Table Of Content

Contents Page: xi Preface Page: ix 1. Overview of bioseparations engineering Page: 1 1.1. Introduction Page: 1 1.2. What is separated in bioseparation? Page: 2 1.3. Economic importance of bioseparation Page: 3 1.4. Nature of bioseparation Page: 4 1.5. Basis of separation in bioseparation processes Page: 5 1.6. Physical forms separated in bioseparation Page: 6 1.7. Bioseparation techniques Page: 8 1.8. The RIPP scheme Page: 9 1.9. Example of bioseparation Page: 10 1.10. Current trends in the bioseparation Page: 10 2. Properties of biological material Page: 13 2.1. Introduction Page: 13 2.2. Size Page: 13 2.3. Molecular weight Page: 17 2.4. Diffusivity Page: 19 2.5. Sedimentation coefficient Page: 20 2.6. Osmotic pressure Page: 21 2.7. Electrostatic charge Page: 22 2.8. Solubility Page: 23 2.9. Partition coefficient Page: 24 2.10. Light absorption Page: 24 2.11. Fluorescence Page: 25 3. Mass transfer Page: 29 3.1. Introduction Page: 29 3.2. Molecular diffusion in liquid medium Page: 30 3.3. Measurement of diffusivity Page: 32 3.4. Estimation of diffusivity Page: 33 3.5. Diffusion of solutes in dense solid Page: 35 3.6. Diffusion of solutes in porous solid Page: 35 3.7. Convective mass transfer Page: 37 3.8. Experimental determination of mass transfer coefficient Page: 40 3.9. Estimation of mass transfer coefficient Page: 41 3.10. Inter-phase mass transfer Page: 42 3.11. Unsteady state mass transfer Page: 42 3.12. Equilibrium and rate processes Page: 45 4. Cell disruption Page: 49 4.1. Introduction Page: 49 4.2. Cells Page: 49 4.3. Cell disruption using bead mill Page: 53 4.4. Cell disruption using rotor-stator mill Page: 56 4.5. Cell disruption using French press Page: 56 4.6. Cell disruption using ultrasonic vibrations Page: 58 4.7. Cell disruption using detergents Page: 61 4.8. Cell disruption using enzymes Page: 61 4.9. Cell disruption using organic solvents Page: 62 4.10. Cell disruption by osmotic shock Page: 62 5. Precipitation Page: 65 5.1. Introduction Page: 65 5.2. Factors utilized for precipitation Page: 66 5.3. Precipitation using organic solvents Page: 69 5.4. Precipitation using anti-chaotropic salts Page: 72 5.5. Mechanism of precipitate formation Page: 74 6. Centrifugation Page: 79 6.1. Introduction Page: 79 6.2. Laboratory centrifuge Page: 80 6.3. Preparative centrifuge Page: 83 6.4. Ultracentrifugation Page: 87 7. Extraction Page: 89 7.1. Introduction Page: 89 7.2. Solvent systems Page: 89 7.3. Theory of extraction Page: 90 7.4. Aqueous two-phase extraction Page: 93 7.5. Batch extraction Page: 95 7.6. Single-stage continuous extraction using immiscible solvents Page: 99 7.7. Batch extraction using partially miscible solvents Page: 102 7.8. Cross-current continuous extraction using immiscible solvents Page: 103 7.9. Staged counter-current extraction Page: 104 7.10. Differential extraction Page: 109 7.11. Supercritical fluid extraction Page: 113 8. Adsorption Page: 121 8.1. Introduction Page: 121 8.2. Adsorbents Page: 122 8.3. Separation mechanisms Page: 123 8.4. Adsorption isotherms Page: 132 8.5. Diffusional limitations in adsorption processes Page: 135 8.6. Batch adsorption Page: 136 8.7. Packed bed adsorption Page: 140 8.8. Other types of adsorption devices Page: 147 9. Chromatography Page: 151 9.1. Introduction Page: 151 9.2. Chromatography system Page: 152 9.3. Theory of chromatography Page: 158 9.4. Shape and yield of a chromatographic peak Page: 167 9.5. Binary chromatography Page: 173 9.6. Hydrodynamic chromatography Page: 176 10. Filtration Page: 181 10.1. Introduction Page: 181 10.2. Theory of filtration Page: 181 10.3. Filter medium Page: 183 10.4. Driving force Page: 183 10.5. Constant pressure cake filtration Page: 184 10.6. Constant rate cake filtration Page: 190 10.7. Improvement of filtration efficiency Page: 191 10.8. Mode of operation Page: 192 10.9. Filtration equipment Page: 194 11. Membrane based bioseparation Page: 199 11.1. Introduction Page: 199 11.2. Classification of membrane processes Page: 205 11.3. Membrane equipment Page: 206 11.4. Ultrafiltration Page: 211 11.5. Microfiltration Page: 232 11.6. Dialysis Page: 235 11.7. Liquid membrane processes Page: 240 11.8. Membrane chromatography Page: 242 12. Miscellaneous bioseparation processes Page: 251 12.1. Introduction Page: 251 12.2. Electrophoresis Page: 251 12.3. Affinity ultrafiltration Page: 255 12.4. Field-flow fractionation Page: 255 Index Page: 257

Description:

Bioseparations engineering deals with the scientific and engineering principles involved in large-scale separation and purification of biological products. It is a key component of most chemical engineering/biotechnology/bioprocess engineering programmes. This book discusses the underlying principles of bioseparations engineering written from the perspective of an undergraduate course. It covers membrane based bioseparations in much more detail than some of the other books on bioseparations engineering. Based largely on the lecture notes the author developed to teach the course, this book is especially suitable for use as an undergraduate level textbook, as most other textbooks are targeted at graduate students.

Principles of Bioseparations Engineering PDF

6.98 MB·English

by Raja Ghosh

#Technology & Engineering #Chemical & Biochemical

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<p>Bioseparations engineering deals with the scientific and engineering principles involved in large-scale separation and purification of biological products. It is a key component of most chemical engineering/biotechnology/bioprocess engineering programmes. This book discusses the underlying principles of bioseparations engineering written from the perspective of an undergraduate course. It covers membrane based bioseparations in much more detail than some of the other books on bioseparations engineering. Based largely on the lecture notes the author developed to teach the course, this book is especially suitable for use as an undergraduate level textbook, as most other textbooks are targeted at graduate students.</p>

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Author:	Raja Ghosh
ISBN:	9789813106765
Language:	English
File Size:	6.98
Format:	PDF
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