RNA Polymerase and Associated Factors Part B

605 Pages·1996·10.731 MB·English

by  Abelson J. (ed.), Simon M. (ed.), Adhya S. (ed.)

Preface
One cannot fully understand the biology of a cell without understanding
the central role that gene expression and its regulation play. RNA polymer-
ase was discovered in eukaryotes in 1959 and in prokaryotes in 1960, and
the subject of transcription regulation was reported in the early 1960s.
Although many breakthrough experiments were performed in the 1970s,
there has been, unquestionably, an explosion in our knowledge in the field
since the 1980s, thanks to the rapid development and use of powerful
genetic, biochemical, and physical techniques. As a result, many plausible,
sometimes unexpected, ideas have been generated. More encouragingly,
some of the ideas have been accepted.
Volumes 372 and 274 of Methods in Enzymology cover, for the first
time, methods and other analytical approaches for the study of transcription
and its regulation in prokaryotes and eukaryotes. The chapters in these
two volumes describe steps of transcription; component machinery and
their specificity; purification, assays, and properties of RNA polymerases
and their intrinsic and extrinsic (including regulatory) factors that guide
transcription initiation, elongation, and termination; and the assembly of
RNA polymerase holoenzymes and many regulatory protein-protein and
nucleoprotein complexes, including chromatins. A few chapters dealing
with specialized techniques analyzing transcriptional regulation are also in-
cluded.
These volumes will help further exploration of how transcription con-
trols cellular adaptation, development, and differentiation. We underscore
the importance of DNA-protein interactions in studying transcription and
its regulation, a subject covered in Volume 208 of this series.
SANKAR ADHYA
iivx
Contributors to Volume 274
elcitrA numbers are ni parentheses gniwollof the names of .srotubirtnoc
snoitailiffA listed are .tnerruc
CHRISTOPHER C. ADAMS (22), Department R. WERDNA BYRD (30), Macromolecular
of Biochemistry and Molecular Biology, NMR Section, ABL-Basic Research Pro-
Pennsylvania State University, ytisrevinU ,marg NCI-Frederick Cancer hcraeseR dna
Park, Pennsylvania 20861 Development ,retneC ,kcirederF Maryland
AMANDA S. ALTIERI (30), ralucelomorcaM 20712
NMR Section, ABL-Basic Research Pro- MICHAEL CAREY (11), Department of -igoloiB
,marg NCI-Frederick Cancer Research dna lac ,yrtsimehC UCLA School of ,enicideM
Development ,retneC ,kcirederF Maryland Los Angeles, California 59009
20712
CATHLEEN L. CHAN (27), Department of -otS
ORIJIET OSA (33), Program in Molecular and matology, University of California, naS
lleC Biology, Oklahoma Medical hcraeseR ,ocsicnarF California 52049
Foundation, Oklahoma City, Oklahoma
40137 DIPANKAR CHATTERJI (35), Center for -ulleC
ral and Molecular Biology, 005-dabaredyH
NELIAS KIRAB (29), Department of Biochem-
700 (A.P.), India
yrtsi dna Molecular Biology, ytisrevinU of
South Alabama College of ,enicideM Mo- SAMIT CHATTOPADHYAY (30), retneC for -naC
