Tesis Alvin TESEO

151 Pages·2011·7.77 MB·English

UNIVERSITAT POLITÈCNICA DE VALÈNCIA
Departament de Tecnologia d'Aliments

DOCTORAL THESIS

OBTENTION AND CHARACTERIZATION
OF ROSEMARY AND ASH TREE SEED
EXTRACTS AND STUDY OF THEIR
PREVENTIVE EFFECTS ON
METABOLIC DISORDERS

Presented by:
Sixto Alvin IBARRA BOBER

Directed by:
Dr. Amparo CHIRALT BOIX
Dr. Marc ROLLER

València, 2011
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DOCTORAL THESIS
OBTENTION AND CHARACTERIZATION
OF ROSEMARY AND ASH TREE SEED
EXTRACTS AND STUDY OF THEIR
PREVENTIVE EFFECTS ON
METABOLIC DISORDERS

Presented by:
Sixto Alvin IBARRA BOBER
Ingeniero Alimentario
UNIVERSIDAD FEDERICO VILLARREAL (LIMA – PERU)
Magister en Ciencia de los Alimentos
UNIVERSIDAD DE CHILE

Presented at:
UNIVERSITAT POLITÈCNICA DE VALÈNCIA

To obtain the degree:
“DOCTOR PER LA UNIVERSITAT POLITÈCNICA DE
VALÈNCIA”

Directed by:
Dr. Amparo CHIRALT BOIX
Profesora catedrática de la Universidad Politécnica de Valencia

Dr. Marc ROLLER
Scientific Director of NATUREX

València, 2011
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Acknowledgments

To

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Dedicatory

To my family, friends and colleagues

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INDEX

Page
ABBREVIATIONS - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 1

ABSTRACT - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 3
RESUMEN - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 5
RESUM - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 7
INTRODUCTION - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 9
OBJECTIVES - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 17
CHAPTER I: IMPORTANCE OF EXTRACT STANDARDIZATION AND
IN VITRO/EX VIVO ASSAY SELECTION FOR THE EVALUATION OF

ANTIOXIDANT ACTIVITY OF BOTANICALS: A CASE STUDY ON
THREE Rosmarinus officinalis L. EXTRACTS- - - - - - - - - - - - - - - - - - - - - 19
CHAPTER II: CARNOSIC ACID-RICH ROSEMARY (Rosmarinus
officinalis L.) LEAF EXTRACT LIMITS WEIGHT GAINS AND

IMPROVES CHOLESTEROL LEVELS AND GLYCAEMIA IN MICE ON A
HIGH-FAT DIET - - - - - - - - - - - - - - - - - - - - -- - - - - - - - - - - - - - - - - - - 41
CHAPTER III: IRIDOIDS FROM Fraxinus excelsior L. WITH ADIPOCYTE
DIFFERENTIATION-INHIBITORY AND PPARa ACTIVATION
61
ACTIVITY - - - - - - - - - - - - - - - - - - - -- - - - - - - - - - - - - - - - - - - - - - - - -
CHAPTER IV: Fraxinus excelsior L. SEED EXTRACT FRAXIPURE™
LIMITS WEIGHT GAINS AND HYPERGLYCEMIA IN HIGH-FAT DIET-
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INDUCED OBESE MICE - - - - - - - - - - - - - - - - - - -- - - - - - - - - - - - - - - -
CHAPTER V: ACUTE EFFECTS OF Fraxinus excelsior L. SEED
EXTRACT ON POSTPRANDIAL GLYCEMIA AND INSULIN
97
SECRETION ON HEALTHY VOLUNTEERS - - - - - - - - - - - - - - - - - - -- -
DISCUSSION - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 119
CONCLUSIONS- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 129
ANNEX - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 131

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