Polyurethane-based Scaolds for Bone Tissue Engineering Gifty AJ Tetteh

351 Pages·2017·24.46 MB·English

Polyurethane-based Sca�olds
for
Bone Tissue Engineering
The Role of Hydroxyapatite Particles, Solvent Combinations,
Electrospun Fibre Orientations, In Vivo & In Vitro Characterisation,
and Particulate Leached Foams for creating 3-D Bone Models.
Gifty A. J. Tetteh
A thesis submitted in partial fulfilment of the requirements
for the degree of Doctor of Philosophy
Department of Material Science & Engineering
University of She�eld
United Kingdom
September, 2016.
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Awards and Conferences
Awards & Prizes
Learned Society Travel Grant, University of She�eld Aug, 2015
•
European Society of Biomechanics Travel Award July, 2015
•
Learned Society Travel Grant, University of She�eld Mar, 2015
•
Best Biomechanics Paper, MECbioeng14, Imperial College Sept, 2014
•
Kroto Family Science Education Prize (Joint Winner) July, 2014
•
Best Poster Award, 10th Bose User Group Meeting June, 2014
•
BestBiomaterials&TissueEngineeringPosterAward May, 2014
•
Santander Research Mobility Award Nov, 2013
•
Learned Society Travel Grant, University of She�eld Oct, 2013
•
Ghana Education Trust Fund Scholarship for PhD Mar, 2012
•
Publications
Journal Papers
1. G.Tetteh, A.S.Khan, R.M.Delaine-Smith, G.C.Reilly & I.U.Rehman,“Electrospun
polyurethane / hydroxyapatite bioactive sca�olds for bone tissue engineering: The
role of solvent and hydroxyapatite particles”. Journal of the Mechanical Behavior
of Biomedical Materials. (2014) Vol. 39, pp. 95–110
Conference Proceedings
2. G. Tetteh, R.Delaine-Smith, N.H. Green, G.C.Reilly, July (2016), “Collagen and
Mineral Deposition by MLO-A5 Late-Stage Osteoblasts, is Guided by the Fibre
Alignment of Electrospun Sca�olds”, Proc. of the 22nd Congress of the European
Society of Biomechanics, Lyon, France.
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ii AWARDS AND CONFERENCES
3. G. Tetteh,M.Pogorielov,I.U.Rehman,G.C.Reilly,May(2016),“A3-dimensional
biomimetic in vitro bone model for testing small orthopaedic implants”, Proc. of
the 10th World Biomaterials Congress, Montreal, Canada.
4. G. Tetteh, I.U. Rehman, G.C. Reilly, August (2015), “in vivo and in vitro
Characterization of Porous Polyurethane-Hydroxyapatite Sca�olds as A Bone
Substitute,” Proc. of the European Society for Biomaterials Conference, Krakow,
Poland.
5. G. Tetteh, S. Puwanan, I.U. Rehman, G.C. Reilly, July (2015), “A Tissue
Engineered Bone Membrane for Cleft Palate Repair”, Proc. of the 21st Congress
of the European Society of Biomechanics, Prague, Czech Republic.
6. G. Tetteh, M. Pogorielov, I.U. Rehman, G.C. Reilly, June (2015), “Developing
Mechanically-Optimized&HighlyPorousPolyurethane-HydroxyapatiteConstructs
for Bone Tissue Engineering”, Proc. of the 14th Annual Conference of the UK
Society for Biomaterials, Belfast, Northern Ireland.
7. G. Tetteh, I.U. Rehman, G.C. Reilly, March (2015), “Using Tissue Engineering
to develop an in vitro 3D Bone Model,” Proc. of the Orthopedic Research Society
Conference, Las Vegas, USA.
8. G. Tetteh, I.U. Rehman, G.C. Reilly, Sept (2014), “Engineering an in vitro 3D
BoneModelforImplantTesting,”Proc. oftheMedicalEngineeringCentre’sAnnual
Meeting and Bioengineering 14, London, UK.
