Textbook of Diagnostic Microbiology, 4e

1101 Pages·2010·101.28 MB·English

by  Connie R. Mahon MS MT(ASCP) CLS

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FOURTH EDITION
Connie R. Mahon, MS
Director, Staff College
Center for Veterinary Medicine
Food and Drug Administration
Rockville, Maryland
Adjunct Faculty
Department of Clinical Laboratory Sciences
School of Medicine and Health Sciences
The George Washington University
Washington, D.C.
Donald C. Lehman, EdD, MT(ASCP), SM(NRM)
Associate Professor
Department of Medical Technology
University of Delaware
Newark, Delaware
George Manuselis, MA, MT(ASCP)
Emeritus
Medical Technology Division
The Ohio State University
Columbus, Ohio
Adjunct Faculty
Department of Natural Sciences and Forensic Science
Central Ohio Technical College
Newark, Ohio
3251 Riverport Lane
Maryland Heights, Missouri 63043
TEXTBOOK OF DIAGNOSTIC MICROBIOLOGY ISBN: 978-1-4160-6165-6
FOURTH EDITION
Copyright © 2011 by W.B. Saunders Company
All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or
transmitted, in any form or by any means, electronic, mechanical, photocopying, recording, or otherwise,
without written permission of the publisher.
Notice
Medicine is an ever-changing field. Standard safety precautions must be followed, but as new
research and clinical experience broaden our knowledge, changes in treatment and drug therapy may
become necessary or appropriate. Readers are advised to check the most current product
information provided by the manufacturer of each drug to be administered to verify the
recommended dose, the method and duration of administration, and contraindications. It is the
responsibility of the treating physician, relying on experience and knowledge of the patient, to
determine dosages and the best treatment for each individual patient. Neither the Publisher nor the
editor assume any liability for any injury and/or damage to persons or property arising from this
publication.
Permission to photocopy or reproduce solely for internal or personal use is permitted for libraries
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Previous editions copyrighted 2007, 2000, 1995
Library of Congress Cataloging-in-Publication Data
Textbook of diagnostic microbiology / [edited by] Connie R. Mahon, Donald C. Lehman, George
Manuselis.—4th ed.
p. ; cm.
Includes bibliographical references and index.
ISBN 978-1-4160-6165-6 (pbk. : alk. paper) 1. Diagnostic microbiology. I. Mahon, Connie
R. II. Lehman, Donald C. III. Manuselis, George.
[DNLM: 1. Microbiological Techniques. 2. Communicable Diseases—diagnosis. 3. Laboratory
Techniques and Procedures. QW 25 T355 2011]
QR67.T49 2011
616.9′041—dc22
2009036108
Publishing Director: Andrew Allen
Managing Editor: Ellen Wurm-Cutter
Publishing Services Manager: Patricia Tannian
Senior Project Manager: Sharon Corell
Designer: Teresa McBryan
Working together to grow
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Printed in USA www.elsevier.com | www.bookaid.org | www.sabre.org
Last digit is the print number: 9 8 7 6 5 4 3 2 1
To my husband, Dan, for his love and continued support
and understanding; my son, Sean, who inspires me; and my daughter,
Kathleen, for showing me courage.
CRM
To my wife, Terri, who has given me constant support and
encouragement and whose love makes anything I do possible;
my parents, Gerald and Sherrie, who have always been proud of me;
even though they have passed away, I know they still look over me.
DCL
To my daughters, Kristina and Shellie; my sisters, Libby and Helen;
my brother Demetrious; and in memory of my parents,
Katherine and George, for their love and encouragement.
GM
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Reviewers
Kim Boyd, AAS, BAAS, MS, MLT(ASCP), MT(AMT) Eric Landon Parnell, M(ASCP)
Assistant Professor Regional Medical Laboratories
Amarillo College Battle Creek Health Systems
Amarillo, Texas Adjunct Professor
Kellogg Community College
Russell F. Cheadle, MS, MT(ASCP) Medical Laboratory Technology Program
Associate Professor Battle Creek, Michigan
Program Director of Clinical Laboratory Science
Western Carolina University Alisa J. Petree, MHSM, MT(ASCP)
Cullowhee, North Carolina Instructor/Clinical Coordinator
Medical Laboratory Technician Program
Donna M. Duberg, MA, MS, MT(ASCP)SM, CLS(NCA) McLennan Community College
Assistant Professor Waco, Texas
Clinical Laboratory Science Department
Saint Louis University Tania Puro, EdD, CA CLS, MT(ASCP)
