Proceedings of the 34th Annual Aquatic Toxicity Workshop

293 Pages·2008·4.13 MB·English

th
Proceedings of the 34 Annual Aquatic Toxicity
Workshop: September 30 to October 3, 2007,
Halifax, Nova Scotia

ième
Compes rendus du 34 atelier annuel sur la
toxicité aquatique: du 30 Septembre au 3 Octobre
2007, Halifax, Nouvelle-Écosse

Editors/Éditeurs
K. A. Kidd1, R. Allen Jarvis2, K. Haya3, K. Doe4
and/et L.E. Burridge3

1University of New Brunswick Saint John,
Saint John, NB, E2L 4L5
2Environment Canada, Dartmouth NS, B2Y 2N6;
3Fisheries and Oceans Canada, St. Andrews, NB, E5B 2L9;
4Environment Canada, Moncton, NB, E1A 6SA

2008

Canadian Technical Report of Fisheries and
Aquatic Sciences 2793

Rapport Technique Canadien des Sciences
Halieutiques et Aquatiques 2793

Canada
Fisheries and Oceans Pêches et Océans
Canada Canada
Canadian Technical Report of
Fisheries and Aquatic Sciences

Technical reports contain scientific and technical information that contributes to existing
knowledge but which is not normally appropriate for primary literature. Technical reports
are directed primarily toward a worldwide audience and have an international distribution.
No restriction is placed on subject matter and the series reflects the broad interests and
policies of the Department of Fisheries and Oceans, namely, fisheries and aquatic sciences.
Technical reports may be cited as full publications. The correct citation appears above the
abstract of each report. Each report is abstracted in Aquatic Sciences and Fisheries
Abstracts and indexed in the Department’s annual index to scientific and technical
publications.
Numbers 1-456 in this series were issued as Technical Reports of the Fisheries Research
Board of Canada. Numbers 457-714 were issued as Department of the Environment,
Fisheries and Marine Service, Research and Development Directorate Technical Reports.
Numbers 715-924 were issued as Department of Fisheries and the Environment, Fisheries
and Marine Service Technical Reports. The current series name was changed with report
number 925.
Technical reports are produced regionally but are numbered nationally. Requests for
individual reports will be filled by the issuing establishment listed on the front cover and
title page. Out-of-stock reports will be supplied for a fee by commercial agents.

Rapport technique canadien des
sciences halieutiques et aquatiques

Les rapports techniques contiennent des renseignements scientifiques et techni-ques qui
constituent une contribution aux connaissances actuelles, mais qui ne sont pas normalement
appropriés pour la publication dans un journal scientifique. Les rapports techniques sont
destinés essentiellement à un public international et ils sont distribués à cet échelon. Il n’y a
aucune restriction quant au sujet; de fait, la série reflète la vaste gamme des intérêts et des
politiques du ministère des Pêches et des Océans, c’est-à-dire les sciences halieutiques et
aquatiques.
Les rapports techniques peuvent être cités comme des publications complètes. Le titre
exact parait au-dessus du résumé de chaque rapport. Les rapports techniques sont résumé
dans la revue Résumés des sciences aquatiques et halieutiques, et ils sont classés dans
l’index annual des publications scientifiques et techniques du Ministère.
Les numéros 1 à 456 de cette série ont été publiés à titre de rapports techniques de l’Office
des recherches sur les pêcheries du Canada. Les numéros 457 à 714 sont parus à titre de
rapports techniques de la Direction générale de la recherche et du développe-ment, Service
des pêches et de la mer, ministère de l’Environnement. Les numéros 715 à 924 ont été
publiés à titre de rapports techniques du Service des pêches et de la mer, ministère des
Pêches et de l’Environnement. Le nom actuel de la série a été établi lors de la parution du
numéro 925.
Les rapports techniques sont produits à l’échelon régional, mais numérotés à l’échelon
national. Les demandes de rapports seront satisfaites par l’établissement auteur dont le nom
figure sur la couverture et la page du titre. Les rapports épuisés seront fournis contre
rétribution par des agents commerciaux.
i
Canadian Technical Report of
Fisheries and Aquatic Sciences

2008

Proceedings of the 34thAnnual Aquatic Toxicity Workshop: September 30 to
October 3, 2007, Halifax, Nova Scotia
Compes rendus du 34ième atelier annuel sur la toxicité aquatique: du 30
Septembre au 3 Octobre 2007, Halifax, Nouvelle-Écosse

Editors/Éditeurs
K. A. Kidd1, R. Allen Jarvis2, K. Haya3, K. Doe4
and/et L.E. Burridge3
1University of New Brunswick Saint John,
Saint John, NB, E2L 4L5
2Environment Canada, Dartmouth NS, B2Y 2N6;
3Fisheries and Oceans Canada, St. Andrews, NB, E5B 2L9;
4Environment Canada, Moncton, NB, E1A 6SA

