Table Of ContentM M B ™
ETHODS IN OLECULAR IOLOGY
Series Editor
John M. Walker
School of Life Sciences
University of Hertfordshire
Hat fi eld, Hertfordshire, AL10 9AB, UK
For further volumes:
http://www.springer.com/series/7651
wwwwwwwwwwww
Stem Cell Mobilization
Methods and Protocols
Edited by
Mikhail G. Kolonin and Paul J. Simmons
Institute of Molecular Medicine, University of Texas Health Science Center at Houston, Houston, TX, USA
Editors
Mikhail G. Kolonin Paul J. Simmons
Institute of Molecular Medicine Institute of Molecular Medicine
University of Texas Health Science University of Texas Health Science
Center at Houston Center at Houston
Houston, TX, USA Houston, TX, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic)
ISBN 978-1-61779-942-6 ISBN 978-1-61779-943-3 (eBook)
DOI 10.1007/978-1-61779-943-3
Springer New York Heidelberg Dordrecht London
Library of Congress Control Number: 2012943077
© Springer Science+Business Media, LLC 2012
This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is
concerned, specifi cally the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction
on microfi lms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation,
computer software, or by similar or dissimilar methodology now known or hereafter developed. Exempted from this
legal reservation are brief excerpts in connection with reviews or scholarly analysis or material supplied specifi cally for
the purpose of being entered and executed on a computer system, for exclusive use by the purchaser of the work.
Duplication of this publication or parts thereof is permitted only under the provisions of the Copyright Law of the
Publisher’s location, in its current version, and permission for use must always be obtained from Springer. Permissions
for use may be obtained through RightsLink at the Copyright Clearance Center. Violations are liable to prosecution
under the respective Copyright Law.
The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not
imply, even in the absence of a specifi c statement, that such names are exempt from the relevant protective laws and
regulations and therefore free for general use.
While the advice and information in this book are believed to be true and accurate at the date of publication, neither
the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be
made. The publisher makes no warranty, express or implied, with respect to the material contained herein.
Printed on acid-free paper
Humana Press is a brand of Springer
Springer is part of Springer Science+Business Media (www.springer.com)
Preface
Initially noted as a phenomenon accompanying the recovery of patients from myelosup-
pressive chemotherapy, stem cell mobilization represents a transient increase in the levels of
circulating stem and progenitor cells. Observation of progenitor cell mobilization follow-
ing the administration of certain factors, such as G-CSF, heralded a new era in hematologi-
cal cell therapies with “mobilized blood” now essentially replacing bone marrow as the
tissue of choice for hematopoietic reconstitution in cancer therapy. There is considerable
interest in the phenomenon of mobilization in terms of understanding the underlying
molecular mechanisms that drive the process of cell egress from the bone marrow. Recent
studies have also revealed mobilization of progenitor cells from organs other than the bone
marrow, although the importance of this phenomenon and the extent to which extramed-
ullary cells contribute to the mobilized pool are yet to be understood. The notion that
progenitor cell mobilization results in systemic redistribution of several cell populations
that may participate in repair and regeneration has considerable clinical implications. While
recruitment of systemically circulating stem cells may be benefi cial in bone marrow recon-
stitution or wound healing settings, progenitor traffi cking to lesions in cancer or other
fi brotic conditions could have adverse effects. Therefore, development of reliable methods
to quantify traffi cking and read out activity of individual precursor cell types is highly impor-
tant. This book aims to overview the current standing in cell mobilization methodology
and to outline recent developments in the fi eld for basic and biomedical research community.
Speci fi cally, clinical hematopoietic progenitor cell mobilization protocols and the
experimental techniques used in animal models are covered in Chapters 1 – 11 . The remaining
part of the book addresses the frontiers in mobilization and analysis of non-hematopoietic
progenitors, with specifi c emphases on endothelial progenitor cells (Chapters 1 2 – 14 ) ,
mesenchymal progenitor cells (Chapters 15 and 2 0 ), monocyte-derived fi broblast progeni-
tors (Chapter 1 6 ), and very small embryonic-like cells (Chapter 1 7 ). Advanced methodologies
to analyze physiological and pathological functions of these distinct progenitor populations
are also described (Chapters 1 4 – 15 , 18 – 19 ).
Houston, TX , USA Mikhail G. Kolonin
Paul J. Simmons
v
wwwwwwwwwwww
Contents
Preface ..................................................................................................................... v
Contributors ............................................................................................................. ix
1 Mobilization of Hematopoietic Stem/Progenitor Cells:
General Principles and Molecular Mechanisms ................................................... 1
Halvard Bonig and Thalia Papayannopoulou
2 Quantifying Hematopoietic Stem and Progenitor Cell Mobilization .................. 15
Shiri Gur-Cohen, Kfir Lapid, and Tsvee Lapidot
3 Hematopoietic Stem Cell Mobilization with G-CSF .......................................... 37
Chitra Hosing
4 Hematopoietic Stem Cell Mobilization with Agents Other than G-CSF ............. 49
Jonathan Hoggatt and Louis M. Pelus
5 Hematopoietic Stem Cell Mobilization: A Clinical Protocol .............................. 69
Gina Pesek and Michele Cottler-Fox
6 Monitoring Blood for CD34+ Cells to Determine Timing
of Hematopoietic Progenitor Cells Apheresis..................................................... 79
M. Louette Vaughn and Edmund K. Waller
7 Hematopoietic Progenitor Cell Collection ........................................................ 85
S. Darlene Marlow and Myra House
8 Managing Apheresis Complications During the Hematopoietic
Stem Cell Collection ......................................................................................... 93
S. Darlene Marlow and Myra House
9 Hematopoietic Progenitor Cell Apheresis Processing ......................................... 97
Eleanor S. Hamilton and Edmund K. Waller
10 Toxicities of Mobilized Stem Cell Infusion ........................................................ 111
Jonathan L. Kaufman
11 Mobilization of Hematopoietic Stem Cells by Depleting Bone
Marrow Macrophages ....................................................................................... 117
Valérie Barbier, Ingrid G. Winkler, and Jean-Pierre Lévesque
12 Combinatorial Stem Cell Mobilization in Animal Models .................................. 139
Simon C. Pitchford and Sara M. Rankin
13 Vascular Progenitor Cell Mobilization ............................................................... 155
Kirsten A. Kienstra and Karen K. Hirschi
14 Evaluation of Circulating Endothelial Precursor Cells in Cancer Patients ........... 165
Francesco Bertolini, Patrizia Mancuso, Liat Benayoun,
Svetlana Gingis-Velitski, and Yuval Shaked
vii
viii Contents
15 Tracking Inflammation-Induced Mobilization of Mesenchymal Stem Cells ........ 173
Erika L. Spaeth, Shannon Kidd, and Frank C. Marini
16 Differentiation of Circulating Monocytes into Fibroblast-Like Cells .................. 191
Darrell Pilling and Richard H. Gomer
17 Enumeration of Very Small Embryonic-Like Stem Cells in Peripheral Blood ...... 207
Rui Liu and Mariusz Z. Ratajczak
18 Generation of a Vascular Niche for Studying Stem Cell Homeostasis ................. 221
Jason M. Butler and Shahin Rafii
19 Studying Vascular Progenitor Cells in a Neonatal Mouse Model ........................ 235
Kirsten A. Kienstra and Karen K. Hirschi
20 Progenitor Cell Mobilization from Extramedullary Organs ................................ 243
Mikhail G. Kolonin
Index ................................................................................................................................ 253
Contributors
VALÉRIE BARBIER • Mater Medical Research Institute, Aubigny Place, Raymond Terrace,
South Brisbane, QLD , Australia
LIAT BENAYOUN • Department of Molecular Pharmacology , Technion–Israel Institute of
Technology, Haifa , Israel
FRANCESCO BERTOLINI • Laboratory of Hematology-Oncology , European Institute
of Oncology, Milan , Italy
HALVARD BONIG • Department of Medicine/Hematology , University of Washington, Seattle ,
WA , USA
JASON M. BUTLER • Weill Cornell Medical College and the Howard Hughes Medical
