Rajasthan University of Veterinary and Animal Sciences, Bikaner College of Veterinary and Animal ...

185 Pages·2016·4.01 MB·English

LIST OF ABBREVIATIONS

Abbreviation Meaning

D.W. Distilled water
DNA Deoxyribonucleic acid
dNTPs Deoxyribonucleotide triphosphate
EDTA Ethylene diamine tetra acetic acid
EtBr Ethidium bromide
IPTG Isopropyl – β- D- thiogalactoside
LB Luria- Bretani
LMP Low Melting Point
PBS Phosphate buffer saline
PCR Polymerase chain Reaction
PK Buffer Proteinase K Buffer
PK Enzyme Proteinase K Enzyme
R.T. Room temperature
RE Restriction enzyme
RoTat Rode Trypanozoon Antigen Type
RNase Ribonuclease
RPM Revolution per minute
SDS Sodium dodecyl sulphate
SOC Super Optimal Broath with Catabolite Repression
TAE Tris- Acetate EDTA
TbAT1 Trypanosoma brucei adenosine transporter 1
TevAT1 Trypanosoma evansi adenosine transporter 1
TE Tris EDTA
UV Ultra Violet
VSG Variable Surface Glycoprotein
X- gal 5- bromo-4-chloro- 3 –indolylβDgalatase
Units of Measurement

% Percentage
µg Microgram
µl Microlitre
0C Degree Celcius
A Absorbance
bp Base pair
cm Centimeter
Da Dalton
g Gram (s)
h Hour (s)
IU International unit
Kbp Kilo base pair
KDa Kilo Dalton
Kg Kilogram
M Molar
ma Milli ampere
mg Milli gram
min Minute (s)
Min Minute
ml Milli litre
mm Mili meter
mM Milli molar
ng Nano gram
OD Optical density
pmol Picomole
sec Second (s)
U Unit
V Volt
V / cm Volt per centimeter
V / V Volume / volume
W / V Weight / volume
Identification and Molecular Characterization of Variable Surface Glycoprotein and Adenosine
Transporter 1 Genes
of Trypanosoma evansi of Camel Origin

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REHAB ALI IBRAHIM YAGI

THESIS

DOCTOR OF PHILOSOPHY
(Veterinary Parasitology)
2013

Department of Veterinary Parasitology
College of Veterinary and Animal Science
Rajasthan University of Veterinary and Animal Sciences,
Bikaner – 334 001 (Rajasthan)
Identification and Molecular Characterization of Variable Surface Glycoprotein and Adenosine
Transporter 1 Genes
of Trypanosoma evansi of Camel Origin

Å¡V ls i`Fkd VªhiSukslksek bokUlh ds osjh;scy ljQsl XykbdksizksVhu vkSj ,Mhuksflu VªkaliksVZj 1 thu dh igpku vkSj
vkfUod vfHky{k.k

THESIS

Submitted to the
Rajasthan University of Veterinary and Animal Sciences, Bikaner
In partial fulfilment of the requirements for
the degree of

DOCTOR OF PHILOSOPHY
(Veterinary Parasitology)

By

REHAB ALI IBRAHIM YAGI

2013
Ph.D (Veterinary Parasitology) THESIS 2013 YAGI, R.A.I
Rajasthan University of Veterinary and Animal Sciences, Bikaner
College of Veterinary and Animal Science, Bikaner

CERTIFICATE - I

Date: ……………..

This is to certify that Rehab Ali Ibrahim Yagi has successfully completed the PRELIMINARY EXAMINATION held on 11-
06-2012 as required under the regulations for the degree of DOCTOR OF PHILOSOPHY.

( )
Dr. G. S. Manohar
61
Head,
Department of Veterinary Parasitology,
College of Veterinary and Animal Science, Bikaner.

62