Table Of ContentPSEUDOMONAS SYRINGAE PATHOV ARS AND RELATED PATHOGENS
Developments in Plant Pathology
VOLUME 9
The titles published in this series are listed at the end o/this volume.
Pseudomonas Syringae
Pathovars and Related
Pathogens
Edited by
K.RUDOLPH
Institut für Pflanzenpathologie und
Pflanzenschutz der Universität,
Göttingen, Germany
T.J.BURR
Department ofP lant Pathology,
NYSAES-Cornell University,
Geneva, NY, U.S.A.
J. W. MANSFIELD
Department Biological Sciences,
Wye College, University ofL ondon,
Wye, Ashford, Kent, U.K.
D.STEAD
Central Science Laboratory
Sand Hutton,
York, U.K.
A. VIVIAN
Department ofBiological Science,
UWE-Bristol, Frenchay,
Bristol, U.K.
J. VON KIETZELL
Biologische Bundesanstalt für
Land-und Forstwirtschaft,
Braunschweig, Germany
....
"
SPRINGER -SCIENCE+BUSINESS MEDIA, B.V.
A C.I.P. Catalogue record for this book is available from the Library of Congress
ISBN 978-94-010-6301-2 ISBN 978-94-011-5472-7 (eBook)
DOI 10.1007/978-94-011-5472-7
Printed on acid-free paper
All Rights Reserved
© 1997 Springer Science+Business Media Dordrecht
Originally published by Kluwer Academic Publishers in 1997
Softcover reprint ofthe hardcover 1st edition 1997
No part of the material protected by this copyright notice may be reproduced or
utilized in any form or by any means, electronic or mechanical,
including photocopying, recording or by any information storage and
retrieval system, without written permission from the copyright owner.
Contents
Contributors XIV
Preface XXV
K. Rudolph, TJ. Burr, J. W. Mansfield, D. Stead, A. Vivian
and J. von Kietzel/
Foreword XXVII
On the Track of Robert Koch in Berlin,
Klaus Naumann
Sponsors XXXIII
I: ECOLOGY AND EPIDEMIOLOGY
Attachment of Pseudomonas syringae to plant surfaces 3
Martin Romantschuk, Elina Roine and Katarina Bjorklof
Effect of soil moisture on the transmission of Pseudomonas syringae 11
pv. syringae from seed to seedling in pea
Krishan K. Jindal and Nandita Pathania
Modelling transmission of pea bacterial blight (Pseudomonas syringae 16
pv. pis/) from seed to seedling
Steven J. Roberts
Effect of host genotype and non-host plants on epiphytic life of 22
Pseudomonas syringae pv. pisi
Catherine Grondeau and Regine Samson
Bacteria from sweet pea (Pisum sativum L.) in the state of 26
Mexico, Mexico
Leopold Fucikovsky and Miguel Santos
Epiphytic occurrence of Pseudomonas syringae pv. atrofaciens 29
Jan von Kietzel/ and Klaus Rudolph
Qualitative aspects regarding the isolation of seed-borne 35
Pseudomonads in some varieties of Algerian wheat
Lamine Boukris and Nachat N. Nagha
Heterogeneity of the natural population of Pseudomonas syringae 40
pathovars
Lidia Pasichnik, Rostislav Gvozdyak and Svitlana Chodos
Bacterial and fungal diseases of lettuce (Lactuca sativa L.) in the 45
state of Mexico, Mexico
Leopold Fucikovsky and Silvia Ortega
Current status of Pseudomonas solanacearum research in Nepal 49
Tika Adhikari, Ramchandra Basnyat and Andrea Robinson-Smith
Effects of bacterial leaf spot on yield, resistance and seed borne 53
infection of sesame in Thailand
Sutruedee Prathuangwong and Petcharatana Yowabutra
v
vi
A pathovar of Pseudomonas syringae causal agent of bacterial leaf 61
spot and blight of pepper transplants
Momcilo Arsenijevic and Aleksa Obradovic
Orobanche ramosa as a new host for the epiphytic stage of 67
Pseudomonas syringae pv. tomato
Nevena Bogatzevska and Senka Deneva
Natural epiphytic survival of Pseudomonas syringae pv. phaseolicola 72
on weeds
Nevena Bogatzevska
Epiphytic survival of Pseudomonas syringae pv. glycine a on symptom- 77
less soybean and weeds
Nevena Bogatzevska
Pseudomonas syringae pv. syringae as microorganism involved in 82
apical necrosis of mango: Characterization of some virulence factors
Francisco M. Gazorla, Laura Olalla, Juan A. Tores, Juan A. Godina,
Alejandro Perez-Garcia and Antonio De Vicente
Influence of nitrogen fertilization on the colonization of olive phylloplane 88
by Pseudomonas syringae subsp. savastanoi
Giorgio M. Balestra and Leonardo Varvaro
Bacterial canker on apple, pear and cherry during recent years in 93
Germany.
