Table Of ContentRachhpal S. Kahlon Editor
Pseudomonas:
Molecular
and Applied
Biology
Pseudomonas
: Molecular and Applied
Biology
ThiSisaFMBlankPage
Rachhpal S. Kahlon
Editor
Pseudomonas
: Molecular
and Applied Biology
Editor
RachhpalS.Kahlon
DepartmentofMicrobiology
PunjabAgriculturalUniversity
Ludhiana,Punjab
India
ISBN978-3-319-31197-5 ISBN978-3-319-31198-2 (eBook)
DOI10.1007/978-3-319-31198-2
LibraryofCongressControlNumber:2016940046
#SpringerInternationalPublishingSwitzerland2016
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Fondly dedicated to Iqbal Kahlon
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Preface
Inthe early 1970s, when Ijoined the bandwagon ofPseudomonasasapostdoc at
theTechnicalUniversityofDenmark,Copenhagen,Pseudomonaswasconsidered
asavaguelydefinedgenuscomprisingsoil-bornesaprophytes,attimesassociated
with wound infection. However, with the developments in molecular biology,
geneticengineering,PCR,DNA,andgenomesequencingsupportedbytechnologi-
cal developments and computer-based databases, Pseudomonas has attained the
position of a model organism for in silico modeling and systems biology where
largechunksofunwantedDNAsegmentcanbereplacedbydesiredones.Presently,
itisanimportantorganismforbiotechnologicalproductionofnovelchemicalsand
pharmaceuticalsaswellasforscientificpursuitsforexploringnewareasofitsvast
activities.
This volume was planned with a view to provide concise and comprehensive
information to young graduates, teachers, and research workers to visualize the
potentialofthisimportantanddiversegroup.
ThefirstchapteronPhylogenyandMolecularTaxonomyhas been contributed
by distinguished scientists, Elena Garcia-Valdes and Jorge Lalucat, who discuss
howthetaxonomyofPseudomonashasundergoneachangeandthemembersthat
were not true Pseudomonas were assigned to other subclasses of Proteobacteria.
Pseudomonas aeruginosa an opportunistic pathogen is notorious for hospital-
acquired infection, multiple drug resistance, and a cause of CF of lungs.Quorum
sensing and the mechanism of pathogenesis and virulence have been discussed.
Two chapters on cell envelope and metabolic network highlight the structure and
functionof cell envelope, particularly the outer membrane andthe vast metabolic
potential of Pseudomonas. Mono- and di-oxygenases are the key enzymes for
degradation of recalcitrant molecules, and a chapter on these has been included.
One chapter is dedicated to genome and comparative genomics of important
species. Also a chapter on “in silico” modeling and use of bioinformatics and
databases has been included. Two chapters on applied biology elaborate on com-
mercialexploitationforproductionofnovelmoleculesandbiopolymers.Theother
highlightstheirroleindegradationoforganicchemicalpollutantsandbioremedia-
tion.ThelasttwodealwithPseudomonas–plantinteractions:oneexclusivelydeals
with plant pathogenesis by Pseudomonas syringae and the other elaborates their
roleonbiocontrolofplantdiseasesanddirectandindirectplantgrowthpromotion.
vii
viii Preface
Iwouldliketoplace onrecord myappreciationandthankstoAlena andJorge
who were the first to accept my request to contribute a chapter on phylogeny and
taxonomy.Thisactedasacatalystandwasasourceofencouragement.Iamequally
grateful to Drs Levin, Parveen Sharma, Nagina, Swaranjit, Prince Sharma, and
Saini and their colleagues for their outstanding contributions and making this
volumepossible.
I owe a great deal to my family, particularly my wife, Sukhdeep, for her
all-round support and help. I do hope to compensate my lovely grandchildren
AbhayandAmiebygivingthemmoretimenow.
FinancialsupportbywayofgrantfromtheDepartmentofScienceandTechnol-
ogy,GovernmentofIndia,NewDelhi,isacknowledgedwiththanks.Iamgrateful
toPrincipal,SCDGovtCollege,Ludhiana,andProfDrBMSarwar,Head,Deptof
Botany,forimplementationandsmoothfunctioningoftheprojectattheiresteemed
institute.
Lastly, I am grateful to my mentors, students, friends, and relatives who have
beenpartofthisbeautifuljourney.TimelyhelpofMr.GurdeepSinghforcomputer
typingisacknowledgedwiththanks.
Ludhiana,Punjab,India RachhpalS.Kahlon
December14,2015
Contents
1 Pseudomonas:MolecularPhylogenyandCurrentTaxonomy. . . . . 1
ElenaGarc´ıa-Valde´sandJorgeLalucat
2 CellEnvelope:MolecularArchitectureandFunction. . . . . . . . . . . 25
RachhpalS.Kahlon
3 Pseudomonas:TheVersatileandAdaptiveMetabolicNetwork. . . 81
PartapBirSingh,HarvinderSinghSaini,andRachhpalS.Kahlon
4 Pseudomonas:GenomeandComparativeGenomics. . . . . . . . . . . . 127
RachhpalS.Kahlon
5 PseudomonasOxygenases:NatureandFunction. . . . . . . . . . . . . . . 193
AbhaShukla,BrijdeepSingh,SwaranjitSinghCameotra,
andRachhpalS.Kahlon
6 QuorumSensinginPseudomonasaeruginosa:Mechanismand
RegulationofVirulence. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 231
SajalSarabhai,AmanjotKaur,NeenaCapalash,andPrinceSharma
7 InSilicoComparativeAnalysisofTypeVISecretionSystemsin
PseudomonasputidaLS46. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 257
ParveenKumarSharma,JilagamazhiFu,RichardSparling,
andDavidBernardLevin
8 PseudomonasforIndustrialBiotechnology. . . . . . . . . . . . . . . . . . . 281
RachhpalS.Kahlon
9 BiodegradationandBioremediationofOrganicChemicalPollutants
byPseudomonas. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 343
RachhpalS.Kahlon
ix
