Reconstitution of Intracellular Transport

466 Pages·1992·12.106 MB·English

by  James E. Rothman (Eds.)

Contributors to Volume 219
elcitrA numbers are in parentheses following the names of contributors.
snoitailiffA listed are .tnerruc
STEPHEN A. ADAM (11), School of Medicine, School of Medicine, Stanford, California
Northwestern University, Chicago, Illinois 94305
60611 H. W. NOSDIVAD (12, 25), Departments of
W. E. HCLAB (12, 25), Departments of Cell Cell and Molecular Biology, Scripps Re-
and Molecular Biology, Scripps Research search Institute, La Jolla, California
Institute, La Jolla, California 92037 9203 7
NOC J. M. SREKCEB (2, 31), Section of In- NASUS FERRO-NovlcK (14), Department of
fectious Diseases, Department of Internal Cell Biology, Yale University School of
Medicine, Yale University School of Medi- Medicine, New Haven, Connecticut 06510
cine, New Haven, Connecticut 06510 RENIAR FRANK (37), European Molecular
MARY A. BITTNER (17), Department of Biology Laboratory, D-6900 Heidelberg,
Pharmacology, University of Michigan Germany
Medical School, Ann Arbor, Michigan
RETEID ZTiWLLAG (35), Department of Mo-
48109
lecular Genetics, Max-Planck-Institute of
MARC R. KCOLB (28), Laboratoire d'Etude Biophysical Chemistry, D-3400 ,negnittOG
des Systemes, Adhesifs Cellulaires, Germany
A.T.LP.E. du ANRS Associe~ d I'URA
ELLEHCIM D. TTERRAG (33), Department of
1178, OtisrevinU .J Fourier, F53X-38041
Cell Biology, Yale University School of
,elbonerG France
Medicine, New Haven, Connecticut 06510
MAILLIW A. LLEARB (3), Department of Bio-
YRRAL ECAREG (11), Departments of Cell
logical Chemistry and Molecular Pharma-
and Molecular Biology, Scripps Research
,ygoloc Harvard Medical School, Boston,
Institute, La Jolla, California 92037
Massachusetts 02115
KCIRTAP DLAWNNERB (33), Department of YUrdKO GODA (15), Molecular Neurobiol-
Cell Biology, Yale University School of ogy Laboratory, The Salk Institute, La
Medicine, New Haven, Connecticut 06510 Jolla, California 92037
EPPILIHP REIRVAHC (37), European Molecu- NEITSAB D. STREPMOG (18), Department of
lar Biology Laboratory, D-6900 Heidel- Physiology, University College London,
,greb Germany London WC1E 6J ,J England
DOUGLAS O. CLARY (30), Howard Hughes YRAM E. HCSEORG (14), Department of Cell
Medical Institute, University of ,ainrofilaC Biology, Yale University School of Medi-
San Francisco, San Francisco, California cine, New Haven, Connecticut 06510
94143 REGDOL HENGST (35), Department of Mo-
AIRAM LEBASI OBMOLOC (5), Department of lecular Genetics, Max-Planck-Institute of
Cell Biology and Physiology, Washington Biophysical Chemistry, D-3400 ,negnittOG
University School of Medicine, St. Louis, Germany
Missouri 63110, and Instituto de Histolo- ADNIL HICKE (32), Department of Biochem-
gia y Embriologia, Universidad Nacional istry, Biocenter, University of Basel, CH-
de Cuyo, 5500 Mendoza, Argentina 4056 Basel, Switzerland
ENERI ZALUEHT-Ys.~HTROC (16), Depart- DLANOR W. HOLZ (17), Department of
ment of Biochemistry, Stanford University Pharmacology, University of Michigan
ix
X CONTRIBUTORS TO VOLUME 219
Medical School, Ann Arbor, Michigan TREBOR NOTRAP (37), European Molecular
90184 Biology Laboratory, D-6900 ,grebledieH
DNALEIW B. HUTTNER (10), Institute for Germany
,ygoloiborueN University of ,grebledieH RUPERT PFALLER (8), Department of -loiB
