Oxygen Transport in Red Blood Cells. Proceedings of the 12th Aharon Katzir Katchalsky Conference, Tours, France, 4–7 April 1984

209 Pages·1986·4.93 MB·English

by  Claude Nicolau (Eds.)

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OXYGEN TRANSPORT
IN RED BLOOD CELLS
Proceedings of the 12th Aharon Katzir Katchalsky
Conference, Tours, France, 4-7 April 1984
Editor:
CLAUDE NICOLAU
Centre de Biophysique MoJecuJaire,
Orleans, France
PERGAMON PRESS
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First edition 1986
Library of Congress Cataloging in Publication Data
Aharon Karzir-Katchalsky conference (12th: 1984:
Tours, France)
Oxygen transport in red blood cells.
(Advances in the biosciences; v. 54)
Includes index.
1. Oxygen transport (Physiology)—Congresses.
I. Nicolau, Claude. II. Title. III. Series.
[DNLM: 1. Erythrocytes—congresses. 2. Oxygen—blood—
congresses. 3. Oxygen—metabolism—congresses.
W3 Ad244 v. 54/WH 150 A285 19840]
QP99.3.09A38 1984 612'.1111 85-12433
British Library Cataloguing in Publication Data
Aharon Katzir-Katchalsky Conference
{12th : 1984 : Tours)
Oxygen transport in red blood cells : proceedings of the
12th Aharon Katzir-Katchalsky Conference, Tours,
France, April 4—7, 1984. — (Advances in the biosciences;
54)
1. Oxygen transport (Physiology)
I. Title II. Nicolau, Claude III. Series
599'.01'1 QP99.3.09
ISBN 0-08-030800-7
In order to make this volume available as economically and as
rapidly as possible the typescript has been reproduced in its
original form. This method unfortunately has its typographical
limitations but it is hoped that they in no way distract the reader.
Printed in Great Britain by A. Wheaton £r Co. Ltd., Exeter
Foreword
To mark the 12th year since Aharon Katzir-Katchalsky was killed
and continuing a tradition established within the international scientific
community, the Centre National de la Recherche Scientifique together with
the Weizmann Institute of Science, the Aharon Katzir Katchalsky Center or-
ganized in Tours, France, a Meeting on 0-transport by the Red Blood Cells.
2
Many distinguished workers in the field attended this Meeting. Like the
previous ones and like Aharon's laboratory, this Meeting was an encounter
place of biophysicists, biochemists, hematologists, physicians, physiolo-
gists. This volume presents the invited lectures delivered at this Meeting.
A few of them deal with the influence of the Hb-0 dissociation curve on 0
2 2
transport by red blood cells. Other aspects are covered as well and they
bring a significant degree of novelty. Thanks are due to all the authors for
that.
The financial support of the CNRS, the Katzir-Katchalsky Center,
Smith Kline French Laboratories (Philadelphia, PA, USA) and of the Pasteur-
Weizmann Committee is gratefully acknowledged.
Christiane David and Jeannine Florian typed the text and Edith
Barre compiled the Subject Index. My best thanks go to them.
Last but not least Pergamon Press with their usual competence
and efficiency and with unusual patience printed this volume.
Claude NICOLAU
v
ERRATUM
OXYGEN TRANSPORT
IN RED BLOOD CELLS
12th Aharon Katzir-Katchalsky Meeting
Tours, France, 4-7 April 1984
The editor would like to acknowledge the sponsorship of the following:
Centre Regional de Transfusion Sanguine, Tours
Biotest, France
Institut Merieux, France
Travenol, France
Hoechst-Behring, France
Laboratories Pieree Fabre, France
which contributed greatly to the success of the meeting.
List of Contributors
Dr Toshio Asakura Dr B Chaillot
Division of Hematology Faculte des Sciences Pharmaceutiques
The Children's Hospital et Biologiques
of Philadelphia 21033 Dijon Cedex
34th Street and Civic France
Center Boulevard
Philadelphia Dr 0 Charansonney
Pennsylvania 19104 Laboratory of Biophysics
USA Hopital Fernand Widal
200 Rue du Faubourg Saint-Denis
Dr G Avenard 75010 Paris
Centre National de Transfusion France
Sanguine
Paris Dr M Chassaigne
France Centre Regional De Transfusion
Sanguine
Dr P Boivin UA 596 C.N.R.S.
