Table Of ContentSecond International Conference on Agriculture, 
Aquaculture and Animal Science 2015
 
 
 
 
 
 
Colombo, Sri Lanka 
 
28-29 December 2015 
 
 
 
 
 
 
Paper Proceedings of Agriculture, Aquaculture and  
Animal Science 2015
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
2015 
  International Center for Research & Development 
Colombo, Sri Lanka
Published by 
 
International Center for Research & Development 
 
International Center for Research & Development 
No. 858/6, 
Kaduwela Road,  
Thalangama North. 
 
 
[email protected] 
www.theicrd.org 
 
 
 
Printed in Sri Lanka 
 
December 2015 
 
 
 
ISBN 978-955-4543-32-4 
 
 
 
@ICRD December 2015 
All rights reserved.
Paper Proceedings of Agriculture, Animal Sciences and Aquaculture 2015(ISBN 978-955-4543-32-4) 
 
 
 
 
Agriculture, Animal Sciences and Aquaculture 2015 
 
 
 
 
Conference Advisers 
 
Prof. S.L. Ranamukhaarachchi 
Ph.D. (Agronomy – Cropping Sysems), Pennsylvania State University, USA 
 
Dr. Franz Uiblein 
Editor-in-chief, Marine Biology Research 
Guest Professor, University of Salzburg 
Principal Scientist IMR, Norway 
 
Conference Convener 
 
Prabhath Patabendi (Canada) 
 
ORGANIZERS 
 
 
International Center for Research & Development (ICRD) 
Chungnam National University, Republic of Korea 
 
International Scientific Committee 
 
Prof. Dr. Mahanama De Zoysa ( South Korea ) 
Prof. Rohana P Mahaliyanaarachchi ( Sri Lanka ) 
Dr. Kiran Kadam (USA) 
Prof. Dr. S.L. Ranamukhaarachchi (Thailand) 
Dr. Franz Uiblein ( Norway) 
Prof. S.D. Singh ( India ) 
Dr. Premachandra Wattage ( UK ) 
Dr. Bob Alexander ( USA ) 
Kennedy Shikami ( Kenya) 
Dr. Alec Woods ( New Zealand) 
Dr. Joseph Palmpilii ( India ) 
Dr. Biswajeet Pradhan ( Germany ) 
Dr. Cheng Liu ( Taiwan ) 
Prof. Karen Luz Y. Teves ( Phillipines ) 
Dr. Andrew Onwuemele ( Nigeria ) 
Dr. Prakasha Tiwari ( India ) 
Dr. G.R. Sahib ( India ) 
 
 
 
 
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Paper Proceedings of Agriculture, Animal Sciences and Aquaculture 2015(ISBN 978-955-4543-32-4) 
 
 
 
International Center for Research & Development 
 
 
 
 
 
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Paper Proceedings of Agriculture, Animal Sciences and Aquaculture 2015(ISBN 978-955-4543-32-4) 
 
 
Table of Contents 
 
 
 
Page 
KEYNOTE SPEACH  Publishing Authors 
No 
  Prof. Rohana P 
Agri tourism as an agri business opportunity for farming 
community in South Asia  Mahliyanaaarachchi  8 
 
