Plant Protoplasts and Genetic Engineering VI

328 Pages·1995·10.236 MB·English

by  Z. Li, R. L. Jarret, J. W. Demski (auth.), Professor Dr. Y. P. S. Bajaj (eds.)

Biotechnology in Agriculture and Forestry
Springer-Verlag Berlin Heidelberg GmbH
Volumes already published
Volume 1: Trees I (1986)
Volume 2: Crops I (1986)
Volume 3: Potato (1987)
Volume 4: Medicinal and Aromatic Plants I (1988)
Volume 5: Trees 11 (1989)
Volume 6: Crops 11 (1988)
Volume 7: Medicinal and Aromatic Plants II (1989)
Volume 8: Plant Protoplasts and Genetic Engineering I (1989)
Volume 9: Plant Protoplasts and Genetic Engineering II (1989)
Volume 10: Legumes and Oilseed Crops I (1990)
Volume 11: Somaclonal Variation in Crop Improvement I (1990)
Volume 12: Haploids in Crop Improvement I (1990)
Volume 13: Wheat (1990)
Volume 14: Rice (1991)
Volume 15: Medicinal and Aromatic Plants III (1991)
Volume 16: Trees 111(1991)
Volume 17: High-Tech and Micropropagation I (1991)
Volume 18: High-Tech and Micropropagation II (1992)
Volume 19: High-Tech and Micropropagation III (1992)
Volume 20: High-Tech and Micropropagation IV (1992)
Volume 21: Medicinal and Aromatic Plants IV (1993)
Volume 22: Plant Protoplasts and Genetic Engineering III (1993)
Volume 23: Plant Protoplasts and Genetic Engineering IV (1993)
Volume 24: Medicinal and Aromatic Plants V (1993)
Volume 25: Maize (1994)
Volume 26: Medicinal and Aromatic Plants VI (1994)
Volume 27: Somatic Hybridization in Crop Improvement I (1994)
Volume 28: Medicinal and Aromatic Plants VII (1994)
Volume 29: Plant Protoplasts and Genetic Engineering V (1994)
Volume 30: Somatic Embryogenesis and Synthetic Seed I (1995)
Volume 31: Somatic Embryogenesis and Synthetic Seed II (1995)
Volume 32: Cryopreservation of Plant Germplasm I (1995)
Volume 33: Medicinal and Aromatic Plants VIII (1995)
Volume 34: Plant Protoplasts and Genetic Engineering VI (1995)
Volumes in preparation
Volume 35: Trees IV
Volume 36: Somaclonal Variation in Crop Improvement II
Volume 37: Medicinal and Aromatic Plants IX
Volume 38: Plant Protoplasts and Genetic Engineering VII
Volume 39: High-Tech and Micropropagation V
Volume 40: High-Tech and Micropropagation VI
Biotechnology in
Agriculture and Forestry 34
Plant Protoplasts
and Genetic Engineering VI
Edited by Y.P.S. Bajaj
With 103 Figures and 58 Tables
Professor Dr. Y.P.S. BAJAJ
A-137
New Friends Colony
New Delhi 110065, India
ISBN 978-3-642-63374-4
CIP data applied for
Die Deutsche Bibliothek - CIP-Einheitsaufnahme
Plant protoplasts and genetic engineering. - Berlin; Heidelberg; New York; London; Paris; Tokyo;
Hong Kong; Barcelona; Budapest: Springer.
