Terrestrial algae of the genus Klebsormidium

213 Pages·2016·7.43 MB·English

Charles University in Prague
Faculty of Science

Terrestrial algae of the genus Klebsormidium
(Streptophyta) in the light of the hypothesis
„Everything is everywhere, but the environment
selects“

Mgr. David Ryšánek
Ph.D. Thesis

Supervisor: Doc. Mgr. Pavel Škaloud, Ph.D.
Prague, 2016

Declaration

I hereby declare that I have written this thesis independently, using the listed references; or in
cooperation with other paper co-authors. I have submitted neither this thesis, nor any of its
parts, to acquire any other academic degree.

Prague, 31st May 2016
..........................................
David Ryšánek

Acknowledgements

I would like to thank Pavel Škaloud, who has been my supervisor since my bachelor study.
During this time, he had a lot of work with me and every time I needed, he gladly helped me.
Thanks to him I enjoyed this work very much.

I would like to thank all co-authors, mainly Andreas Holzinger and Fabio Rindi, for their big
help during the research and preparing manuscripts.

Thank you my colleagues from the phycology research group in Prague for friendly and
inspiring atmosphere. Special thanks go to all of you who read the previous versions of the
thesis or its summary. Your comments and ideas were really helpful.

Finally, I would like to thank my parents, because without their support it would be very
difficult to study Ph.D. and finish it.

And also I would like to thank those who are close to me for their friendship and support.

The Charles University Science Foundation provided funding of this work, project GAUK
1544214. The research stays was supported by the Czech-Austrian project AKTION 65p5.
Moreover the study was supported by the Austrian Science Fund (FWF) Project P 24242-B16
and FWF project I 1951-B16 to AH. Finally, I would like to thank Nadace "Nadání Josefa,
Marie a Zdeňky Hlávkových" for their money support at the conference in London and
Moscow.

This thesis is based on the following five papers:

1. Škaloud P., Lukešová A., Malavasi V., Ryšánek D., Hrčková K. and Rindi F. (2014)
Molecular evidence for the polyphyletic origin of low pH adaptation in the genus
Klebsormidium (Klebsormidiophyceae, Streptophyta). Plant Ecology and Evolution
147: 333-345.

2. Ryšánek D., Hrčková K. and Škaloud P. (2015) Global ubiquity and local endemism
of free-living terrestrial protists: phylogeographic assessment of the streptophyte alga
Klebsormidium. Environmental Microbiology 17: 689-698.

3. Ryšánek D., Elster J., Kováčik L. and Škaloud P. (2016) Diversity and dispersal
capacities of a terrestrial algal genus Klebsormidium (Streptophyta) in polar regions.
FEMS Microbiology Ecology 92.4: fnw039.

4. Ryšánek D., Holzinger A. and Škaloud P. (2016) Influence of substrate and pH on the
diversity of aeroterrestrial alga Klebsormidium (Klebsormidiales, Streptohyta): A
potentially important factor for sympatric speciation. Phycologia 55: 347-358.

5. Rindi F., Ryšánek D. and Škaloud P. (2016) Problems of epitypification in
morphologically simple green microalgae: a case study of two widespread species of
Klebsormidium (Klebsormidiophyceae, Streptophyta). Fottea (submitted)

Authors‟ contributions:

Paper 1. Fabio Rindi with Pavel Škaloud designed the study. Fabio Rindi, Alena Lukešová
Kristýna Hrčková and Veronica Malavasi collected and cultivated all strains. David Ryšánek
made all molecular works. Pavel Škaloud and Fabio Rindi jointly wrote the manuscript.

Paper 2. Pavel Škaloud and David Ryšánek jointly planned the study. Pavel Škaloud collected
the samples in the Czech Republic, Japan and Washington and sequenced the strains from the
Czech Republic. David Ryšánek collected samples in Wales, isolated strains from samples
from Wales, Japan, and Washington and sequenced the strains. He also performed

phylogenetic analyses together with Pavel Škaloud. Kristýna Hrčková collected and
sequenced strains from Ohio and Connecticut. David Ryšánek wrote the manuscript and Pavel
Škaloud contributed to improving the text of the manuscript.

Paper 3. David Ryšánek, Pavel Škaloud, and Josef Elster jointly planned the study. Josef
Elster and Lubomir Kovačik collected and isolated almost all strains from polar regions.
David Ryšánek collected and isolated some additional strains from Svalbard (during the 2014
expedition) and from temperate zone. He also made all molecular works and together with
Pavel Škaloud executed phylogenetic analysis. David Ryšánek wrote the manuscript and
Pavel Škaloud contributed to improving the text of the manuscript.

Paper 4. David Ryšánek, Pavel Škaloud, and Andreas Holzinger jointly planned the study.
David Ryšánek collected the strains, made molecular works and all ecophysiological
measurements. Pavel Škaloud made the statistical analysis. David Ryšánek wrote the
manuscript and Pavel Škaloud with Andreas Holzinger contributed to improving the text of
the manuscript.

Paper 5. Fabio Rindi with Pavel Škaloud designed the study. Fabio Rindi collected the
samples in Padova, Italy. Pavel Škaloud collescted samples in Strasburg, France. David
Ryšánek cultivated samples, isolated strains, made all molecular works. Pavel Škaloud and
Fabio Rindi jointly wrote the manuscript.

On behalf of all the co-authors, I declare the keynote participation (as first author) of David
Ryšánek in completing the research and writing the papers, as described above.

..........................................
Pavel Škaloud