D-6900 ,grebledieH Germany ogy, University of ,ainrofilaC San ,ogeiD
ASILA K. LLENECBAK (33), Department of La Jolla, ainrofilaC 39029
,yrtsimehcoiB Boehringer Ingelheim -rahP ENNAZUS R. PFEFFER (15, 16), Department
,slacituecam Inc., Ridgefield, tucitcennoC of Biochemistry, Stanford University
77860 School of Medicine, Stanford, ainrofilaC
DRAHCIR A. KAHN (34), Laboratory of Bio- 50349
logical Chemistry, Division of Cancer
S. PIND (12), Departments of Cell and Mo-
Treatment, National Cancer Institute, Na-
ralucel ,ygoloiB Scripps Research Institute,
tional Institutes of Health, Bethesda,
La ,alloJ ainrofilaC 92037
Maryland 29802
NALA PITT (4), Department of Cell Biology
SEMAJ M. DRAHNEL (5), Department of Cell
and Physiology, Washington University
Biology and Physiology, Washington -inU
School of Medicine, St. Louis, Missouri
versity School of Medicine, .tS Louis, Mis-
01136
souri 01136
H. RENTULP (12), Departments of Cell and
J, PAUL LuzIo (7), Department of lacinilC
raluceloM ,ygoloiB Scripps Research Insti-
Biochemistry, Addenbrooke's Hospital,
tute, La Jolla, ainrofilaC 73029
ytisrevinU of ,egdirbmaC Cambridge CB2
2QR, England NIMAJNEB ZCIWEILIBDOP (20), MRC Labo-
ratory of Molecular Biology, Cambridge
Luls S. AGROYAM (4, 5), Instituto de Histo-
CB2 2QH, England
aigol y Embriologia, Universidad Na-
cional de Cuyo, 5500 Mendoza, anitnegrA MARC TREAPYP (21), Cell Biology -arobaL
tory, Imperial Cancer Research Fund,
SEMAJ M. MclLVAIN, JR. (9), Department of
London WC2 3PX, England
Cell Biology, Duke University Medical
,retneC Durham, North aniloraC 01772 LUAP A. OZZADNAR (34), Laboratory of -oiB
ARI NAMLLEM (20), Department of Cell Bi- logical Chemistry, Division of Cancer
,ygolo Yale University School of ,enicideM ,tnemtaerT National Cancer Institute, Na-
New Haven, Connecticut 01560 tional Institutes of Health, Bethesda,
Maryland 29802
NEHPETS G. MILLER (23), Department of
Molecular and Cell ,ygoloiB University of SAMOHT E. REIEMLEDER (22), Departments
,ainrofilaC Berkeley, Berkeley, ainrofilaC of Cell and Molecular ,ygoloiB Scripps Re-
02749 search Institute, La Jolla, California
ONIP-OAISH H. EROOM (23), Department of 73029
Molecular and Cell ,ygoloiB University of LEAHCIM REXACH (26), Division of Bio-
,ainrofilaC Berkeley, ainrofilaC 02749 chemistry and Molecular ,ygoloiB Howard
ARABRAB M. KCOLLUM (7), Department of Hughes Medical Research Institute, Uni-
Clinical Biochemistry, Addenbrooke's versity of ,ainrofilaC Berkeley, Berkeley,
Hospital, University of ,egdirbmaC Cam- ainrofilaC 02749
bridge CB2 2QR, England ANILADNEUG ROSSI (14), Department of
JOHN W. NEWPORT (8), Department of -loiB Cell Biology, Yale University School of
ogy, University of ,ainrofilaC San ,ogeiD Medicine, New Haven, tucitcennoC 01560
La Jolla, ainrofilaC 39029 SEMAJ E. NAMHTOR (1, 2, 27, 28, 29, 30, 31),
RETEP KCIVON (33), Department of Cell Bi- yrotarobaL of Cellular Biochemistry,
,ygolo Yale University School of ,enicideM gniretteK-naolS Institute for Cancer Re-
New Haven, Connecticut 06510 ,hcraes New York, New York I0021
CONTRIBUTORS TO VOLUME 219 xi
YDNAR W. NAMKEHCS (13, 26, 32), Depart- DNAMRA TAVITIAN (36), Facult~ de M~de-
ment of Molecular and Cell Biology, How- cine Lariboisi~re-Saint Louis, INSERM
ard Hughes Medical Research Institute, U248, Paris 75010, France