9. G. Tetteh, I.U. Rehman, G.C. Reilly, August (2014), “Using Bioactive Sca�olds to
synthesize an in vitro 3D Bone Model for Implant Testing,” Proc. of the European
Society for Biomaterials Conference, Liverpool, UK.
10. G. Tetteh, I.U. Rehman, G.C. Reilly, July (2014), “Particulate Leached Sca�olds
foranin vitro3DBoneModelforImplantTesting,”Proc. ofthe7thWorldCongress
of Biomechanics, Boston, USA.
11. G. Tetteh, I.U. Rehman, G.C. Reilly, July (2014), “Bioactive Sca�olds as Bone
Templates for in vitro Testing,” European Cells and Materials Vol. 28. Suppl. 4,
pp. 47.
12. G. Tetteh, I.U. Rehman, G.C. Reilly, May (2014), “Using composite sca�olds to
engineer bone templates for in vitro testing,” Proc. of the 2014 Bone Research
Society Conference, She�eld, UK.
13. G. Tetteh, I.U. Rehman, G.C. Reilly, December (2013) “A
polyurethane-hydroxyapatite composite sca�old as a template for an in vitro
bone model,” Proc. of the ICMOBT 2013, Stiges, Spain.
14. G. Tetteh, I.U. Rehman, G.C. Reilly, “Polyurethane-Hydroxyapatite composite
sca�olds for in vitro bone tissue engineering,” European Cells and Materials Vol.
26. Suppl. 7, 2013 (page 38)
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Conference Presentations
Oral Presentations
22nd Congress of the European Society of Biomechanics, Lyon, France.
•
European Society for Biomaterials Conference, Krakow, Poland.
•
21st Congress of the European Society of Biomechanics, Prague, Czech Republic.
•
14th Annual Conference of the UK Society for Biomaterials, Belfast, Northern
•
Ireland.
Medical Engineering Centre’s Annual Meeting and Bioengineering 14, London, UK.
•
2013 Tissue and Cell Engineering Society Conference, Cardi�, UK
•
Poster Presentations
10th World Biomaterials Congress, Montreal, Canada.
•
Orthopedic Research Society Conference, Las Vegas, USA.
•
European Society for Biomaterials Conference, Liverpool, UK.
•
7th World Congress of Biomechanics, Boston, USA.
•
2014 Tissue and Cell Engineering Society Conference, Newcastle, UK
•
10th Bose User Group Meeting
•
2nd Year Postgraduate Research Day
•
2014 Bone Research Society Conference, She�eld, UK.
•
ICMOBT 2013, Stiges, Spain.
•
7th MSC Meeting, 2013, Birmingham, UK.
•
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Dedication
to Akwesi & Kwame....
I could not have done this without you
two!
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Acknowledgements
I am grateful to God for seeing me through the four years of my PhD. There were
di�cult times and tough periods but I am thankful He kept me till the end.
I would like to thank my supervisors, Dr. Gwendolen Reilly and Dr. Ihtesham
Rehman for their support, guidance and encouragement throughout my PhD. I would
also like to thank Dr. Maksym Pogorielov, Dr. Ceyla Yorucu, Dr. Chris Hill and
everyone in the Biomaterials & Tissue Engineering Laboratory at the University of
She�eld, who contributed in one way or the other towards my research.
I am thankful to the Government of Ghana and the Ghana Education Trust
Fund (GETFund) for providing financial support for this research.
My deepest appreciation goes to my husband, my mother and my brother, for their
understanding and patience during the long hours I spent in the lab on most days and
the entire period I spent writing this thesis. A very special thank you to all my friends
and family for their endless love and support.
My final thank you goes to Thomas and Gwen again, without whom none of this
would have been possible. I am very grateful for their unfailing support all through my
PhD, especially during the times I was ill.
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