Table Of ContentPLASMJ:DS J:N BACTERJ:A
BASIC LIFE SCIENCES
Alexander Hollaender, General Editor
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David A. Jackson, and Alexander Hollaender
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PLAS:M:J:DS J:N' BACTEBJ:A
Edited by
Donald R. Helinski
University of California, San Diego
La Jolla, California
Stan ley N. Cohen
Stanford University
Stanford, California
Don B. Clewell
University of Michigan
Ann Arbor, Michigan
David A. Jackson
Genex Corporation
Gaithersburg, Maryland
Alexander Hollaender
Council for Research Planning
in Biological Sciences, Inc.
Washington, D.C.
with
Lowell Hager, Samuel Kaplan,
and Jordan Konisky
Claire M. Wilson, Technical Editor
Council for Research Planning
in Biological Sciences, Inc.
Washington, D.C.
PLENUM PRESS • NEW YORK AND LONDON
Library of Congress Cataloging in Publication Data
Main entry under title:
Plasm ids in bacteria.
(Basic life sciences; v. 30)
"Proceedings of a conference on plasm ids in bacteria, held May 13-18, 1984, at the Univer
sity of Illinois, Urbana, Illinois" - T.p. verso.
Includes bibliographical references and index.
1. Plasm ids-Congresses. 2. Bacteria-Congresses. I. Helinski, Donald R. II. Series.
QR76.6.P57 1985 589.9'087322 84-26397
ISBN-13: 978-1-4612-9487-0 e-ISBN-13: 978-1-4613-2447-8
DOl: 10.1007/978-1-4613-2447-8
Proceedings of a conference on Plasm ids in Bacteria, held May 13-18, 1984,
at the University of Illinois, Urbana, Illinois
©1985 Plenum Press, New York
Softcover reprint of the hardcover 1s t edition 1985
A Division of Plenum Publishing Corporation
233 Spring Street, New York, N.Y. 10013
All rights reserved
No part of this book may be reproduced, stored in a retrieval system, or transmitted
in any form or by any means, electronic, mechanical, photocopying, microfilming,
recording, or otherwise, without written permission from the Publisher
DEDICATION
The Editorial Committee of the conference on "Plasmids in Bac
teria" dedicates this volume to Dr. I. C. Gunsalus in recognition
and appreciation of his distinguished contributions to the fields of
biochemistry and bacteriology. Gunny's independent scientific
career spans some four decades and includes a number of innovative
and fundamental contributions to our understanding of basic biochem
ical processes and bacterial metabolism. His early biochemical
studies led to the isolation of the coenzymes pyridoxal phosphate
and the discovery of lipoic acid. His later classical studies on
the Pseudomonads provided the foundation for both fundamental and
applied studies on the genetics and biochemistry of alkane and
v
vi DEDICATION
terpene catabolic systems in bacteria. This pioneering research in
cluded the characterization of plasmids as carriers of the genes
specifying the degradative enzymes responsible for these catabolic
pathways. His illustrious career as a biochemist and bacteriologist
has earned him a number of awards and honors. We add this dedica
tion and extend our wishes for his continued success in the applica
tion of his extraordinary research ability to those research pro
jects that currently command his interest.
ACKNOWLEDGMENTS
We gratefully acknowledge the generous support of the National
Science Foundation (Grant NSF PCM 84-06530), the National Cancer
Institute (Grant 1 R13 CA 38747), the Argonne Universities Associa
tion Trust Fund, and the University of Illinois Research Board.
This material was prepared with the support of the U.S. Depart
ment of Energy (Grant DOE DEF G 2-84ER13201). However, any opin
ions, findings, conclusions, or recommendations expressed herein are
those of the author(s) and do not necessarily reflect the views of
DOE.
This work was also supported by the Department of Navy Grant
N00014-84-G-0031 issued by the Office of Naval Research. The United
States Government has a royalty-free license throughout the world in
all copyrightable material contained herein.
Financial support was also derived from a number of industrial
contributors as cited in alphabetical sequence: Abbott Laborator
ies; AMGen; Beckman; Biogen Corporation; CIBA-Geigy; Genex Corpora
tion; International Biotechnologies, Inc.; Koppers Company, Incor
porated; Eli Lilly; Merck, Sharp and Dohme Research Laboratories;
Monsanto; New England Nuclear; Pfizer; Pioneer Hi-Bred; and UpJohn.