University of California, Berkeley, Berke- NORAHS A. TOOZE (10), Department of Cell
ley, California 94720 Biology, European Molecular Biology
SANDRA L. SCHMID (22), Departments of Laboratory, D-6900 Heidelberg, Germany
Cell and Molecular Biology, Scripps Re- RETEP RENOAW (35), Department of Molec-
search Institute, La Jolla, California ular Genetics, Max-Planck-Institute of
92037 Biophysical Chemistry, D-3400 ,negnittOG
R. REGN1NAWHCS (12), Departments of Cell Germany
and Molecular Biology, Scripps Research MAHARG WARREN (21, 24), Cell Biology
Institute, La Jolla, California 92037 Laboratory, Imperial Cancer Research
ALAN L. ZTRAWHCS (4), Department of Fund, London WC2 3PX, England
Pharmacology and Pediatrics, Washington M. GERARD WATERS (31), Department of
University School of Medicine, St. Louis, Molecular Biology, Princeton University,
Missouri 63110 Princeton, New Jersey 08544
RUTH A. RETNES (17), Department of Phar- OFRA WEISS (34), Department of Endocri-
macology, University of Michigan Medical nology and Metabolism, Hadassah Uni-
School, Ann Arbor, Michigan 48109 versity Hospital, Jerusalem 91120, Israel
TITO SERAFINI (27), Department of Anat- FELIX T. WIELAND (19), Institutfftr Bioche-
omy, University of California, San Fran- mie I, Universitdt Heidelberg, D-6900
cisco, San Francisco, California 94143 Heidelberg, Germany
LEAHCIM P. ZTEEHS (9), tnemtrapeD of lleC NACNUD W. NOSLIW (29), MCR Laboratory
Biology, Duke University Medical Center, of Molecular Biology, University Postgrad-
Durham, North Carolina 27710 uate Medical School, Cambridge CB2
HTEBAZILE EHTYMS (22), Departments of 2QH, England
Cell and Molecular Biology, Scripps Re- PHILIP G. WOODMAN (24), Department of
search Institute, La Jolla, California Biochemistry and Molecular Biology, Uni-
9203 7 versity of Manchester Medical School,
YRREIHT ITADLOS (15), Department of Bio- Manchester MI 3 9PT, England
chemistry, Stanford University School of ADNIL J. WUESTEHUaE (13), Division of
Medicine, Stanford, California 94305 Biochemistry and Molecular Biology,
PHILIP D. LHATS (4, 5), Department of Cell Howard Hughes Medical Institute, Uni-
Biology and Physiology, Washington Uni- versity of California, Berkeley, Berkeley,
versity School of Medicine, St. Louis, Mis- California 94720
souri 63110 TOHRU ASIHIHSOY (32), Division of Bio-
LEHCAR RRAM-ENRETS (11), Jefferson Med- chemistry and Molecular Biology, Howard
ical College, Thomas Jefferson University, Hughes Medical Institute, University of
Philadelphia, Pennsylvania 19107 California, Berkeley, Berkeley, California
HTEBAZILE SZTUL (6), Department of Mo- 94720
lecular Biology, Lewis Thomas Labora- DEMHA IUOARHAZ (36), Facult~ de M~de-
tory, Princeton University, Princeton, New cine Lariboisi~re-Saint Louis, INSERM
Jersey 08544 U248, Paris 75010, France
RETEP E. R. TATHAM (18), Department of ONIRAM ZERIAL (37), European Molecular
Physiology, University College London, Biology Laboratory, D-6900 Heidelberg,
London WCIE 6J J, England Germany
Preface
This volume of Methods in Enzymology reflects both the breadth and
intensity of current activities toward accomplishing and dissecting cell-free
reconstitutions of numerous transport steps. Assembling this collection
was made easy by the willingness and enthusiasm of the many investigators
who so generously contributed their time and expertise, for which I am
most grateful.