 
PAPERS     
Evaluation of fruit and berry winter hardiness in the arid  Natalya Andrianova; 
1  10 
zone of Central Kazakhstan  Nazerke Shyntasova 
Potential of Biofilmed Biofertilizer for Efficient Crop 
2  J.P.H.U. Jayaneththi  19 
Production 
Bacillus megaterium as a Potential Biological Control 
Mana 
3  Agent to Suppress Grain Discoloration Disease of Rice  24 
Kanjanamaneesathian 
(Oryza sativa)  
4  Adequacy of simple agricultural drought indices  Devanmini Halwatura  30 
Pallampalli Sujathamma 
Value added sericulture products - Alternative marketing 
5  and Chinnakotla Leela  37 
strategies  Sushitha 
Seroepidemiology study on current equine viral diseases 
6  Roya Sadri  44 
in the stables of  breeding and racing horse  clubs in Iran 
Effects of Bovine Viral Diarrhea on animal reproduction 
7  Roya Sadri  49 
and economic impacts on semi-industrial breed in Iran 
Seasonal changes in ovarian follicle growth in Iran Viper 
8  Fatemeh Todehdehghan  56 
(Vipera albicornuta)  
Reproductive cycle of female Pit Viper (Gloydius halys 
9  Fatemeh Todehdehghan  63 
caucasicus) in Iran 
The Effect of plant densities and plant orientation on 
10  Abbas Biabani  70 
yield and yield components in wheat cultivars 
Depuru Bharathi and 
11  Present scenario of wildlife in India  76 
Burra Vijitha 
Investigation on optimum conditions of osmotic drying  Bahram Fathi 
12  81 
in the apricot slices  Achachlouei 
Effects of different levels of bitter vetch in starter feed 
13  on  performance  and  blood  metabolites  of  fat-tailed  H. Abdi-Benemar  88 
Moghani suckling lambs 
A note on the diversity and utility patterns of donkeys in 
14  Lawal Ribiu Maru  93 
selected local government areas of zamfara state, Nigeria 
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Paper Proceedings of Agriculture, Animal Sciences and Aquaculture 2015(ISBN 978-955-4543-32-4) 
 
 
  ABSTRACS     
Antibacterial  activities  of  “Lawsone”  as  active  Mahanama De Zoysa and 
1  100 
component isolated from Lawsonia inermis (henna)   S.H.S. Dananjaya 
Evaluation of fruit and berry winter hardiness in the arid 
2  Nazerke Shyntasova  100 
zone of Central Kazakhstan 
Effect of mixed diet of live feeds on growth performance 
3  and survival of Angel fish larvae (Pterophyllum scalare)  D.U. Hettiarachchi  101 
in indoor aquarium conditions 
Effects  of  elevated  temperature  on  oxidative  stress 
Nadirah Musa and Najiah 
4  response  and  biochemical  parameters  in  red  hybrid  101 
Musa 
tilapia, Oreochromis sp. 
Artificial Insemination for Cattle and Carabaos in Bicol 
5  Lourdita A. Llanto  102 
Region, Philippines:Its Implementation and Assessment 
Improved Method of Planting for Enhanced Seed Yield 
6  Basave Gowda  102 
in Chickpea 
Effects of Melaleuca cajuputi extracts as a natural feed 
7  additive  on  quality  of  African  catfish  (Clarias  Nurul Ulfah KARIM  103 
gariepinus)  
Mola-Carp Polyculture in Women Managed Household 
Md. Rafiqual Islam 
8  Pond: A New Window of Opportunity for Household  103 
Khan 
Nutrition and Employment 
Dietary supplementation with Prebiotics and Probiotics 
influence  Relative  Protein  digestibility  and  digestive  Mohammad Bodrul 
9  104 
enzyme  activities  in  Snakehead  (channa  striata)  Munir 
fingerlings 
Dairy  Cattle  Progesterone  Concentrations  in  Kuwait 
10  following Single  and Double Injection PGF2 Estrus  Muhammad Aali  104 
Synchronization Protocols 
Inclusion of Brown Seaweed (Sargassum crassifolium) 
11  as  a  Partial  Replacement  of  Fish  Meal  in  Diets  for  B.D.M. Mihiran  105 
Oreochromis niloticus Fingerlings 
Isolation  and  characterization  of  a  new  strain 
Propionibacterium  sp.  capable  of  producing 
12  Khansaa Badie Alkaddo  105 
polyhydroxyalkanoate (PHA) from waste glycerol, palm 
oil and different concentration of glucose 
Status of the rine shrimp, Artemia diversity in India: 
13  Natesan Munuswamy  106 
Distribution and Conservation Strategies 
Effect of feeding glycerol on healthy and economical 
14  Mubarik Mahmood  106 
milk production in ruminants.  
The  production  of  balanced  fodder  in  the  North 
15  Natalya Malitskaya  107 
Kazakhstan 
Inactivation of pathogenic luminous Vibrio harveyi from 
16  Pacific  white  shrimp  Penaeus  vannamei  by  phage  Najiah Musa  107 
cocktail 
Preliminary  studies  on  cryopreservation  of 
17  spermatophores  in  the  whiteleg  shrimp,  Litopenaeus  Selvakumar Narasimman  108 
vannamei 
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Paper Proceedings of Agriculture, Animal Sciences and Aquaculture 2015(ISBN 978-955-4543-32-4) 
 
