Table Of ContentPHOSPHATE AND
MINERAL METABOLISM
ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY
Editorial Board:
NATHAN BACK, State University of New York at Buffalo
NICHOLAS R. DI LUZIO, Tulane University School of Medicine
EPHRAIM KATCHALSKI-KATZIR, The Weizmann Institute of Science
DAVID KRITCHEVSKY, Wistar Institute
ABEL LAJTHA, Rockland Research Institute
ROOOLFO PAOLETTI, University of Milan
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PHOSPHATE AND MINERAL METABOLISM
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PHOSPHATE AND
MINERAL METABOLISM
Edited by
Shaul G. Massry
University of Southern California
Los Angeles, California
Giuseppe Maschio
University of Verona
Verona, Italy
and
Eberhard Ritz
University of Heidelberg
Heidelberg, Federal Republic of Germany
PLENUM PRESS • NEW YORK AND LONDON
Library of Congress Cataloging in Publication Data
International Workshop on Phosphate and Other Minerals (6th: 1983: Verona, Italy)
Phosphate and mineral metabolism.
(Advances in experimental medicine and biology; v. 178)
"Proceedings of the Sixth International Workshop on Phosphate and Other Minerals,
held June 24-26,1983, in Verona, Italy"-T.p. verso.
Bibliography: p.
Includes index.
1. Mineral metabolism - Congresses. 2. Phosphates - Metabolism - Congresses. 3.
Mineral metabolism - Disorders - Congresses. I. Massry, Shaul G.II. Maschio,
Giuseppe. III. Ritz, Eberhard. IV. Title. V. Series.
QP533.I58 1983 599'.0133 84-13450
ISBN-13: 978-1-4684-4810-8 e-ISBN-13: 978-1-4684-4808-5
DOl: 10.1007/978-1-4684-4808-5
Proceedings of the Sixth International Workshop on Phosphate and
other Minerals, held June 24-26, 1983, in Verona, Italy
©1984 Plenum Press, New York
Softcover reprint of the hardcover 1s t edition 1984
A Division of Plenum Publishing Corporation
233 Spring Street, New York, N.Y. 10013
All rights reserved
No part of this book may be reproduced, stored in a retrieval system, or transmitted
in any form or by any means, electronic, mechanical, photocopying, microfilming,
recording, or otherwise, without written permission from the Publisher
TO OUR WIVES
Meira Massry
Emilia Maschio
Christina Ritz
AND TO OUR CHILDREN
PREFACE
We are pleased to present to our readers the Proceedings of
the Sixth International Workshop on Phosphate and Other Minerals
which was held in Verona, Italy, during June 24-26, 1983. It was
hosted by Professor Giuseppe Maschio, Professor of Medicine and
Chief, Department of Nephrology at the University of Verona.
The Sixth Workshop maintained the tradition of the previous
ones. It provided a unique and outstanding opportunity for close
interaction between scientist involved in the research of the
overall field of Mineral Metabolism. The current Workshop was
attended by 250 scientists from 15 countries including Austria,
Canada, Denmark, England, France, Germany,Holland, Israel, Italy,
Japan, Spain, Sweden,Switzerland, and the United States of
America. The topics discussed included the renal handling of
phosphate, transport of other minerals, intestinal absorption of
calcium and phosphate and phosphate homeostasis in health and
disease. Two symposia dealing with the recent developments of
the interactions between minerals, parathyroid hormone, and blood
pressure and between minerals and myopathies were included. In
addition to the 15 State-of-the-Art Lectures, there were 43 oral
and 63 poster presentations selected from over 200 abstracts sub
mitted to the Program Committee.
The Seventh International Workshop on Phosphate and Other
Minerals will be held during September, 1985, in Marseille, France.
It will be hosted by Professor Michel almer, Chief of the
Department of Nephrology at the University of Marseille. The
theme of this coming Workshop will focus on the pathophysiology
of phosphate homeostasis and the metabolism of other minerals.
We would like to express our thanks and appreciation for all
those who have stimulated, encouraged, and supported us to hold
the Sixth International Workshop in Verona. The meeting could not
have been possible without the generous financial support of the
City of Verona and Cassa Di Respamio Di Verona, Italy, Hoffman
La Roche (USA), Plenum Publishing Company (USA), Procter and
Gamble (USA), Sandoz (Italy), Veneto Regional Government (Italy),
vii
and the University of Verona (Italy). We would like to thank
Ms. Joann Little for her secretarial help and Mrs. Haidy Samouha
for her contributions in the organization of the Workshop.