SEMAJ E. ROTHMAN
×iii
METHODS IN ENZYMOLOGY
EMULOV I. Preparation and Assay of Enzymes
detidE yb YENDIS P. KCIWOLOC DNA NATHAN O. KAPLAN
EMULOV II. Preparation and Assay of Enzymes
detidE yb YENDIS P. KCIWOLOC DNA NATHAN O. NALPAK
EMULOV III. Preparation and Assay of Substrates
Edited yb YENDIS P. KCIWOLOC DNA NATHAN O. NALPAK
EMULOV IV. Special Techniques for the Enzymologist
detidE yb YENDIS P. KCIWOLOC DNA NATHAN O. NALPAK
EMULOV V. Preparation and Assay of Enzymes
detidE yb YENDIS P. KCIWOLOC DNA NATHAN O. KAPLAN
EMULOV VI. Preparation and Assay of Enzymes )deunitnoC(
Preparation and Assay of Substrates
Special Techniques
detidE yb YENDIS P. KCIWOLOC DNA NATHAN O. KAPLAN
EMULOV VII. Cumulative Subject Index
Edited yb YENDIS P. KCIWOLOC DNA NATHAN O. NALPAK
EMULOV VIII. Complex Carbohydrates
detidE yb HTEBAZILE F. NEUFELD DNA VICTOR GRUBSNIG
EMULOV IX. Carbohydrate Metabolism
Edited yb WILLIS A. WOOD
EMULOV X. Oxidation and Phosphorylation
detidE yb RONALD W. KOORBATSE DNA MAYNARD E. NAMLLUP
EMULOV XI. Enzyme Structure
detidE yb C. H. W. HIRS
EMULOV XII. Nucleic Acids (Parts A and B)
detidE yb ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XIII. Citric Acid Cycle
detidE yb J. M. NIETSNEWOL
EMULOV XIV. Lipids
detidE yb J. M. NIETSNEWOL
EMULOV XV. Steroids and Terpenoids
detidE yb DNOMYAR B. NOTYALC
EMULOV XVI. Fast Reactions
detidE yb KUSTIN KENNETH
VX
xvi SDOHTEM IN YGOLOMYZNE
EMULOV XVII. Metabolism of Amino Acids and Amines (Parts A and B)
detidE yb TREBREH ROBAT DNA WHITE CELIA ROBAT
EMULOV XVIII. Vitamins and Coenzymes (Parts A, B, and C)
detidE yb DONALD B. McCoRMICK DNA LEUMEL n. WRIGHT
EMULOV XIX. Proteolytic Enzymes
detidE yb GERTRUDE E. NNAMLREP DNA DNAROL OLZSAL
EMULOV XX. Nucleic Acids and Protein Synthesis (Part C)
detidE yb KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXI. Nucleic Acids (Part D)
detidE yb ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXII. Enzyme Purification and Related Techniques
detidE yb B. WILLIAM YBOKAJ
EMULOV XXIII. Photosynthesis (Part A)
detidE yb YNOHTNA NAS PIETRO
EMULOV XXIV. Photosynthesis and Nitrogen Fixation (Part B)
detidE yb ANTHONY NAS PIETRO
EMULOV XXV. Enzyme Structure (Part B)
detidE yb C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVI. Enzyme Structure (Part C)
detidE yb C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVII. Enzyme Structure (Part D)
detidE yb C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVIII. Complex Carbohydrates (Part B)
detidE yb VICTOR GRUBSNIG
EMULOV XXIX. Nucleic Acids and Protein Synthesis (Part E)
detidE yb ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXX. Nucleic Acids and Protein Synthesis (Part F)
detidE yb KtvlE EVADLOM ECNERWAL DNA NAMSSORG
EMULOV XXXI. Biomembranes (Part A)
detidE yb YENDIS REHCSIELF DNA LESTER REKCAP
EMULOV XXXII. Biomembranes (Part B)
detidE yb YENDIS REHCSIELF DNA RETSEL REKCAP
EMULOV XXXIII. Cumulative Subject Index Volumes I-XXX
detidE yb MARTHA G. DENNIS DNA DRAWDE A. SINNED
EMULOV XXXIV. Affinity Techniques (Enzyme Purification: Part B)
detidE yb B. WILLIAM YBOKAJ DNA MEIR WILCHEK
EMULOV XXXV. Lipids (Part B)
detidE yb JOHN M. NIETSNEWOL
METHODS IN ENZYMOLOGY xvii
EMULOV XXXVI. Hormone Action (Part A: Steroid Hormones)
Edited by BERT W. O'MALLEY DNA JOEL G. NAMDRAH
EMULOV XXXVII. Hormone Action (Part B: Peptide Hormones)
Edited by BERT W. O'MALLEY DNA JOEL G. NAMDRAH
