STUDY OF COMBINING ABILITY AND HETEROSIS FOR DEVELOPMENT OF AROMATIC ...

182 Pages·2012·7.01 MB·English

STUDY OF COMBINING ABILITY AND HETEROSIS
FOR DEVELOPMENT OF AROMATIC HYBRIDS IN
RICE (Oryza sativa L.)

M. Sc. (Ag.) Thesis

by
Monalisa Behera

DEPARTMENT OF GENETICS & PLANT BREEDING
COLLEGE OF AGRICULTURE
FACULTY OF AGRICULTURE
INDIRA GANDHI KRISHI VISHWAVIDYALAYA
RAIPUR (Chhattisgarh)

2016
STUDY OF COMBINING ABILITY AND HETEROSIS
FOR DEVELOPMENT OF AROMATIC HYBRIDS IN
RICE (Oryza sativa L.)

Thesis

Submitted to the

INDIRA GANDHI KRISHI VISHWAVIDYALAYA, RAIPUR

by

Monalisa Behera

IN PARTIAL FULFILLMENT OF THE REQUIREMENTS
FOR THE DEGREE OF

Master of Science
in
Genetics & Plant Breeding

VVID No. 20141520324 ID. No. 120114106

JULY, 2016
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Scanned by CamScanner
ACKNOWLEDGMENT

“Education plays vital role in personal and social development and teacher plays
a fundamental role in imparting education. Teachers have crucial role in shaping
young people not only to face the further with confidence but also to build up it
with aim and responsibility. There is no substitute for teacher-pupil relationship”.
I take this golden opportunity to express my heartily, humble and deepest sense of
gratitude to those who helped me to complete my research possible. These words
are small acknowledgement but never fully recompensed for their great help and
co-operation.

With my sincere and deep sense of gratitude, I wish to express my sincere
gratitude to Dr. Deepak Sharma, Principal Scientist, Department of Genetics and
Plant Breeding, College of Agriculture, IGKV, Raipur and Chairman of my
Advisory Committee for his valuable guidance, planning of course work and
sustained interest in the research project.

I feel great pleasure in expressing my deep sense of gratitude and thanks to
Dr. O. N. Singh, Principal Scientist & Head, Crop Improvement Division, NRRI,
Cuttack and co-Chairman of my Advisory Committee for his keen interest and
guidance as well as constant help for the successful accomplishment of this work.

I am extremely grateful to Dr. Ramlakhan Verma, Scientist, Crop
Improvement Division, NRRI, Cuttack for providing me timely help and guidance
for successful accomplishment of this work.

I am highly obliged to Hon’ble Vice-Chancellor Dr. S.K. Patil, Dr. S.S.
Rao, Dean, College of agriculture, Dr. J.S. Urkurkar, Director Research Services
and Dr. S.S. Shaw, Director of Instructions, IGKV, Raipur for providing necessary
facilities to conduct the investigation

I am extremely grateful to Dr. A.K. Sarawgi, Professor and Head,
Department of Genetics and Plant Breeding for excellent guidance, valuable
suggestions during course of this investigation and providing necessary facilities.

I emphatically and gratefully acknowledge extend my loyal and venerable
thanks to members of my Advisory Committee, Dr. Bhawana Sharma, Assistant
Proffesor, Dr. Ritu. R. Saxena, Associate Proffesor, Department of Genetics and
Plant Breeding, Dr. Ravi. R. Saxena, Professor, Department of Agricultural
Statistics and Dr. S. B. Verulkar, Professor and Head, Department of Plant
Molecular Biology and Biotechnology for providing proper guidance and critical
comments and valuable suggestions and diligent support lead to timely completion
of this work.

I would like to express my sincere gratitude to Dr. Madhav Pandey
(Librarian, Nehru Library, IGKV, Raipur and all other members of the Nehru
Library for giving me their kind help during the study.

iii
I am short of words to express my feelings and sincere gratitude to the
biggest assets of my life, my beloved Parents, Mr. Puskar Chandra Behera & Mrs.
Saudamini Behera, younger sister Manisha Behera, all brothers and sisters for
their filial affection, obstinate sacrifice, pampered support, sincere prayers and
blessings which has been the vital source of inspiration that helped me to set
higher.

