PROTEIN DOWNSTREAM PROCESSING design, development and application of high.

508 Pages·2020·12.729 MB·English

Methods in
Molecular Biology 2178
Nikolaos E. Labrou Editor
Protein
Downstream
Processing
Design, Development, and Application
of High and Low-Resolution Methods
Second Edition
M M B
ETHODS IN OLECULAR IO LO GY
SeriesEditor
JohnM.Walker
School of Lifeand MedicalSciences
University ofHertfordshire
Hatfield, Hertfordshire, UK
Forfurther volumes:
http://www.springer.com/series/7651
For over 35 years, biological scientists have come to rely on the research protocols and
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indexedinPubMed.
Protein Downstream Processing
Design, Development, and Application of High
and Low-Resolution Methods
Second Edition
Edited by
Nikolaos E. Labrou
Laboratory of Enzyme Technology, Department of Biotechnology, School of Applied Biology
and Biotechnology, Agricultural University of Athens, Athens, Greece
Editor
NikolaosE.Labrou
LaboratoryofEnzymeTechnology
DepartmentofBiotechnology
SchoolofAppliedBiology
andBiotechnology
AgriculturalUniversityofAthens
Athens,Greece
ISSN1064-3745 ISSN1940-6029 (electronic)
MethodsinMolecularBiology
ISBN978-1-0716-0774-9 ISBN978-1-0716-0775-6 (eBook)
https://doi.org/10.1007/978-1-0716-0775-6
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Preface
Proteinsarethemostdiversegroupofbiomoleculesthatarevitalforcellularstructureand
function. The technological advances in the omics area and the efforts in proteomics
researchhaveincreasedtherateofdiscoveringmanynewproteinswithunknownstructure
andfunction.Thesenewlydiscoveredproteinspresentenormousopportunitiesforresearch
andindustrialapplication.Thekeyfactor for theircommercialexploitationdependsonthe
development ofan efficientand effective purificationprocedure. However, with thousands
of proteins—each displaying unique characteristics—the development of purification stra-
tegiesthatdelivertherequiredpurityneededfordownstreamapplicationsisimportant.The
challenge,therefore,istoseparatetheproteinofinterestfromalloftheothercomponentsin
the cell, especially the unwanted contaminating proteins, with reasonable efficiency, speed,
and yield, while retaining the biological activity and chemical integrity of the polypeptide.
Theincreasingrequirementfortheproductionofpureproteinsisforcingscientiststogaina
thorough understanding of protein purification methods and gain abilities and knowledge
toimprovecurrentanddevelopnewandmoreeffectivepurificationmethodsandprotocols.
This volume, which is the second edition of Vol. 1129 (2014), is designed to give the
laboratoryworkertheinformationneededtodesignandimplementasuccessfulpurification
strategy.Itpresentsreliableandrobustprotocolsinaconciseform,emphasizingthecritical
aspects on practical problems and questions encountered at the lab bench. Written in the
highlysuccessfulMethodsinMolecularBiologyseriesformat,eachchapterprovidesintro-
ductory material with an overview of the topic of interest; a description of methods,
materials, and reagents; readily reproducible step-by-step protocols, a Notes section for
tips on troubleshooting; and a collection of published data with an extensive list of refer-
encesfor furtherdetails.
This volume consists of thirty chapters. It is divided in five parts (I–V), each of which
dealingwithdifferentapproachesandmethods.PartI startswithanoverviewofscreening
anddesignofpurificationstrategiesandcoversinitialaspectsonhigh-throughputscreening,
methodsdevelopment,andmediaselection.PartsIIandIIIofthisvolumeconcentrateon
low-andhigh-resolutionproteinpurificationmethodsthatcurrentlyenjoyfrequentcitation
intheliteraturewiththeemphasisbeingonaffinitychromatography.Informationonscale-
upconsiderationsisgivenwhereappropriate.Asidefrommethodsrelateddirectlytopurifi-
cation, this volume includes a description of analytical techniques of value in protein
preparation.Forexample,muchspacehasbeenallowedinPartIVoncutting-edgeanalytical
techniques of purified proteins. The last section (Part V) presents a few selected examples
andcasestudies.