Helmut Br6ther
Role of frost and stem water content on the development of bacterial 97
canker in acclimated stone fruit trees
Andre Vigouroux
Suppression of ice+ bacteria in suspensions and on corn seedlings 103
Horst Mittelstadt
II: THE RESISTANT REACTION OF THE PLANT 109
Imbalances in free radical metabolism: Roles in the induction of hyper- 111
sensitive response and local acquired resistance of plants
Attila L. Adam, Holger Deising, Balasz Barna, Gabor Gullner, Zoltan
Kiraly and Kurt Mendgen
HR-positive phenotype of the Pseudomonas syringae pv. syringae hrpK 122
mutant and hrp gene superinduction in tobacco leaves treated with
protein synthesis inhibitors
Zoltan Bozs6, Peter G. Ott and Zoltan Klement
The mechanism of symptomless reaction of plants induced by path 0- 127
genic pseudomonads
Zoltan Klement, Zoltan Bozs6, Peter G. Ott and Klaus Rudolph
Oleuropein and other polyphenols from olive (Olea europea) for protec- 133
ting the plant against Pseudomonas syringae subsp. savastanoi
Renato Capasso, Gennaro Cristinzio, Antonio Evidente, Carla
Visca and Gaterina lannini
Studies on the resistance of tomato cultivars against Pseudomonas 138
syringae pv. tomato races 0 and 1
Trimurti Habazar and Klaus Rudolph
vii
In planta labeling with biotin of macromolecules exposed on intercellular 144
surfaces of leaves
Bernd Sonnenberg, Roland Rohringer, Michael Gross and Klaus
Rudolph
Difference in suceptibility of plants to pathovars of Pseudomonas 150
syringae during the day
Rostis/av Gvozdyak, Lidia Pasichnik and Svitlana Chodos
Changes in the enzyme activities of Pisum sativum (garden pea) when 155
infected with Pseudomonas syringae pv. pisi
Claire B. Durant and Simon A. Archer
III: PATHOGENESIS 161
Genetic analysis of the role of toxin production by Pseudomonas 163
syringae pv. syringae in plant pathogenesis
Dennis C. Gross, Michael L. Hutchison, Brenda K. Scholz and
Jian-Hua Zhang
Lipodepsipeptides from Pseudomonas syringae pv. syringae 170
R. C. Bachmann, A. Ballio, D. Barra, F. Bossa, L. Camoni, D. Di
Giorgio, A. Di Nola, P. Ferranti, I. Grgurina, N.S. lacobellis, P.Laver-
micocca, C. Manetti, C. Marchiafava, G. Marino, K.A. Mott, M. Paci,
P. Pucci, A. Scaloni, A. Segre, M. Simmaco, G.A. Strobel, G.
Surico and J. Y. Takemoto
Comparison of biological activities of Pseudomonas syringae pv. 176
syringae toxins
Paola Lavermicocca, Nicola S. lacobellis and Maurizio Simmaco
Biosynthesis of bioactive lipodepsipeptides by Pseudomonas 182
syringae pv. syringae
Ingeborg Grgurina and Feliciana Mariotti
Detection of syringomycin in plant tissues infected with Pseudomonas 188
syringae pv. syringae
Ingeborg Grgurina, Nicola S. lacobellis, Ippolito Camele and
Raffaella Curci
SyrC, an enzyme involved in syringomycin biosynthesis, shows 192
thioesterasic activity
Ingeborg Grgrurina, Dennis C. Gross, loannis Deligiovas and
Jian-Hua Zhang
Studies on the effect of syringomycin and syringopeptins on the 198
