Tandem Mass Spectrometry: Molecular Characterization

204 Pages·18.464 MB·English

by  Coelho A.V., Franco C.M.F. (Eds.)

TANDEM MASS
SPECTROMETRY

MOLECULAR CHARACTERIZATION
Edited by Ana Varela Coelho
and Catarina de Matos Ferraz Franco
Tandem Mass Spectrometry: Molecular Characterization
Edited by Ana Varela Coelho and Catarina de Matos Ferraz Franco
Contributors
Alan J Fischman, Xiao-Ming Lu, Ronald Tompkins, Diogo Ribeiro Demartini, Guilherme
Sassaki, Lauro Souza, Maria Bronze, Ana Sitoe, Francisca Lopes, Rui Moreira, Ana Varela
Coelho, Luigi Silvestro
Copyright © 2016 Second Edition
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Notice
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First published May, 2013 second - 2016
ISBN-10: 953-51-1136-1
ISBN-13: 978-953-51-1136-8

Contents
Preface VII
Section 1 General Aspects 1
Chapter 1 Matrix Effects in Mass Spectrometry Combined with Separation
Methods — Comparison HPLC, GC and Discussion on Methods
to Control these Effects 3
Luigi Silvestro, Isabela Tarcomnicu and Simona Rizea Savu
Chapter 2 A Short Overview of the Components in Mass Spectrometry
Instrumentation for Proteomics Analyses 39
Diogo Ribeiro Demartini
Section 2 Molecular Characterization by Mass Spectrometry 59
Chapter 3 Contribution of Mass Spectrometry to the Study of
Antimalarial Agents 61
Ana Raquel Sitoe, Francisca Lopes, Rui Moreira, Ana Coelho and
Maria Rosário Bronze
Chapter 4 Mass Spectrometry Strategies for Structural Analysis of
Carbohydrates and Glycoconjugates 81
Guilherme L. Sassaki and Lauro Mera de Souza
Chapter 5 Tandem MS and NMR: An Efficient Couple for the
Characterization of Saponins 117
Rita Laires, Kamila Koci, Elisabete Pires, Catarina Franco, Pedro
Lamosa and Ana V. Coelho
Chapter 6 Post-Translational Modification Profiling of Burn-Induced —
Insulin Resistance and Muscle Wasting 137
Xiao-Ming Lu, Ronald G. Tompkins and Alan J. Fischman

Preface
Tandem mass spectrometry started as a tool for the structural characterization of simple mo‐
lecular components, presently, this analytical approach is being extensively used for the
characterization and quantification of small molecules, and also of complex biomolecules.
The expeditious evolution at the instrumentation level and the increasing number of new
applications in a variety of fields indicates that a lot should still be expected from this meth‐
odology.
This book comprises six chapters divided in two sections, which provides a current review
of some general aspects in mass spectrometry and various applications of tandem mass
spectrometry in the structural characterization of a diversity of compounds, complementing
the previous books in this Tandem Mass Spectrometry series. In the first chapter are descri‐
bed several methods to overcome the matrix effects associated with mass spectrometry and
its hyphenated chromatographic methods. The second chapter provides a description of the
instrumental components relevant for proteomic applications. The second section includes
four chapters on the characterization of antimalarial agents, carbohydrates, glycoconjugates,
saponines and on the profiling of post-translational modifications.
Thanks are expressed to the contributing authors who provided their competence and lead‐
ership in the specific field attempting to provide comprehensive reviews. Finally, I would
like to thank Ms. Iva Simcic, Publishing Process Manager, and other InTech publication
staffs for their patience and professional support.
Ana Varela Coelho and Catarina de Matos Ferraz Franco
Institute of Technology, Chemistry and Biology (ITQB)
Mass Spectrometry Lab
Portugal

Section 1
General Aspects