Table Of ContentAcademic Press Rapid Manuscript Reproduction
Based on the Conference "A Century Since Metchnikoff:
Phagocytosis—Past and Future" Held in the Province of
Messina, Sicily, Italy, September 22-27, 1980
Phagocytosis—Past and Future
Edited by
Manfred L. Karnovsky
Department of Biological Chemistry
Harvard Medical School
Boston. Massachusetts
Liana Bolis
Department of Comparative Physiology
University of Messina
Sicily, Italy
1982
ACADEMIC PRESS
A Subsidiary of Harcourt Brace Jovanovich, Publishers
New York London
Paris San Diego San Francisco Säo Paulo Sydney Tokyo Toronto
COPYRIGHT © 1982, BY ACADEMIC PRESS, INC.
ALL RIGHTS RESERVED.
NO PART OF THIS PUBLICATION MAY BE REPRODUCED OR
TRANSMITTED IN ANY FORM OR BY ANY MEANS, ELECTRONIC
OR MECHANICAL, INCLUDING PHOTOCOPY, RECORDING, OR ANY
INFORMATION STORAGE AND RETRIEVAL SYSTEM, WITHOUT
PERMISSION IN WRITING FROM THE PUBLISHER.
ACADEMIC PRESS, INC.
Ill Fifth Avenue, New York, New York 10003
United Kingdom Edition published by
ACADEMIC PRESS, INC. (LONDON) LTD.
24/28 Oval Road, London NW1 7DX
Library of Congress Cataloging in Publication Data
Main entry under title:
Phagocytosis—past and future.
Outgrowth of a symposium held at the University of
Messina, Sept. 22-27, 1980.
Bibliography: p.
Includes index.
1. Phagocytosis—Congresses. I. Karnovsky, Manfred,
Date. II. Bolis, Liana. [DNLM: 1. Phagocytosis.
QW 690 P534]
QR187.P4P49 1982 616.07'9 82-11461
ISBN 0-12-400050-9
PRINTED IN THE UNITED STATES OF AMERICA
82 83 84 85 9 8 7 6 5 4 3 2 1
Contributors
Numbers in parentheses indicate the pages on which the authors' contributions begin.
Jon S. Abramson1 (463), Department of Pediatrics, University of Minnesota
Medical School, Minneapolis, Minnesota 55455
S. K. Adeniyi-Jones2 (193), Department of Biological Chemistry, Harvard
Medical School, Boston, Massachusetts 02115
M. Aellig (149), Laboratoire Central de Chemie Clinique, Centre Hospitalier
Universitaire Vaudois (CHUV), CH-1011 Lausanne, Switzerland
Β. A. Askonas (505), Division of Immunology, National Institute for Medical
Research, The Ridgeway, Mill Hill, London NW71AA, England
Bernard M. Babior (157), Hematology Service, Department of Medicine, Tufts
University School of Medicine, and New England Medical Center Hospital,
Boston, Massachusetts 02111
John A. Badwey (193, 419), Department of Biological Chemistry, Harvard
Medical School, Boston, Massachusetts 02115
M. Baggiolini (339), Wander Research Institute, Wander Ltd., CH-3001 Berne,
Switzerland
Frederik Β. Bang3 (567), Department of Pathobiology, School of Hygiene and
Public Health, Johns Hopkins University, Baltimore, Maryland 21205, and
Marine Biological Laboratory, Woods Hole, Massachusetts 02543
Zvi Bar-Shavit (259), Department of Membrane Research, Weizmann Institute
of Science, Rehovot, Israel
Elmer L. Becker (87), Department of Pathology, University of Connecticut
Health Center, Farmington, Connecticut 06032
P. Bellavite (167), Istituto di Patologia Generale, Facolta di Medicina e
Chirurgia, Universita di Padova-Sede di Verona, Verona, Italy
'Present address: Department of Pediatrics, Bowman-Gray School of
Medicine, Winston-Salem, North Carolina 27103.
2Present address: National Institutes of Health, Bethesda, Maryland 20205.