6 (1995)
(Biotechnology in agriculture and forestry; Vol.34)
ISBN 978-3-642-63374-4 ISBN 978-3-642-57840-3 (eBook)
DOI 10.1007/978-3-642-57840-3
NE: GT
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© Springer-Verlag Berlin Heidelberg 1995
Originally published by Springer-Verlag Berlin Heidelberg New York
Softcover reprint of the hardcover 1st edition 1995
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Dedicated to my friend
Dr. Mike Davey with whom I collaborated
in protoplast culture work
at the University of Nottingham, England,
from 1972-1973
Preface
Plant protoplasts have proved to be an excellent tool for in vitro manipu
lations, somatic hybridization, DNA uptake and genetic transformation,
and for the induction of somaclonal variation. These studies reflect the far
reaching impact of protoplast alterations for agriculture and forest bio
technology. Taking these aspects into consideration, the series of books on
Plant Protoplasts and Genetic Engineering provides a survey of the litera
ture, focusing on recent information and the state of the art in protoplast
manipulation and genetic transformation. This book, Plant Protoplasts
and Genetic Engineering VI, like the previous five volumes published in
1989,1993, and 1994, is unique in its approach. It comprises 27 chapters
dealing with the regeneration of plants from protoplasts, and genetic
transformation in various species of Arachis, Bupleurum, Capsella,
Dendrobium, Dianthus, Diospyros, Fagopyrum, Festuca, Gentiana,
Glycyrrhiza, Gossypium, Hemerocallis, Levisticum, Lonicera, Musa,
Physallis, Platanus, Prunus, Saposhnikovia, Solanum, Spinacia, Trititrigia,
Tulipa, and Vaccinium; including fruits such as apricot, banana, cranberry,
pepino, peach, and plum. This book may be of special interest to advanced
students, teachers, and research scientists in the field of plant tissue culture,
molecular biology, genetic engineering, plant breeding, and general bio
technology.
New Delhi, August 1995 Professor Y.P.S. BAJA]
Series Editor
Contents
Section I Regeneration of Plants from Protoplasts
1.1 Regeneration of Plants from Protop1asts
of Arachis Species (Peanut)
Z. LI, R.L. JARRET, and J.W. DEMSKI (With 2 Figures)
1 Introduction ......................................... 3
2 Isolation of Prot opl asts ................................ 4
3 Culture of Protoplasts ................................. 5
4 Regeneration of Plants ................................. 6
5 Fusion of Protoplasts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
6 Protocol ............................................ 8
7 Summary and Conclusions ............................. 11
References ............................................ 12
1.2 Regeneration of Plants from Protoplasts
of Bupleurum scorzonerifolium (Red Thorowax)
and Saposhnikovia divaricata (Divaricate Saposhnikovia)
H.M. CHEN, G.M. XIA, and S.H. SHENG (With 2 Figures)
1 Introduction .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
2 Bupleurum scorzonerifolium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
3 Saposhnikovia divaricata ............................... 19
References ............................................ 23
1.3 Regeneration of Plants from Protoplasts
of Capsel/a bursa-pastoris (L.) Medic. (Shepherd's Purse)
A.-c. BONFILS, S.C. GLEDDlE, and W.A. KELLER (With 10 Figures)
I Introduction ... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
2 Plant Material ....................................... 26
3 Isolation of Prot opl asts ................................ 26
4 Culture of Protoplasts ................................. 27
5 Somatic Embryogenesis and Plant Regeneration . . . . . . . . . . . . 27
6 Conclusions ......................................... 29
7 Protocol ............................................ 29
References ............................................ 31
x Contents
1.4 Regeneration of Plants from Protoplasts
of Dianthus Species (Carnation)
M. NAKANO and M. Mil (With I Figure)
I Introduction ......................................... 33
2 Protoplast Isolation ................................... 34
3 Protoplast Culture .................................... 35
4 Plant Regeneration ................................... 38
5 Somatic Hybridization and Genetic Transformation. . . . . . . . . 39
6 Summary ........................................... 40
7 Protocol ............................................ 40
References ............................................ 41
1.5 Regeneration of Plants from Protoplasts
of Diospyros kaki L. (Japanese Persimmon)
M. TAMURA, R. TAO, and A. SUGIURA (With 10 Figures)
I Introduction ......................................... 43
2 Isolation of Protoplasts ................................ 44
3 Culture of Protoplasts ................................. 46
4 Regeneration of Plants ................................ 50
5 Fusion of Protoplasts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 I
6 Summary and Conclusions ............................. 52
7 Protocol ............................................ 52
References ............................................ 54
1.6 Regeneration of Plants from Protoplasts
of Gentiana Species (Gentian)
Y. TAKAHATA, H. JOMOR!, S. MIYANO, H. KUNITAKE,
and M. MIl (With I Figure)
I Introduction ......................................... 55
