Download Practical Biotransformations: A Beginner's Guide PDF Free - Full Version
Download Practical Biotransformations: A Beginner's Guide by Gideon Grogan in PDF format completely FREE. No registration required, no payment needed. Get instant access to this valuable resource on PDFdrive.to!
About Practical Biotransformations: A Beginner's Guide
Content: Chapter 1: Biotransformations, Microbes and Enzymes. 1.1 Introduction. 1.2 Biotransformations. 1.3 Microorganisms. 1.4 Organism nomenclature. 1.5 Enzymes. 1.6 Types of Enzymatic reactions. 1.7 Enzymatic Cofactors. 1.8 Some Basic Characteristics of Enzyme Catalysis. 1.9 Types of Biocatalyst - Biotransformations by 'whole cells' or isolated enzymes. 1.10. Conclusion. Chapter 2: An overview of biocatalyst sources and web-based information. 2.1 Introduction. 2.2 Microbial culture collections. 2.3 Obtaining organisms from other research groups. 2.4 Selective Enrichments. 2.5 Metagenomics. 2.6 Enzyme Suppliers and Biocatalyst Development Companies. 2.7 Genome mining for biocatalysts. 2.8 Obtaining amino acid and gene sequence information on biocatalysts. 2.9 Obtaining DNA templates for cloning. 2.10 Custom Gene Synthesis. 2.11 Other interesting web resources for biocatalysis. 2.12 Conclusion. Chapter 3: Setting up a laboratory for biotransformations. 3.1 Introduction. 3.2 Microbiological Containment. 3.3 On containment issues and genetically-modified organisms. 3.4 Equipment for handling microorganisms. 3.5 Techniques and terms in microbiology - Sterility, Asepsis and Aseptic Technique. 3.6 Disposal of viable microbial waste and disinfection of reusable equipment. 3.7 Equipment for enzymology and molecular biology. 3.8 General reagents and chemicals in a Biotransformations Laboratory. 3.9 Conclusion. Chapter 4: A beginner's guide to preparative whole-cell microbial biotransformations. 4.1 Introduction. 4.2 Storage, maintenance and growth of microorganisms. 4.3 General Microbiological Methods. 4.4 Examples of Whole-cell Biotransformations using Bacteria. 4.5 Biotransformation by filamentous fungi and yeasts. 4.6 Whole-cell Biotransformations by recombinant strains of E. coli. 4.7 Conclusion. Chapter 5: A beginner's guide to biotransformations by commercially available isolated enzymes. 5.1 Introduction. 5.2 Lipases. 5.3 Hydrolytic Reactions using lipases. 5.4 Using lipases for acylation reactions. 5.5 Other hydrolases. 5.6 Commercially available Coenzyme-dependent Enzymes. 5.7 Carbon-carbon bond forming reactions. 5.8 Conclusion. Chapter 6: A beginner's guide to the isolation and analysis and use of home-grown enzyme biocatalysts. 6.1 Introduction. 6.2 Cell growth and harvesting. 6.3 Cell disruption. 6.4 A typical procedure for making a cell extract from a recombinant strain of E. coli. 6.5 Purification of enzymes - a brief guide. 6.6 Techniques for Protein Purification. 6.7 Isolation of recombinant enzymes using histidine tags. 6.8 Estimation of protein concentration. 6.9 Concentrating protein samples by centrifugation. 6.10 Analysis of protein samples by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE). 6.11 Examples of enzyme assays. 6.12 Using home-grown enzymes for biotransformations ? Some recent examples. 6.13 Conclusion. Chapter 7: An introduction to basic gene cloning for the production of designer biocatalysts. 7.1 Introduction. 7.2 Background to gene cloning. 7.3 Gene amplification by polymerase chain reaction (PCR). 7.4 DNA fragment analysis by agarose electrophoresis. 7.5 Gene cloning. 7.6 Analysis by DNA sequencing. 7.7 Troubleshooting the gene amplification and cloning process. 7.8 Ligation-Independent Cloning. 7.9 Gene Expression in E. coli. 7.10 Conclusion. Chapter 8: Engineering Enzymes. 8.1 Introduction. 8.2 Site-directed or targted mutagenesis as a tool for investigating enzyme mechanism or altering catalytic attributes. 8.3 A site-directed mutagenesis experiment. Considerations and practise. 8.4 Engineering using random mutagenesis. Directed Evolution of Enzymes. 8.5 Combining rational and random mutagenesis for biocatalyst improvement. 8.6 Exploiting catalytic promiscuity for creating new enzyme activities. 8.7 Designing enzymes in silico. 8.8 Conclusion. Appendices. 1. Structures of the proteinogenic amino acids. 2. Structures of bases found in nucleic acids. 3. The Genetic Code. 4. Recipes for Microbiological Growth Media. 5. Biological buffers. 6. Ammonium sulphate fractionation table. 7. Restriction enzymes and restriction sites
Detailed Information
| Author: | Gideon Grogan |
|---|---|
| Publication Year: | 2009 |
| ISBN: | 9781405193672 |
| Pages: | 353 |
| Language: | English |
| File Size: | 4.207 |
| Format: | |
| Price: | FREE |
Safe & Secure Download - No registration required
Why Choose PDFdrive for Your Free Practical Biotransformations: A Beginner's Guide Download?
- 100% Free: No hidden fees or subscriptions required for one book every day.
- No Registration: Immediate access is available without creating accounts for one book every day.
- Safe and Secure: Clean downloads without malware or viruses
- Multiple Formats: PDF, MOBI, Mpub,... optimized for all devices
- Educational Resource: Supporting knowledge sharing and learning
Frequently Asked Questions
Is it really free to download Practical Biotransformations: A Beginner's Guide PDF?
Yes, on https://PDFdrive.to you can download Practical Biotransformations: A Beginner's Guide by Gideon Grogan completely free. We don't require any payment, subscription, or registration to access this PDF file. For 3 books every day.
How can I read Practical Biotransformations: A Beginner's Guide on my mobile device?
After downloading Practical Biotransformations: A Beginner's Guide PDF, you can open it with any PDF reader app on your phone or tablet. We recommend using Adobe Acrobat Reader, Apple Books, or Google Play Books for the best reading experience.
Is this the full version of Practical Biotransformations: A Beginner's Guide?
Yes, this is the complete PDF version of Practical Biotransformations: A Beginner's Guide by Gideon Grogan. You will be able to read the entire content as in the printed version without missing any pages.
Is it legal to download Practical Biotransformations: A Beginner's Guide PDF for free?
https://PDFdrive.to provides links to free educational resources available online. We do not store any files on our servers. Please be aware of copyright laws in your country before downloading.
The materials shared are intended for research, educational, and personal use in accordance with fair use principles.