,elib Alabama 88663 rec ,hcraeseR Department of Biology, Mas-
sttesuhcas Institute of Technology, Cam-
M1CHELLE CRAIG BARTON (24), Department
,egdirb sttesuhcassaM 83120
of raluceloM ,sciteneG ytisrevinU of -nicniC
,itan ,itannicniC Ohio 76254 IAUHNAIT IHC (11), Department of lacigoloiB
,yrtsimehC UCLA School of ,enicideM Los
EZNATSNOC REFINOB (18), Institut ftir Biolo-
Angeles, California 59009
eig lII, Albert-Ludwigs-Universitdt Frei-
,grub D-79104 ,grubierF Germany CHENG-MING CHIANG (6), Department of
,yrtsimehcoiB University of Illinob at -rU
UWE BORGMEYER (18), Center for Molecular
,ngiapmahC-anab ,anabrU Illinois 10816
Neurobiology ,II University of Hamburg,
15202-D Hamburg, Germany GNUOYE1M IOHC (2), Committee on Develop-
1EGRES VOHKUROB (25, 26), Department of mental Biology, ytisrevinU of ,ogacihC Chi-
Microbiology and Immunology, State -inU ,ogac Illinois 73606
ytisrev of New York, Health Science retneC NOYH E. Cnov (1), Department of raluceloM
at Brooklyn, Brooklyn, New York 30211 Biology, Odense University, DK-5230
LEAHCIM ZTIWONERB (36), Department of esnedO ,M Denmark 5524-29802
Biochemistry, Albert Einstein College of DIVAD J. KRALC (19), Laboratory of ralulleC
,enicideM Bronx, New York 16401 and Developmental Biology, National -itsnI
RICHARD R. BURGESS (39), McArdle Labora- tute of Diabetes and Digestive and Kidney
tory for Cancer Research, University of ,sesaesiD National Institutes of ,htlaeH
,nosidaM-nisnocsiW Madison, Wisconsin ,adsehteB Maryland 29802
60735 JOAN WELIKY CONAWAY (33), Program in
SANDEEP BURMA (3), Eukaryotic Gene Ex- Molecular dna Cell Biology, Oklahoma
pression Laboratory, National Institute of Medical Research Foundation, Oklahoma
Immunology, New Delhi-llO067, India ,ytiC Oklahoma 40137
xii CONTRIBUTORS TO VOLUME 274
RONALD C. CONAWAY (33), Program ni Mo- GARY FELSENFELI) (19), Laboratory of Mo-
ralucel and Cell Biology, Oklahoma Medi- ralucel Biology, National Institute of -ebaiD
lac Research Foundation, Oklahoma ,ytiC set and Digestive dna Kidney ,sesaesiD
Oklahoma 40137 National Institutes of Health, ,adsehteB
NINA ONITNATSOC (30), Laboratory of -orhC Maryland 29802
mosome Biology and ABL-Basic hcraeseR MICHAEL FRITSCH (9), Laboratory of Bio-
,margorP kcirederF-1CN Cancer hcraeseR ,yrtsimehc National Cancer Institute, -aN
and Development ,retneC ,kcirederF Mary- tional Institutes of ,htlaeH ,adsehteB Mary-
land 20712 land 29802
JACQUES COT 51 (22), Department of Biochem-
Hui GE (6), Laboratory of Molecular Embry-
istry and Molecular Biology, Pennsylvania
ology, National Institute of Child Health
State ,ytisrevinU University Park, Pennsyl-
and Human Development, National Insti-
ainav 20861
setut of Health, Bethesda, Maryland 29802
DONALD COURT (30), Laboratory of Chromo-
JEFFREY S. GERBER (5), Laboratory of -celoM
some Biology and ABL-Basic Research
ralu dna ralulleC Biology, National etutitsnI
Program, NCl-Frederick Cancer hcraeseR
of Diabetes and Digestive and Kidney
and Development ,retneC ,kcirederF Mary-