 
Effects  of  bee  venom  on  behavioral  and  immune- R.M.C. Udayangani and 
18  108 
inflammatory responses in zebrafish (Danio rerio)   Mahanama De Zoysa 
Rhabdochona sp (Nematoda: Rhabdochnidae) from Tor 
19  Marina Hassan  119 
tambroides  in Kenyir Lake, Malaysia 
Refining Of Fish Oil From Hilsa (Tenualosa Ilisha) Fish 
20  By-Product, (Liquor) Available At Canned Fish Industry  S. Brintha  119 
In Sri Lanka 
Bivalve Fishery and Consumer Preference on Bivalves 
21  H.M.S.M. Wijerathna  110 
in Tambalagam Bay, Kinniya, Sri Lanka 
Heritability  Genetic  and  Phenotypic  Correlations  of 
22  Body Capacity Traits with Milk Yield in Sahiwal Cows  Musarrat Abbas Khan  110 
of Pakistan 
Evaluation Of Growth Parameters Of Freshwater Prawn 
23  (Macrobrachium Rosenbergii) Fed  On Formulated Diets  G. Hajarooba  111 
Supplemented With Probiotics 
B.Johannaross ; 
24  Empowerment Women through Sericulture  111 
 Ganta Savithri 
Effect of various cryoprotectants on sperm quality of  Dhanasekar 
25  112 
cobia, Rachycentron canadum  Krishnamoorthy 
Fish  Meal  Incorporated  Fish  Feed  Preparation  And 
26  k. Vickneswary  112 
Feeding Trials 
Diversity  of  Crabs  and  Shrimps  in  Thondaimanaru 
27  Piratheepa Sivakumar  113 
lagoon, Jaffna, Sri Lanka 
The  effect  of  Biofilmed  Biofertilizers  on  Rhizo-
28  Amila Henagamage  113 
remediation of potato 
The First record of Fusarium oxysporum infestation of  D.C.M. Kulatunga ; 
29  114 
laboratory zebrafish system  Mahanama De Zoysa 
Sustainable  Development  of  Sericulture  for  Indian 
30  Ganta Savithri  114 
Vibrant Rural Economy 
Studying  the  effect  of    wood  vinegar    and  nitrogen 
31  Barmaki Morteza  115 
fertilizer on  potato tubers production in Ardabil, Iran 
Antibacterial  properties  of  chitosan-silver  nano 
S.H.S. Dananjaya ; 
32  composite films and their potential use in water filtering  115 
Mahanama De Zoysa 
systems in aquaculture 
Green  Home  Technology  -  Need  For  Global  Leela Sushitha     
33  Chinnakotla  116 
Advancement 
Productivity of sweet patato (Ipomoea botatas (L.) Lam) 
as affected by NPK fertilizer rate, intra-row spacing and 
34  Mu’azu Abdu  116 
vine  length  in  the  northern  guinea  savanna  agro 
ecological zone of Nigeria.  
Selective  breeding  improved  the  performance  of 
35  H.K.A. Premachandra  117 
Yellowtail kingfish Seriola lalandi 
Dietary  Protein  and    Reproductive  Performance  of 
36  Roshada Hashim  117 
Channa striata (Bloch, 1793)  
Ex  situ  conservation  of fruit  and  berry  plants  in the 
37  Natalya Andrianova  118 
conditions of the northern desert of Kazakhstan 
Evaluation  Of  Growth  Performance  Of  Fighter  Fish 
38  R. Srikrishnan  118 
(Betta Splendens) Under Different Live Feeds 
 