This is the two hundred and seventy ninth Technical Report
of the Biological Station, St. Andrews, NB
ii

© Her Majesty the Queen in Right of Canada 2008,
Cat. No. Fs97-6/2793E ISSN 0706-6457

Correct citation for this publication:

Kidd, K.A., Allen Jarvis, R., Haya, K., Doe, K., and Burridge, L.E. (Editors). 2008
Proceedings of the 34th Annual Aquatic Toxicity Workshop: September 30 to
October 3, 2007, Halifax, Nova Scotia. Can. Tech. Rep. Fish. Aquat. Sci. 2793:
xviii + 273p.
iii
Preface/Preface 

The 34th Annual Aquatic Toxicity Workshop was held at the Westin Nova Scotian,
Halifax, Nova Scotia September 30 to October 3, 2007. The Workshop included two
plenary presentations, 156 platform, 89 poster papers and several interactive sessions.
Total attendance was 397.
This workshop was one of a continuing series of annual workshops in Canada on aquatic
and environmental toxicology, covering topics from basic aquatic toxicology to
applications in environmental monitoring, setting of regulations and guidelines, and the
development of sediment and water quality criteria. These workshops emphasize an
informal exchange of ideas and knowledge on the topics among interested persons from
industry, governments and universities. They provide an annual focus on the principles,
current problems and approaches in aquatic toxicology. These workshops are
administered by a Board of Directors, and organized by local organizing committees.
The Proceedings are published with the support of the Department of Fisheries and
Oceans.

L’ 34ième atelier annuel sur la toxicité a eu lieu au Westin Nova Scotian à Halifax,
Nouvelle-Écosse, du 30 Septembre au 3 October 2007. Le atelier a donné lieu a 2
communication lors de séances plénières, 156 exposés d’invités d’honneur, 89
communications par affichage et plusieurs sessions interactives. 397 personnes ont
assisté au atelier.

Le atelier a premis de poursuivre les discussions tenues annuellement au Canada sur la
toxicologie aquatique et l’écotoxicologie. Ces atelier annuels organisés par un comité
national constitué légalement réunissent des représentants des secteurs industriels, des
administrations et des universités que le domaine intéresse. Ces derniers y échangent des
idées et des connaissances sur les notions fondamentales de la toxicologie aquatique,
mais aussi sur son application pour la surveillance de l’environnement, l’élaboration de
lignes directrices et de règlements, et la définition de critère pour les sédiments et pour la
qualité de l’eau. Ils passent également en revue les principes de la spécialité, de même
que les questions d’actualité et les méthodes adoptées dans le domaine. Les comptes
rendus sont publies l’aide du ministre des Pêches et Océans.
iv

Editors comments/Remarques des editeurs 

This volume contains papers, abstracts or extended abstracts of all presentations at the
workshop. And author index and list of participants are also included. The papers and
abstract were subject to limited review by the editors but were not subjected to full formal
or external review. In most cases the papers are published as presented and therefore are
of various lengths and formats. Comments on any aspects of individual contributions
should be directed to the authors. Any statements or views presented here are totally
those of the speakers and are neither condoned nor rejected by the editors. Mention of
trade names or commercial products does not constitute endorsement or recommendation
for use.

Ces comptes rendus sont publiés en deux volumes, en raison de leur longeur, ils
renferment le texte intégral ou le résumé de toutes les communications présentées aux
ateliers. Un index des auteurs et une liste des participants sont aussi inclus. Les
communications et les résumés ont été revus sommairement par les éditeurs, mais ils
n’ont pas fait l’objet d’une revue exhaustive en bonne et due forme ou d’une revue
indépendante. La lonueur et la forme des communications varient parce que ces
derniéres sont pour la plupart publiées intégralement. On est prié de communiquer
directement avec les auteurs pour faire des remarques sur le travaux. Toutes les
déclarations et opinions paraissant dans le présent rapport sont celles des conférenciers;
elle ne sont ni approuvées, ni rejetées par les éditeurs. La mention de marques de
commerce ou de produits commercialiesés ne constitue ni une approbation, ni une
recommandation d’emploi.
v
34th Aquatic Toxicity Workshop Organizing  
Committee/Comité d’organisation 

Workshop Co-Chairs:
Karen Kidd University of New Brunswick / Canadian Rivers Institute
Rosalie Allen Jarvis Environment Canada

