Process development of propionic acid production by fermentation

133 Pages·2016·2.28 MB·English

by  Zhong Gu

Iowa State University Capstones, Theses and
Retrospective Theses and Dissertations
Dissertations
1997
Process development of propionic acid production
by fermentation
Zhong Gu
Iowa State University
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Recommended Citation
Gu, Zhong, "Process development of propionic acid production by fermentation " (1997).Retrospective Theses and Dissertations.
11799.
https://lib.dr.iastate.edu/rtd/11799
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Process development of propionic acid production by fermentation
by
Zhong Gu
A dissertation submitted to the graduate faculty
in partial fulfillment of the requirements for the degree of
DOCTOR OF PHILOSOPHY
Major: Chemical Engineering
Major Professor: Charles E. Glatz
Iowa State University
Ames, Iowa
1997
DMI Number: 9737713
UMI Microform 9737713
Copyright 1997, by UMI Company. All rights reserved.
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ii
Graduate College
Iowa State University
This is to certify that the Doctoral dissertation of
Zhong Gu
has met the dissertation requirements of Iowa State University
Signature was redacted for privacy.
Major Professor
Signature was redacted for privacy.
For the Major Program
Signature was redacted for privacy.
oixme Graduate College
iii
I dedicate this work to my late father Yongkang.
iv
TABLE OF CONTENTS
ABSTRACT viii
INTRODUCTION I
Dissertation Organization 1
Literature Review 1
Propionic acid fermentation 1
Acid recovery from fermentation 3
Liquid-liquid extraction with amine 6
The solvent system 6
Extraction chemistry 7
Factors affecting amine extraction 10
Effect of pH 10
Effect of identity of acid 11
Effect of amine concentration 11
Effect of temperature 11
Acid regeneration 12
Solvent toxicity 13
Propionic acid downstream processes 16
PROPIONIC ACID PRODUCTION BY EXTRACTIVE FERMENTATION: PART 1.
SOLVENT CONSIDERATIONS 18
Summary 18
Introduction 18
V
Materials and Methods 22
Materials 22
Organic chemicals 22
Microorganism and media 22
Methods 23
Partitioning 23
Solvent toxicity 24
Strain screening 24
Single-component solvents 25
Reducing solvent toxicity 26
Distillation 26
Results 27
Partitioning 27
Solvent toxicity 30
Strain screening 30
Smgle-component solvent 33
Reducing solvent toxicity 33
Distillation 39
Discussion 39
Partitioning 39
Solvent toxicity 41
vi
Distillation 42
Conclusions 43
Acknowledgments 44
References 44
PROPIONIC ACID PRODUCTION BY EXTRACTIVE FERMENTATION: PART 2.
FERMENTATION STUDIES AND ECONOMIC EVALUATION 49
Summary 49
Introduction 50
Materials and Methods 53
Materials 53
Methods 53
Fermentation 53
Economic evaluation 56
Results 62
Fermentation 62
Economic evaluation 67
Discussion 72
Fermentation 72
Economic evaluation 78
Conclusions 79
Acknowledgments 80
References 80