًن مًتحًنا زٛثؤتنا
.دبَإٛحنا ٗهػ زًتنا َٖٕ قٕحسي زٛثؤت خسارذن ٖزخأ حٕطخ ْٙ خسارذنا ِذْ ٌئف ،ٌازئفنا
ٔ زًتنا َٖٕ قٕحسي ٍي لبخ ٙئاذغ وبظَ واذختسبث بػٕجسأ 35 حذًن ارؤف ٍٛؼثرأ خٚذغت ىت ذقٔ
ىث .)%2.0 ,%2.6 ,%2.4( خفهتخي تسُث زًتنا َٖٕ قٕحسي ٗهػ ٕ٘تحٚ زخا ٙئاذغ وبظَ
دبدتًُنا صبٛقٔ ،ةبٓتنلاا دادبعئ حذسكلأا دادبعي وبظُن خٕٚٛحنا دازشؤًنا صبٛق ىت
حٚزشتنا ٗنإ خفبظلإبث حشٓخلأأ وذنا ٙف حذسكلأا خٛهًػ ٍػ خدتبُنا حذسكؤًنا خُْٛذنأ خُٛٛتٔزجنا
،ءبعػلأا فئبظٔ ٙف زٛٛغت ٘أ ثذحٚ ىن زًتنا َٖٕ قٕحسي ٌأ حئبتُنا دزٓظأ ذقٔ .ٙظزًنا
طفخ ٗنإ خفبظلإبث حشٓخلأأ وذنا ٙف حذسكلأا دادبعي عبفد وبظَ ٙف ٍٛسحت ٗنإ ٖدأ َّأ بًك
ىن كنذكٔ .ءبعػلأا ِذْ ٙف حذسكلأا خٛهًػ ٍػ خدتبُنا حربعنا خُْٛذنأ خُٛٛتٔزجنا دبدتًُنا
رٔذنا ٗهػ ءٕعنا طهست حئبتُنا ِذْ ٌئف ،ّٛهػٔ .ةبٓتنلاا دبيلاػ ٗهػ زٛثؤث ٘أ ّن ٌٕكٚ
ٗنا خفبظلإبث ٘ذسكلأا زتٕتنبث خطجتزًنا خُيشًنا ضازيلأا ذظ زًتنا َٖٕ قٕحسًن ٙئبقٕنا
.دبثبٓتنلان دبعًك ّن خهًتحًنا خًْٛلأا
رازظأ ،حذسكلأا دادبعي ،خُيشًنا ضازيلأا ،زًتنا َٖٕ قٕحسي :تيسيئرلا ثحبلا ميهبفم
.لُٕٛفٛنٕجنا ،ةبٓتنلاا ،حذسكلأا
viii

Acknowledgements

I would like to thank my supervisor Dr. Carine Platat for her guidance, support, and
assistance throughout my preparation of this thesis. I am especially grateful to Dr.
Ayesha Al Dhaheri whose enthusiasm about and introduction to Environmental
science got me started.
I would like to thank the chair and all members of the Department of Biology and the
Department of Nutrition and Health at the United Arab Emirates University for
assisting me all over my studies and research. My special thanks are extended to the
Nutrition and Health Laboratories staff especially Mr. Usama Souka and Mrs. Serene
Dominic for their assistance. My thanks go to the Library Research Desk for
providing me with the relevant reference material.
Special thanks go to my parents, husband, brother, sisters and maid who helped me
along the way. I am sure they suspected it was endless. In addition, special thanks are
extended to the Al Meqbaali and Al Manei family for their assistance and friendship.
ix

Dedication

To my husband, Mohammed who has been a constant source of support and
encouragement during the challenges of graduate study and life

To my beloved mother for her constant, unconditional love and support

To the memory of my father Theyab AL Meqballi

To all of my family