It is impossible for a single book volume to cover all of the different methods, techni-
ques, and applications of protein purification in which scientists have made significant
progress. Thus, I have selected key examples covering a wide range of diverge scientific
disciplinesandstate-of-the-artexperimentalapproaches,inordertoprovidethereaderwith
arepresentativesampleofcurrentstatusofthefield.
The present book would definitely be an ideal source of scientific information to
advanced students, junior researchers, and scientists involved in health sciences, cellular
andmolecularbiology,biochemistry,biotechnology,andotherrelatedareasinbothacade-
miaandindustry.
v
vi Preface
Isincerelyhopethatthereaderwillenjoytheinformationprovidedinthisbookandfind
its contents interesting and scientifically stimulating. I also hope that I have established a
successful compilation of chapters within the exciting area of protein purification. I would
like to thank all contributing authors for their enthusiasm and for the time they spent
preparingthechaptersfor thisbook.Iwouldalsoliketo thankDr.JohnWalker,theseries
editor, for putting forward the idea of the book and for his help and encouragement, and
everybodyatSpringer for theirhelpfuladvice,support,andprofessionalism.Withouttheir
cooperation,thisvolumewouldhavenotseenthelight.Lastbutnotleast,Iwouldespecially
like to thank my family for their understanding and patience during the editing and
organizationofthechapters.
Athens,Greece NikolaosE.Labrou
Contents
Preface ..................................................................... v
Contributors................................................................. xi
PART I SCREENING AND DESIGN PURIFICATION STRATEGIES
1 ProteinPurificationTechnologies......................................... 3
NikolaosE.Labrou
2 High-ThroughputProcessDevelopment:I—ProcessChromatography........ 11
AnuragS.RathoreandR.Bhambure
3 High-ThroughputProcessDevelopment:II—MembraneChromatography.... 21
AnuragS.RathoreandS.Muthukumar
4 MediaSelectioninIonExchangeChromatographyinaSingleMicroplate ..... 27
CharlotteCabanneandXavierSantarelli
5 High-ThroughputScreeningofDye-LigandsforChromatography ........... 35
SunilKumarandNarayanS.Punekar
6 TechnicalOptimizationfor theHigh-ThroughputPurification
ofAntibodiesonAutomatedLiquidHandlers.............................. 49
PeterM.Schmidt
PART II LOW-RESOLUTION PROTEIN PURIFICATION METHODS
7 AqueousTwo-PhaseSystemsforCleanupandRecovery
ofEnzymesfromPlantsandPlant-DerivedExtracts......................... 65
OscarAguilar,ErickHeredia-Olea,EstherPerez-Carrillo,
andMarcoRito-Palomares
8 AqueousTwo-Phase-AssistedPrecipitationofProteins:APlatform
forIsolationofProcess-RelatedImpuritiesfromTherapeuticProteins......... 81
AnuragS.RathoreandR.Bhambure
9 RecombinantProteinsCo-ExpressedandCo-PurifiedinthePresence
ofAntibodyFragments .................................................. 93
AriodeMarco
PART III HIGH-RESOLUTION PROTEIN PURIFICATION METHODS
10 AffinityTagsinProteinPurificationandPeptideEnrichment:
AnOverview........................................................... 107
AnaSofiaPina,I´risL.Batalha,AnaM.G.C.Dias,
andAnaCecı´liaA.Roque
11 SyntheticLigandAffinityChromatographyPurificationofHumanSerum
AlbuminandRelatedFusionProteins ..................................... 133
SharonWilliams,PhilMorton,andDevBaines
vii
viii Contents
12 Z :APurificationTagforSelectiveIon-ExchangeRecovery............... 149
basic
MyHedhammar,JohanNilvebrant,andSophiaHober