EMULOV XXXVIII. Hormone Action (Part C: Cyclic Nucleotides)
Edited by JOEL G. NAMDRAH DNA BERT W. O'MALLEY
EMULOV XXXIX. Hormone Action (Part D: Isolated Cells, Tissues, and Organ
Systems)
Edited by JOEL G. NAMDRAH DNA BERT W. O'MALLEY
EMULOV XL. Hormone Action (Part E: Nuclear Structure and Function)
Edited by BERT W. O'MALLEY DNA JOEL G. NAMDRAH
EMULOV XLI. Carbohydrate Metabolism (Part B)
Edited by W. A. WOOD
EMULOV XLII. Carbohydrate Metabolism (Part C)
Edited by W. A. WOOD
EMULOV XLIII. Antibiotics
Edited by JOHN H. HASH
EMULOV XLIV. Immobilized Enzymes
Edited by KLAUS HCABSOM
EMULOV XLV. Proteolytic Enzymes (Part B)
Edited by OLZSAL DNAROL
EMULOV XLVI. Affinity Labeling
Edited by WILLIAM B. YBOKAJ DNA MEIR WILCHEK
EMULOV XLVII. Enzyme Structure (Part E)
Edited by C. H. W. Hms DNA EGRES N. EEEHSAMIT
EMULOV XLVIII. Enzyme Structure (Part F)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XLIX. Enzyme Structure (Part G)
Edited by C. H. W. HIRs DNA EGRES N. FFEHSAMIT
EMULOV L. Complex Carbohydrates (Part C)
Edited by VICTOR GRUBSNIG
EMULOV LI. Purine and Pyrimidine Nucleotide Metabolism
Edited by AICIRTAP A. HOFFEE DNA MARY ELLEN SENOJ
EMULOV LII. Biomembranes (Part C: Biological Oxidations)
Edited by YENDIS REHCSIELF DNA RETSEL REKCAP
EMULOV LIII. Biomembranes (Part D: Biological Oxidations)
Edited by YENDIS REHCSIELF DNA RETSEL REKCAP
EMULOV LIV. Biomembranes (Part E: Biological Oxidations)
Edited by YENDIS REHCSIELF DNA RETSEL REKCAP
xviii METHODS IN ENZYMOLOGY
EMULOV LV. Biomembranes (Part F: Bioenergetics)
detidE yb YENDIS REHCSIELF DNA LESTER REKCAP
EMULOV LVI. Biomembranes (Part G: Bioenergetics)
detidE yb YENDIS REHCSIELF DNA LESTER REKCAP
EMULOV LVII. Biolumineseence and Chemiluminescence
detidE yb ENELRAM A. D~LuCA
EMULOV LVIII. Cell Culture
detidE yb WILLIAM B. YBOKAJ DNA IRA NATSAP
EMULOV Eli. Nucleic Acids and Protein Synthesis (Part G)
detidE yb KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV LX. Nucleic Acids and Protein Synthesis (Part H)
detidE yb KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV 61. Enzyme Structure (Part H)
detidE yb C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV 62. Vitamins and Coenzymes (Part D)
detidE yb DONALD B. MCCORMICK DNA LEMUEL D. WRIGHT
EMULOV 63. Enzyme Kinetics and Mechanism (Part A: Initial Rate and Inhibitor
Methods)
detidE yb DANIEL L. PURICH
EMULOV 64. Enzyme Kinetics and Mechanism (Part B: Isotopic Probes and
Complex Enzyme Systems)
detidE yb DANIEL L. PURICH
EMULOV 65. Nucleic Acids (Part I)
detidE yb ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV 66. Vitamins and Coenzymes (Part E)
detidE yb DONALD B. MCCORMICK DNA LEMUEL D. WRIGHT
EMULOV 67. Vitamins and Coenzymes (Part F)
detidE yb DONALD B. MCCORMICK DNA LEUMEL n. WRIGHT
EMULOV 68. Recombinant DNA
detidE yb RAY Wu
EMULOV 69. Photosynthesis and Nitrogen Fixation (Part C)
detidE yb YNOHTNA NAS PIETRO
EMULOV 70. Immunochemical Techniques (Part A)
detidE yb HELEN VAN SIKANUV DNA JOHN J. ENOGNAL
EMULOV 71. Lipids (Part C)
detidE yb JOHN M. NIETSNEWOL
EMULOV 72. Lipids (Part D)
detidE yb JOHN M. NIETSNEWOL
SDOHTEM NI YGOLOMYZNE xix
EMULOV 73. Immunochemical Techniques (Part B)
Edited by JOHN J. ENOGNAL DNA HELEN NAV SIKANUV
EMULOV 74. Immunochemical Techniques (Part C)
Edited by JOHN J. LANGONE DNA HELEN VAN SIKANUV