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Paper Proceedings of Agriculture, Animal Sciences and Aquaculture 2015(ISBN 978-955-4543-32-4) 
 
 
 
KEYNOTE SPEECH  
 
 
Agri tourism as an agri business opportunity for farming community in South Asia 
 
Prof. Rohana P Mahliyanaaarachchi 
 
Senior Professor, Agri Business Management, Faculty of Agricultural Sciences,  
Sabaragamuwa University of Sri Lanka 
(Chief Editor – The Journal of Agricultural Sciences) 
 
Tourism industry has become the largest industry in the world at the end of year 2014 and in contrast 
development of the agricultural economy over the past three to four decades has been stagnant relatively 
to  the  faster  development  of  tourism  economy.  Over  the  past  years,  tourism  has  proven  to  be  a 
surprisingly  strong  and  pliant  economic  activity  and  a  fundamental  contributor  to  the  recovering 
economies in different sectors by generating billions of dollars and creating millions of new jobs. In this 
scenario, with the decline of productivity of farms and eventually total agricultural production due to 
human, environmental, climatic, technological and political factors, agri tourism has been recognized as 
an alternative activity to diversify the economic growth in agriculture sector. Diversification of income 
sources is the only alternative to stay in agriculture for farmers with small and medium sized farms due to 
high risks they are facing such as production or yield risk, market or price risk, regulatory risk, financial 
risk and personal risk. One strategy to overcome these risks practiced by some innovative farmers is 
adding agri tourism as an alternative business in their farms. It has a potential for building and expanding 
successful relationships between agriculture and tourism industries.  
 
However, there can also be challenges to agri tourism. It is important to examine and weigh all of the pros 
and cons before entering into any kind of agri tourism business. On the plus side, agri tourism can provide 
supplementary income to the farm business and serve as a means of employing staff beyond the regular 
growing season. For those who enjoy working with the public, agri tourism can be fun and rewarding. It 
is also an excellent way of promoting crops and value-added products produced in the farm. 
 
Among  the  challenging  factors  of  establishing  agri  tourism  business,  business  start-up  costs,  farm 
renovation cost in an existing farm, promotion of the business, and liability insurance, can be high. 
Operating an agri tourism business requires management and marketing skills different from those needed 
for running the typical farm. A tourism enterprise can require long, labor-intensive hours often focused on 
weekend and holiday traffic. Even for those with excellent social skills, dealing with the public can be 
challenging. Finally, agri tourism may significantly impact the entire farm family; be sure all members 
are “on board” before moving forward. 
 
Accordingly, there is growing interest in agri business profile on agri tourism. Both agriculture and 
tourism industries get benefits by developing agri tourism as a business.  Agri tourism as an agri business 
includes farm bed and breakfasts, roadside markets, farm restaurants, petting zoos, fee fishing, pony or 
horseback riding farm museums and many more agri tourism products. 
 
 
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Paper Proceedings of Agriculture, Animal Sciences and Aquaculture 2015(ISBN 978-955-4543-32-4) 
 
 
 
 
 
 
 
 
 
 
 
 
 
PAPERS 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
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Paper Proceedings of Agriculture, Animal Sciences and Aquaculture 2015(ISBN 978-955-4543-32-4) 
 
 
Evaluation of fruit and berry plant winter hardiness in the arid zone of Central 
Kazakhstan 
Natalya Andrianova1, Nazerke Shyntasova1, Tatyana Sirotina1 
 
1Committee of Science of Ministry of Education & Science of the Republic of Kazakhstan, Zhezkazgan 
Botanical Garden – branch of «Institute of Botany and Phytointroduction», Republic of Kazakhstan. 
 