Inserm U 160 2 Boulevard Tonnele
Hopital Beaujon 37044 Itours
92118 Clichy Cedex France
France
Dr C Crone
Dr M A Borchardt Institute of Medical Physiology
Department of Medicine Department A
university of Wisconsin University of Copenhagen
Madison The Panum Institute
Wisconsin 53792 Blegdamsvej 3 c
USA DK-2200 Copenhagen N
Denmark
Dr George H Brewer
Department of Human Genetics Dr E Dellacherie
University of Michigan Ecole Nationale Superieure Des
Ann Arbor Industries Chemiques
MI 48104 CNRS ERA 23
USA 54042 Nancy Cedex
France
IX
X List of Contributors
Dr Robert D Dick Dr J L Martin
Department of Internal Medicine Laboratory of Biophysics
university of Michigan Hopital Fernand Widal
Ann Arbor 200 Rue du Faubourg Saint-Denis
MI 48104 75010 Paris
USA France
Dr M Duruble Dr A Mayevsky
Laboratory of Biophysics Department of Life Sciences
Hopital Fernand Widal Bar-Ilan university
200 Rue du Faubourg Saint-Denis Ramat-Gan 52 100
75010 Paris Israel
France
Dr C Nicolau
Dr M A Duvelleroy Centre de Biophysique Moleculaire
Laboratory of Biophysics 45045 Orleans
Hopital Fernand Widal France
200 Rue du Faubourg Saint-Denis
75010 Paris Dr L Philippe
France Dept of Nuclear Medicine
and ultrasound
Dr Y Gauduel Trousseau University Hospital
Laboratory of Biophysics Tours
Hopital Fernand Widal France
200 Rue du Faubourg Saint-Denis
75010 Paris Dr George K Radda
France Dept of Biochemistry
university of Oxford
Dr Michael P Hlastala South Iferks Road
Depts of Physiology & Biophysics Oxford
University of Washington
Seattle Dr Michael P Reilly
WA 98195 Division of Hematology
USA The Children's Hospital of Philadelphia
34th Street & civic Center Bouleavard
Dr Ernesto E Di lorio Philadelphia
Laboratorium fuer Biochemie I Pennsylvania 19104
Eidgenoessische Technische USA
Hochschule-Eth-Zentrum
Uhiversitaetsstrasse 16 Dr C Ropars
8092 Zurich CNRS ERA 697 and Centre Regional
Switzerland de Transfusion Sanguine
37000 Tours
Er R Itti France
Dept of Nuclear Medicine
and Ultrasound Dr J F Stolz
Trousseau University Hospital Centre Regional de Transfusion
Tours Sanguine et D'Hematologie
France R.N. 74
54500 Vandoeuvre-Les-Nancy
Dr P Labrude France
Centre Regional de Transfusion
Sanguine et D'Hematologie Dr 0 Stucker
R.N. 74 Laboratory of Biophysics
54500 Vandoeuvre-Les-Nancy Hopital Fernand Widal
France 200 Rue du Faubourg Saint-Denis
75010 Paris
France
List of Contributors xi
Dr B Tannenbaum Dr C Vigneron
Dept of life Sciences Centre Regional de Transfusion
Bar-Ilan university Sanguine et D'Hematologie
Ramat-Gan 52 100 R.N. 74
Israel 54500 Vandoeuvre-Les-Nancy
France
Dr B Teisseire
Inserm U 138 & Service Dr Kaspar H Winterhalter
D' Explorat ions Fonctionnelles Laboratorium fuer Biochemie I
Hopital Henri Mondor Eidgenoessische Technische
94010 Creteil Hochschul e-Eth-Zentrum
France Universitaetsstrasse 16
8092 Zurich
Dr R Trouve Switzerland
Laboratory of Biophysics
Hopital Fernand Widal Dr R D Woodson
200 Rue du Faubourg Saint-Denis Department of Medicine
75010 Paris university of Wisconsin
France Madison
Wisconsin 53792
Dr C R Valeri USA
Naval Blood Reserach Lab
Boston University Dr E Yoles
School of Medicine Department of Life Sciences
Boston Bar-Ilan university