Shaul G. Massry
Giuseppe Maschio
Eberhard Ritz
viii
CONTENTS
RENAL HANDLING OF PHOSPHATE
The Biochemical Modifications of the Brush Border
Membrane Induced by Vitamin D and Parathyroid
Hormone in Their Actions on Phosphate Transport. 3
K.A. Hruska, B. Kurnik, and M. Tsutsumi
Intracellular Processes that Affect Renal Phosphate
Transport. . • • • • • • • • • • • • • • • • • 21
V.W. Dennis and P.C. Brazy
Effect of Serum Calcium on Phosphate Transport • • • • • • •• 25
W.N. Suki
Influence of Temperature on Phosphate Uptake by Renal
Brush Border Membrane Vesicles • • • • • • • • • 29
M. Gagnan-Brunette and R. B6liveau
Parathyroid Hormone (PTH) Status and Phosphate (Pi)
Transport in Proximal Brush Border Membrane
Vesicles (BBMV) and Whole Kidney • • • • • • • 33
J. Caverzasio and J.P. Bonjour
Renal Brush Border Membranes in X-Linked Hypophosphatemic
Mice: A Study of the Protein Kinase Activity • • • • 37
M. Gagnan-Brunette and R. B~liveau
Biochemical Studies of the Control of Renal Tubular
Phosphate Reabsorption • • • • • • • • • • 41
M.F. Grahn, R. Parveen, and P.J. Butterworth
Role of c-AMP and NAD in the Regulation of Na+ - Dependent
Phosphate Transport Across Rat Renal Brush Border
Membranes. • • • • • • • • • • • • • 49
J. Biber, P. Gmaj, K. Malmstrom, and H. Murer
Gluconeogenesis, Cytosolic Redox Potential, and Phosphate
and Fluid Transport in the Isolated Rabbit Proximal
Tubule . • • • • • . . .• • • • • • • • . • . • 59
N. Yanagawa, G.T. Nagami, and K. Kurokawa
ix
Comparison of the Effect of Nucleotides on Phosphate
Transport in Renal Brush Border Membrane
Vesicles and Intact Proximal Tubules •••• 69
L.G. Fine. R. Lang. E.P. Nord. and N. Yanagawa
The Usefulness of Isolated Renal Cortical Cells to
Study Phosphate Transport ••••• • 73
N. Tessitore. L.M. Sakhrani. and S.G. Massry
Calcium eCa) Mediated Phosphate (P04) Transport in the
Proximal Convoluted Tubule (PCT) of the Rabbit. • 77
D. Rouse and W.N. Suki
Glucocorticoid-Induced Inhibition of the Reabsorption
of Inorganic Phosphate in the Proximal Tubule in
the Absence of Parathyroid Hormone. • • • • • • • 81
A. Frick. I. Durasin. and M. Neuweg
Effect of 1.25-Dihydroxycholecalciferol on the Extrarenal
Handling of Inorganic Phosphate in
Thyroparathyroidectomized but not in Intact Rats. • 87
U. Troeh1er. J.P. Bonjour. and H. Fleisch
Early Renal Adaptation to Dietary Phosphorus Restriction. 93
B.S. Levine. K. Kurokawa. and J.W. Coburn
Phosphaturia of Aging: Studies on Mechanisms. • • • • • 103
D.B.N. Lee, N. Yanagawa, O. Jo, B.P. Yu, and N. Beck
CALCIUM AND MAGNESIUM TRANSPORT
The Assessment of Renal Calcium Reabsorption 111
O.L.M. Bijvoet and H.I.J. Harinck
Cis-P1atin Induced Hypermagnesiuria and Hyperca1ciuria in
Rats. . • • • • . . . . . • 127
V. Mavichak, N.L.M. Wong, J.H. Dirks, and
R.A.L. Sutton
Brush Border Vesicle Transport Effects of 1,25-Dihydroxy
Vi tamin D3. • • • • • . • • • • . • • • . . • . 133
J.B. Puschett. J. Ege1. and J. pfanstie1
Active Calcium Transport in Frog Skin: Stimulation by
Cyclic AMP. • • • • • • • • • • • • • • • • • • •• 135
E. Kelepouris. M. Civan. and Z.S. Agus
x
ATP as a Factor in the Response of the cAMP System to
PTH in Proximal and Distal Convoluted Tubules •• 139
T.P. Dousa, G.M. Kiebzak, A.N.K. Yusufi, E. Kusano,
and J. Braun-Werness
Different Effects of Parathyroid Hormone on Epithelial
Transport. • • • • • • • • • • • • • • • • • 145
S. Sabatini, R. Mola, J.T. McCreary, and N.A. Kurtzman
INTESTINAL ABSORPTION OF PHOSPHATE AND CALCIUM
Verapamil Improves Defective Intestinal Calcium Absorption
in Uremia. • • • . . . . • • • • • • • • • . • • •. 153
M.S. Goligorsky, C. Chaimovitz, She Shany, J. Rapoport,
and Y. Sharony
Vitamin D Activates (Na+ -K+) ATPase: A Possible
Regulation of Phosphate and Calcium Uptake by
Cultured Embryonic Chick Small Intestine ••• 163
H.S. Cross and M. Peterlik
Effects of pH and Sodium on Phosphate Transport Across
Brush Border Membrane Vesicles of Small Intestine. 173
G. Danisi, H. Murer, and R.W. Straub
Direct In Vitro Effects of 1,25(OH)2 Vitamin D3 on
Phosphate Transport in Isolated Enterocytes from
Normal or Vitamin D Deficient Rats • • • • • • • • 181
G. Karsenty, B. Lacour, A. Ulmann, E. Pierandrei and
T. Drueke
1,25-Dihydroxyvitamin D3 Stimulates Calcium and Phosphate
Absorption by Different Mechanisms: Contrasting
Requirements for Sodium • • • • • • • • • • • • • • 189
D.B.N. Lee, M.W. Walling, D.B. Corry, and J.W. Coburn
Dissociation of Absorptions of Calcium and Phosphate
in Different Pathophysiological States in Man. • 195
C. Gennari, M. Bernini, P. Nardi, and L. Fusi
TOPICS ON BONE
Bone Remodelling After Renal Transplantation (RT) • • • • • • 207
V. Bonomini, C. Feletti, A. Di Felice, and
A. Buscaroli
Inorganic Phosphate, Pyrophosphate and the Diphosphonates
Activate Bone (Calvaria) Glutaminase. • • • • • • • 217
R.M. Biltz, E.D. Pellegrino, J.M. Letteri, and
L.M. Pinkus
xi
