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About Ravisha Raina Archit Sharma Saaksha Mantoo Arushee Mantoo Dr. Anita Grewal
This document summarizes a research paper that optimized a method for extracting DNA from seven rose cultivars for molecular marker studies. The researchers modified a CTAB extraction procedure to improve DNA yield and quality by including high concentrations of NaCl, CTAB, beta-mercaptoethanol, and EDTA in the extraction buffer to remove polysaccharides and polyphenols. DNA was then purified using potassium acetate, chloroform, and isopropanol precipitation. Compared to a phenol-chloroform method, the modified CTAB procedure yielded higher quality DNA with less protein and polysaccharide impurities, as shown by agarose gel electrophoresis. The optimized extraction method provides high-quality DNA suitable for further molecular studies of rose plants
Detailed Information
| Author: | ['Novi Latifa'] |
|---|---|
| Publication Year: | 2015 |
| Pages: | 3 |
| Language: | English |
| Format: | |
| Price: | FREE |
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