"STUDIES ON CULTIVATION OF OYSTER MUSHROOM ON DIFFERENT AGRO-WASTE ...

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"STUDIES ON CULTIVATION OF OYSTER
MUSHROOM ON DIFFERENT AGRO-WASTE
SUBSTRATES IN MALWA REGION OF M.P. "
Thesis Submitted to the
Jawaharlal Nehru Krishi Vishwavidyalaya,
Jabalpur (M. P.)
in partial fulfillment of the requirements
for the Degree of
MASTER OF SCIENCE IN AGRICULTURE
(Plant Pathology )
By
ASHISH KUMAR JAIN

SIs' ,
Department of Plant Pathology
Jawaharlal Nehru Krishi Vishwavidyalaya,
College of Agriculture,
Indore (M. P.)
2001

CERTIFICATE -- I
This to certified that the thesis entitled "STUDIES ON CULTIVATION OF
OYSTER MUSHROOM (Pleurotus sajor—caju) ON DIFFERENT AGRO —
WASTE SUBSTRATE IN MALWA REGION OF M.P." submitted in partial
fulfillment of the requirements for the degree of MASTER OF SCIENCE in
AGRICULTURE (PLANT PATHOLOGY), of the Jawaharlal Nehru Krishri
Vishwa Vidyalaya, Jabalpur (M.P.) is a record of bonafide research work carried
out by SHRI ASHISH KUMAR JAIN, I. D. No. A / IN — 308/94, under my
guidance and supervision. The subject of the thesis has been approved by the
student's advisory committee and Director of Instructions.
No part of the thesis has been submitted for any other degree or diploma
(Certificate awarded etc.) or has been published / published part has been fully
acknowledged. All the assistance and help received during the course of the
investigations have been duly acknowledged by him.
Place: INDORE Dr. . L. NAIK
Date: Q1"v C, ~, , i (Chairman of the Advisory Committee)
THESIS APPROVED BY THE STUDENT'S ADVISORY COMMITTEE
Chairman. - Dr. S. L. Naik ---- ----------
Member - Dr. N. K. Jain ----- - ----
Member - Dr. K. N. Kapoor ----
Member - Dr. S. C. Pandya ----- ~ -`--`---
CERTIFICATE —II
0
This to certified that the thesis entitled `STUDIES ON
CULTIVATION OF OYSTER MUSHROOM (P1eurotus sajor — caju) ON
DIFFERENT AGRO-WASTE SUBSTRATE IN MALWA REGION OF
M.P." submitted, by SHRI ASHISH KUMAR JAIN, 1. U. No. A/ IN — 308/94,
to the Jawaharlal Nehru Krishri Vishwa Vidyalaya, Jabalpur (M.P.) in partial
fulfillment of the requirements for the degree of MASTER OF SCIENCE in
AGRICULTURE, in the department of Plant Pathology, has been approved by
the student's advisory committee after an oral examination on the same.