3Deceased.
ix
χ Contributors
G. Berton (167), Istituto di Patologia Generale, Facoltâ di Mediana e
Chirurgia, Universitâ di Padova-Sede di Verona, Verona, Italy
J. Thomas Blake (239), Department of Immunology, Merck Sharp & Dohme
Research Laboratories, Rahway, New Jersey 07065
Robert W. Bonser (131), Molecular Biology Department, The Wellcome
Research Laboratories, Burroughs Wellcome Company, Research Triangle
Park, North Carolina 27709
James A. Braatz (323), Laboratory of Immunodiagnosis, National Cancer In-
stitute, National Institutes of Health, Bethesda, Maryland 20205
Richard T. Briggs (215), Department of Biological Sciences, Smith College,
Northampton, Massachusetts 01063,
George Cianciolo (67), Division of Rheumatic and Genetic Diseases, Duke
University Medical Center, Durham, North Carolina 27710
Charles G. Cochrane (543), Department of Immunopathology, Scripps Clinic
and Research Foundation, La Jolla, California 92037
B. Dewald (339), Wander Research Institute, Wander Ltd., CH-3001 Berne,
Switzerland
David Drath4 (419), Department of Biological Chemistry, Harvard Medical
School, Boston, Massachusetts 02115
Robert M. Fauve (295), Unité d'Immunophysiologie Cellulaire, Institut
Pasteur, 75724 Paris Cedex 15, France
Douglas T. Fearon (375), Department of Medicine, Harvard Medical School,
Boston, Massachusetts 02115
Elisabeth Fontan (295), Unité d'Immunophysiologie Cellulaire, Institut
Pasteur, 75724 Paris Cedex 15, France
Richard J. Freer5 (87), Department of Pharmacology, University of Connecticut
Health Center, Farmington, Connecticut 06032
J. Frei (149), Laboratoire Central de Chemie Clinique, Centre Hospitalier
Universitaire Vaudois (CHUV), CH-1011 Lausanne, Switzerland
Elaine Κ. Gallin (29), Physiology Department, Armed Forces Radiology
Research Institute, Bethesda, Maryland 20014
John I. Gallin (519), Bacterial Diseases Section, Laboratory of Clinical Investiga-
tion, National Institute of Allergy and Infectious Diseases, National In-
stitutes of Health, Bethesda, Maryland 20205
Rachel Goldman (259), Department of Membrane Research, Weizmann Institute
of Science, Rehovot, Israel
Siamon Gordon (287), Sir William Dunn School of Pathology, University of
Oxford, South Parks Road, Oxford OX1 3RE, England
Otto Götze (357), Abteilung für Immunologie, Zentrum für Hygiene
und Humangenetik der Universität, 3400 Göttingen, Federal Republic of
Germany
4Present address: University of Texas Medical School, Houston, Texas 77030.
'Present address: Department of Pharmacology, Medical College of Virginia,
Richmond, Virginia 23298.
Contributors xi
C. M. Grosskinsky (505), Division of Immunology, National Institute for
Medical Research, The Ridgeway, Mill Hill, London NW71AA, England
P. D'Arcy Hart (437), Medical Research Council, National Institute for Medical
Research, The Ridgeway, Mill Hill, London NW71AA, England
W. R. Henderson (449), Department of Medicine, School of Medicine, Universi-
ty of Washington, Seattle, Washington 98195
Ronald B. Herberman (323), Laboratory of Immunodiagnosis, National Cancer
Institute, National Institutes of Health, Bethesda, Maryland 20205
Brigitte Hevin (295), Unité d'Immunophysiologie Cellulaire, Institut Pasteur,
75724 Paris Cedex 15, France
G. A. Higgs (105), Department of Prostaglandin Research, The Wellcome
Research Laboratories, Langley Court, Beckenham, Kent BR3 3BS,
England
Beate I. Hirsch (1), Department of Graduate Studies, The Rockefeller Univer-
sity, New York, New York 10021
James G. Hirsch6 (1), Department of Graduate Studies, The Rockefeller
University, New York, New York 10021
S. T. Hoffstein (47), Department of Medicine, New York University Medical
Center, New York, New York 10016
A. Jörg (449), Department of Medicine, School of Medicine, University of
Washington, Seattle, Washington 98195
C. Michael Jones7 (323), Laboratory of Immunodiagnosis, National Cancer
Institute, National Institutes of Health, Bethesda, Maryland 20205
E. C. Jong (449), Department of Medicine, School of Medicine, University of
Washington, Seattle, Washington 98195
Manfred L. Karnovsky (193, 215, 419), Department of Biological Chemistry,
Harvard Medical School, Boston, Massachusetts 02115
Morris J. Karnovsky (193, 215), Department of Pathology, Harvard Medical
School, Boston, Massachusetts 02115
S. J. Klebanoff (449), Department of Medicine, School of Medicine, University
of Washington, Seattle, Washington 98195
Η. M. Korchak (47), Department of Medicine, New York University Medical
Center, New York, New York 10016
Janis Lazdins8 (419), Department of Biological Chemistry, Harvard Medical
School, Boston, Massachusetts 02115
P. C. J. Leijh (429), Department of Infectious Diseases, University Hospital,
2333 AA Leiden, The Netherlands
Edward J. Leonard (475), Department of Immunology, Walter Reed Army In-
stitute of Research, Washington, D.C. 20012
S. H. Liu (553), Department of Ophthalmology, The Wilmer Institute, Johns
Hopkins University School of Medicine, Baltimore, Maryland 21205
6Present address: Josiah Macy Jr. Foundation, New York, New York 10020.
