Table Of ContentPRACTICAL
THIN-LAYER
CHROMATOGRAPHY
A Multidisciplinary Approach
PRACTICAL
THIN-LAYER
CHROMATOGRAPHY
A Multidisciplinary Approach
Edited by
Bernard Fried
Joseph Sherma
CRC Press
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Library of Congress Cataloging-in-Pubiication Data
Practical thin-layer chromatography : a multidisciplinary approach ; edited by Bernard Fried, Joseph Sherma
p. cm.
Includes bibliographical references and index.
ISBN 0-8493-2660-5
1. Thin-layer chromatography. I. Fried, Bernard, 1933-. II. Sherma, Joseph. H.
QD79.C8P73 1996
543'.08956-dc20
DNLM/DLC 95-52348
CIP
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PREFACE
After an introductory chapter on principles, techniques, and instrumentation, this book contains chapters
that describe the use of thin-layer chromatography (TLC) in eleven important scientific disciplines. This
approach is novel for books on TLC; most of them have a number of introductory chapters dealing with
theory, practice, and instrumentation, followed by applications chapters organized according to com
pound (analyte) type; contain a series of chapters on practice or applications; or are a random collection
of papers from a symposium. The chapter authors comprise an international roster of expert practitioners
of TLC in the most important application areas.
We proposed this new disciplinary approach not only to avoid redundancy with existing books in the
field but because we believe it provides thorough coverage of the principles, practices, and applications
of TLC for important sample and compound types directed specifically at workers in the most active
scientific fields.
We attempted to make this book as practical as possible. Authors were encouraged to include the
following topics in each chapter: an introduction that discusses the importance of TLC in the field and
compares TLC to other analytical techniques; sample collection and preparation; chromatographic
systems (layers and mobile phases); development techniques; detection, identification, and quantification
of zones; validation of results; specific protocols for important analyses; and references through 1994.
Chapters 2 to 6 cover fields of greatest interest to biologists and biochemists and Chapters 7 to 12
to chemists and analysts. Although the chapters were written by specialists in particular disciplines, the
information presented is also applicable to analyses in related fields. The book should be useful to all
scientists interested in the principles and practices of TLC. It will be of greatest interest to students,
teachers, researchers, clinicians, and technicians who wish to apply TLC in the disciplines covered in
the chapters and in related disciplines.
We encourage readers to suggest ways to improve the book, including additional topics and authors,
for the second edition.
Bernard Fried
Joseph Sherma
Lafayette College,
Easton, PA
June, 1995
LIST OF CONTRIBUTORS
Jan Bladek, Ph.D. M. A. Haseeb, Ph.D.
Institute of Chemistry Department of Microbiology, Immunology and
Military University of Technology Medicine
Warsaw, Poland State University of New York
Health Services Center
Giuseppe Ceramelli, Ph.D. Brooklyn, New York
Dipartimento Farmaco Chimico Technologico
Università Degli Studi di Siena, Italy Raka Jain, Ph.D.
Drug Dependence Treatment Centre
All India Institute of Medical Sciences
Pietro Corti, Ph.D.
New Delhi, India
Dipartimento Farmaco Chimico Technologico
Università Degli Studi di Siena, Italy
Mark Maloney, Ph.D.
Biology Department
Tibot Cserhàti, Ph.D.
Spelman College
Central Research Institute of Chemistry
Atlanta, Georgia
Hungarian Academy of Sciences
Budapest, Hungary
Illka Ojanpera, Ph.D.
Forensic Toxicology Division
Elena Dreassi, Ph.D.
Department of Forensic Medicine
Dipartimento Farmaco Chimico Technologico
University of Helsinki
Università Degli Studi di Siena, Italy
Helsinki, Finland
Richard D. Fell, Ph.D. Ali R. Shalaby, Ph.D.
Department of Entomology Food Technology and Dairy Sciences Department
Virginia Polytechnic Institute and State University National Research Centre
Blacksburg, Virginia Cairo, Egypt
Esther Forgacs, Ph.D. H. M. Stahr, Ph J).
Central Research Institute of Chemistry College of Veterinary Medicine
Hungarian Academy of Sciences University of Iowa
Budapest, Hungary Ames, Iowa
Bernard Fried, Ph.D. Paul J. Weldon, Ph.D.
Department of Biology Conservation and Research Center
Lafayette University Smithsonian Institute
Easton, Pennsylvania Front Royal, Virginia
CONTENTS
Chapter 1
Introduction to Techniques and Instrumentation..................................................................................................1
Tibor Cserhati and Ester Forgacs
Chapter 2
Thin-Layer Chromatography in Bacteriology...................................................................................................19
Mark Maloney
Chapter 3
Thin-Layer Chromatography in Plant Sciences................................................................................................33
Jacques Pothier
Chapter 4
Thin-Layer Chromatography in Parasitology...................................................................................................51
Bernard Fried
Chapter 5
Thin-Layer Chromatography in Studies in Entomology.................................................................................71
Richard D. Fell
Chapter 6
Thin-Layer Chromatography of Skin Secretions in Vertebrates..................................................................105
Paul Weldon
Chapter 7
Thin-Layer Chromatography in Clinical Chemistry......................................................................................131
Raka Jain
Chapter 8
Thin-Layer Chromatography in Environmental Analysis.............................................................................153
Jan Bladek
Chapter 9
Thin-Layer Chromatography in Food Analysis..............................................................................................169
Ali R. Shalaby
Chapter 10
Thin-Layer Chromatography in Forensic Toxicology....................................................................................193
Ilkka Ojanpera
Chapter 11
Thin-Layer Chromatography in Pharmaceutical Analysis............................................................................231
Flena Dreassi, Giuseppe Ceramelli, and Pietro Corti
Chapter 12
Thin-Layer Chromatography in Veterinary Toxicology.................................... 249
H. M. Stahr
Index...........................................................................................................................................................................265