Place : INDORE Dr. S. CNAIK

Date : C/ .'. 2c't) / (Chairman of the Advisory Committee)
MEMBERS OF TIIE STUDENT'S ADVISORY COMMITTEE
Chairman Dr. S. L. Nark
Member - Dr. N. K. Jain
Member - Dr. K_ N. Kapoor
Member - Dr. S. C. Pandya
READ OF TIDE SECTION -
DEAN _
ofAtIcidt.r4 th$L Mi;
D#rectoro .In tnvXf x +
JNKishi VishwiVidyfliy*
,A,BALPUR (M.P.)
ACKNOWLEDGEMENT
I feel extremely indebted to Dr. S. L. Naik, Associate professor and
Head, Plant Pathology section, College of Agriculture, Indore and chairman of
my advisory committee for valuable guidance, constant encouragement and
sustained interest in the present investigation and constructive criticism during
the preparation of the manuscript.
I earnestly express the sense of gratitude to members of my advisory
committee, Dr. N. K. Jain Associate Professor (Plant Pathology), Dr. K. N.
Kapoor Assoc. Professor (Entomology) and Dr. S. C. Pandya, Assoc. Professor,
(statistics) for their unrestricted help, encouragement and co-operation through
out the course of research.
With profound sense of respect, I wish to express my sincere regards
to Dr. 0. P. Verma, Dean, College of Agriculture, Indore for his constant help
and providing necessary facilities for carrying our the research work.
I express my gratitude to Professors, my teachers Dr. A. Krishna, scientist
(Plant Pathology) and Shri S. M. Matkar Assit. Professor (Entomology) for their
encouragement, kind co-operation and valuable suggestions during the
preparation of the manuscript.
I am thankful to Dr. U. S. Thakur, Librarian and entire library staff to
permit me to avail the library facilities.
I express my sincere thanks to Messrs. V. Agrawal, B. Patidar, K. S.
Baghel, M. Dwivedi, D. Paliwal, Amit Dwivedi, Atul Patel, Anjum Qureshi, A.
Garewal, Ashish Jain, R. Jain, A. Parganiha, Ghansu, R. Raghuvansi, Jitu bhaiya,
Prabhat, Praveen and all my friends for their co-operation and kind support.
I am also thankful to Shri J. N. Jamie, M. H. Khan and Shyam Sunder
Mishra of the Dept. Plant Pathology for their assistance during the period of
investigation.
At last but not the least no words fail to express heartiest feeling of my
humble gratitude, profound sense of appreciation to my respected pappa Shri
Surendra Kumar Jain and mummy Smt. Shashi Prabha, Tao Prakash Chandra
Jain, Tai Kala Bai and younger brother Manish and Sachin whose love, blessing,
devotion, constant inspiration and care throughout my life enabled me to achieve
this seemingly invincible goal, made my education possible and brought me to
present level.
Place : hndore

Date: P9jii jao©l ASHISH KUMAR JAIN
CONTENTS
CHAPTER NO. TITLE PAGE NO.
I. Introduction 1-4
II. Review of literature 05-21
III . Materials and methods 22-29
IV. Experimental findings 30-41
V. Discussion 42-45
VI. Summary, conclusion and suggestions 46-47
Bibliography
48-56
Appendices
Vita
Table No. Title Page No.
4.1 Duration cost and net profit for spawn production by 31
P. sajor-caju on different food grains.
4.2 Effect of different substrates on average fresh yield 33
(g / 500g of dry substrate) performance of P.sajor-caju.
4.3.1 Fresh yield and biological efficiency of oyster mushroom 35
on different substrate combinations.
3.2.2 A comparison of mushroom yield by enrichment of cotton
and groundnut with other substrate. 36
4.4 Fresh yield of oyster mushroom (P. sajor-caju) by supple-
mention of spent up straw with the substrate. 37
4.5 Effect of different pasteurization methods on fresh yield
of oyster mushroom (P. sajor-caju). 39
4.6 Relation of production technology and yield (g / 500 g of
dry substrate) of oyster mushroom :for best substrate /
substrate combination. 41
LIST OF PLATES
Plate-1
Culture of Pleurotus sajor-caju in Petri plate and test tube.
Spawn preparation of Pleurotus sajor-caju on various grains viz, wheat, jowar,
kodo, kutki and baira in bottles.
Spawn preparation of Pleurotus sajor-caju on wheat in polythene bag and flask.
Plate-2
Different substrate for oyster mushroom cultivation.
Spawn run in polythene bag.
Plate-3
Growth of oyster mushroom in cotton, groundnut and wheat substrates.
Plate-4
Growth of oyster mushroom in cotton + wheat and groundnut + wheat substrates.
List of figures
Figure
No. Title .. Page No•
1. Duration, cost and net profit for spawn production by P. sajor-caju 31
on different food grains.
2. Effect of different substrate on fresh yield of mushroom. 33
3. Fresh yield of Oyster mushroom by supplementation. 37
4. Effect of different pasteurization methodson fresh yield. 39
5. Relation of production technology and yield. 41