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Respiratory Cytology and Ancillary diagnostic techniques Dr Alex Rice Royal Brompton Hospital Overview • Specialist Cardiothoracic centre – BAL specimens and cell differential counts – EBUS • Diagnostic pitfalls • Subtyping lung cancer • Molecular techniques – Recent advances pleural fluids Bronchiolo-alveolar Lavage (BAL) specimens BAL Cytology • Indications (Acute or Chronic LD) – Interstitial and alveolar lung disease – Infections • E.g Pneumocystis, fungi, viral – Drug reactions – Malignancy • Techniques – Cell differential count – Fat laden macrophage – Immunofluorescence Lymphocytosis • ILDs associated with lymphocytosis include • Sarcoidosis • Hypersensitivity pneumonitis • NSIP/CTD • OP • Drug related • Infection: TB, viral pneumonia • Lymphoma Neutrophilia • ILDs associated with neutrophilia – IPF/UIP • Infection – Fungal stains – Correlate with microbiology • Vasculitis – Leukocytoclasis – haemosiderin laden macrophages Eosinophilia • Eosinophilic pneumonia • Asthma • ABPA • Drug reaction • Parasitic infection • Vasculitis (Churg Strauss) • Langerhans cell histiocytosis ITU – Acutely sick patient • Neutrophilia • +/- intracytoplasmic organisms (special stains) • Leukocytoclasis ->capillaritis? • Eosinophilia • Eosinophilic pneumonia/drug reaction/infection • Infectious agents • PCJ, fungi, CMV, HSV, TB • Gram, grocott, IHC, IF stains • Iron • Pulmonary haemorrhage syndrome/vasculitis • Reactive type 2 pneumocytes, debris, fibrin Reactive cytological atypia Post radiotherapy interstitial infiltrates Infection Pneumocystis

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Some studies 85% of cases classified as adeno or squamous using smears, cell pellet and immunohistochemistry. • NSCLC NOS. – Immuno: TTF-1

Respiratory Cytology and Ancillary diagnostic techniques PDF

79 Pages·2016·9.66 MB·English

by Alexandra Rice

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by Alexandra Rice| 2016| 79 pages| 9.66| English

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About Respiratory Cytology and Ancillary diagnostic techniques

Some studies 85% of cases classified as adeno or squamous using smears, cell pellet and immunohistochemistry. • NSCLC NOS. – Immuno: TTF-1

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Author:	Alexandra Rice
Publication Year:	2016
Pages:	79
Language:	English
File Size:	9.66
Format:	PDF
Price:	FREE

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