Workshop organizing committee:
Kenneth Doe Environment Canada
Kats Haya Fisheries and Oceans Canada
Glenn Benoy Environment Canada / Agriculture and Agri-Food Canada
Charlene Burnett University of Saskatchewan
Jocelyne Hellou Fisheries and Oceans Canada
Mary Murdoch Jacques Whitford Ltd.
Simon Courtenay Fisheries and Oceans Canada
Mike van den Heuvel University of Prince Edward Island
Carolyn Hedley Intrinsik Environmental Sciences Inc.
Christine Moore Intrinsik Environmental Sciences Inc.
Tim Jardine University of New Brunswick/Canadian Rivers Institute
Rita Mroz Environment Canada
Les Rutherford Environment Canada
Kelly Munkittrick University of New Brunswick / Canadian Rivers Institute
Virginia Soehl Jacques Whitford Ltd.
Peter Wells Oceans One
vi
ATW Sponsors

Environment Canada
Fisheries and Oceans Canada
EnCana Corporation
Irving Oil
Jacques Whitford
Ontario Ministry of Environment
Agriculture and Agri-Foods Canada
Cantest
Canadian Copper & Brass Development Association
CVRD Inco
Nova Scotia Department of Environment and Labour
Pollutech EnviroQuatics
Stantec
Teck Cominco
Triton Environmental Consultants
ALS Environmental
ASI Group
C. Portt and Associates
Campbell Scientific
Canadian Rivers Institute
ExxonMobil
Hatfield Consultants
Intrinsik Environmental Sciences (formerly Cantox Environmental)
J.D. Irving
Minnow Environmental
Nautilus Environmental
Paine, Ledge and Associates
Sarnia-Lambton Environmental Association
Summit Environmental Consultants
University of Prince Edward Island
vii
Table of Contents/Tables des Metiére

