Platelets: Receptors, Adhesion, Secretion Part B

560 Pages·1992·16.29 MB·English

by  Jacek J. Hawiger (Eds.)

Contributors to Volume 215
Article numbers are in parentheses following the names of contributors.
Affiliations listed are current.
BURT ADELMAN (36), Department of Medi- AICIRTAP CARLSON (36), Department of
cal Research, Biogen Inc., Cambridge Laboratory Medicine, Hunter Holmes
Massachusetts 24120 McGuire Veterans Administration Cen-
RALPH M. ALBRECHT (39), Department of ter, Richmond, Virginia 94232
Animal Health and Biomedical Sciences, JEANNINE M. CLEMETSON (24), Theodor
University of Wisconsin, Madison, Wis- Kocher Institute, University of Berne,
consin 60735 2103-HC Berne, Switzerland
KALWANT S, AUTHI (2), Platelet Section, HTENNEK J. NOSTEMELC (26), Theodor Ko-
Thrombosis Research Institute, Chelsea, chef Institute, University of Berne, -HC
London SW3 6LR, England 2103 Berne, Switzerland
ALLESSOR BADER (23), Hemophilia and YRRAB S. COLLER (24), Department of Med-
Thrombosis Center, Policlinico Hospital, icine, Division of Hematology, State Uni-
University of Milan, Milan 20122, Italy versity of New York at Stony Brook,
DIANA S. YELSDRAEB (37), Division of Pedi- Stony Brook, New York 49711
atric Hematology, Yale University School NANCY C. COLLIER (10), Department of
of Medicine, New Haven, Connecticut Molecular Microbiology, Washington
01560 University School of Medicine, St. Louis,
MICHAEL C. BERNDT (16), Baker Medical Missouri 01136
Research Institute, Prahrau VIC 3181, ROBERT W. COLMAN (14), Sol Sherry
Australia. Thrombosis Research Center and Depart-
LORAC ANN BONNER (17), Department of ment of Medicine, Temple University
Pharmacology, Vanderbilt University, School of Medicine, Philadelphia, Penn-
Nashville, Tennessee 23273 sylvania 04191
ATREBOR F. COLMAN (14), Department of
DANILO S. CIVOKSOB (28), Department of
Chemistry and Biochemistry, University
Biochemistry, Queen's University, Kings-
of Delaware, Newark, Delaware 61791
ton, Ontario KTL 3N6, Canada
NEVILLE CRAWFORD (2), Department of
JANET K. SELYOB (6), Vanderbilt University
Biochemistry, Hunterian Institute, Royal
School of Medicine, Nashville, Tennessee
College of Surgeons, London WC2A
23273
3PN, England
SUZANNE BRANDON (17), Department of LEON W. CUNNINGHAM (34), Department
Pharmacology, Vanderbilt University,
of Biochemistry, Vanderbilt University
Nashville, Tennessee 23273
School of Medicine, Nashville, Tennessee
M. JOHAN NAMKEORB (3), Divisions of He- 23273
matology/Oncology, Departments of SEMAJ L. DANIEL (8), Department of Phar-
Medicine, Department of Veterans Af- macology and Thrombosis Research Cen-
fairs Medical Center, and Cornell Univer- ter, Temple University, Philadelphia,
sity Medical College, New York, New Pennsylvania 04191
York 01001
ASEREHT A. DAVIES (15), Department of
JOSEPH BRYAN (9), Department of Cell Biochemistry, Boston University School
Biology, Baylor College of Medicine, of Medicine, Boston, Massachusetts
Houston, Texas 03077 81120
ix
X CONTRIBUTORS TO VOLUME 215
LUIGI DEMARCO (23), Servisio Immunotras- Committee on Vascular Biology, The
fusionale e Analisi Cliniche, Centro di Ri- Scripps Research Institute, La Jolla, Cal-
ferimento Oncologico, Aviano, Por- ifornia 73029
denone, Italy NEEDURHSAN HACK (2), Department of
NEVETS E. DOMINO (17), Department of Medicine, University of Toronto, -oT
Obstetrics and Gynecology, University ronto, Ontario M5S lAB, Canada
of Michigan, Ann Arbor, Michigan YNOHTNA S. HAJEK (33), Department of
90184 Opthamology, Bascom Palmer Eye Insti-
WILLIAM R. FIGURES (14), Thrombosis Re- tute, University of Miami School of Medi-
search Center and Hematology Division, cine, Miami, Florida 10133
Temple University School of Medicine, TREBOR I. HANDIN (25, 36), Division of He-
Philadelphia, Pennsylvania 04191 matology, Department of Medicine,
ECNERUAL FITZGERALD (22), University of Brigham and Women's Hospital, Harvard