,sesaesiD National Institutes of ,htlaeH
land 20712
,adsehteB Maryland 29802
ROBIN YELSSORC (30), Department of -orciM
biology, University of Connecticut School BALARAM GHOSH (29, 30), Center for Bio-
of Medicine, Farmington, Connecticut chemical Technology, Council of cifitneicS
and Industrial Research, Delhi ,700011
03O6O
aidnI
XING DAI (2), Department of Biochemistry
and Molecular Biology, University of -ihC M. ALEXANDRA GLUCKSMANN-KuIs (2), -eD
,ogac Chicago, Illinois 73606 partment of Molecular Genetics and Cell
Biology, University of ,ogacihC ,ogacihC
AsIs DAS (29, 30), Department of -loiborciM
Illinois 73606
ogy, University of Connecticut School of
,enicideM Farmington, Connecticut 03060 ALEX GOLDFARB (25, 26), Public Health -eR
JOSEPH DEVITo (30), Laboratory of Myco- hcraes ,etutitsnI New York, New York 610Ol
,airetcab Center for Biologics Evaluation NORA GOOSEN (4), Laboratory of raluceloM
and Research, FDA, Bethesda, Maryland ,'sciteneG Leiden Institute of ,yrtsimehC
29802 Leiden ,ytisrevinU Gorlaeus ,seirotarobaL
RONNY DRAPKIN (7), Department of Bio- 2300 RA Leiden, The sdnalrehteN
,yrtsimehc Howard Hughes Medical Insti-
VIJAYA GOPAL (35), Center for Cellular and
,etut Robert Wood Johnson Medical ,loohcS
Molecular Biology, Hyderabad-500 700
ytisrevinU of Medicine and Dentistry of
(A.P.), India
New ,yesreJ ,yawatacsiP New Jersey 45880
JACK GREENBLATF (10), Banting and Best -eD
RICHARD H. EBRIGHT (37), Department of
partment of lacideM ,hcraeseR Department
Chemistry and Waksman Institute, Rutgers
of ,yrtsimehcoiB and Department of -celoM
,ytisrevinU New Brunswick, New Jersey
ular and lacideM ,sciteneG ytisrevinU of -oT
5588O
,otnor Toronto, Ontario M5G 1L6, adanaC
ALED M. EDWARDS (32), Cancer hcraeseR
Group, Institute for Molecular Biology dna SAMAN HAB1B (3), Eukaryotic Gene Expres-
,ygolonhcetoiB McMaster ,ytisrevinU Ham- sion Laboratory, National Institute of Im-
,notli Ontario L8N 3Z5, adanaC munology, New ,760Oll-ihleD India
BEVERLY M. EMERSON (24), Regulatory Biol- NAHTANOJ HAM (14), Eisai London hcraeseR
ogy Laboratory, ehT Salk Institute for Bio- ,seirotarobaL University College London,
lacigol ,seidutS La Jolla, California 73029 London WC1E 6BT, United Kingdom
CONTRIBUTORS TO VOLUME 274 xiii
MICHELLE M. HANNA (31), Departments of CAROLINE M. KANE (32), Department of Mo-
Chemistry and Biochemistry, University of lecular and Cell Biology, University of Cali-
Oklahoma, Norman, Oklahoma 73109 fornia, Berkeley, California 94720
8EYED E. HASNAIN (3), Eukaryotic Gene Ex- MIKHAIL KASHLEV (26), Public Health Re-
pression Laboratory, National Institute of search Institute, New York, New York 10016
Immunology, New Delhi-llO067, India MICHAEL P. KLADDE (17), Department of Bio-
chemistry and Molecular Biology, Pennsyl-
TOMASZ HEYDUK (37), Department of Bio-
vania State University, University Park,
chemistry and Molecular Biology, .tS Louis
Pennsylvania 16802
University School of Medicine, .tS Louis,
Missouri 63104 RICHARD D. KLAUSNER (33), Cell Biology and
Metabolism Branch, National Institute of