vii
CONTENTS
In t roduc t ion ................................................. 1
Stanley N. Cohen and Donald R. Helinski
STRUCTURE AND EVOLUTION
Plasmids as Organisms 3
Naomi Datta
Report on a Workshop: Structure and Function ••••••••••••••••• 17
RUdiger Schmitt
Evolutionary Relevance of Genetic Rearrangements
lnva!v ing Plasmids ..................................... 21
Werner Arber, Christian Sengstag, Patrick Caspers,
and Brian Dalrymple
Mechanisms of Transposition in Bacteria •••••••••••••••••••••• 33
Douglas E. Berg
Insertion of Transcriptional Elements Outside the
Replication Region Can Interfere with
Replication, Maintenance, and Stability of
ColEI-Derived Plasmids ••••.•••••••••••••••••••••••••••• 45
Hermann Bujard, Dietrich Stueber, Reiner Gentz,
Ulrich Deuschle, and Ursula Peschke
Studies on the Transposition of lSI.......................... 53
Michael Chandler and David J. Galas
On the Transposition and Evolution of Tn1721
and its Relatives ...................................... 79
RUdiger Schmitt, Sabine MBtsch, Peter Rogowsky,
Fernando de la Cruz, and John Grinsted
ix
x CONTENTS
Repeated DNA Sequences Recombine 1.000 Times More
Frequently in a Plasmid Than in the
Chromosome of Bacillus Subtilis •••••••••••••••••••••••• 93
L. Janni~re. B. Niaudet. and S.D. Ehrlich
Mercury Resistance Transposon Tn813 Mediates
Chromosome Transfer in RhodOpseudomonas
sphaeroides and Intergeneric Transfer of pBR322 •••••••• 105
A. R. St. G. Bowen and J. M. Pemberton
Report on a Workshop: Plasmids in Unusual Systems •••••••••••• 117
Simon Silver. John Pemberton. and Kenji Sakaguchi
REPLICATION. INCOMPATIBILITY. AND PARTITION
Chairman's Introduction: Replication. Incompatibility.
and Partition .......................................... 119
Kurt NordstrHm
Role of the ~ Initiation Protein and Direct Nucleotide
Sequence Repeats in the Regulation of Plasmid
R6K Replication .................•...................... 125
Marcin Filutowicz. Michael McEachern. Alan Greener.
Pradip Mukhopadhyay. Elliott Uhlenhopp.
Ross Durland. and Donald Helinski
Initiation of Replication of the Escherichia Coli
Chromosomal Origin Reconstituted with ----
Purified Enzymes ....................................... 141
Jon M. Kaguni. LeRoy L. Bertsch. David Bramhill.
James E. Flynn. Robert S. Fuller. Barbara Funnell.
Satoko Maki. Tohru Ogawa. Kazuko Ogawa.
Arie van der Ende. and Arthur Kornberg
Origin and InitiaUon Sites of Adv DNA Replication In Vitro.. 151
Toshiki Tsurimoto. Haruhiko Kouhara. and
Kenichi Matsubara
Broad Host-Range Plasmid Rl162: Replication.
Incompatibility. and Copy-Number Control 173
Richard J. Meyer. Lung-Shen Lin.
Kyunghoon Kim. and Michael A. Brasch
Control of Plasmid Replication: Theoretical Considerations
and Practical Solutions ...•........••.......•.........• 189
Kurt NordstrHm
The Partition Functions of Pl. P7. and F Miniplasmids •••••••• 215
Stuart Austin and Ann Abeles
CONTENTS xi
Genetic Interactions of Broad Host-Range Plasmid RK2:
Evidence for a Complex Replication Regulon ••••••••••••• 227
David H. Figurski, Calvin Young, Helen C. Schreiner,
Robert F. Pohlman, David H. Bechhofer,
Alice S. Pr.ince, and Thomas F. D' Amico
Replication Determinants of the Broad Host
Range Plasmid RSFlOlO •••••••••••••••••••••••••••••••••• 243
Peter Scholz, Volker Haring, Eberhard Scherzinger,
Rudi Lurz, Miroslawa M. Bagdasarian,
Heinz Schuster, and Michael Bagdasarian
Regulation of Replication and Maintenance Functions
of Broad Host-Range Plasmid RK2 •••••••••••••••••••••••• 261
C. M. Thomas, C. A. Smith, V. Shingler, M. A. Cross,
A. A. K. Hussain, and M. Pinkney
Control of Chromosome Replication in Bacteria 277
R. H. Pritchard
Stable Maintenance of Plasmid CLO DF13: Structural and
Functional Relationships Between Replication
Control, Partitioning, and Incompatibility ••••••••••••• 283
H. John J. Nijkamp, Bob van Gemen, Marcel J. J. Hakkaart,
Arnold J.van Putten, and Eduard Veltkamp
Replication Control for PT18l,
An Indirectly Regulated Plasmid •••••••••••••••••••••••• 299
Richard P. Novick, Steven J. Projan,
C. Chandra Kumar, Stephen Carleton,
Alexandra Gruss, Sarah K. Highlander,
and John Kornblum
Construction of ColEl RNAl Mutants and
Analysis of their Function In Vivo ••••••••••••••••••••• 321
Barry Polisky, Joseph Tamm, and Tim Fitzwater
Incompatibility and INCFII Plasmid Replication Control 335
Robert H. Rownd, David D. Womble, Xin-nian Dong,
Verne A. Luckow, and Ru Ping Wu
PI Plasmid Maintenance: A Paradigm of Precise Control •••••••• 355
Dhruba K. Chattoraj, Ann L. Abeles,
and Michael B. Yarmolinsky
Partitioning of the pSClOl Plasmid During Cell Division •••••• 383
Stanley N. Cohen, Christine A. Miller,
William T. Tucker, Peter A. Meacock,
and Peter Gustafsson
