Receptor Purification: Volume 1 Receptors for CNS Agents, Growth Factors, Hormones, and Related Substances

497 Pages·1990·17.46 MB·English

by  Timothy K. Gallaher

Receptor Purification
Receptor
Purification
Volume 1
Receptors for CNS Agents,
Growth Factors, Hormones,
and Related Substances
Edited by
Gerald Litwack
Fels Institute
for Cancer Research and Molecular Biology,
Philadelphia, PA
~~
Springer Science+Business Media, LLC
Library of Congress Catalog ing in Publication Data
Receptor purification / edited by Gerald Litwack
p. cm.
Includes index.
Contents: v. 1. Receptors for CNS agents, pituitary growth
factors, hormones, and related substances.
ISBN 978-1-4612-6771-3 ISBN 978-1-4612-0461-9 (eBook)
DOI 10.1007/978-1-4612-0461-9
1. Neurotransmitter receptors-Purification. 2. Hormone
receptors-Purification. Cell receptors-Purification.
1. Li twack , Gerald.
[DNLM: 1. Receptors, Endogenous Substances-isolation &
purification. au 55 R2956]
OP364.7.R4278 1990
612.4-dc20
DLC
for Library of Congress 90-4689
CIP
© 1990 Springer Science+Business Media New York
Originally published by Humana Press Inc. in 1990
Softcover reprint of the hardcover 1s t edition 1990
AII rights reserved
No part of this book may be reproduced, stored in a retrieval
system, or transmitted in any form or by any means, electronic,
mechanical, photocopying, microfilming, recording or otherwise
without written permission from the Publisher.
Preface
The purpose of these volumes is to provide a reference work for the
methods of purifying many of the receptors we know about. This be
comes increasingly important as full-length receptors are overexpressed
in bacteria or in insect cell systems. A major problem for abundantly
expressed proteins will be their purification. In addition to purification
protocols, many other details can be found concerning an individual
receptor that may not be available in standard texts or monographs. No
book of this type is available as a compendium of purification procedures.
Receptor Purification provides protocols for the purification of a wide
variety of receptors. These include receptors that bind: neurotransmit
ters, polypeptide hormones, steroid hormones, and ligands for related
members of the steroid supergene family and others, including receptors
involved in bacterial motion. The text of this information is substantial,
so as to require its publication in two volumes. Consequently, a division
was made by grouping receptors by the nature of their ligands. Thus, in
Volume One there are contributions on serotonin receptors, adrenergic
receptors, the purification of GTP-binding proteins, opioid receptors,
neurotensin receptor, luteinizing hormone receptor, human chorionic
gonadotropin receptor, follicle stimulating hormone receptor, thyro
tropin receptor, prolactin receptor, epidermal growth factor receptor,
platelet derived growth factor receptor, colony stimulating factor recep
tor, insulin-like growth factor receptors, insulin receptor, fibronectin
receptor, interferon receptor, and the cholecystokinin receptor. In some
cases, for the better known receptors, more than one manuscript has been
sought and included in this collection because of the manifold approaches
to the purification of a given receptor. Representation of several ap
proaches could be beneficial and outweigh the costs of increased numbers
of pages. Also, for some receptors, purification is tailored for different
forms of the receptor.
Many of the contributions to these volumes are from laboratories
that lead the field not only in purification but in the phenomenology of
receptors, both at the protein level and at the molecular biological level.
v
vi Preface
Any gaps in coverage of the specific receptors usually result from difficul
ties in obtaining manuscripts from representative laboratories or gener
ate from contracts with laboratories that failed ultimately to provide a
manuscript. Nevertheless, the coverage of various receptor types is broad
and should be of considerable benefit to researchers in this overall area.
We now begin to witness the overexpression of full-length receptors
in bacterial or baculovirus systems. Overexpression solves an important
problem in receptor research in that it overcomes the difficulties of con
centration typified by many receptors that occur in the nanomolar range.