DEBORAH M. HINTON (5), Laboratory of Mo-
Child Health and Human Development,
lecular and Cellular Biology, National Insti-
National Institutes of Health, Bethesda,
tute of Diabetes and Digestive and Kidney
Maryland 20892
Diseases, National Institutes of Health,
Bethesda, Maryland 20892 NATALIA KOMISSAROVA (26), Public Health
Research Institute, New York, New York
H. CHRISTOPH HOEFER (18), Institutfar Biolo- 10016
gie llI, Albert-Ludwigs-Universiti~t Frei-
JOSEPH S. KRAKOW (38), Department of Bio-
burg, D-79104 Freiburg, Germany
logical Sciences, Hunter College of the City
KC1REDOR HORI (1 0, Department of Biologi- University of New York, New York, New
cal Chernistry, UCLA School of Medicine, York 10021
Los Angeles, California 90095
ROBERT LANDICK (27), Departments of Biol-
JINZHAO HOU (8), Arris Pharmaceutical Cor- ogy and Biochemistry and Molecular Bio-
poration, South San Francisco, California physics, Washington University, .tS Louis,
94080 Missouri 63130
MARK HSIEH (36), Department of Biochemis- W. MARSTON LINEHAN (33), Urologic Oncol-
try, Albert Einstein College of Medicine, ogy Section, Surgery Branch, National Can-
Bronx, New York 10461 cer Institute, National Institutes of Health,
Bethesda, Maryland 20892
SAIHFV'AM C. HUBER (18), Institutfiir Biologie
Ill, Albert-Ludwigs-Universitiit Freiburg, JIANYING LuO (38), Surgical Laboratory,
Children's Hospital, Harvard Medical
D-79104 Freiburg, Germany
School, Boston, Massachusetts 02115
C. JAMES INOLES (10), Banting and Best De-
GN1XEUY MA (37), Department of Chemistry
partment of Medical Research, Department
and Waksman Institute, Rutgers University,
of Biochemistry, and Department of Molec-
New Brunswick, New Jersey 08855
ular and Medical Genetics, University of
Toronto, Toronto, Ontario M5G 1L6, EDIO MALDONADO (7), Department of Bio-
chemistry, Howard Hughes Medical Insti-
Canada
tute, Robert Wood Johnson Medical School,
ANJALI JA1N (3), Eukaryotic Gene Expression
University of Medicine and Dentistry of
Laboratory, National Institute of Immunol-
New Jersey, Piscataway, New Jersey 08854
ogy, New Delhi-llO067, India
TOM S1TAINAM (13), Department of Molecular
PAUL JEDLICKA (9), Laboratory of Biochemis- and Cell Biology, Harvard University, Cam-
try, National Cancer Institute, National bridge, Massachusetts 02138
Institutes of Health, Bethesda, Maryland
NYLSOR MARCH-AMEGADZIE (5), Laboratory
20892
of Molecular and Cellular Biology, National
LI-JUNG JUAN (22), lntercollege Program in Institute of Diabetes and Digestive and Kid-
Genetics, Pennsylvania State University, ney Diseases, National Institutes of Health,
University Park, Pennsylvania 16802 Bethesda, Maryland 20892
xiv CONTRIBUTORS TO VOLUME 274
TSENRE ZENITRAM (6), Laboratory of Bio- BEDAHAM LAP (30), Department of -iborciM
chemistry and Molecular Biology, ehT ology, University of tucitcennoC School of
Rockefeller University, New York, New ,enicideM Farmington, Connecticut 03060
York 12001 MINRA ESUAP (33), Cell Biology and -obateM
