role for hydrogen peroxide during abiotic and biotic stress signaling in plants

210 Pages·2008·9.13 MB·English

GHENT UNIVERSITY
FACULTY OF SCIENCES – DEPARTMENT MOLECULAR GENETICS
VIB DEPARTMENT OF PLANT SYSTEMS BIOLOGY

ROLE FOR HYDROGEN PEROXIDE DURING ABIOTIC AND BIOTIC
STRESS SIGNALING IN PLANTS

KORNEEL VANDENBROUCKE

Thesis submitted in partial fulfillment of the requirements for the degree of
Doctor (Ph.D.) of Science, Biotechnology
Academic year 2007-2008

Promoters:
Prof. Dr. Frank Van Breusegem
Prof. Dr. Dirk Inzé
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Cover: Artwork done by Armée de verre (Steven Theunis)
The scientist is not a person who gives the right answers, he's one who
asks the right questions

Claude Lévi-Strauss

Exam Committee
EXAM COMMITTEE

Promoters

Prof. Dr. Frank Van Breusegem (secretary) Department of Molecular Genetics,
Ghent University, Belgium
VIB Department of Plant Systems Biology

Prof. Dr. Dirk Inzé Department of Molecular Genetics,
Ghent University, Belgium
VIB Department of Plant Systems Biology

Promotion Committee

Prof. Dr. Ann Depicker (chairman) Department of Molecular Genetics,
Ghent University, Belgium
VIB Department of Plant Systems Biology

Prof. Dr. Yves Van De Peer Department of Molecular Genetics,
Ghent University, Belgium
VIB Department of Plant Systems Biology

Dr. Ir. Gerrit Beemster Department of Molecular Genetics,
Ghent University, Belgium
VIB Department of Plant Systems Biology

Prof. Dr. Ir. Monica Höfte Department of Crop Protection
Laboratory of Phytopathology,
Faculty of Bio-engineering,
Ghent University, Belgium

Dr. Michael Metzlaff Bayer BioScience N.V.,
Ghent Belgium

Prof. Dr. Graham Noctor Institut de Biotechnologie des Plantes,
Université de Paris, France

Prof. Dr. Yves Guisez Department of Biology
Faculty of Sciences
Antwerp University, Belgium

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Table of Contents
TABLE OF CONTENTS

Exam committee 5
Table of contents 7
List of abbreviations 9

Research Objectives 13

PART I GENERAL INTRODUCTION
Chapter 1 15
How do plants deal with abiotic stress? Meta-analysis on transgenic plants with increased stress
tolerance
Abstract
Introduction
Conclusions and Perspectives
References

PART II HYDROGEN PEROXIDE SIGNALING IN PLANTS
Chapter 2 49
Hydrogen peroxide-induced gene expression across kingdoms: A comparative analysis
Abstract
Introduction
Results and Discussion
Conclusions
Materials and Methods
References

Chapter 3 69
Identification of genes involved in plant defense against necrotrophic pathogens by using virus-
induced gene silencing
Abstract
Introduction
Results
Discussion
Conclusions and Perspectives
Materials and Methods
References

Chapter 4 97
Ectopic expression of the WRKY15 transcription factor in Arabidopsis increases tolerance to
oxidative stress
Abstract
Introduction
Results
Discussion
Conclusions and Perspectives
Materials and Methods
References
7
Table of Contents
PART III DROUGHT STRESS IN ARABIDOPSIS
Chapter 5 123
Development and evaluation of a semi-automated system to monitor the growth of Arabidopsis
plants during drought stress
Abstract
Introduction
Results
Discussion
Conclusions and Perspectives
Materials and Methods
References
Supplementary data

Chapter 6 145
Molecular phenotyping of drought tolerant Arabidopsis plants overexpressing a galactinol
synthase, GOLS2, reveals a role for myo-inositol
Abstract
Introduction
Results and Discussion
Perspectives
Materials and Methods
References

PART IV GENERAL DISCUSSION AND SUMMARY OF THE THESIS
Chapter 7 163
General Discussion, Conclusions and Perspectives

Chapter 8 167
Summary / Samenvatting

PART V ADDENDUM
Supplementary Tables 181

Curriculum Vitae 205
Dankwoord 207

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List of Abbreviations
LIST OF ABBREVIATIONS
26S RSU 26S proteasome regulatory subunit
A ABA Abscisic acid
AFLP Amplified fragment length polymorphism
AGI Arabidopsis Genome Initiative
Agrobacterium Agrobacterium tumefaciens
APX Ascorbate peroxidase
Arabidopsis Arabidopsis thaliana
ATP Adenine triphosphate
B BLAST Basic local alignment and search tool
C CAT Catalase
cDNA Complementary DNA
Col Columbia ecotype of Arabidopsis thaliana
D DNA Deoxyribonucleic acid
DRP DNA-afhankelijk RNA polymerase
DW Dry Weight
E EST Expressed sequence tag
F FC Fold Change
FW Fresh Weight
G GO Gene ontology
GPX Glutathione peroxidise
GST Gene-specific tag
GTP Guanine triphosphate
H H O Hydrogen peroxide
2 2
HRM H O -response matrix
2 2
HSP Heat shock protein
M MAPK Mitogen-activated protein kinase
MI Myo-inositol
mRNA Messenger RNA
O OE Overexpression
P PCR Polymerase chain reaction
PDS Phyotene desaturase
Ph.D. Phylosophiae doctor
POF Protein with obscure features
R RNA Ribonucleic acid
ROS Reactive oxygen species
S SDR Short-chain dehydrogenase/reductase
SOD Superoxide dismutase
STG Stress tolerance gene
T T-DNA Transferred DNA
TF Transcription factor
ThrRS Threonyl tRNA synthetase
tRNA Transfer RNA
TRV Tobacco rattle virus
U Ub Ubiquitin
V VIGS Virus-induced gene silencing
W WIWAM Weighing imaging and watering machine
WT Wild type
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