EMULOV 75. Cumulative Subject Index Volumes XXXI, XXXII, XXXIV-LX
Edited by DRAWDE A. DENNIS DNA MARTHA G. SINNED
EMULOV 76. Hemoglobins
Edited by ODLARE ANTONINI, LUIGI ROSsI-BERNARDI, DNA EMILIA ENOCNAIHC
EMULOV 77. Detoxication and Drug Metabolism
Edited by WILLIAM B. YBOKAJ
EMULOV 78. Interferons (Part A)
Edited by YENDIS AKTSEP
EMULOV 79. Interferons (Part B)
Edited by YENDIS AKTSEP
EMULOV 80. Proteolytic Enzymes (Part C)
Edited by OLZSAL DNAROL
EMULOV 81. Biomembranes (Part H: Visual Pigments and Purple Membranes, I)
Edited by RETSEL REKCAP
EMULOV 82. Structural and Contractile Proteins (Part A: Extracellular Matrix)
Edited by LEON W. MAHGNINNUC DNA DIXIE W. NESKIREDERF
EMULOV 83. Complex Carbohydrates (Part D)
Edited by VICTOR GRUBSNIG
EMULOV 84. Immunochemical Techniques (Part D: Selected Immunoassays)
Edited by JOHN J. ENOGNAL DNA HELEN NAV SIKANUV
EMULOV 85. Structural and Contractile Proteins (Part B: The Contractile Appa-
ratus and the Cytoskeleton)
Edited by DIXIE W. NESKIREDERF DNA LEON W. MAHGNINNUC
EMULOV 86. Prostaglandins and Arachidonate Metabolites
Edited by WILLIAM E. M. SDNAL DNA WILLIAM L. SMITH
EMULOV 87. Enzyme Kinetics and Mechanism (Part C: Intermediates, Stereo-
chemistry, and Rate Studies)
Edited by DANIEL L. PURICH
EMULOV 88. Biomembranes (Part I: Visual Pigments and Purple Membranes, II)
Edited by RETSEL REKCAP
EMULOV 89. Carbohydrate Metabolism (Part D)
Edited by WILLIS A. WOOD
EMULOV 90. Carbohydrate Metabolism (Part E)
Edited by WILLIS A. WOOD
XX SDOHTEM IN YGOLOMYZNE
EMULOV 91, Enzyme Structure (Part I)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV 92. Immunochemical Techniques (Part E: Monoclonal Antibodies and
General Immunoassay Methods)
detidE yb JOHN J. LANGONE DNA HELEN VAN SIKANUV
EMULOV 93. Immunochemical Techniques (Part F: Conventional Antibodies, Fc
Receptors, and Cytotoxicity)
detidE yb JOHN J. LANGONE DNA HELEN VAN SIKANUV
EMULOV 94. Polyamines
Edited by TREBREH TABOR DNA CELIA WHITE TABOR
EMULOV 95. Cumulative Subject Index Volumes 16 -74, 76-80
Edited by DRAWDE A. SINNED DNA AHTRAM G. SINNED
EMULOV 96. Biomembranes Part J: Membrane Biogenesis: Assembly and Tar-
geting (General Methods; Eukaryotes)
detidE yb YENDIS REHCSIELF DNA BECCA REHCSIELF
EMULOV 97. Biomembranes Part K: Membrane Biogenesis: Assembly and Tar-
geting (Prokaryotes, Mitochondria, and Chloroplasts)
Edited by YENDIS REHCSIELF DNA BECCA REHCSIELF
EMULOV 98. Biomembranes (Part L: Membrane Biogenesis: Processing and Recy-
cling)
Edited by YENDIS REHCSIELF DNA BECCA REHCSIELF
EMULOV 99. Hormone Action (Part F: Protein Kinases)
Edited by EIKCAJ O. NIBROC DNA JOEL G. NAMDRAH
EMULOV 100. Recombinant DNA (Part B)
Edited by YAR Wu, ECNERWAL ,NAMSSORG DNA K/VIE EVADLOM
EMULOV 101. Recombinant DNA (Part C)
detidE yb RAY Wu, ECNERWAL ,NAMSSORG DNA K/VIE EVADLOM
EMULOV 102. Hormone Action (Part G: Calmodulin and Calcium-Binding Pro-
teins)
Edited by ANTHONY R. MEANS DNA BERT W. O'MALLEY
EMULOV 103. Hormone Action (Part H: Neuroendocrine Peptides)
Edited by P. LEAHCIM NNOC
EMULOV 104. Enzyme Purification and Related Techniques (Part C)
Edited by WILLIAM B. YBOKAJ
EMULOV 105. Oxygen Radicals in Biological Systems
Edited by RETSEL REKCAP
EMULOV 106. Posttranslational Modifications (Part A)
Edited by FINN WOLD DNA K/VIE EVADLOM
EMULOV 107. Posttranslational Modifications (Part B)
Edited by FINN WOLD DNA K/VIE EVADLOM