2 Isolation of Protoplasts ................................ 56
3 Culture of Protoplasts ................................. 57
4 Regeneration of Plants ................................ 59
5 Fusion of Protopl~sts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
6 Conclusions ......................................... 60
7 Protocol ............................................ 60
References ............................................ 6 I
1.7 Regeneration of Plants from Protoplasts
of Gossypiul11 Species (Cotton)
J.M. SHE, J.Y. Wu, and H.Y. ZHOU (With I Figure)
I Introduction ......................................... 63
2 Isolation of Protoplasts ................................ 64
3 Culture of Protoplasts ................................. 65
4 Regeneration of Plants ................................ 66
Contents XI
5 Summary 68
6 Protocol 68
References ........................................... . 68
1.8 Regeneration of Plants from Protoplasts
of Hemerocaflis (Daylily)
A.D. KRIKORIAN (With 2 Figures)
I Introduction ......................................... 70
2 Protocols. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
3 Conclusion .......................................... 76
References ............................................ 76
1.9 Regeneration of Plants from Protoplasts
of Levisticum officinale Koch (Lovage)
S.S. Guo (With I Figure)
I Introduction ........ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
2 Isolation of Protoplasts ................................ 80
3 Culture of Protoplasts ................................. 83
4 Regeneration of Plants ................................ 86
5 Fusion of Protoplasts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
6 Summary ........................................... 87
7 Protocol ............................................ 87
References ............................................ 88
I.l 0 Regeneration of Plants from Protoplasts
of Lonicera Species (Honeysuckle)
S.l. OCHATT and D. GEORGES (With I Figure)
I Introduction ........ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
2 Background on Tissue Culture Studies with
Lonicera Genotypes .......... . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
3 Protoplast Studies with Lonicera ........................ 91
4 Conclusion .......................................... 99
References ............................................ 99
I.lI Regeneration of Plants from Protoplasts
of Platanus orientalis (Plane Tree)
Z.M. WEI and Z.H. Xu (With I Figure)
I Introduction .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101
2 Establishment of Sterile Shoot Culture . . . . . . . . . . . . . . . . . . . . 102
3 Protoplast Isolation and Culture ........................ 102
4 Callus Proliferation and Plant Regeneration
from Protoplast-Derived Calli ............................ 104
5 Summary ........................................... 106
6 Protocol ............................................ 106
References ............................................ 107
XII Contents
1.12 Regeneration of Plants from Protoplasts
of Solanum khasianum C.B. Clark and Solanum laciniatum Ait.
D. SIHACHAKR, I. SERRAF, M.H. CHAPUT, 1. MUSSIO,
L. ROSSIGNOL, and G. DUCREUX (With I Figure)
I Introduction .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 108
2 Isolation of Protoplasts ................................ III
3 Culture of Protoplasts ................................. 113
4 Plant Regeneration from Protoplast-Derived Callus. . . . . . . . . 115
5 Fusion of Protoplasts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 116
6 Summary and Conclusion .............................. 117
7 Protocol ............................................ 117
References ............................................ 118
1.13 Regeneration of Plants from Pro top lasts
of Trititrigia (Triticum sect. trititrigia)
T.B. WANG and Y.Q. QIAN (With 4 Figures)
Introduction ... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121
2 Establishment of Embryogenic Cell Suspensions .... . . . . . . . . 122
3 Isolation,of Protoplasts ................................ 123
4 Culture of Protoplasts ................................. 126
5 Regeneration of Plants ................................ 127
6 Fusion of Protoplasts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 128
7 Conclusions and Prospects ............................. 129
8 Experimental Protocol. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 129
References ............................................ 130
Section II Genetic Transformation and Transgenic Plants
II.l Genetic Transformation in Arachis hypogaea L. (Peanut)
J.A. SCHNALL and A.K. WEISSINGER (With 6 Figures)
1 Introduction......................................... 135
2 Transformation ...................................... 136
3 Conclusions ......................................... 143
References ............................................ 143
11.2 Genetic Transformation in Dendrobium (Orchid)
G.-L. NAN and A.R. KUEHNLE (With 6 Figures)
1 Introduction ........... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 145
2 Genetic Engineering of Dendrobium ...................... 146
3 Summary ........................................... 153
References ............................................ 154