Indeed the words of my command are not adequate either in the form of
spirit, to express the depth of my humility and humbleness. Whenever, I will open
my thesis each page of this manuscript will tell the story of parents, advisors,
seniors, colleagues and juniors as well as well wishers. It will be untold strong and
can never be expressed in words.
Last but not the least; I would like to convey my cordial thanks to all those
unmentioned people who helped me directly or indirectly to fulfil my dream come
true.

Department of Genetics & Plant Breeding
College of Agriculture,
IGKV, Raipur (Chhattisgarh) Monalisa
Behera

Date:

iv
TABLE OF CONTENTS
Chapter Title Page
ACKNOWLEDGEMENT iii - iv
TABLE OF CONTENT v - vi
LIST OF TABLES vii
LIST OF FIGURES viii
LIST OF ABBREVIATIONS ix
ABSTRACT x - xiv

INTRODUCTION 1 – 4
I

REVIEW OF LITERATURE 5 - 35
II
2.1 Heterosis studies in aromatic rice 5 – 14
2.2 Combining ability analysis 14 – 26
2.3 Identification of Maintainer and Restorer lines 27 – 30
2.4 Molecular Characterization and genetic diversity 31 – 35
analysis of parents and hybrids

III 36 – 60
MATERIALS AND METHODS
3.1 Experimental Site and Layout 36 – 39
3.2 Experimental Material and methodology used 39 – 39
for the study
3.3 Methods 40 – 40
3.4 Experimental Season 40 – 41
3.5 Observation Recorded 41 – 46
3.6 Statistical Analysis 46 – 48
3.7 Combining Ability Analysis 48 - 57
3.8 Molecular Marker Analysis 57 – 58
3.9 Molecular data Analysis 59 – 60

IV 61 - 123
RESULTS AND DISCUSSION
4.1 Heterosis in Parents 61 – 74
4.2 Mean in hybrids 75 – 92
4.3. Combining Ability 93 – 112
v
4.4 Identification of restores & maintainers 113 – 114
4.5 Assessment of heterosis based on genetic diversity 114 - 123
of parents

V SUMMARY AND CONCLUSIONS 124 – 129

REFERENCES 130 – 144

APPENDICES
Appendix A 145
Appendix B 146 -148
Appendix C 149

RESUME 150

vi
LIST OF TABLES
Table Title Page
3.1 Monthly meteorological observations during crop period 37
3.2 Details of parents used in the study 39
3.3 List of characteristics taken under investigation 41
3.4 Scale for ASV scoring 46
3.5 Analysis of variance 47
3.6 ANOVA table for L x T analysis 49
3.7 Criteria for classifying the parental lines 53
3.8 Standardized concentration of PCR components 57
3.9 The schedule of temperature and duration programmed for 57
PCR amplification using SSR primers
4.1 Analysis of variance for 30 yield and quality traits in 30 64
hybrids
4.2 Mean performance for 30 yield and quality traits of 13 65 – 67
parents
4.3 Mean performance for 30 morpho-physico chemical traits of 72 – 74
30 F hybrids
1
4.4 Estimation of mid parent heterosis, heterobeltiosis and 78 – 83
economic heterosis in 30 hybrids
4.5 Analysis of variance for general combining ability (GCA) 96
and specific combining ability (SCA) for different characters
4.6 Estimate of general combining ability (GCA) effect for 97 – 99
different characters
4.7 Estimate of general combining ability (SCA) effect for 100 – 102
different characters
4.8 Rf gene status in parents and their efficacy in fertility 114
restoration
4.9 List of Identified Restorers and Maintainers 116
4.10 Efficacy of fertility restorer genes in fertility restoration in 117 – 118
WA-CMS based three line hybrids
4.11 Genetic Relationship among Ten Different Parental Lines 120
based on SSR Marker
4.12 Details of SSR markers used for characterization of CMS 122
lines

vii
LIST OF FIGURES
Figure Title Page

3.1 Monthly meteorological data during crop growth period 52

4.11 Dendrogram showing Genetic Relationship among Ten 121
Different Parental Lines based on SSR Marker

viii