functions of plant mitochondria
Paola Lavermicocca, Alessandro Ballio, Lorenzo Camoni,
Domenico Di Giorgio and Camilla Marchiafava
Isolation and characterization of mutants of Pseudomonas syringae 202
pv. syringae defective in production of syringopeptins
Arturo Caponero, Michael L. Hutchison, Nicola S. lacobellis and
Dennis C. Gross
A new syringopeptin produced by a Pseudomonas syringae strain from 208
Laurel
Domenico Di Giorgio, Lorenzo Camoni, Andrea Scaloni, Marco
Scortichini and Alessandro Ballio
viii
Syringomycins and syringopeptins in the basal glume rot of wheat 210
incited by Pseudomonas syringae pv. atrofaciens
Vassil Vassilev, Paola Lavermicocca, Domenico Di Giorgio and
Nicola lacobeflis
Biological effects of Pseudomonas fuscovaginae toxins on rice cells 215
Henri Batoko, Marie-Christine Flamand, Marc Boutry, Jean-Marie
Kinet and Henri Maraite
Production of phytotoxic lipodepsipeptides by Pseudomonas 221
fuscovaginae
Marie-Christine Flamand, Eric Ewbank, Bernard Goret and Henri
Maraite
Inhibition of rice (Oryza sativa L.) internode elongation by Pseudomonas 227
fuscovaginae toxins
Henri Batoko, Jules Bouharmont, Jean-Marie Kinet and Henri
Maraite
Thermo-regulation of coronatine biosynthesis in Pseudomonas syringae 230
Matthias Ullrich, Alejandro Penaloza-Vasquez, Ana-Maria Bailey and
Carol L. Bender
Role of light in the symptom development of bacterial speck in tomato 236
Alejandro Perez-Garcia, Francisco M. Canovas, Francisco R. Canton,
Francisco M. Cazorla, Marfa E. Rivera, and Antonio De Vicente
Cloning and physical characterization of the tox-argK gene clusters 242
from Pseudomonas syringae pv. phaseolicola, and phaseolotoxin
production in Escherichia coli
Efstathios Hatziloukas, Yuichi Takikawa, Norman Schaad and
Nickolas Panopoulos
Cloning of a putative peptide synthetase gene involved in the synthesis 248
of phaseolotoxin
Kuersad Turgay, Andre S. Bachmann, Mohamed Marahiel and
Suresh S. Patil
Influence of oxygen concentration on growth and phaseolotoxin 255
secretion of Pseudomonas syringae pv. phaseolicola
Heinrich Lehmann-Danzinger, Karin Jarchow-Redecker and
Klaus Rudolph
Pathochemical investigations on a phytotoxin secreted by Pseudomo- 261
nas syringae pv. syringae in bush bean leaves (Phaseolus vulgaris L.)
Islam Din Khan and Klaus Rudolph
Role of exopolysaccharides from Pseudomonas syringae pathovars in 265
pathogenesis
Klaus Rudolph and Bernd Sonnenberg
Structure of the extracellular polysaccharide of Pseudomonas syringae 271
pv. phaseolicola synthesized in sucrose containing media
Farouk EI-Banoby and Klaus Rudolph
Occurrence of levansucrase in phytopathogenic bacteria 277
Ursula Hettwer, Matthias N611enburg and Klaus Rudolph
Screening for the levansucrase-gene in Pseudomonas syringae 281
pv. phaseolicola
Manfred Meyer, Ursula Hettwer and Klaus Rudolph
ix
Exopolysaccharides produced by Pseudomonas syringae pathovars 282
Wagih EI-Shouny, Abd EI-Raheem EI-Shanshoury, Mostafa A.
