Table Of ContentOXYGEN TRANSPORT
TO TISSUE-m
ADV ANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY
Editorial Board:
Nathan Back State University of New York at Buffalo
N. R. Di Luzio Tulane University School of Medicine
Bernard Halpern College de France and Institute of Immuno·Biology
Ephraim Katchalski The Weizmann Institute of Science
David Kritchevsky Wistar Institute
Abel Lajtha New York State Research Institute for Neurochemistry and Drug Addiction
Rodolfo Paoletti University of Milan
Recent Volumes in this Series
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PROTEIN CROSSLINKING: Biochemical and Molecular Aspects
Edited by Mendel Friedman
Volume 86B
PROTEIN CROSSLINKING: Nutritional and Medical Consequences
Edited by Mendel Friedman
Volume 87
HYPOTHALAMIC PEPTIDE HORMONES AND PITUITARY REGULATION
Edited by John C. Porter
Volume 88
AVIAN IMMUNOLOGY
Edited by Albert A. Benedict
Volume 89
MUCUS IN HEALTH AND DISEASE
Edited by Max Elstein and Dennis V. Parke
Volume 90
PARKINSON'S DISEASE: Neurophysiological, Clinical, and Related Aspects
Edited by Fathy S. Messiha and Alexander D. Kenny
Volume 91
INORGANIC AND NUTRITIONAL ASPECTS OF CANCER
Edited by G. N. Schrauzer
Volume 92
MORRIS HEPATOMAS: Mechanisms of Regulation
Edited by Harold P. Morris and Wayne E. Criss
Volume 93
IMMUNITY TO BLOOD PARASITES OF ANIMALS AND MAN
Edited by Louis H. Miller, John A. Pino, and John J. McKelvey, Jr .
Volume 94
OXYGEN TRANSPORT TO TISSUE - III
Edited by I. A. Silver, M. Erecinska, and H. I. Bicher
Volume 95
ACID PROTEASES: Structure, Function, and Biology
Edited by Jordan Tang
OXYGEN TRANSPORT
TOTISSUE-m
Edited by
LA. Silver
University of Bristol
Bristol, England
M. Erecinska
University of Pennsylvania
Philadelphia, Pennsylvania
and
H.1. Bicher
Roswell Park Memorial Institute
Buffalo, New York
PLENUM PRESS • NEW YORK AND LONDON
Library of Congress Cataloging in Publication Data
International Symposium on Oxygen Transport to Tissue, 3rd, Churchill College,
1977.
Oxygen transport to tissue, III.
Bibliography: p.
1. Oxygen transport (Physiology)-Congresses. 2. Oximetry-Congresses. 3. Oxygen
in the body-Congresses. I. Silver, I. A. II. Erecmska, M. III. Bicher, "aim I. IV. Title.
[DNLM: 1. Oxygen-Blood-Congresses. 2. Oxygen consumption-Congresses. 3.
Biological Transport-Congresses. W3 IN931BB 3d 1977/QV312 161319770]
QP99.3.09I541977 599'.01'2 77·17140
ISBN 978-1-4684-8892-0 ISBN 978-1-4684-8890-6 (eBook)
001 10.1007/978-1-4684-8890-6
Proceedings of the Third International Symposium
on Oxygen Transport to Tissue held at Churchill College,
Cambridge, England, July 4·7, 1977
© 1978 Plenum Press, New York
Softcover reprint of the hardcover 1st edition 1978
A Division of Plenum Publishing Corporation
227 West 17th Street, New York, N.Y. 10011
All rights reserved
No part of this book may be reproduced, stored in a retrieval system, or transmitted,
in any form or by any means, electronic, mechanical, photocopying, microfilming,
recording, or otherwise, without written permission from the Publisher
INTERNATIONAL SOCIETY
OF
OXYGEN TRANSPORT TO TISSUE
President Dr .. LA. Silver
Bristol, England
President Elect Dr. J. Strauss
Miami, Florida, U.S.A.
Past President Dr. B. Chance
Philadelphia, Pennsylvania, U.S.A.
Secretary Dr. H.I. Bicher
Buffalo, New York, U.S.A.