Abstract 
The  study  presents  the  field  evaluation  of  winter  injuries  of  introduced  apple  and  pear  cultivars 
(2004/2005 - 2013/2014) and black currant cultivars (2009/2010 - 2013/2014) in the severe conditions of 
the arid zone of Central Kazakhstan (Zhezkazgan Botanical Garden). The crown’s winter damage degree 
of the pear and apple cultivars was significantly higher (p < 0.01) in 2005/2006 (minimal temperature -
39.2° C) and 2011/2012 (the highest sum of temperatures below zero 2033° C) than in the other seasons. 
Correlation between sum of temperatures below zero and the winter damage’s average degree of crowns 
of the apple, pear and black currant cultivars haы been established as high on the Cheddok’s scale, 
respectively  0.88  (p  < 0.01),  0.72  (p <  0.05)  and  0.89  (p  <  0.05).  Correlation  between  minimal 
temperatures and the winter damage’s average degree of apple, pear and black currant cultivars’ crowns 
hаs been established, respectively as -0.64 (noticeable, p < 0.05), -0.81 (high, p < 0.01) and -0.53 
(noticeable, ns). Winter injures of the pear and apple cultivars were significant higher than the black 
currant cultivars (p < 0.05). The results of the research have shown that ‘Arctica’ (Siberia), ‘Baganenok’ 
(Siberia), ‘Norland’ (Canada), ‘Norda’ (Canada) and ‘Desertnoe Petrova’ (Central Russia) apples; 
‘Zolotinka’  (Siberia),  ‘Barnaul’skaia  Krupnaia’  (Siberia),  ‘Krasulia’  (Ural),  ‘Petrovskaia’  (Central 
Russia) and ‘Lubava’ (Crimea) pears; ‘Altaiskaia Pozdniaia’, ‘Bariton’, ‘Galinka’, ‘Ksiusha’, ‘Lama’, 
‘Natasha’, ‘Rita’ (Siberia) black currants have suitable winter hardiness for the arid zone of Central 
Kazakhstan. 
 
Introduction 
 
Low temperature is a major environmental abiotic stress (Banuelos et al. 2008) that can inflict injury to 
plant tissues (Arora and Rowland 2011). The winter hardiness is genetically determined (Fircks and 
Verwijst 1993) so the use of cultivars with genetically determined winter hardiness seems to be the most 
desirable approach to avoid winter injury (Marini et al. 2001; Khanizadeh et al. 2003).  
Evaluation of fruit and berry plants from other climatic regions of the World to maintain living 
collection was initiated in Zhezkazgan Botanical Garden (ZBG) in 1939-1946. Today ZBG horticultural 
collection is composed of 350 accessions including 36 black currant cultivars (Ribes nigrum L., R. nigrum 
L. × R. dicusha Fisch. ex Turcz.), 120 apple cultivars (Malus domestica Borkh.) and 43 pear cultivars 
(Pyrus communis L. and P. communis L. × P. ussuriensis Maxim.) (Andrianova and Sirotina 2013). The 
objective of this study was to evaluate winter hardiness of the apple, pear and black cultivars and create 
the collection of winter hardy suitable plants for development of sustainable agriculture in the region.  
 
Materials and methods 
 
Site characteristics 
ZBG (47°48´ N, 67°43´  E, altitude 350 m) is situated in Central Kazakhstan.  Central Kazakhstan 
(Karaganda oblast) is the area in the centre of Eurasia continent almost equally-spaced from the Arctic 
and the Indian, the Atlantic and the Pacific oceans, in the northwest part of flat Central-North Turan sub 
province  in  the  sub  band  of  the  northern  deserts,  in  the  extremely  severe  for  plants  conditions 
(Akzhygitova et al. 2003). The climate is acutely continental and extremely droughty (Figure 1).  
 
10