MARIE MAZZULLA (30), Macromolecular lism Branch, National Institute of Child
NMR Section, ABL-Basic Research Pro- Health and Human Development, National
,marg NCI-Frederick Cancer Research and Institutes of Health, Bethesda, Maryland
Development ,retneC Frederick, Maryland 298O2
20712 GNUS PYo (11), Department of Biological
NEVETS L. McKNI~HT (8), Tularik Incorpo- ,yrtsimehC UCLA School of ,enicideM Los
,detar South San Francisco, California Angeles, California 59009
08049 MAILLIW REES (30), Howard Hughes lacideM
JAIME OARCIA MENA (30), Department of ,etutitsnI National Jewish Center for Immu-
Microbiology, University of Connecticut nology and Respiratory Medicine, ,revneD
School of ,enicideM Farmington, -itcennoC odaroloC 60208
cut 03060 YNNAD GREBNIER (7), Department of Bio-
,yrtsimehc Howard Hughes Medical Insti-
ALITA RELLIM (2), Department of -simehcoiB
,etut Robert Wood Johnson Medical ,loohcS
yrt and Molecular Biology, University of
ytisrevinU of Medicine dna Dentistry of
,ogacihC ,ogacihC Illinois 73606
New ,yesreJ ,yawatacsiP New Jersey 45880
GAKU IHCUGUZIM (9), Laboratory of Bio-
JOHN P. NOSDRAHCIR (28), Department of
,yrtsimehc National Cancer Institute, Na-
,yrtsimehC Indiana ,ytisrevinU Blooming-
tional Institutes of ,htlaeH ,adsehteB Mary-
ton, Indiana 50474
land 29802
LisLoaT- NOSDRAHCIR (28), Department of
IHSEKAT ONUZIM (21), Laboratory of -celoM
,yrtsimehC lndiana ,ytisrevinU Blooming-
ular Microbiology, School of ,erutlucirgA
,not Indiana 50474
Nagoya ,ytisrevinU Nagoya ,464 Japan
TREBOR G. ROEDER (6), Laboratory of Bio-
AHSAPIB EEILIEHKUM (3), Eukaryotic Gene
chemistry and Molecular Biology, ehT
Expression Laboratory, National Institute
Rockefeller University, New York, New
of Immunology, New Delhi-llO067, India
York 12001
MAILLIW EKZTAWON (28), Department of
AICUL B. SENED-NAMHTOR (2), Department
,yrtsimehC Indiana ,ytisrevinU Blooming-
of Molecular Genetics and Cell Biology,
ton, Indiana 50474
ytisrevinU of Chicago, Chicago, Illinois
YNEGVE NUDLER (26), Public Health Re- 73606
hcraes ,etutitsnI New York, New York 61001 DLARAH GISSEREUAS (18), Molecular Neuro-
ARUAL P. O'NEILL (15), Chromatin and Gene biology Laboratory, ehT Salk ,etutitsnI La
Expression Group, Anatomy ,tnemtrapeD ,alloJ California 73029
ytisrevinU of Birmingham Medical ,loohcS SAMOHT D. REDIENHCS (34), Laboratory of
,notsabgdE Birmingham B15 2TT, United Mathematical Biology, Frederick Cancer
Kingdom Research and Development Center, -aN
SARDNA ZSORO (9), Laboratory of Biochem- tional Cancer Institute, Frederick, Mary-
,yrtsi National Cancer Institute, National land 20712
Institutes of Health, Bethesda, Maryland NITNATSNOK VONIREVES (26), Public Health
29802 hcraeseR Institute, New York, New York
SAMOHT A. SEHGUH-NEWO (22), Department 61001
of Biochemistry and Molecular Biology, MIRAK A. FIRAHS (38), Department of -celoM
Pennsylvania State University, ytisrevinU ular ,ygolocamrahP Albert Einstein egelloC
Park, Pennsylvania 20861 of ,enicideM Bronx, New York 16401
CONTRIBUTORS TO VOLUME 274 xv
MRIDULA SHARMA (5), Laboratory of -celoM BRYAN M. TURNER (15), Chromatin and Gene