With the advent of overexpression systems it is now possible to generate
receptors in the micromolar range advancing their status from trace
proteins to abundant cellular proteins. These proteins need to be purified
from the cells overexpressing them. Such purification processes must
rely heavily on the information garnered for purification of the same
receptor from various cells and tissues. This information is surveyed in
this two volume work and appears at a time coinciding with the need to
purify receptors from overexpression systems. The presentation of in
formation about each receptor is complete enough so that all of the proto
cols required for the purification process are available without further
pursuit of the literature for precise details. Moreover, in some cases, es
pecially in Volume Two, there are contributions on specific agents useful
for covalent binding of ligands to their receptors. In many cases informa
tion on cloning is presented that may be helpful to investigators consid
ering overexpression systems. The material available in this two volume
work should be of great use to biologists, biochemists, pharmacologists,
molecular biologists, and endocrinologists, including graduate students
as well as sophistocated workers.
I thank the Publisher for responsIveness and rapid generation of
these volumes in a diligent manner. I am indebted to the contributors for
their conscientious preparation of manuscripts. I, and the contributors,
are further indebted to a wide variety of journals and other publications
that so freely allowed production of published data appearing in these
volumes. Denise Valentine helped in the preparation of the index and
other typing responsibilities.
Contents
Preface ........................................................................................ v
List of Contributors ............................................................... xi
Serotonin Receptors ................................................................. 1
Timothy K. Gallaher and Howard H. Wang
Purification and Cloning
of Central Serotonin Receptors .......................................... 23
Henri Gozlan, Salah EI Mestikawy,
and Michel Hamon
Purification of ~Adrenergic Receptors:
Isolation of Mammalian ~1- and ~2-Subtypes .................... 45
Craig C. Malbon
Purification of~, y Subunits
of Guanine Nucleotide Binding Proteins ........................... 77
Deirdre Cooney and Alan K. Keenan
Purification of Opioid Binding Proteins. ............................... 99
Theresa L. Gioannini and Eric J. Simon
J.l-Opioid Receptor: Purification and Reconstitution
with GTP-Binding Protein ............................................... 115
Hiroshi Ueda and Masamichi Satoh
The Neurotensin Receptor from Mammalian Brain:
Solubilization and Purification
by Affinity Chromatography ......................................... 131
Jean-Pierre Vincent, Jean Mazella, JoiUle Chabry,
and Nicole Zsurger
vii
viii Contents
The Luteinizing HormonelHuman Chorionic Gonadotropin
Receptor of Testis and Ovary:
Purification and Characterization ............................... 147
Maria L. Durau
Purification of LHlhCG Receptor ......................................... 173
Om P. Bahl and Hakimuddin T. Sojar
The Follicle Stimulating Hormone Receptor ....................... 191
Bosukonda Dattatreyamurty and Leo E. Reichert, Jr.
Thyrotropin Receptor:
Characterization and Purification ................................... 217
Takashi Akamizu, Michele De Luca,
and Leonard D. Kohn
Thyrotropin Receptor Purification. ..................................... 249
Peter J. Leedman and Leonard C. Harrison
Prolactin Receptors: Purification, Molecular Weight,
and Binding Specificity .................................................... 269
Arieh Gertler
Ligand- and Antibody-Affinity Purification
of the Epidermal Growth Factor Receptor. ...................... 289
George Panayotou, J. Justin Hsuan,
and Michael D. Waterfield
Platelet-Derived Growth Factor B Type Receptor. ............. 303
Carl-Henrik Heldin and Lars Ronnstrand
Colony-Stimulating Factor 1 Receptor ................................ 315
Yee-Guide Yeung and E. Richard Stanley