Utah, Salt Lake City, Utah 21148 Medical School, Boston, Massachusetts
JANE HTYSROF (27), Committee on Vascular 51120
Biology, The Scripps Research Institute, JOAN T. NOMRAH (4), National Institutes of
La Jolla, California 73029 Diabetes and Digestive and Kidney Dis-
JOAN E. B. Fox (6), Gladstone Institute of eaes, Division of Diabetes, Endocrinol-
Cardiovascular Disease, University of ogy, and Metabolic Diseases, National
California, San Fransisco, San Fran- Institutes of Health, Bethesda, Maryland
sisco, California 14149 29802
MIRIAM H. FUKAMI (5), Department of -DiB KECAJ HAWIGER (1, 12, 21), Department of
chemistry, University of Bergen, N-5009 Microbiology and Immunology, A-5321
Bergen, Norway MCN, Vanderbilt University School of
Medicine, Nashville, Tennessee 23273
DAVID P. GEANEY (18), Department of -nilC
ical Pharmacology, Radcliffe Infirmary, LESLIE HOWARD (38), Hematology/Oncol-
Oxford, OX2 6HE, England ogy Section, Veterans Administration,
Medical Center, East Orange, New Jer-
MARK H. GINSBERG (27), Committee on
sey 91070
Vascular Biology, The Scripps Research
Institute, La Jolla, California 73029 AIHTNYC J. HUMPHREYS (19), Department
of Microbiology, College of Physicians
D. G. GRAHAME-SMITH (18), University De-
and Surgeons, Columbia University, New
partment of Clinical Pharmacology, Rad-
York, New York 23001
cliffe Infirmary, Oxford 2XO 6HE, En-
gland G. A. JAMIESON (4), Cell Biology Depart-
ment, Holland Laboratory, American
SALOHCIN J. GRECO (4), Cell Biology De-
Red Cross, Rockville, Maryland 55802
partment, Holland Laboratory, American
Red Cross, Rockville, Maryland 55802 J. HEINRICH JOIST (33), Department ofln-
SHERYL M. GREENBERG (15), Division of ternal Medicine, Division of Bone Mar-
Hematology, Brigham and Women's row Transplantation, Hematology, and
Hospital, Boston, Massachusetts 51120 Oncology, St. Louis University Medical
Center, St. Louis, Missouri 01136
JUDITH S. DRAGNEERG (31), Department of
Molecular and Experimental Medicine, EFSTATHIA L. KALOMIRIS (26), Quality
and Committee on Vascular Biology, ehT Control Assurance, Hoffman-La Roche,
Scripps Research Institute, La Jolla, Cal- Nutley, New Jersey 01170
ifornia 73029 SIMON KARPATKIN (38), Department of
JOHN H. GRIFFIN (31), Department of Mo- Medicine, New York University Medical
lecular and Experimental Medicine, and Center, New York, New York 61001
CONTRIBUTORS TO VOLUME 215 xi
MATTHEW E. KENNEDY (17), Department JOHN R. PETERS (18), Department of Medi-
of Pharmacology, Vanderbilt University, cine, University Hospital of Wales, Car-
Nashville, Tennessee 23273 diff CF4 4XN, United Kingdom
HTEBASILE KOLLER (32), Institut fi~r Medi- DAVID R. PHILLIPS (16, 22, 35), COR Thera-
zinischen Physiologie, Universitgit Wien, peutics, Inc., South San Fransisco, Cali-
Vienna, Austria fornia 08049
FRANZ KOLLER (32), Institutfiir Allegemeine EDWARD F. PLOW (27), Committee on Vas-
Biochemie, Universitgit Wien, und cular Biology, The Scripps Research In-
Ludwig-Boltemann Forschungs-Stelle fiir stitute, La Jolla, California 73029
Biochemie, Vienna, Austria MARY G. REPASKE (17), Department of
SALOCIN J. KOTITE (34), Department of Pharmacology, Vanderbilt University,
Biochemistry, Vanderbilt University Nashville, Tennessee 23273
School of Medicine, Nashville, Tennessee CLIFFORD C. SDLONYER (6), Gladstone In-
23273 stitute of Cardiovascular Disease, Uni-
NANCY E. LARSEN (15), Biomatrix, Inc., versity of California, San Fransisco, San
Ridgefield, New Jersey 07657 Fransisco, California 14149
LEE E. LIMBIRD (17), Department of Phar- NORAHS ROSENBERG SCHAIER (7), JMR
macology, Vanderbilt University, Nash- Consulting, Fair Lawn, New Jersey 01470
ville, Tennessee 23273 GARY RUDNIC~ (19), Department of Phar-
HPESOJ C. LOFTUS (39), Committee on Vas- macology, Yale University School of
cular Biology, The Scripps Research In- Medicine, New Haven, Connecticut