ular and ralulleC Biology, National etutitsnI Expression Group, Anatomy ,tnemtrapeD
of Diabetes and Digestive and Kidney ytisrevinU of Birmingham Medical ,loohcS
,sesaesiD National Institutes of Health, ,notsabgdE Birmingham 51B 2TT, United
,adsehteB Maryland 29802 Kingdom
ROBERT T. NOSPMIS (17), Department of Bio- CHIHARU UEGUCHI (21), Laboratory of Mo-
yrtsimehc and Molecular Biology, Pennsyl- ralucel Microbiology, School of Agricul-
vania State University, University Park, ,erut Nagoya ,ytisrevinU Nagoya ,464 Japan
Pennsylvania 20861 KIYOE URA (20), Laboratory of Molecular
ALBRECHT E. SIPPEL (18), Institutfar eigoloiB Embryology, National Institute of Child
III, Albert-Ludwigs-Universitiit ,grubierF htlaeH dna Human Development, National
40197-D ,grubierF Germany Institutes of Health, Bethesda, Maryland
GERTRUD STEGER (14), Institut far ,eigoloriV 29802
tiitisrevinU zu KOln, 50935 Cologne, RHEA T. UTLEY (22), Department of Bio-
ynamreG yrtsimehc dna Molecular Biology, Pennsyl-
ARIBERT STILE (18), Institut far Biologie vania State University, University Park,
III, Albert-Ludwigs-Universitiit ,grubierF ainavlysnneP 20861
40197-D ,grubierF Germany RAFAEL VALCARCEL (12), EMBL, D-69117
VAS1LY M. STUDITSKY (19), Laboratory of ,grebledieH Germany
raluceloM Biology, National Institute of -iD PIETER NAV ED PUTI'E (4), Laboratory of Mo-
seteba and Digestive and Kidney ,sesaesiD ralucel ,sciteneG Leiden Institute of -mehC
National Institutes of Health, ,adsehteB ,yrtsi Leiden ,ytisrevinU Gorlaeus Labora-
Maryland 29802 ,seirot 2300 RA Leiden, ehT sdnalrehteN
HENDRIK G. STUNNENBERG (12), EMBL, PETER NAV ULSEN (4), Laboratory of -uceloM
71196-D ,grebledieH Germany ral ,sciteneG Leiden Institute of ,yrtsimehC
HONG TANG (37), Department of yrtsimehC Leiden ,ytisrevinU Gorlaeus ,seirotarobaL
and Waksman ,etutitsnI Rutgers ,ytisrevinU 0032 RA Leiden, ehT sdnalrehteN
New Brunswick, New Jersey 55880 PETER H. NOV HIPPEL (30), Institute of -celoM
DEAN TANTIN (11), Molecular Biology Insti- raM Biology, Department of ,yrtsimehC
,etut ytisrevinU of ,ainrofilaC Los ,selegnA ytisrevinU of Oregon, Eugene, Oregon
Los Angeles, California 59009 3O479
DIMITRIS THANOS (13), Department of Bio- DAGUANG WANG (27), Department of Biol-
chemistry dna Molecular Biophysics, -oC ogy, Washington ,ytisrevinU .tS Louis, Mis-
lumbia ,ytisrevinU New York, New York souri 03136
23001 WILLIAM WHALEN (29, 30), Laboratory of
FRITZ THOMA (16), Institut far ,eigoloiblleZ raluceloM ,ygoloriV National recnaC -itsnI
ehcsissOnegdiE Technische Hochschule, ,etut National setutitsnI of ,htlaeH ,adsehteB
,grebreggnOH-HTE 3908-HC Zurich, Swit- Maryland 29802
dnalrez JAN IKSWEINSIW (9), Laboratory of Biochem-
NANCY E. NOSPMOHT (39), McArdle Labora- ,yrtsi National recnaC ,etutitsnI National In-
tory for Cancer Research, University of stitutes of Health, Bethesda, Maryland
,nosidaM-nisnocsiW Madison, Wisconsin 298O2
60735 ALAN P. WOLFFE (20), Laboratory of -uceloM
TOSHIO TSUKIYAMA (23), Laboratory of Bio- ral Embryology, National Institute of dlihC