St. Louis, Missouri Instructor and Laboratory Coordinator
Medical Laboratory Technician and Phlebotomy Program
Rosemary Duda, MS, MT(ASCP), SM(ASCP) Hartnell College
Director, Medical Technology Program Salinas, California
St. Margaret Hospital Education Coordinator
Hammond, Indiana Clinical Laboratory Science School
Clinical Laboratory Scientist
Rita M. Heuertz, PhD, MT(ASCP) Marian Medical Center
Associate Professor Santa Maria, California
Clinical Laboratory Science Department Consultant
Internal Medicine Department Clinical Laboratory Science Program
Molecular Microbiology and Immunology Department San Francisco State University
Saint Louis University San Francisco, California
St. Louis, Missouri
Anne T. Rodgers, PhD, MT(ASCP)
Paula C. Mister, MS, MT (ASCP), SM(ASCP) Professor of Medical Technology (Retired)
Educational Coordinator and Clinical Microbiology Armstrong Atlantic State University
Laboratory Instructor Savannah, Georgia
Microbiology Department
Johns Hopkins Hospital Perry M. Scanlan, PhD, MT(ASCP)
Instructor, The Community College of Baltimore Country, Assistant Professor
Essex Campus Department of Allied Health Sciences
Baltimore, Maryland Austin Peay State University
Pathogenic Microbiology Laboratory Instructor Clarksville, Tennessee
Stevenson University, Stevenson, Maryland
Jeremy Spinney, MT(ASCP)
Hamida Nusrat, PhD Technical Manager
Lecturer, Clinical Laboratory Science Internship Program Regional Medical Laboratories, Inc.
College of Health and Human Services Adjunct Faculty
San Francisco State University Clinical Microbiology
San Francisco, California Kellogg Community College
Battle Creek, Michigan
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viii RevieweRs
Maria Lorraine Torres, EdD, MT(ASCP) K. Nisi Zell, EdD, MT(ASCP), SH, CLS(NCA)
Clinical Laboratory Science Program Director Professor (Retired)
College of Health Sciences Division of Health Sciences
University of Texas at El Paso College of Coastal Georgia
El Paso, Texas Brunswick, Georgia
Contributors
Wade K. Aldous, PhD Robert C. Fader, PhD, D(ABMM)
Lieutenant Colonel, Medical Service Corps, United States Section Chief, Microbiology/Virology Laboratory
Army Scott and White Memorial Hospital
Chief, Microbiology Assistant Professor
Department of Pathology and Area Laboratory Services Department of Pathology
Brooke Army Medical Center Texas A&M University College of Medicine
Fort Sam Houston, Texas Temple, Texas
Leona W. Ayers, MD Maribeth Laude Flaws, PhD, SM(ASCP)SI
Professor Associate Professor
Department of Pathology Rush University
School of Allied Medical Sciences Chicago, Illinois
College of Medicine
The Ohio State University Annette W. Fothergill, MA, MBA, MT(ASCP), CLS(NCA)
Columbus, Ohio Assistant Professor
Department of Pathology
Carl Brinkley, PhD Technical Director, Fungus Testing Lab
Chief, Microbiology University of Texas Health Science Center at San Antonio
U.S. Army Medical Department Center and School San Antonio, Texas
Fort Sam Houston, Texas
Olarae Giger, PhD
Maximo O. Brito, MD, FACP Lecturer, BioScience Technologies Department
Assistant Professor of Medicine Jefferson College of Health Professions
Director Thomas Jefferson University
Infectious Diseases Fellowship Training Program Main Line Health Laboratories
Section of Infectious Diseases Lankenau Hospital
Immunology and International Medicine Philadelphia, Pennsylvania
University of Illinois at Chicago
Chicago, Illinois Gerri S. Hall, PhD, D(ABMM), F(AAM)
Section Head, Clinical Microbiology
Nina M. Clark, MD Medical Director, Bacteriology and Mycobacteriology
Associate Professor Department of Clinical Pathology
University of Illinois at Chicago Director, PBL Facilitator Recruitment and Development
Director of Transplant Infectious Diseases Cleveland Clinic Lerner College of Medicine
University of Illinois Medical Center Case Western Reserve University School of Medicine
Chicago, Illinois The Cleveland Clinic
Cleveland, Ohio
James L. Cook, MD
Chief, Section of Infectious Diseases Amanda T. Harrington, PhD, D(ABMM)
Immunology and International Medicine Assistant Director, Microbiology and Molecular
Professor of Medicine, Microbiology, and Immunology Diagnostics
University of Illinois at Chicago Department of Pathology and Laboratory Medicine
Chief, Infectious Diseases VA Puget Sound Health Care System
University of Illinois Hospital Seattle, Washington
Jesse Brown Veterans Administration Hospital
Chicago, Illinois
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