MA 02132 Ramat-Gan 52 100
USA Israel
Dr E Vicaut Dr N Zarchin
Laboratory of Biophysics Department of Life Sciences
Hopital Fernand Widal Bar-Ilan tiiiversity
200 Rue du Faubourg Saint-Denis Ramat-Gan 51 100
75010 Paris Israel
France
Dr C Vieilledent
Inserm U 138 & Service
D1Explorations Fonctionelles
Hopital Henri Mondor
94010 Creteil
France
Influence of Heme Pocket Geometry
on Ligand Binding to Heme Proteins
K. H. Winterhalter and E. E. Di Iorio
Laboratorium fuer Biochemie I, Eidgenoessische Technische
Hochschule, ETH-Zentrum, Universitaetsstrasse 16, 8092 Zurich,
Switzerland
Oxygen binding, and its delivery to the place of consumption,
is the continent in which the city of oxygen transport by red
blood cells lies. The problem of oxygen transport is old, but
new insight is gained every year. Table I gives an overview of
the continent and the roads to and from the city. The levels
of traffic regulation, as they are conceived presently, are
also given.
Table I - The levels of regulation of Op supply to tissues
Compartment Level Some parameters
A External 1. p0 Altitude
2
2. Aeration of alveolae State of Lung
3. Permeability of al- State of Lung
veolae
B Blood 1. 0 capacity Erythropoietin levels
2
2. 0 uptake by RBC Potency of circula-
2
tion, membrane per-
meability, diffusion
in cytoplasm, others?
3. 0 affinity of Genetic material, al-
2
hemoglobin losteric effectors,
C0^o2,, ppHH,, others?
4. 0 dissociation in As for B and B-; 0
2 o
tne periphery gradient
C Intracellular 1. Myoglobin State of exercise,
in tissues hormonal regulation
2. Uptake into orga- Diffusion, others?
nelles (Mitochon-
dria, microsomes,
peroxysomes, plas-
mamembranes
1
2 Κ. Η. Winterhalter and Ε. Ε. Di Iorio
Oxygen, at its various places of consumption, is needed for a
variety of different types of metabolism. They are summarized
in table II. From this table it is evident that oxygen affects
all walks of biochemistry in areobic organisms, from energy
production to cancer.
Table II - Types of metabolisms in which oxygen is paramount
TvPe Main organelle Examples
Energy metabo- Mitochondria Respiratory chain;
lism Chloroplasts photosystem 1 and 2
Katabolic Peroxysomes Conversion of octanoate to
H 0 ; D-amino acid break-
2 2
down; urate and L-alpha-
hydroxy-acids metabolism
Modification of Microsomes Synthesis of carcinogens
metabolites and tumor promotors by te-
rminal oxygenase systems;
prostaglandin and adrena-
lin synthesis
Biosynthesis Cytoplasm Some steps of aminoacid
and Katabolism katabolism, e.g. trypto-
phane pyrrolase
Bacterial kil- Plasmamembrane of Polymorphonuclear leuco-
ling white cells; nat- cyte Superoxide synthetase
ural killer cells
As many of the regulatory mechanisms of oxygen transport by
red blood cells (Table I.B) will be discussed by other
participants of this conference, the topic of this talk is
limited to the innermost events inside the hemoglobin
molecule.
The overall anatomy of a sperm whale myoglobin (SW-Mb)
molecule (also representative of a hemoglobin chain) is given
in fig. 1. For the binding to the heme-iron of a ligand
molecule, coming from the solvent, at least three steps,
characterized by energy barriers (Austin, et. al., 1975), must
be distinguished: