Table Of ContentSpectroscopy of Biological Molecules: New Directions
SPECTROSCOPY OF
BIOLOGICAL MOLECULES:
NEW DIRECTIONS
8th European Conference on the Spectroscopy of
Biological Molecules, 29 August-2 September 1999,
Enschede, The Netherlands
Edited by
J. GREVE
University of Twente,
Enschede, The Netherlands
G. J. PUPPELS
Erasmus University,
Rotterdam, The Netherlands
and
C.OTTO
University of Twente,
Enschede, The Netherlands
KLUWER ACADEMIC PUBLISHERS
DORDRECHT I BOSTON I LONDON
A C.I.P. Catalogue record for this book is available from the Library of Congress.
ISBN 0-7923-5847-3
Published by Kluwer Academic Publishers,
P.O. Box 17,3300 AA Dordrecht, The Netherlands.
Sold and distributed in North, Central and South America
by Kluwer Academic Publishers,
101 Philip Drive, Norwell, MA 02061, U.S.A.
In all other countries, sold and distributed
by Kluwer Academic Publishers,
P.O. Box 322, 3300 AH Dordrecht, The Netherlands.
Printed on acid-free paper
All Rights Reserved
© 1999 Kluwer Academic Publishers
No part of the material protected by this copyright notice may be reproduced or
utilized in any form or by any means, electronic or mechanical,
including photocopying, recording or by any information storage and
retrieval system, without written permission from the copyright owner.
TABLE OF CONTENTS
Preface xxxiii
Section I: Proteins and Peptides
Protein structure-function relationship studied by single crystal polarized
absorption microspectrophotometry 3
A. MOZZARELLIandS. BETIATI
New approaches to reaction-induced FTIR difference spectroscopy of proteins 7
D.A.Moss
Nanosecond UV resonance Raman examination of initial steps in a.-helix
secondary structure evolution 11
I.K. LEDNEV, A.S. KARNOUP, M.C. SPARROW and S.A. AsHER
Function-related conformational changes of protein molecules revealed by
Raman spectroscopy 13
A.Yu. CHIKlSHEV, C. OTIO, N.N. BRANDT, V.V. MOLOOOZHENYA, LK.
SAKODYNSKAVA, J. GREVEandN.I. KOROTEEV
Physical studies of Cold Denaturation of Beta-lactoglobulin 15
J. BOHR and H. BoHR
Characterization of the binding of endogenous proteins to endotoxins: binding
stochiometry and protein secondary structure 17
K. BRANDENBURG and G. LEHWARK-YVETOT
Alteration of the internal dynamics of Escherichia coli alkaline phosphatase
under proteolytic modification, thermal inactivation and refolding 19
V.M. MAzHuL and S.G. KANANOVICH
Room Temperature Tryptophan Phosphorescence as monitor of internal
dynamics of isolated human erythrocyte membranes proteins 21
V.M. MAzHuL, D.G. SHCHARBIN, LV. IVIN, E.M. ZAITSEVA and S;V. DRIAMOV
Reactivity of peroxo a.-amino acid complexes of molybdenum (VI) towards
nitric oxide and carbon oxide in water solutions 23
R. HASSA and J.~. DZIEGIELEWSKI
Thermal unfolding of tendamistat probed by Fourier-transform infrared
spectroscopy 25
C. ZSCHERP, H. AVGON, C. ERND, J.W. ENGELS and W. MANTELE
Spectrophotometric confirmation for oxygen ligation to the reduced haem d of
1
Pseudomonas nautica nitrite reductase 27
S. BESSON, G. FAUQUE, I.J.G. MOURAandL MOURA
v
vi
Effect of temperature upon luminescence of thyreoglobulin 29
N. NIZOMOV, M. YANGIBAYEV, G. HODJAEV andF. HAYDAROVA
Film formation from wheat gluten proteins by FTIR spectroscopy 31
C. MANGAVEL, A.C. SANCHEZ, Y. POPINEAU and J. GUEGUEN
FTIR difference spectroscopy of protein-ligand interactions 33
K. FOCHSLE and D.A. Moss
Structure and dynamics of globin hydrolyzates gel formation 35
X. LIU, M. NAKAJIMA and Y. SANO
Photophysics of P-pyridoxyl amino acids. Time-resolved fluorescence study
and molecular modelling 37
V. STEPURO and S. MASKEVICH
Interaction of glycosylated amino acids with human serum albumin 39
I.I. STEPUROandN.A. CHAIKOVSKAYA
Biomolecular interactions studied by FT-IR-ATR spectroscopy 41
M. BOESE and K. FAHMY
Vibrational spectra of the cyclic dipeptides (L-ASP-L-ASP) and (L-GLU-L
GLU) 43
A.P. MENDHAM, B.Z. CHOWDHRY, MJ. SNOWDEN and R. WITHNALL
Investigation of induced by low temperature water-soluble proteins
conformational rearrangements 45
T.S. DYUBKO, E.A. ROMODANOVA, V.A. GAvRIKandYu.G. OKLADNOY
Raman and CD spectroscopy of the interaction between tRNA and
glyceraldehyde-3-phosphate dehydrogenase 47
P. CARMONA, A. RODRiGUEZ-CASADO and M. MOLINA
Fluorescence and circular dichroism studies of plasminogen activator inhibitor
I (PAl-I) and its interactions with the environment and with ligands 49
D.J. COWLEY and B. HEINTZELMANN
A spectroscopic study of various conformational states of poly(j3-benzyl, L
aspartate) 51
P. TANDON, D. KApOOR, N.K. MISRA and V.D. GUPTA
On Independent Denaturation of Albumin and y-Globulins in Composition of
Human Blood Serum 53
J. MONASELIDZE and D. KHACHlDzE
vii
Thermodynamic Origin of Cisffrans Isomers of N-substituted Amides and a
Proline-Containing ~turn Model Dipeptide in Aqueous Solution: A Variabl~
Temperature Gradient Enhanced lH_NMR Study 55
z.
ATIIANASSIOU, A. TROOANIS and I.P. OEROTHANASSIS
Protein adsorption on soil mineral surfaces 57
s.
SERVAGENT-NOINVILLB, M. REVAULT, H. QUIQUAMPOlxandM-H BARON
Effect of urea on Ca2+-ATPase conformation and activity studied by infrared
spectroscopy 59
I. ILoRO, A. PRADO and I.L.R. ARRONDO
Protein hydrogen-deuterium exchange Ff-IR spectroscopy and secondary
structure correlation 61
B.I. BAELLO, P. P ANCOSKA and T.A. KEIDERLING
Vibrational circular dichroism with isotopic substitution. Application to alanine
rich peptides 63
T.A. KEIDERLlNG, R.A.O.D. SILVA, S.M. DECATUR and P. BOUR
Intermediates in the thermal unfolding of nbonuclease A 65
S. STELEA, P. PANCOSKA and T.A. KEIDERLING
Protein spectra-structure relationship by novel modification of 2D correlation
analysis. VCD applications 67
I. KUBELKA, P. PANCOSKA and T.A. KEIDERLING
Effect of the alkyl chain modifications of biotinyl derivatives to the binding
with streptavidin 69
A. TORREGGIANI, S. BONORA and O. FINI
Copper(ll) complex with L-carnosine as a ligand: the tautomeric change of the
imidazole moiety upon complexation 71
A. TORREGGIANI, M. TAMBA and o. FINI
Oxidized-minus-reduced FfIR difference spectroscopy of cytochrome c crystals 73
D.A. MossandJ.T. SAGE
Characterization of natural and site-directed mutations in redox proteins with
FfIR spectroelectrochemistry 75
A. WOLF andD.A. Moss
Confonnational analysis and vibrational frequencies of alanine and its sulphur
tautomers 77
L.A. BATISTA DE CARVALHO, M.P.M. MARQUES and I.I.C. TEIXEIRA-DIAS
Reversible dioxygen binding by the sublimated films of some meso
tetraarylpOIphyrinatocobalt{IT) complexes 79
T.S. KURTIKYAN, 0.0. MARTIROSYAN and V.N. MAnAKYAN
viii
Spectroscopic characterization of Bombyx morl silk fibroin: Raman spectrum of
Silk I 81
P. MONTI, G. FREDDI, M. TSUKADA, A. BERTOLUZZA and T. AsAKURA
Photophysics and high-resolution spectroscopy of green fluorescent protein 83
T.M.H. CREEMERS, A.J. LocK, V. SUBRAMANIAM, T.M. JOVIN and S. VOLKER
FTIR and 2D-FTIR spectroscopic study of cytochrome Cm adsorbed on anionic
and hydrophobic surfaces . 85
S. LECOMTE, C. HILLERITEAU, J.P. FORGERIT, M. REVAULT, M.H. BARON, P.
HILDEBRANDT and T. SOULIMANE
Functional topology of the bacterial RNA polymerase. Multipoint monitoring
by a fluorescent probe 87
O.N. OZOLINE, N. FumA andA. IsHIHAMA
Individual green fluorescent proteins (GFP) studied by near-field optical
microscopy 89
M.F. GARCIA-PARAJO, J.-A. VEERMAN, G.M.J. SEGERS-NoLTEN, B.G. DE
GROOTH, J. GREVEandN.F. VAN HULST
Section II: Chromophores and chromophoric proteins
Resonance Raman Dispersion Spectroscopy probes asymmetric distortions of
porphyrins in solution and proteins 95
R. SCHWEITZER-STENNER
Biological photosensors studied by laser-induced optoacoustic spectroscopy 99
S.E. BRASLA VSKY
em
The electron transfer dynamics of cytochrome from Thermus thermophilus
probed by time-resolved surface enhanced resonance Raman spectroscopy 103
S. LECOMTE, P. HILDEBRANDT and T. SOULIMANE
The mechanism of the electron transfer process from cytochrome c to
cytochrome c oxidase studied by resonance Raman spectroscopic techniques 107
P. HILDEBRANDT, S. DOPNER, H. WACKERBARTH, F.I. ROSELL, A. GRANT
MAUl{, G. BUSE and T. SOULIMANE
Fluorescence Microscopy on Single Light Harvesting Complexes 111
C. DETZ, A. DRABENSTEDT, 1. SCHUSTER and J. WRACHTRUP
Exciton lineshapes ofLH2 photosynthetic antenna proteins 115
A. FREIBERG, R. Ruus and K. TlMPMANN
ix
Time-resolved spectroscopy ofluciferin 119
E.Yu. CHEREDNIKOVA, A.Yu. CHooSHEV, M. MIZUNO, M. SAKAI and H.
TAKAHASHI
Spectroscopic properties of the new metal-porphyrins 121
Y.V. KOROVIN, M.Yu. RUSAKOVA, A.V. PopovAandZ.I. ZHiLINA
Complexes formed between porphyrin boWld to copolymer and transition metal
ions and analogous simple systems in water-organic solutions 123
A.V. UDAL'TSOV, K.N. nMOFEEV and A.A. CHURIN
The cytochrome oxidases from P. denitrificans, T. thermophilus, E. coli and
bovine heart studied by electrochemistry and FTIRlUVNIS spectroscopy 125
P. HELLWIG, T. SOULIMANE, U. PFITZNER, J. BEHR, F. THOMSON, B. LUOWlG, R.
GENNIS, H. MICHEL, G. BUSE and W. MANTELE
Spectral-Kinetic Studies of Excitation Relaxation Processes in Multimolecular
Porphyrinic Arrays 127
CH. VON BORCZYSKOWSKI, U. REMpEL, A. WILLERT, A.M. SHULGA, S.M.
BAcmLO and E.I. ZENKEVICH
Influence of Steric Effects on Spectral-Kinetic Properties of Meso-Nitrophenyl
Porphyrins and Their Dimers at 295 K 129
E.I. ZENKEVICH, V.N. KNYuKsHTO, A.M. SHULGA, E.I. SAGUN and S.M.
BACHILO
Phosphorescent analysis of muscle glycogen phosphorylase b internal dynamics 131
V.M. MAzHuL, E.M. ZAITSEVA, L.G. MITSKEVICH, N.V. FEDURKINA and B.I.
KURGANOV
Luminescence Properties of 8-azasteroid Molecules 133
A.A. AKHREM, N.A. BORISEVICH, O.V. GULYAKEVlCH, A.L. MiKHALCHUK, S.A.
TiKHOMIROV and G.B. TOLSTOROZHEV
Direct determination of the photochemically active electronic state by optical
spectroscopy methods 135
I.V. STANISHEVSKY and K.N. SOLOVYOV
Mossbauer effect study of modified human hemoglobin 137
M.I. OSHTRAKH, O.B. MILDER, V.A. SEMIONKIN, A.L. BERKOVSKY, M.A.
AzHIGIROVAandE.P. VYAZOVA
Vibrational study, of the 2',6'-dimethoxyflavone.cis-HCOOH inclusion
compoWld 139
L. VRiEL YNCK, J.C. MERLIN and J.C. WALLET
Quantum chemical modelling and spectroscopic studies of the protein
astaxanthin interactions in a-crustacyanin 141
R.J. WEESIE, J.C. MERLIN, H.J.M. DE GROOT, G. BRiTION, J. LUGTENBURG,
F.J.H.M. JANSEN and J.P. CoRNARO
x
Class III heme-containing peroxidases: evidence for a quantum-admixed spin
state 143
A. FEIS, B.D. HOWES, C.INDIANI, M.P. MARZOCCHI and G. SMULEVICH
Calcium depletion of horseradish peroxidase generates a quantum mechanical
mixed-spin heme state 145
C. INDIANI, B.D. HOWES, A. FEIS and G. SMULEVICH
The photolysis of carbonyl horseradish peroxidase studied by laser-induced
optoacoustic spectroscopy 147
A. FEIS and L. ANGELONI
Role of the distal phenylalanine 41 on the properties of horseradish peroxidase
C 149
H.A. HEERING, G. SMULEVICH andA.T. SMITH
Vis- and CD-spectroscopic studies of the interaction of amphiphiles with
optically active J-aggregates 151
A. QUART, H. VON BERLEPSCH, C. BOETTCHER, S. KiRSTEIN and S. DAEHNE
Aerobic photoinhibition treatment leads to formation of QAH2 and efficient
charge separation in PS II reaction centers 153
K. GIBASIEWICZ
Spectroscopic studies of rhodopsin mutants 155
A. Kosoy, A. ANDREs, E. ROCA, P. GARRIGA and J. MANYOSA
Resonance Raman spectroscopy reveales different conformers of Ni(II)- and
Cu(II)-octamethylchlorin in CS2-so1ution 157
R.J. LIPSKI and R. SCHWEITZER-STENNER
Solvatochromism gives a clue to the locations of the S2+-S0 and Tn+-TJ peaks
ofLHCII xanthophylls 159
T. JA VORFI and K. RAzI NAQVI
Steric interactions, manifested in vacuum ultraviolet spectral region for thin
films of tryptophan-containing small peptides 161
N.E. QVCHINNIKOVA and N.M. TSYGANENKO
Luminescence revealed by chain phototransformation of aromatic amino acids
in aqueous solution 163
V.M. BAKULEV and V.M. PEREIRA
Electrochromism of carotenoids in vitro - conformational modulation of
molecular polarizability 165
S. KRAWCZYK
xi
NMR spectroscopic methodologies for investigating porphyrin ruffling in
hemoproteins and model compounds 167
C.G. KALODIMOS and I.P. GEROTHANASSIS
Why does the primary electron donor in photosynthetic reaction centers have
dimeric structure? 169
V.V. NECHAEV, 0.0. TATARENKO andM.V. STROBKOVA
Vibrational analysis of model compounds for the tetrapyrrole chromophore in
phytochrome 171
M.A. MROOINSKI, C.O. DELLA VtJOOVA, K. NEMETH, C. KNEIP, P.
ffiLDEBRANDTandF.MARK
Photophysics of NH tautomers of 2-N0 -5,IO,15,20-tetraphenylporphyrin in
2
solvents of different polarity 173
V.S. CHiRVONY, S.M. BACHlW, S.l SHiSHPORENOK, I.V. FILATOV, lV.
AVIWV, S.N. TEREKHov, P.P. PERSHUKEVICH, A. VAN HOEK, T.1. SCHAAFSMA
and V.L. MALINOVSKII
The correlation of stretch vibrations frequencies with bond dissociation
energies. Application to carotenoids 175
E.I. FINKELSHTEIN
NADH binds in the mitochondrial respiratory chain far from flavins of
flavoproteins 177
V.I. SUKHAREV and N.L. VEKSHIN
Optical spectroscopy and simulation-based analysis of thin films of zinc
porphyrin derivatives 179
M.M. YATSKOU, T.1. SCHAAFSMA, V.V. A1'ANASOVICH, R.B.M. KOEHORST, A.
VAN HOEK and E.G. NoVIKov
Time-resolved nonlinear polarization-modulated spectroscopy of
bacteriorhodopsin 181
O.A. KULIKOVSKA, V.B. TARANENKO and V.Yu. BAZHENOV
Artificial receptors: molecular modelling studies of calix[4]resorcinarene
capped porphyrins 183
M. BOTTA, M. COPPETTI, P. RICCIARDI, F. CORELLI andA. TAFI
UV Raman microscopy of porphyrins immobilized in inorganic matrices 185
D.L.A. DE FARIA, V.R.L. CONSTANTINO, P.M. DIAS and D.N. BATCHELDER
Raman study of anthocyanins as a function of the temperature 187
L.G. QUAGLIANO, R.EI. GAWHARY, A. MASSACCI, F. PIETRINI andA. ·RIccl
Structural information on cofactor arrangement in photosynthetic reaction
centers obtained from time-resolved EPR spectra with magnetophotoselection 189
I.V. BOROVYKH, lB. KLENINA, I.I. PROSKURYAKOV, P. GAST andA.1. HOFF