Insulin-Like Growth Factor-II Receptors ............................ 329
Robert C. Baxter and Carolyn D. Scott
Purification of Insulin
and Insulin-Like Growth Factor (IGF}-I Receptor .......... 347
Yoko Fujita-Yamaguchi and Thomas R. Le Bon
Contents ix
Insulin Receptor Isolation
Through Avidin-Biotin Technology. ................................. 373
Frances M. Finn and Klaus Hofmann
Insulin Receptor Purification. ............................................ .403
Julie D. Newman and Leonard C. Harrison
Insulin Receptor Purification. ............................................ .419
J. M. Maturo III and M. D. Hollenberg
Isolation of Fibronectin Receptors ....................................... 435
Kenneth M. Yamada and Susan S. Yamada
Fibronectin Receptor Purification
Using the Complementary Hydropathy Approach ......... .451
Ricardo Brentani and Renata Pasqualini
Purification of the Human Interferon-y Receptor
by Ligand Affinity ........................................................... 459
Daniela Novick, Dina G. Fischer,
and Menachem Rubinstein
Purification of the Cholecystokinin Receptor. .................... .483
Laurence J. Miller
Index. ................................................................................................ 497
Contributors
Takashi Akamizu • National Institutes of Health,
Bethesda, MD
Om P. Bahl • State University of New York, Buffalo, NY
Robert C. Baxter • Department of Endocrinology,
Royal Prince Alfred Hospital, Camperdown, Australia
Ricardo Brentani • Ludwig Institute
for Cancer Research, Sao Paulo, Brazil
JoiUle Chabry • Centre de Biochimie du Centre
National de la Recherche Scientifique, Nice, France
Deirdre Cooney • Department of Pharmacology,
University College, Dublin, Ireland
Bosukonda Dattatreyamurty • Department
of Chemistry, Albany Medical College, Albany, NY
Michele De Luca • Instituto Nazionale
per la Recerca suI cancro, Genoa, Italy
Maria L. Dufau • National Institutes of Health,
Bethesda, MD
Frances M. Finn • Department of Medicine, University
of Pittsburgh, Pittsburgh, PA
Dina G. Fischer • Department of Virology,
The Weizmann Institute of Science, Rehovot, Israel
Yoko Fujita-Yamaguchi • Department of Molecular
Genetics, Beckman Research Institute of the City of Hope,
Duarte, CA
Timothy J. Gallaher· Department of Biology,
University of California, Santa Cruz, CA
Arieh Gertler • Department of Biochemistry
and Human Nutrition, The Hebrew University of Jerusalem,
Rehovot, Israel
Theresa I.. Gioannjni • Department of Psychiatry,
New York University Medical Center, NY, NY
xi
xii Contributors
Henri J. Gozlan • INSERM, Paris, France
Michel Hamon • INSERM, Paris, France
Leonard C. Harrison • Walter and Eliza Hall Institute
of Medical Research, Royal Melbourne Hospital, Parkville,
Victoria, Australia
Carl-Henrik Heldin • Ludwig Institute for Cancer
Research, Uppsala, Sweden
M. D. Hollenberg • Department of Pharmacology
and Therapeutics, University of Calgary, Calgary, Alberta,
Canada
Klaus Hofmann • Department of Medicine, University
of Pittsburgh, Pittsburgh, PA
J. Justin Hsuan • Ludwig Institute for Cancer Research,
London, UK
Alan K. Keenan • Department of Pharmacology,
University College, Dublin, Ireland
Leonard D. Kohn • National Institutes of Health,
Bethesda, MD
Thomas R. LeBon • Department of Molecular Genetics,
Beckman Research Institute of the City of Hope,
Duarte, CA
Peter J. Leedman • Walter and Eliza Hall Institute
of Medical Research, Royal Melbourne Hospital, Parkville,
Victoria, Australia
Craig C. Malbon • Department of Pharmacology,
State University of New York, Stony Brook, NY
J. M. Maturo, III • C. W. Post College, Long Island
University, Greenvale, NY
Jean Mazalla • Centre de Biochimie du Centre
National de la Recherche Scientifique, Nice, France
Salah El Mestikawy • INSERM, Paris, France
Laurence J. Miller • Gastroenterology Unit,
Mayo Medical School and Graduate School of Medicine,
Rochester, MN
Julie D. Newman· Walter and Eliza Hall Institute
of Medical Research, Royal Melbourne Hospital, Parkville,
Victoria, Australia
Daniela Novick • Department of Virology,
The Weizmann Institute of Science, Rehovot, Israel