7Present address: University of Texas at Houston, Houston, Texas 77030.
8Present address: Pfizer, Inc., Groton, Connecticut 06340.
xii Contributors
R. M. Locksley (449), Department of Medicine, School of Medicine, University
of Washington, Seattle, Washington 98195
G. A. Lutty (553), Department of Ophthalmology, The Wilmer Institute, Johns
Hopkins University School of Medicine, Baltimore, Maryland 21205
M. Maillard (149), Laboratoire Central de Chemie Clinique, Centre Hospitalier
Universitaire Vaudois (CHUV), CH-1011 Lausanne, Switzerland
M. Markert (149), Laboratoire Central de Chemie Clinique, Centre Hospitalier
Universitaire Vaudois (CHUV), CH-1011 Lausanne, Switzerland
Monte S. Meitzer (475), Laboratory of Immunology, National Cancer Institute,
National Institutes of Health, Bethesda, Maryland 20205
Elaine L. Mills9 (463), Department of Pediatrics, University of Minnesota
Medical School, Minneapolis, Minnesota 55455
Paul H. Naccache (87), Department of Pathology, University of Connecticut
Health Center, Farmington, Connecticut 06032
Carol A. Nacy (475), Department of Immunology, Walter Reed Army Institute
of Research, Washington, D.C. 20012
Carl F. Nathan (391), The Rockefeller University, New York, New York
10021
A. Pätz (553), Department of Ophthalmology, The Wilmer Institute, Johns
Hopkins University School of Medicine, Baltimore, Maryland 21205
Marilyn C. Pike (67), Division of Rheumatic and Genetic Diseases, Duke Univer-
sity Medical Center, Durham, North Carolina 27710
R. A. Prendergast (553), Department of Ophthalmology, The Wilmer Institute,
Johns Hopkins University School of Medicine, Baltimore, Maryland 21205
Paul Q. Quie (463), Department of Pediatrics and Laboratory of Medical
Pathology and Microbiology, University of Minnesota Medical School,
Minneapolis, Minnesota 55455
P. G. Ramsey (449), Department of Medicine, School of Medicine, University of
Washington, Seattle, Washington 98195
John M. Robinson (193, 215), Department of Pathology, Harvard Medical
School, Boston, Massachusetts 02115
Alan S. Rosenthal (239), Department of Immunology, Merck Sharp & Dohme
Research Laboratories, Rah way, New Jersey 07065
F. Rossi (167), Istituto di Patologia Generale, Facolta di Mediana e
Chirurgia, Universita di Padova-Sede di Verona, Verona, Italy
D. L. Sacks (505), Division of Immunology, National Institute for Medical
Research, The Ridgeway, Mill Hill, London NW71AA, England
J. Schnyder (339), Wander Research Institute, Wander Ltd., CH-3001 Berne,
Switzerland
Ramadan I. Sha'afi (87), Department of Physiology, University of Connecticut
Health Center, Farmington, Connecticut 06032
'Present address: Department of Pediatrics, McGill University, and Montreal
Children's Hospital, Montreal PQ H3H1P3, Canada.
