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PROPERTIES OP BOOTS SISRA IS
ietpanoscma aw isi infbchok
by
Sfiohael George lyseako
A Dissertation Submitted to the
ftnutuate Faoulty is Partial Fulfillment of
The Requirements for the Degree of
DOGIQR OP PHILOSOPHT ^
Major Subject* Protoaoology
Approved*
Iowa State College
1950
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UMI Number: DP12241
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Gs, L B 4
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CABSI 01* e«3M ©
Pag#
...................
mmoDvcnm*..,. 1
m m OP LITERATURE........................................................ 3
K B iia m M im # . ................................. 15
® e a e r a l . . * ......... 15
Sodium Salicylate end Ablastio Plasma... 18
Proteins and Ablastio Serum. .................................. 22
Tryptophan and Sodium Salioylate Activity. ............ 26
Pantothenic Acid and Ablastio Serum. ...................... 27
Glucose Levels and Ablastio Blood ......... SO
SISULTS AID DISCISSIOI.. ............... 52
Sodium Salioylate and Ablastio Plasma.*......... 32
Proteins and Ablastio Serum..*......................... 40
TPyptophan and Sodium Salioylate Activity. ................... 50
Pantotbenie Aoid and Ablastio Serum............................... 55
Glucose Levels and Ablastio B lo o d .............................. 55
SWMAtr AID COICLUSKMS ...................................... 86
LITERATURE CITED,..................................................... 72
ACMOWLBHMSflS............................................................ 78
T 9 5 0 9
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H I,
IX8T OP mBLSS
Table Pag*
1* Percentage of division forms in rat*
injected with (1) sodium salicylate
infected rat plasm, (2) i n n t plasm,
and (3) s a lin e ........................................... 34
2. Percentage of division forms in rats
Injected with (1) liaaune plasm treated
with aodium salioylate, (2) immune plasma,
and (3) salin e...,............................................... 36
3* Percentage of division forms in rats in
jected with dialysed plasm from sodium
salicylate treated and non-treated infected
rats* ........... 38
4* Summary of statistics comparing serum
protein levels among (1) T, lewial infected
rats, (2) sodium salioylaFe irealed Infected
rats, (3) sodium salicylate treated, and
(4) normal ra ts .........................• • • • • • • • 42
5. Bleetrophoretie analysis of pooled samples
of sera from (1) salioylate treated
infeoted rata, (2) T. lewial infeoted
rats, end (3) normal ralsTTT..,................. 4?
6. Percentage of division forms and protein
levels in rats infected with T. lewis1,
receiving salioylate and plamm, "andf of
controls .......... 49
7. Percentage of division form of
trypanosomes in rats receiving
(1) tryptophan plus sodium salicylate,
(2) sodium salicylate only, and
(3) untreated controls*. «... 52
8. Summary of statistics on pantothenic acid
assays of sera of rats with (l) T. lew!si
and salicylate, (2) J . lewisi, (1?) salicyl
ate, and (4) of normal r a ts ..................... 36
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ir.
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9. Summary of means in Experiment 9 ................... 88
10. Summary of statistics of blood
gluoos© doterminations on rats with
(1) T. lewial and salioylate, (2) f.
lewisi, T( j) sal1 ey late, and (4) of"*
normal r a ts .........................* ...... €0
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LIST OP FiaUBBS
Figure Page
1* Electrophoretic patterns of pooled
•era of normal rats (top), rats with
lewisi (middle), and rats with
ft I'ewisT and sodium salicylate
TreaBnF (bottom)*..................................... 45
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INHOBUGHOI
Shore has been described in Ifryganosoaa lewlsl infeetions in
rats an issmme reaetion that operates in two wayst first, an anti-
reproductive antibody is formed whieh, inhibits reproduction of
the parasites} then, a second antibody is formed whieh destroys
the trypanosomas. Ihltaferro (1932a) sailed the first antibody
ablastin and the second the trypanocidal antibody.
She question of the existence, formation, and operation of
ablastin has received a great deal of attention by many investi
gators. S&liaferro (1924, 1925, 1926, 1932a) reported studies on
the properties of ablastint he said that it was a protein in the
euglobulin fraction of senna, it was passively transferable, it
would not unite with trypanosomas in vitro, and that it neither
killed the parasites nor affected their motility or vitality.
Its only manifestation seemed to be that of In vivo suppression of
reproduction.
Blood sugar studies by Sohern (1928) and Poindexter (19S8)
indicated an inverse relation between the amount of sugar and
mmfcer of trypanosomas in pathogenic trypanosomiases• Linton
(1929) found that in the non-pathogenie jg. lewlai there was no
change in the blood sugar during the course of the infection.
In the laboratories at Iowa State College, Booker and
co-workers (1943, 194?) reported that deficiencies in
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pantothenic acid affected the antireproductive activity of ablastin
so that reproduction continued and the infection was pathogenic.
Sector and S&llagher (1947) found that treatment of infected rate
with sodium salicylate had a similar effect on ablastio activity*
Minoru (1940) reported changes in blood sugar levels in
rabbits as a result of sodium salicylate administration* In low
amounts them was a hypoglycemia* In greater amounts there was
hyporglyoeaia*
Brown (IfIS) and Taliaferro (1926) suggested that the differ
ence between pathogenic and con-pathogenic trypanosomas might be
based on the absence or presence of an antireproduotive factor in
the reaction of the host.
The relationships among pathogenicity, entireproductive
activity, glucose utilisation tod glucose levels, pantothenic acid
deficiency, and sodium salicylate treatment suggested that further
studies be made on immune sera with respect to these various
factors*
In this investigation, therefore, are reported studies on
immune sera of rats both treated with sodium salioylate and not
treated with respect to (1) ablastio activity, (2) changes in
protein levels, (S) pantothenate levels and (4) blood glucose
levels*
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