,yrtsimehc National Cancer Institute, -aN htlaeH dna Human Development, National
tional Institutes of ,htlaeH ,adsehteB Mary- Institutes of Health, Bethesda, Maryland
land 29802 29802
xvi CONTRIBUTORS TO VOLUME 274
ANYTSYRK WOLSKA (30), Institute of -iborciM MOSHE YANIV (14), Unit~ sed Virus Onco-
ology, University of Warsaw, Warsaw ,46 ,senOg Ddpartement sed ,seigolonhcetoiB
Poland Institut ,ruetsaP 42757 Paris, ecnarF
WEN-CHEN YEH (8), Department of ,ygoloiB
JERRY L. WORKMAN (22), Department of Bio- Johns Hopkins University, Baltimore,
yrtsimehc and Molecular Biology and -neC Maryland 81212
ret for Gene ,noitalugeR Pennsylvania etatS N1M ZHONG (9), Laboratory of ,yrtsimehcoiB
,ytisrevinU University Park, Pennsylvania National Cancer Institute, National Insti-
20861 tutes of Health, ,adsehteB Maryland 29802
LAURENCE ZULIANELLO (4), Laboratory of
CARL WU (9, 23), Laboratory of Biochemis- Molecular Genetics, Leiden Institute of
try, National Cancer Institute, National Chemistry, Leiden University, Gorlaeus
Institutes of Health, Bethesda, Maryland ,seirotarobaL 2300 RA Leiden, ehT -rehteN
29802 sdnal
METHODS IN ENZYMOLOGY
EMULOV I. Preparation and Assay of Enzymes
Edited by SIDNEY P. KCIWOLOC DNA NATHAN 0. KAPLAN
EMULOV lI. Preparation and Assay of Enzymes
Edited yb SIDNEY P. KCIWOLOC DNA NATHAN 0. KAPLAN
EMULOV III. Preparation and Assay of Substrates
by Edited SIDNEY P. KCIWOLOC DNA NATHAN O. KAPLAN
EMULOV IV. Special Techniques for the Enzymologist
Edited by SIDNEY P. KCIWOLOC DNA NATHAN 0. KAPLAN
EMULOV V. Preparation and Assay of Enzymes
Edited yb SIDNEY P. KCIWOLOC DNA NATHAN 0. KAPLAN
EMULOV VI. Preparation and Assay of Enzymes (Continued)
Preparation and Assay of Substrates
Special Techniques
Edited yb SIDNEY P. KCIWOLOC DNA NATHAN 0. KAPLAN
EMULOV VII. Cumulative Subject Index
by Edited SIDNEY P. KClWOLOC DNA NATHAN O. KAPLAN
EMULOV VIII. Complex Carbohydrates
Edited by ELIZABETH F. NEUFELD DNA VICTOR GRUBSNIG
EMULOV IX. Carbohydrate Metabolism
Edited yb WILLIS A. WOOD
EMULOV X. Oxidation and Phosphorylation
Edited by RONALD W. KOORBATSE DNA MAYNARD E. NAMLLUP
EMULOV XI. Enzyme Structure
Edited yb C. H. W. HIRS
EMULOV XII. Nucleic Acids (Parts A and B)
by Edited ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XIII. Citric Acid Cycle
Edited yb J. M. NIETSNEWOL
EMULOV XIV. Lipids
Edited by J. M. NIETSNEWOL
EMULOV XV. Steroids and Terpenoids
Edited by DNOMYAR B. NOTYALC
EMULOV XVI. Fast Reactions
Edited by HTENNEK NITSUK
xix
XX METHODS IN ENZYMOLOGY
EMULOV XVII. Metabolism of Amino Acids and Amines (Parts A and B)
Edited yb HERBERT TABOR DNA CELIA WHITE TABOR
EMULOV XVIII. Vitamins and Coenzymes (Parts A, B, and C)
Edited by DONALD B. McCORMICK DNA LEMUEL D. WRIGHT
EMULOV XIX. Proteolytic Enzymes
Edited by GERTRUDE E. NNAMLREP DNA LASZLO DNAROL
EMULOV XX. Nucleic Acids and Protein Synthesis (Part C)
Edited by KIVtE MOLDAVE DNA ECNERWAL NAMSSORG
EMULOV XXI. Nucleic Acids (Part D)
Edited by ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXII. Enzyme Purification and Related Techniques