Treasurer Dr. D.W. Lubbers
Dortmund, Federal Republic of Germany
Members of the International Committee
Dr. D.F. Bruley, Terre Haute, Indiana, U.S.A.
Dr. J. Ditzel, Aarlborg, Denmark
Dr. R.E. Forster, Philadelphia, Pennsylvania, U.S.A.
Dr. J. Grote, Mainz, Federal Republic of Germany
Dr. J. H. Halsey, Birmingham, Alabama, U.S.A.
Dr. C.R. Honig, Rochester, New York, U.S.A.
Dr. F.F. Jobsis, Durham, North Carolina, U.S.A.
Dr. M. Kessler, Dortmund, Federal Republic of Germany
Dr. A.G.B. Kovach, Budapest, Hungary
Dr. F. Kreuzer, Nijmegen, Netherlands
Dr. LS. Longmuir, Raleigh, North Carolina, U.S.A.
Dr. M. Mochizuki, Yamagata, Japan
Dr. D.O. Reneau, Ruston, Louisiana, U.S.A.
Dr. G. Thews, Mainz, Federal Republic of Germany
Dr. W.J. Whalen, Cleveland, Ohio, U.S.A.
Preface
This volume contains the papers which were presented at the
Third International Symposium on Oxygen Transport to Tissue
together with the discussions at the end of each Session. The
meeting was held at Churchill College, Cambridge from July 4th-7th
1977.
Our special thanks are due to Mrs. Valerie Jeal and Mr. Charles
Drown of the Department of Pathology, Bristol, who were invaluable
in ensuring the smooth running of the meeting and the preparation
of this book. We are very grateful to Dr. Marian Silver for
proof-reading and helping to disentangle the "discussion". We
would also express our thanks for the general help received from
Janet and Fiona Silver and Steven James and our appreciation of
the cheerful assistance of Miss Sadie Williams in putting the
finishing touches to the manuscript.
August, 1977 I.A. Silver
M. Erecinska
H.I. Bicher
Contents
Session 1 - OXYGEN ELECTRODES AND BLOOD
MONITORING DEVICES
The Bitumen P02 Electrode - A New Method to
Manufacture P02 Needle Electrodes 3
H. Acker, D. Sylvester, E. Dufau,
and H. Durst
A Working Equation for Oxygen Sensing
Disk Electrodes • • • • • • 9
T. E. Tang, R. E. Barr, V. G. Murphy,
and A. W. Hahn
Variations on the Response Characteristics
of Oxygen Electrodes •••• 17
R. E. Barr, T. E. Tang, and A. W. Hahn
Directly Heated Transcutaneous Oxygen Sensor 25
H. P. Kimmich, J. G. Spaan, and F. Kreuzer
A Self-Calibrating, Continuous in vivo P02
and PC02 Monitor • • • • • • • •. 31
J. S. Clark, W. D. Wallace, F. L. Farr,
and M. J. Criddle
Thin Ring P02 Electrode Using Gold Paste and
Its Application to the Study of
the Effects of Venous Occlusion
on Skin P02 • • • • • • • • 37
T. Koyama, S. Makinoda, T. Sasajima,
M. Ishikawa, and Y. Kakiuchi
How to Quantify Skeletal Muscle Capillarity 41
J. T. Loats, A. H. Si11au, and N. Banchero
ix
x CONTENTS
Staining of P02 Measuring Points Demonstrated
for the Rat Brain Cortex • • • • 49
H. Metzger, S. Heuber, A. Steinacker,
and J. StrUber
Discussion • • • • 57
Session 2 - OPTICAL METHODS
First Cryophotometric Measurements of the
Hb02 Saturation in the Cat
Carotid Body • • • • • 63
H. Acker, D. W. LUbbers, R. Heinrich,
and W. A. Grunewald
Multiple Wavelength Reflectance Oximetry in
Peripheral Tissues • • • • 69
P. W. Cheung, S. Takatani, and E. A. Ernst
Myoglobin Saturation and Calculated P02 in
Single Cells of Resting Gracilis
Muscles • • • • • • • 77
T. E. J. Gayeski and C. R. Honig
The Myoglobin Probed Optical Studies of