Contributors xiii
Henry J. Showell10 (87), Department of Pathology, University of Connecticut
Health Center, Farmington, Connecticut 06032
Marvin I. Siegel (131), Molecular Biology Department, The Wellcome Research
Laboratories, Burroughs Wellcome Company, Research Triangle Park,
North Carolina 27709
J. E. Smolen (47), Department of Medicine, New York University Medical
Center, New York, New York 10016
Ralph Snyderman (67), Division of Rheumatic and Genetic Diseases, Duke
University Medical Center, Durham, North Carolina 27710
Roy J. Soberman11 (419), Department of Biological Chemistry, Harvard Medical
School, Boston, Massachusetts 02115
Thomas P. Stossel (13), Hematology-Oncology Unit, Massachusetts General
Hospital, and Department of Medicine, Harvard Medical School, Boston,
Massachusetts 02114
John S. Sundsmo (357), Department of Molecular Immunology, Research In-
stitute of Scripps Clinic, La Jolla, California 92037
James W. Thomas12 (239), Department of Immunology, Merck Sharp & Dohme
Research Laboratories, P.O. Box 2000, Rahway, New Jersey 07065
D. C. Thompson (553), Department of Ophthalmology, The Wilmer Institute,
Johns Hopkins University School of Medicine, Baltimore, Maryland 21205
Ronald W. Walenga13 (87), Department of Pharmacology, University of Connec-
ticut Health Center, Farmington, Connecticut 06032
G. Weissmann (47), Department of Medicine, New York University Medical
Center, New York, New York 10016
Masonori Yanai14 (463), University of Minnesota Medical School, Minneapolis,
Minnesota 55455
Helen L. Yin (13), Hematology-Oncology Unit, Massachusetts General Hospital,
and Department of Medicine, Harvard Medical School, Boston,
Massachusetts 02114
R. van Furth (429), Department of Internal Medicine and Infectious Diseases,
Academisch Ziekenhuis Leiden, Rijnsburgerweg 10, 2333 AA Leiden, The
Netherlands
10Present address: Pfizer Central Research, Pfizer, Inc., Groton, Connecticut
06340.
"Present address: Department of Medicine, Harvard Medical School.
12Present address: Department of Pathology, The Jewish Hospital of St. Louis,
St. Louis, Missouri 63110.
,3Present address: Department of Pharmacology, School of Medicine, Univer-
sity of Syracuse, Syracuse, New York 13210.
14Present address: Nagasaki University Hospital, Nagasaki City, Nagasaki 852,
Japan.
Preface
The functions of phagocytic leukocytes in the host-parasite relationship are
well understood in 1982, as we hope this volume attests. A hundred years ago
those functions had not begun to be appreciated. It was between 1879 and 1882
that Metchnikoff, working in Messina, "discovered" phagocytosis, or, better,
formulated for the first time, in a dramatic and forceful way, the concept of a role
for "wandering" cells in the control of infection and injury.
In October 1980, fifty investigators met in the Province of Messina, Sicily, to
examine the current status of the field whose explosive development Metchnikoff
had triggered a century before. The topics discussed were largely in the areas of
cell biology, cellular immunology, and biochemistry. They ranged from aspects
of cellular movement and the ingestion process per se, to definition of substances
that affect the function of phagocytic leukocytes, and to the nature of cidal
mechanisms at the molecular level. Though at first blush this suggests a very
broad coverage of the field, the editors and contributors all recognize that a
wealth of matters, mainly more clinically linked, remain to be surveyed.
The organizers of, and participants in, the meeting in Messina—who are the
editors of, and contributors to this book — ensured that the observance of the
centennial was not merely ceremonial. Taking stock of a field is often a valuable
exercise. It can be productive, too, in preparing the springboard for the next ven-
tures.
We are deeply grateful for help received from many quarters. The World
Health Organization was the sponsor and the University of Messina was the
academic core of the enterprise. The list of those organizations that helped launch
the meeting and discussions that led to this book is long: Fidia Research
Laboratories (Abano Terme); Lirca Laboratories; Merck Sharp & Dohme Re-
search Laboratories; E. R. Squibb and Sons, Inc.; the Upjohn Company; the Well-
come Research Laboratories; Lederle Laboratories; and the Sterling - Winthrop
Research Institute. Even longer is the list of agencies and institutions in Sicily, and
of individuals, whose efforts made it possible for the scientists to enjoy both their
science and the ambience. To thank them all would require a separate chapter.
XV
xvi Preface
The organization of the topics will seem idiosyncratic to some. It bears little
resemblance to that of the meeting. However, every book with multiple authors
needs editors, and the latter have tried in this instance to fashion a coherent
structure. The individual chapters reflecting the talks that were given were
modified by the discussion and exchange of views in Messina. Each certainly con-
tributes to the field information that is seminal, significant, and salient.
Manfred L. Karnovsky
Liana Bolis