stitute, La Jolla, California 73029 01560
JOSEPH LOSCALZO (25), Department of OIREVAZ M. RUCOERI (23), Roon Research
Medicine, Brigham and Women's Hospi- Laboratory for Arteriosclerosis and
tal, Harvard Medical School, Boston, Thrombosis, Department of Molecular
Massachusetts 51120 and Experimental Medicine, and Commit-
tee on Vascular Biology, ehT Scripps Re-
DLANOD E. ENALRAFCAM (13), Department
search Institute, La Jolla, California,
of Medicine, The University of Iowa,
73029
Iowa City, Iowa 24225
NASUS RUSSELL (23), Roon Research Labo-
KENNETH G. MANN (28, 29), Department of
ratory for Arteriosclerosis and Thrombo-
Biochemistry, University of Vermont Col-
sis, Department of Molecular and Experi-
lege of Medicine, Burlington, Vermont
mental Medicine, The Scripps Research
50450
Institute, La Jolla, California 73029
DEANE F. MOSHER (39), Department of
L. MARIE ECRAECS (14), Department of Hu-
Medicine, University of Wisconsin, Madi-
man Genetics, Howard Hughes Medical
son, Wisconsin 60735
Institute, University of Pennsylvania
MICHAEL E. NESHEIM (28, 29), Depart- School of Medicine, Philadelphia, Penn-
ments of Medicine and Biochemistry, sylvania 40191
Queen's University, Kingston, Ontario
WOLF6ANG SCHNIPPERINC (24), Central
K7L 3N6, Canada
Laboratory, Swiss Red Cross Blood
OLUFUNKE E. ERADNUROLO (39), Depart- Transfusion Service, CH-3000 Berne ,22
ment of Pharmacology and Therapeutics, Switzerland
University of Illorin, Illorin, Nigeria
YELSEL E. SCVDDER (26), Department of
LESLIE PARISE (22), Department of Pharma- Medicine, Division of Hematology, State
cology, University of North Carolina, University of New York at Stony Brook,
Chapel Hill, North Carolina 99572 Stony Brook, New York 49711
xii CONTRIBUTORS TO VOLUME 215
JAMES R. SELLERS (8), Laboratory of Mo- lege of Medicine, Burlington, Vermont
lecular Cardiology, National Heart, Lung 50450
and Blood Institute, National Institutes of RUSSELL P. TRACY (28), Department of
Health, Bethesda, Maryland 29802 Pathology, University of Vermont, Bur-
ARBAS NAMLUHS (38), Department of Medi- lington, Vermont 50450
cine, New York University Medical Cen- FRANK H. VALONE (20), Department of
ter, New York, New York 61001 Medicine, Dartmouth-Hitchcock Medical
TTOCS R. SNOMMIS (39), Department of An- Center, Lebanon, New Hampshire 65730
imal Health and Biomedical Sciences, PETER N. WALSH (30), Thrombosis Re-
University of Wisconsin, Madison, Wis- search Center, Temple University School
consin 60735 of Medicine, Philadelphia, Pennsylvania
ELIZABETH R. SIMONS (15), Department of 04191
Biochemistry, Boston University School KUAN WANG (10), Department of Chemis-
of Medicine, Boston, Massachusetts try and Biochemistry, Welch Hall 4.230,
81120 University of Texas at Austin, Austin,
DIPALI SINHA (30), Department of Bio- Texas 7611-21787
chemistry, Temple University School of SEMAJ G. WHITE (11), Departments of Lab-
Medicine, Philadelphia, Pennsylvania oratory Medicine, Pathology, and Pedi-
04191 atrics, University of Minnesota Medical
SEMAJ V. SORATS (34), Department of Mo- School, Minneapolis, Minnesota 55455
lecular Biology, Vanderbilt University, SAERDNA N. WICKI (24), ANAWA Labora-
Nashville, Tennessee 53273 tories, CH-8602 Wangen, Switzerland
BEAT STEINER (22, 24), Pharma Division AM't" L. WILSON (17), Department of Phar-
Preclinical Research, Hoffman-La macology, Vanderbilt University, Nash-
Roche, CH-4002 Basle, Switzerland ville, Tennesse 23273
SHEILA TIMMONS (21), Department of -iM ERODOEHT S. NAMREMMIZ t (23), Roon Re-
crobiology and Immunology, Vanderbilt search Laboratory for Arteriosclerosis
University School of Medicine, Nashville, and Thrombosis, Department of Molecu-
Tennessee 23273 lar and Experimental Medicine, ehT
PAULA B. TRACY (28, 29), Department of Scripps Research Institute, La Jolla, Cal-
Biochemistry, University of Vermont -loC ifornia 73029
Deceased.