Institute, Cornell University, New York , NY , USA
SHIRI GUR-COHEN • Department of Immunology, Weizmann Institute of Science,
Rehovot , Israel
MICHELE COTTLER-FOX • Department of Pathology, University of Arkansas for Medical
Sciences, Little Rock, AR , USA
SVETLANA GINGIS-VELITSKI • Department of Molecular Pharmacology , Rappaport Faculty of
Medicine, Technion–Israel Institute of Technology, Haifa , Israel
RICHARD H. GOMER • Texas A&M University, College Station, TX , USA
ELEANOR S. HAMILTON • Cellular Therapies Laboratory , Emory University Hospital,
Atlanta , GA , USA
KAREN K. HIRSCHI • Division of Neonatology, Department of Pediatrics, Baylor College
of Medicine , Houston, TX , USA
JONATHAN HOGGATT • Department of Microbiology and Immunology, Indiana University
School of Medicine, Indianapolis, IN , USA
CHITRA HOSING • Department of Stem Cell Transplantation and Cell Therapy,
M.D. Anderson Cancer Center, Houston, TX , USA
MYRA HOUSE • Center for Transfusion and Cellular Therapies (CTCT), Emory University
Hospital , Atlanta , GA , USA
JONATHAN L. K AUFMAN • Hematology and Medical Oncology , Winship Cancer Institute,
Emory University School of Medicine, Atlanta , GA , USA
SHANNON KIDD • Department of Leukemia, The University of Texas MD Anderson Cancer
Center , Houston, TX , USA
KIRSTEN A. KIENSTRA • Division of Neonatology, Department of Pediatrics, Baylor College
of Medicine, Houston, TX , USA
MIKHAIL G. KOLONIN • Institute of Molecular Medicine, University of Texas Health Science
Center at Houston, Houston, TX , USA
KF I R LAPID • Department of Immunology, Weizmann Institute of Science, Rehovot , Israel
TSVEE LAPIDOT • Department of Immunology, Weizmann Institute of Science, Rehovot , Israel
JEAN-PIERRE LÉVESQUE • Mater Medical Research Institute, South Brisbane, QLD ,
Australia
RUI LIU • Developmental Biology Program, James Graham Brown Cancer Center, University
of Louisville Louisville, KY USA
ix
x Contributors
PATRIZIA MANCUSO • Laboratory of Hematology-Oncology , European Institute of Oncology,
Milan , Italy
FRANK C. MARINI • Institute for Regenerative Medicine, Comprehensive Cancer Center ,
Wake Forest University, Medical Center Blvd. Winston-Salem, NC
S. DARLENE MARLOW • Center for Transfusion and Cellular Therapies, Emory University
Hospital , Atlanta , GA , USA
THALIA PAPAYANNOPOULOU • Department of Medicine/Hematology , University
of Washington, Seattle , WA , USA
LOUIS M. PELUS • Department of Microbiology and Immunology, Indiana University
School of Medicine, Indianapolis, IN , USA
GINA PESEK • Department of Pathology, University of Arkansas for Medical Sciences,
Little Rock, AR , USA
DARRELL PILLING • Texas A&M University, College Station, TX , USA
SIMON C. PITCHFORD • Leukocyte Biology Section, Faculty of Medicine National Heart
and Lung Institute, Imperial College London, London, UK
SHAHIN RA FI I • Weill Cornell Medical College and the Howard Hughes Medical
Institute, Cornell University, New York , NY , USA
SARA M. RANKIN • Leukocyte Biology Section, Faculty of Medicine National Heart and
Lung Institute, Imperial College London, London, UK
MARIUSZ Z. RATAJCZAK • Developmental Biology Program, James Graham Brown Cancer
Center , University of Louisville, Louisville, KY , USA
YUVAL SHAKED • Department of Molecular Pharmacology , Technion-Israel Institute of
Technology, Haifa , Israel
PAUL J. SIMMONS • Institute of Molecular Medicine, University of Texas Health Science
Center at Houston, Houston, TX , USA
ERIKA L. SPAETH • Department of Leukemia, The University of Texas MD Anderson Cancer
Center , Houston, TX , USA
M. LOUETTE VAUGHN • Center for Transfusion and Cellular Therapies, Emory University
Hospital , Atlanta , GA , USA
EDMUND K. WALLER • Cellular Therapies Laboratory, Emory University Hospital, Atlanta,
GA, USA
INGRID G. WINKLER • Mater Medical Research Institute, Aubigny Place, Raymond Terrace,
South Brisbane, QLD , Australia