Plenary
Navigating new waters in aquatic toxicology: The challenges of assessing and protecting the
health of aquatic ecosystems in Canada. Peter G. Wells 1
Winners of Dr. Richard Playle Awards
Real-time RT-PCR analysis of PPARα and acat1 expression in zebrafish: early development
profile and fibrate regulation. H. Parnas (PL) 27
Spatial and seasonal patterns of mercury bioaccumulation in yellow perch from the St.
Lawrence River at Cornwall, Ontario. A. Fowlie (PL) 28
Biological Test Methods Development and their Application
Trends in Method Development: is the LC50 test the dinosaur of toxicology? L. Taylor, L. Van
der Vliet and R. Scroggins (PL) 29
Assessment of methods for Cnidarian early life stage toxicity tests and immunotoxicology
analyses with a model hydrozoan, Hydractinia echinata. M. Nipper, R. Carr, J. T.
Lisle, K. Strychar, J. Moore and M. Grohmann (PL) 29
Environment Canada's echinoid fertilization test: The need for change. J. Miller, C. Buday, K.
Doe, P. Jackman, D. McLeay and R. Scroggins (PL) 30
Summary of method development research results for the echinoid embryo/larval sediment-
contact test. C. Buday, K. Doe, P. Jackman, R. Scroggins and G. Van Aggelen (PL) 31
Assessment of contaminant bioavailability and environmental quality of soils using a multi-
marker approach. P. Robidoux, V. Bérubé and G. Sunahara (PL) 31
Biological testing of soil using boreal forest plants. M. Moody, J. Princz and R. Scroggins
(PL) 32
New territory: Toxicity test methods to assess contaminated boreal forest soil. L. Van der
Vliet, J. Princz, M. Moody and R. Scroggins (PL) 32
Biomonitoring the safety of aquatic environments with fish cell lines. L. Lee, K.
Schirmer and N. C. Bols (PL) 33
Toxicity at the nanoscale level in trout hepatocytes exposed to quantum dots- exploring a new
realm of toxicity. F. Gagné, D. Maysinger and C. André (PL) 34
Increasing the efficiency of fathead minnow reproduction: the effect of relative size on
breeding potential. M. Pollock, F. Shelly1, C. Rickwood, A. Squires, D. Chivers and M.
Dubé (PL) 34
Effects of the PAH retene on rainbow trout (Oncorhynchus mykiss) immune response. N.
Hogan, K. Lee, B. Kollner and M. van den Heuvel (PL) 35
Do killifish from the Sydney Tar Ponds, Nova Scotia, Canada, overexpress P-glycoprotein? S.
Paetzold and S. Bard (PL) 36
A critical review of the Environment Canada Lemna minor Biological Test Method EPS
1/RM/37 – March 1999. D. Huebert (PL) 36
An investigative tool: Use of the Bacteroides Bacterial Source Tracking (BST) method to
identify fecal pollution sources in sediment, biota and aqueous samples (fresh, estuarine
or marine). M. Linssen Sauvé and H. Osachoff (PL) 37
The power of shared responsibility: Enabling community action to address land-based
pollution of Atlantic Canada’s coastal waters. M. Janowicz, P. Wells, I.
Novaczek and D. Tremblay (PL) 37
Lumbriculus variegates, a model for aquatic contaminants: Pharmaceuticals. J. Balon, N.
Tytka and P. Dehn (PO) 51
Parental dietary effect on echinoid progeny sensitivity to toxicants. R. Nelson, M. Nipper, A. L.
Lawrence and S. A. Watts (PO) 52
viii
Effects of hexabromocyclododecane (HBCD) diastereoisomers on the thyroid axis of juvenile
rainbow trout (Oncorhynchus mykiss). V. Palace, K. Wautier, K. Pleskach, M. Alaee, C.
Marvin and G. Tomy (PO) 52
The dirt on bait lamina and other proposed end points to assess soil microbial toxicity. J.
Rahn, L. Beaudette and R. Scroggins (PO) 53
Development of quantitative histopathology tools to assess instantaneous pressure-change-
induced effects in early life stages of rainbow trout (Onchorhynchus mykiss). V.
Palace, L. Peters, P. Cott, K. Wautier, R. Evans, B. Hanna and D. Godard (PO) 54
Induction of EROD activity in fish fed with benthic prey collected in the St. Lawrence beluga
whale habitat. C. M. Couillard, B. Légaré, É. Pelletier and M. G. Ikonomou (PO) 54
What’s an IGETG and is it contagious? L. Taylor, P. Jackman and J. Bruno (PO) 55
Nano in nanotechnology – Does size matter? S. Chan-Remillard, L. Kapustka, and S. Goudey
(PO) 56
Superfund sites and the development and application of ecological soil screening levels (Eco-
SSLs) for chemicals of concern. S. Goudey, L. Kapustka, L. Oosterbroek and A.
Hawkins (PO) 56
The effect of artificial sea salt preparation on Strongylocentrotus purpuratus fertilisation
success. J. Pickard, C. Sullivan and L. Stavroff (PO) 57
Quality control and method development: Some considerations in sediment toxicity testing. J.
Van Geest, K. Hunter, T. Watson-Leung and P. Jackman (PO) 57
Evaluation of nanoparticle toxicity using fish cell lines. M. Bufalino, J. Beitz, B. Peitrobon, K.
Hartlen, V. Kitaev and L. E. Lee (PO) 58
Development of a method to detect and quantify commercial microbial products (CMPs) using
quantitative polymerase chain reaction (qPCR). K. Bull, J. Hill , M. Douville and F.
Gagné (PO) 58
Oil and Gas Development and Production
Growth and biochemical responses of juvenile cod to acute and chronic exposure to
production water from the Hibernia oil rig. L. Burridge, M. Lyons, T. Blair, K.
Haya and K. Lee (PL) 59
Effect of produced water on cod (Gadus morhua) immune responses. D. Hamoutene, G.
Mabrouk, S. Samuelson, H. Volkoff, C. Parrish, A. Mansour, A. Mathieu and K. Lee
(PL) 59
Offshore Environmental Effects Monitoring programs on the Newfoundland Grand Banks: The
Terra Nova and White Rose programs. E. DeBlois, M. Paine, G. Janes, F. Wight, D.
Taylor, E. Tracy and U. Williams (PL) 60
Effects of offshore oil drilling on benthic invertebrate communities on the Grand Banks (North
Atlantic). E. DeBlois, F. Wight, D. Taylor and M. Paine (PL) 61
The "physical toxicity" side of oil and gas: Are seismic surveys an important risk factor for fish
and shellfish? C. D. Andrews, J. F. Payne, L. L. Fancey, J. Hanlon, A. Cook, J. R.
Christian and M. L. Rise (PL) 61
Wave tank studies on chemical dispersant effectiveness as a function of wave: Implication in
spatial and temporal toxicity response. Z. Li, K. Lee, B. C. Michel and A. D. Venosa
(PL) 62
Alkyl phenanthrenes, fluorenes, and napthobenzothiophenes are the main components of crude
oil that are chronically toxic to fish. P. Hodson, C. Khan, G. Saravanabhavan, L.
Clarke, S. Brown, B. Hollebone, Z. Wang, J. Short, T. King and K. Lee (PL) 62
Carbon dynamics, food web structure and reclamation strategies in Athabasca oil sands
wetlands (CFRAW). J. J. Ciborowski, G. Dixon, L. Foote, K. Liber and J. E. Smits (PL) 63
Application of PETROTOX in chemical risk assessments of petroleum stream substances. M.
Lopez, R. McWhinney and T. Stieglitz (PL) 64