Preface
Blood platelets are tiny corpuscles that continuously survey the inner
lining of blood vessels, the vascular endothelium. Any break in the conti-
nuity of the vessel wall, leading to hemorrhage, or a break in an athero-
sclerotic plaque is met with an instant response from platelets. When
platelets contact the zone of injury, they spread, aggregate, and form
thrombi that seal off the break. The membrane is transformed into batter-
ies of receptors for adenine nucleotides, catecholamines, adhesive mole-
cules, and clotting factors. The platelet interior receives a flux of calcium,
flexes its contractile apparatus, and becomes a furnace for enzymatic
oxidation of arachidonic acid into endoperoxides which are then trans-
formed into thromboxane ,2A a potent vasoconstrictor and platelet
agonist. The platelet storage granules secrete their constituent molecules,
adenosine phosphate, serotonin, adhesive proteins, and coagulation fac-
tor V, that are targeted toward their own receptors and receptors on other
platelets. Clotting enzymes, assembled on the platelet membrane as the
prothrombinase complex, generate thrombin from prothrombin. After se-
cretion of serotonin its reuptake involves two membrane mechanisms
akin to those in neurons. The platelet, hardly a cell, has gained recogni-
tion in human cell biology as a cellular element endowed with a membrane
that bears the highest density of receptors per surface unit area among the
known blood cells. Mitogenic growth factors, stored in and secreted from
platelet ~ granules, stimulate migration and proliferation of vascular
smooth muscle cells and fibroblasts producing the extracellular matrix.
An increasing number of biochemists, cell biologists, hematologists,
neuropharmacologists, and pathologists are working with blood platelets
as a useful system to study the processes of adhesion, neurotransmitter
uptake, secretion, and their regulation. Therefore, Part A (Volume 169)
was devoted to the methods of platelet isolation and to the study of their
adhesive and secretory functions. This volume (Part B) includes the isola-
tion of subcellular organelles and their characterization, and a spectrum
of methods for analyzing platelet receptors.
The idea for these volumes, comprising modern methods of platelet
analysis, germinated when the late Editor-in-Chief, Sidney Colowick, en-
couraged me to embark on this task. Sidney Colowick's untimely death
left us not only without one of the founding fathers of this monumental
series, but also without a most inspiring friend and advisor. His legacy
cannot be measured in words. He was an example of exquisite intellectual
elegance in every aspect of his scientific activities. His gentle discourse
iiix
xiv ECAFERP
and unassuming demeanor were matched by his all encompassing mind
and heart. These two volumes on platelets are dedicated to him.
It has been a genuine pleasure for me to interact with the staff of
Academic Press. They have been helpful and patient in dealing with the
difficulties encountered during the preparation of these volumes. In my
unit at the New England Deaconess Hospital and Harvard Medical
School, Ms. Sheila Timmons and Ms. Marie Bingyou, and later at Van-
derbilt University, Ms. Carol Walter, were enormously resourceful in
helping me organize and edit these two volumes. Finally, I offer my
deepest appreciation to my colleagues, the contributing authors, for their
wisdom, expertise, and scholarship.