Myocardial Energy Metabolism 85
M. Tamura, N. Oshino, and B. Chance
The Intracellular Heterogeneity of Oxygen
Concentrations as Measured by
Ultraviolet Television
Microscopy of PBA Fluorescence
Quenching by Oxygen • • • • • • 93
I. S. Longmuir. J. A. Knopp, T.-P. Lee,
D. Benson, and A. Tang
Application of the Optode Measurements of
Surface P02 and PC02 of the
Isolated Guinea-Pig Heart • • 99
N. Opitz, H. Weigelt, T. Barankay. and
D. W. LUbbers
A New Method for Measuring the Oxygen Content
in Microliter Samples of Gases
and Liquids: The Oxygen Cuvette 107
R. Zander, W. Lang, and H. U. Wolf
CONTENTS
Factors Influencing the Correction Factor
Used to Eliminate the Apparent
NADH Fluorescence Changes
Caused by Alterations in
Cerebrocortical Blood Content 113
E. D6ra and A. G. B. Kovach
Organ Absorbance and Fluorescence
Spectrophotometry and Its
Application to Oxygen-Dependent
Parameters •••••..••• 119
H. Sies and H. Schwab
An Experimental Model of the Krogh Tissue
Cylinder: Two-Dimensional
Quantitation of the Oxygen Gradient 127
B. Quistorff, B. Chance, and A. Hunding
Discussion • • • 135
Session 3 - MODEL SYSTEMS
Multi-Capillary Model for Oxygen Transport
to Skeletal Tissue •• • • • 139
K. Akmal, D. F. Bruley, N. Banchero,
R. Artigue, and W. Maloney
Dynamic Hypoxic Hypoxemia in Brain Tissue:
Experimental and Theoretical
Methodologies • • 149
W. J. Dorson, Jr. and B. A. Bogue
Mathematical Model of Respiratory Gas
Exchange at Stationary Conditions 157
A. Grad and S. Svetina
Calculation of the Facilitation of O2 or CO
Transport by Hb or Mb by Means of
a New Method for Solving the
Carrier-Diffusion Problem • • • . 163
L. Hoofd and F. Kreuzer
The Effect of Deoxygenation Rate of the
Erythrocyte on Oxygen Transport
to the Cardiac Muscle • • • • • 169
M. Mochizuki and T. Kagawa
CONTENTS
Gas Transfer Through the Skin: A Two-Layer
Model Relating Transcutaneous
Flux to Arterial Tension 175
J. A. Quinn
Electrical Potentials During Carbon Dioxide
Transport in Hemoglobin Solutions 183
J. De Koning, P. Stroeve, and J. H. Meldon
Interpretation of Oxygen Disappearance Rates
in Brain Cortex Following Total
Ischaemia • • • • • • • • 189
D. D. Reneau and J. H. Halsey, Jr.
Analysis of Oxygen Transport in Bullfrogs 199
H. Tazawa and M. Mochizuki
Distribution of Intracapi11ary 02-Supp1y and
Tissue P02 in Canine Left Ventricle
During Normoxia - An Analysis of
Kryophotometrica11y Measured Hb02
Saturation with a Diffusion Model . • • • • •• 205
W. A. Grunewald and W. Sowa
Metabolism Prediction Based on Measurement of
P02 and Flow at the Same Locus
During Seizures and Barbiturate
Suppression of EEG 213
D. D. Reneau, R. S. McFarland, and
J. H. Halsey, Jr.
A Computer Analysis of Graded Ischemia in
Brain. ...••••• 219
R. E. Null and D. D. Reneau
A Comparison of Two Nonclassical Models for
Oxygen Consumption in Brain and
Liver Tissue· •••••• 225
D. G. Buerk and G. M. Saide1
Models for Pulsed Polarographic Electrodes 233
A. C. Pinchak and E. A. Ernst
Mathematical Simulation of Oxygen and Carbon
Dioxide Transport in Fetal Brain 239
J. M. Cameron and D. D. Reneau
Some Effects of High Altitude and Po1ycythaemia
on Oxygen Delivery • • • • • • • • 245
D. D. Reneau and I. A. Silver