EI-Sayed, Kerstin Wydra and Klaus Rudolph
Isolation and characterization of the algD gene of Pseudomonas 287
syringae pv. phaseolicola S2-1
Birger Koopmann, Matthias N61/enburg and Klaus Rudolph
Extracellular polysaccharide of Pseudomonas andropogonis (Smith) 293
Stapp induces water-congestion in millets
Islam Din Khan and Klaus Rudolph
Serotypic variability of Pseudomonas syringae pv. tabaci strains 297
Lyudmila Ya ko vIe va, Lidia Pasichnik, N.B. Porembskaya, Galina
Zdorovenko and Vassil Vassilev
Macromolecular characteristics and structure of the O-specific poly- 300
saccharide chain of Pseudomonas syringae pv. tomato O-antigen
Galina Zdorovenko, Yuriy Knirel, Lyudmila Solyanik, Lyudmila
Yakovleva and Vassil Vassilev
Interactions between phytopathogenic pseudomonads and plant cell 305
cultures
Jens MOl/er, Beate V61ksch and Wolfgang Fritsche
Auxin production by various pathovars of Pseudomonas syringae 311
Eric Glickmann, Louis Gardan, Miena Elasri and Yves Dessaux
Ethylene production by Pseudomonas syringae pathovars 317
Helge Weingart and Beate V61ksch
IV: DETERMINANTS OF PATHOGENICITY 323
Extracellular proteins as determinants of pathogenicity in Pseudomonas 325
syringae
Alan Col/mer, David W. Bauer, James R. Alfano, Gail Preston,
Amy O. Loneiel/o and Timothy M. Milos
Production of a harpin elicitor by Pseudomonas syringae pv. syringae 333
isolated from a nectarine tree
Maryke Appel, Lucienne E. Mansvelt and Dirk U. Bel/stedt
Characterization of a hrp· mutant from an olive-strain of Pseudomonas 339
syringae subsp. savastanoi
Angelo Sisto, Maria Morea, Filomena Zaccaro, Gioacchino Palumbo
and Nicola S. lacobellis
Pseudomonas syringae pv. aptata HRP mutants which fail to produce 343
periplasmic oligosaccharides
Paola Minardi and Emilio Stefani
Surface proteins as markers of Pseudomonas syringae pathovars 348
Laurence Malandrin and Regine Samson
The potential use of monospecific antibodies against Pseudomonas 352
syringae pathovars to elucidate host parasite interactions
Frank Niepold
The lectin activity of Pseudomonas syringae pathovars 358
Nataliya Kucheryava and Svitlana Votselko
Rheological interactions between lipopolysaccharides of Pseudomonas 364
syringae pathovars and plant polysaccharides
Ute Grolms and Klaus Rudolph
x
Cloning and characterization of pectate lyase genes from 370
Pseudomonas marginalis
Rangasamy P. Eluma/ai and Ayyamperumal Mahadevan
Exo-polygalacturonase and virulence in Pseudomonas so/anacearum 376
Caitilyn Allen, Oi Huang and Yaowei Kang
V: GENETIC ANALYSIS OF HOST/PLANT-INTERACTION 383
Analysis of gene-for-gene interactions between Pseudomonas syringae 385
pv. phaseolico/a and Phaseolus
John Mansfield, George Tsiamis, Nakul Puri, Mark A. Bennet, Carol
Jenner, Conrad Stevens, Dawn Teverson, N. Lyons and John Taylor
Expression of the Pseudomonas syringae pv. phaseolico/a hrpZ gene 392
in transgenic tobacco and Saccharomyces cerevisiae
Natassa Tabakaki, Efstathios Hatziloukas, Charlotte Grimm and
Nickolas J.Panopoulos
Preliminary characterization of the hrp gene cluster of Pseudomonas 397
syringae pv. pisi
Robert Jackson, Dawn Arnold, Benoit Cournoyer and Alan Vivian
Pseudomonas syringae hrp genes: regulation and role in the 403
phenotypic expression of avr genes
Steven Hutcheson, Minna Pirhonen, Daniel Rowley, Michael Udell,
Songmu Jin, Seon Woo Lee and Noel Keen
The molecular basis of specificity in the interaction between Pseudo- 408
monas syringae pv. pisi and cultivars of Pisum sativum
Alan Vivian, Benoit Cournoyer, Marjorie Gibbon and John Taylor
Hybridization of the hrpZ gene from Pseudomonas syringae pv. 413
syringae strain 61 with DNA of other bacteria and the polymerase
chain reaction-based amplification of this region
Lucienne E. Mansvelt and Maryke Appel
The genes involved in glyphosate utilization by Pseudomonas 417
pseudomallei and the tolerance conferred to plants
Alejandro Penaloza-Vazquez, Gilda L. Mena, Aracely Oropeza and
Ana M. Bailey
VI: IDENTIFICATION AND TAXONOMY 425
Modern methods for classification of plant pathogenic bacteria including 427
Pseudomonas syringae
David E. Stead, Judy Hennessy, John G. Elphinstone and Judith K.
Wilson
Review of Plant Bacterial diseases caused by fluorescent Pseudomo- 435
nas species in Mexico
Leopold Fucikovsky
DNA relatedness among pathovars of P. syringae and related bacteria 445
Louis Gardan, Hussain Shafif and Patrick A.D. Grimont
Detection of Pseudomonas syringae pv. phaseolico/a in agroeco- 449
systems using Bio-PCR
Norman W. Schaad, Efstathios Hatziloukas and Nickolas
Panopoulos