JACEK HAWIGER
METHODS IN ENZYMOLOGY
EMULOV I. Preparation and Assay of Enzymes
Edited by SIDNEY P. COLOWICK DNA NATHAN O. KAPLAN
EMULOV II. Preparation and Assay of Enzymes
Edited by SIDNEY P. COLOWICK DNA NATHAN O. KAPLAN
EMULOV III. Preparation and Assay of Substrates
Edited by SIDNEY P. COLOWICK DNA NATHAN O. KAPLAN
EMULOV IV. Special Techniques for the Enzymologist
Edited by SIDNEY P. COLOWICK DNA NATHAN O. KAPLAN
EMULOV V. Preparation and Assay of Enzymes
Edited yb SIDNEY P. COLOWICK DNA NATHAN O. KAPLAN
EMULOV VI. Preparation and Assay of Enzymes (Continued)
Preparation and Assay of Substrates
Special Techniques
Edited yb SIDNEY P. COLOWICK DNA NATHAN O. KAPLAN
EMULOV VII. Cumulative Subject Index
Edited yb SIDNEY P. COLOWICK DNA NATHAN O. KAPLAN
EMULOV VIII. Complex Carbohydrates
Edited yb ELIZABETH F. NEUFELD DNA VICTOR GRUBSNIG
VOLUME IX. etardyhobraC Metabolism
Edited yb WILLIS A. WOOD
EMULOV X. Oxidation and Phosphorylation
Edited by RONALD W. KOORBATSE DNA MAYNARD E. PULLMAN
EMULOV XI. Enzyme Structure
Edited yb C. H. W. HIRS
EMULOV XII. Nucleic Acids (Parts A and B)
Edited by LAWRENCE NAMSSORG DNA KIVIE MOLDAVE
EMULOV XIII. Citric Acid Cycle
Edited by J. M. NIETSNEWOL
VOLUME XIV. Lipids
Edited yb J. M. NIETSNEWOL
EMULOV XV. Steroids and Terpenoids
Edited yb RAYMOND B. CLAYTON
VOLUME XVI. Fast Reactions
Edited by KUSTIN KENNETH
xv
xvi SDOHTEM NI YGOLOMYZNE
EMULOV XVII. Metabolism of Amino Acids and Amines (Parts A and B)
Edited by TREBREH ROBAT DNA WHITE CELIA ROBAT
EMULOV XVIII. Vitamins and Coenzymes (Parts A, B, and C)
Edited yb DLANOD B. McCORMICK DNA LEUMEL D. THGIRW
EMULOV XlX. Proteolytic Enzymes
Edited by EDURTREG E. NNAMLREP DNA OLZSAL DNAROL
EMULOV XX. Nucleic Acids and Protein Synthesis (Part C)
Edited by KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXI. Nucleic Acids (Part D)
Edited yb ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXII. Enzyme Purification and Related Techniques
Edited yb WILLIAM B. YBOKAJ
EMULOV XXIII. Photosynthesis (Part A)
Edited yb NAS YNOHTNA ORTEIP
EMULOV XXIV. Photosynthesis and Nitrogen Fixation (Part B)
Edited yb NAS YNOHTNA ORTEIP
EMULOV XXV. Enzyme Structure (Part B)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVI. Enzyme Structure (Part C)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVII. Enzyme Structure (Part D)
Edited by C. H. W. HIRS DNA EGRES N. FEEHSAMIT
EMULOV XXVIII. Complex Carbohydrates (Part B)
Edited by ROTCIV GRUBSNIG
EMULOV XXIX. Nucleic Acids and Protein Synthesis (Part E)
Edited by ECNERWAL NAMSSORG DNA KIVlE EVADLOM
EMULOV XXX. Nucleic Acids and Protein Synthesis (Part F)
Edited yb KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXXI. Biomembranes (Part A)
Edited by SIDNEY REHCSIELF DNA RETSEL REKCAP
EMULOV XXXII. Biomembranes (Part B)
Edited by SIDNEY REHCSIELF DNA RETSEL REKCAP
EMULOV XXXIII. Cumulative Subject Index Volumes I-XXX
Edited by AHTRAM G. DENNIS DNA DRAWDE A. SINNED
EMULOV XXXIV. Affinity Techniques (Enzyme Purification: Part B)
Edited by WILLIAM B. YBOKAJ DNA MEIR KEHCLIW
EMULOV XXXV. Lipids (Part B)
Edited by JOHN i. NIETSNEWOL
SDOHTEM NI YGOLOMYZNE xvii
EMULOV XXXVI. Hormone Action (Part A: Steroid Hormones)
Edited yb BERT W. O'MALLEY DNA JOEL G. NAMDRAH