13 AnOrthogonalFusionTagforEfficientProteinPurification................. 159
˚
JohanNilvebrant,MikaelAstrand,andSophiaHober
14 BiomimeticAffinityLigandsforProteinPurification ........................ 167
ˆ
IsabelT.SousaandM.AngelaTaipa
15 SynthesisandEvaluationofDye-LigandAffinityAdsorbents
forProteinPurification.................................................. 201
EvangeliaG.Chronopoulou,GeorgiosPremetis,ChristinaVarotsou,
NikolaosGeorgakis,ElisavetIoannou,andNikolaosE.Labrou
16 DesignofAffinityChromatographyPeptideLigandsThrough
CombinatorialPeptideLibraryScreening.................................. 217
G.R.Barredo,S.L.Saavedra,M.C.Martı´nez-Ceron,
S.L.Giudicessi,M.M.Marani,F.Albericio,O.Cascone,
andS.A.Camperi
17 Z :AProteinA-DerivedDomainwithCalcium-Dependent
Ca
AffinityforMildAntibodyPurification .................................... 245
JuliaScheffel,SaraKanje,andSophiaHober
18 MacroporousPolymerMonolithsforAffinityChromatography
andSolid-PhaseEnzymeProcessing....................................... 251
E.G.Korzhikova-Vlakh,G.A.Platonova,andT.B.Tennikova
19 SampleDisplacementBatchChromatographyofProteins.................... 285
LauraHeikaus,SitiHidayah,ManasiaGaikwad,MartaKotasinska,
VerenaRichter,MarcelKwiatkowski,andHartmutSchlu¨ter
20 LectinAffinityChromatography:AnEfficientMethod
toPurifyHorseIgG3 ................................................... 301
Salvatore G.De-SimoneandDavidW.ProvanceJr.
21 Heparin-BindingAffinityTag:ANovelAffinityTagforSimple
andEfficientPurificationofRecombinantProteins.......................... 311
SanhitaMaity,MusaabAl-Ameer,RaviKumarGundampati,Shilpi
Agrawal,andThallapuranamKrishnaswamySureshKumar
22 ExpressionandPurificationofRecombinantProteinsinEscherichia
coliTaggedwiththeMetal-BindingProteinsSmbPandCusF3H+............ 329
JessicaJ.Gomez-Lugo,BryanD.Santos,DavidA.Perez-Perez,JorgeM.
Montfort-Gardeazabal,MeganM.McEvoy,andXristoZarate
23 AntibodyAggregateRemovalUsingaMixed-ModeChromatography
Resin.................................................................. 345
TaoChen,GailiGuo,GuoqingTan,YingWang,andYifengLi
PART IV ASSESSING PROTEIN STRUCTURAL INTEGRITY, PURITY,
AND STABILIZATION
24 ProteomicAnalysisofFoodAllergensbyMALDITOF/TOF
MassSpectrometry...................................................... 357
CosimaD.Calvano,MariachiaraBianco,IlarioLosito,
andTommasoR.I.Cataldi
Contents ix
25 ProteinStructureAnalysisandValidationwithX-RayCrystallography......... 377
AnastassiosC.Papageorgiou,NirmalPoudel,andJesseMattsson
26 MeasuringBindingConstantsofHis-TaggedProteinsUsingAffinity
ChromatographyandNi-NTAImmobilizedEnzymes....................... 405
AnnetteC.Moser,BenjaminWhite,andFrankA.Kovacs
27 StabilizationofProteinsbyFreeze-DryinginthePresenceofTrehalose:
ACaseStudyofTubulin................................................. 417
PavelDra´ber,VadymSulimenko,TetyanaSulimenko,andEduarda
Dra´berova´
PART V APPLICATIONS/CASE STUDIES
28 G-Protein-CoupledReceptorExpressionandPurification ................... 439
KarolinaCorin,LottaT.Tegler,andSotiriosKoutsopoulos
29 (Hyper)ThermophilicEnzymes:ProductionandPurification................. 469
PierpaoloFalcicchio,MarkLevisson,Serve´W.M.Kengen,Sotirios
Koutsopoulos,andJohnvanderOost
30 ScreeningofRecombinantLignocellulolyticEnzymesThrough
RapidPlateAssays ...................................................... 479
AnthiKarnaouri,AnastasiaZerva,PaulChristakopoulos,
andEvangelosTopakas
Index ...................................................................... 505