Edited by WILLIAM B. YBOKAJ
EMULOV XXIII. Photosynthesis (Part A)
Edited by SAN PIETRO ANTHONY
EMULOV XXIV. Photosynthesis and Nitrogen Fixation (Part B)
Edited by ANTHONY NAS PIETRO
EMULOV XXV. Enzyme Structure (Part B)
Edited by C. H. W. HIRS DNA SERGE N. FEEHSAMIT
EMULOV XXVI. Enzyme Structure (Part C)
Edited by C. H. W. HIRS DNA SERGE N. FFEHSAMIT
EMULOV XXVII. Enzyme Structure (Part D)
Edited yb C. H. W. HIRS DNA SERGE N. EFEHSAMIT
EMULOV XXVIII. Complex Carbohydrates (Part B)
Edited by VICTOR GRUBSNIG
EMULOV XXIX. Nucleic Acids and Protein Synthesis (Part E)
Edited by ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXX. Nucleic Acids and Protein Synthesis (Part F)
Edited yb KIVIE MOLDAVE DNA ECNERWAL NAMSSORG
EMULOV XXXI. Biomembranes (Part A)
Edited by SIDNEY REHCSIELF DNA LESTER PACKER
EMULOV XXXII. Biomembranes (Part B)
Edited yb FLEISCHER SIDNEY DNA PACKER LESTER
EMULOV XXXIII. Cumulative Subject Index Volumes I-XXX
Edited by MARTHA G. DENNIS DNA EDWARD A. DENNIS
EMULOV XXXIV. Affinity Techniques (Enzyme Purification: Part B)
Edited by WILLIAM B. JAKOBY DNA MEIR WILCHEK
EMULOV XXXV. Lipids (Part B)
Edited yb JOHN M. NIETSNEWOL
EMULOV XXXVI. Hormone Action (Part A: Steroid Hormones)
Edited by BERT W. O'MALLEY DNA JOEL G. HARDMAN
METHODS NI ENZYMOLOGY xxi
EMULOV XXXVII. Hormone Action (Part B: Peptide Hormones)
Edited yb TREB W. O'MALLEY DNA LEOJ G. NAMDRAH
EMULOV XXXVIII. Hormone Action (Part C: Cyclic Nucleotides)
Edited yb JOEL G. NAMDRAH DNA TREB W. YELLAM'O
EMULOV XXXIX. Hormone Action (Part D: Isolated Cells, Tissues, and Organ
Systems)
Edited yb LEOJ G. NAMDRAH DNA TREB W. YELLAM'O
EMULOV XL. Hormone Action (Part E: Nuclear Structure and Function)
Edited yb TREB W. O'MALLEY DNA LEOJ G. NAMDRAH
EMULOV XLI. Carbohydrate Metabolism (Part B)
Edited by W. A. DOOW
EMULOV XLII. Carbohydrate Metabolism (Part C)
Edited yb .W A. Wood
EMULOV XLIII. Antibiotics
Edited yb NHOJ H. HSAH
EMULOV XLIV. Immobilized Enzymes
Edited yb SUALK HCABSOM
EMULOV XLV. Proteolytic Enzymes (Part B)
Edited yb OLZSAL DNAROL
EMULOV XLVI. Affinity Labeling
Edited by MAILLIW B. YBOKAJ DNA MEIR KEHCLIW
EMULOV XLVII. Enzyme Structure (Part E)
Edited by .C H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XLVIII. Enzyme Structure (Part F)
Edited yb .C H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XLIX. Enzyme Structure (Part G)
Edited by .C H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV L. Complex Carbohydrates (Part C)
Edited by ROTCIV GRUBSNIG
EMULOV LI. Purine and Pyrimidine Nucleotide Metabolism
Edited yb PATRICIA A. HOFFEE DNA MARY ELLEN JONES
EMULOV LII. Biomembranes (Part C: Biological Oxidations)
Edited by SIDNEY FLEISCHER DNA LESTER PACKER
EMULOV LIII. Biomembranes (Part D: Biological Oxidations)
Edited yb SIDNEY FLEISCHER DNA LESTER PACKER
EMULOV LIV. Biomembranes (Part E: Biological Oxidations)
Edited by SIDNEY FLEISCHER DNA LESTER PACKER
EMULOV .WK Biomembranes (Part F: Bioenergetics)
Edited by SIDNEY FLEISCHER DNA LESTER PACKER