EMULOV XXXVII. Hormone Action (Part B: Peptide Hormones)
Edited yb BERT W. O'MALLEY DNA JOEL G. NAMDRAH
EMULOV XXXVIII. Hormone Action (Part C: Cyclic Nucleotides)
Edited yb JOEL G. NAMDRAH DNA BERT W. O'MALLEY
EMULOV XXXIX. Hormone Action (Part D: Isolated Cells, Tissues, and Organ
Systems)
Edited yb JOEL G. NAMDRAH DNA BERT W. O'MALLEY
EMULOV XL. Hormone Action (Part E: Nuclear Structure and Function)
Edited by BERT W. O'MALLEY DNA JOEL G. NAMDRAH
EMULOV XLI. Carbohydrate Metabolism (Part )B
Edited by W. A. DOOW
EMULOV XLII. Carbohydrate Metabolism (Part C)
Edited yb W. A. DOOW
EMULOV XLIII. Antibiotics
Edited yb JOHN H. HSAH
EMULOV XLIV. Immobilized Enzymes
Edited yb KLAUS HCABSOM
EMULOV XLV. Proteolytic Enzymes (Part B)
Edited yb OLZSAL DNAROL
EMULOV XLVI. Affinity Labeling
Edited yb WILLIAM B. YBOKAJ DNA MEIR KEHCLIW
EMULOV XLVII. Enzyme Structure (Part E)
Edited yb C. H. W. HIRS DNA EGRES N. FEEHSAMIT
EMULOV XLVIII. Enzyme Structure (Part F)
Edited yb C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XLIX. Enzyme Structure (Part G)
Edited yb C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV L. Complex Carbohydrates (Part C)
Edited by ROTCIV GRUBSNIG
EMULOV LI. Purine and Pyrimidine Nucleotide Metabolism
Edited by AICIRTAP A. HOFFEE DNA YRAM ELLEN SENOJ
EMULOV LII. Biomembranes (Part C: Biological Oxidations)
Edited yb SIDNEY REHCSIELF DNA RETSEL REKCAP
EMULOV LIII. Biomembranes (Part D: Biological Oxidations)
Edited by SIDNEY REHCSIELF DNA RETSEL REKCAP
EMULOV LIV. Biomembranes (Part E: Biological Oxidations)
Edited by SIDNEY REHCSIELF DNA RETSEL REKCAP
XVlll °,° METHODS IN ENZYMOLOGY
EMULOV LV. Biomembranes (Part F: Bioenergetics)
Edited yb SIDNEY REHCSIELF DNA RETSEL REKCAP
EMULOV LVI. Biomembranes (Part G: Bioenergetics)
Edited by SIDNEY REHCSIELF DNA RETSEL REKCAP
EMULOV LVII. Bioluminescence and Chemiluminescence
Edited yb ENELRAM A. DELUCA
EMULOV LVIII. Cell Culture
Edited by B. WILLIAM YBOKAJ DNA ARI NATSAP
EMULOV LIX. Nucleic Acids and Protein Synthesis (Part G)
Edited by KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV LX. Nucleic Acids and Protein Synthesis (Part H)
Edited yb KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV .16 Enzyme Structure (Part H)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV .26 Vitamins and Coenzymes (Part D)
Edited by DLANOD I. KCIMROCcM DNA LEUMEL D. THGIRW
EMULOV 63. Enzyme Kinetics and Mechanism (Part A: Initial Rate and Inhibi-
tor Methods)
Edited yb DANIEL L. HCIRUP
EMULOV 64. Enzyme Kinetics and Mechanism (Part B: Isotopic Probes and
Complex Enzyme Systems)
Edited yb DANIEL L. HCIRUP
EMULOV 65. Nucleic Acids (Part I)
Edited yb ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV .66 Vitamins and Coenzymes (Part E)
Edited yb DLANOD B. McCoRMICK DNA LEUMEL D. THGIRW
EMULOV .76 Vitamins and Coenzymes (Part F)
Edited by DLANOD B. KCIMROCCM DNA LEUMEL D. THGIRW
EMULOV 68. Recombinant DNA
Edited by YAR Wu
EMULOV 69. Photosynthesis and Nitrogen Fixation (Part C)
Edited yb YNOHTNA NAS ORTEIP
EMULOV 70. Immunochemical Techniques (Part A)
Edited yb HELEN NAV SIKANUV DNA NHOJ J. ENOGNAL
EMULOV 71. Lipids (Part C)
Edited by M. JOHN NIETSNEWOL
EMULOV .27 Lipids (Part D)
Edited by M. JOHN NIETSNEWOL