REDISTRIBUTION OF 5'-NUCLEOTIDASE IN CHOLESTATIC LIVER

10 Pages·1992·3.7 MB·

by  San-Duo Chen

Bull. Inst. Zool., Academia Sinica 31(4): 251-260 (1992)
REDISTRIBUTION OF S'·NUCLEOTIDASE IN CHOLESTATIC
LIVER
SAN-Duo CHEN
Department of Veterinary Medicine, National Chung-Hsing University,
Taichung, Taiwan, 40227 Republic of China
(Accepted January 20, 1992)
The mechanism behind the elevation of 5'-nucleotidase (5'-N) activity in cholestatic
serum was studied by immunocytochemistry. By using immunolabelling, it was observed
that 5'-N was transported from the bile canalicular membrane to the sinusoidal membrane
in bile duct ligated liver. The process was carried out by canalicular internalization, ves
iculation, intracellular vesicle migration, and fusing with the sinusoidalmembrane. There
was no evidence of misaddressing to the lateral membrane, and only very few cases
showed a rupture of the tight junction releasing bile into serum from the canalicular lu
men. In addition, some hepatic cells were necrosed after bile duct ligation. It is suggested
that the reverse transcytosis is the main route for bile regurgitation, although the leakage
of the tight junction and the necrosis of hepatocyte can not be ruled out. The elevation
of 5'-N activity in cholestatic serum is most likely caused by the release of 5'-N into the
blood stream from the sinusoidal membrane which had been enriched with 5'-N from the
canalicular membrane following bile duct ligation.
Key words: 5' -nucleotidase, Immunogold labelling, Reverse transcytosis.
has also been reported that 51-nucleotidase is
5'
-nucleotidase is an ectoenzyme which mostly concentrated in the bile canalicular
is widely distributed in ani~al cells (Trams, membrane, as compared with the basolateral
1974; De Pierre and Karnovsky, 1974; membrane (Meier et at., 1981; Sztul et al.,
Newby et ai., 1975; Farquhar et al., 1974; 1987).
Stanley et at., 1980, 1983). It spans the Via cytochemistry (Essner et aI, 1958;
plasma membrane bilayers (Zachowski et Desmet et ai., 1968; Farquhar et ai., 1974)
al., 1981), and its active site is expressed on and immunolabelling techniques (Stanley et
the external face of a cell. (De Pierre and ai., 1983; Geuze et ai., 1984; Matsuura et
Karnovski, 1974; Newby et al., 1975). Its at., 1984; Chen, 1990), it has been con
stability and ease of assay has made it a con cluded that 51-nucleotidase is distributed not
venient and popular marker of the cytoplas only in the bile canalicular and sinusoidal
mic membrane (Evans, 1976). membranes but also in the Golgi complex
The distribution of 51-nucleotidase in rat and endoplastic reticulum. However, Geuze
liver has been studied in detail. From cell et ai. (1984) localized this enzyme by im
fraction studies, it was found that 51-nucleo munogold labelling of ultracryosections, and
tidase exhibits a dual distribution-peaking in reported that 51-nucleotidase was not distrib
both the nuclear and microsomal fractions uted on the lateral membranes of hepato
(Beaufay et al., 1974; Draye et ai., 1987). It cytes. Chen (1990) also reported that 51-nu-
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