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US 20150031070A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2015/0031070 A1 SHOHAM et al. (43) Pub. Date: Jan. 29, 2015 (54) OPTICALLY SENSITIVE CELL NETWORK (60) Provisional application No. 61/582,909, ?led on Jan. 4, 2012. (71) Applicant: TECHNION RESEARCH & DEVELOPMENT FOUNDATION Publication Classi?cation LIMITED, HAIFA (IL) (51) Int. Cl. (72) Inventors: SHY SHOHAM, HAIFA (IL); ANAT G01N 33/50 (200601) MAROM, KIRYAT-BIALIK (IL); (52) us CL SANJEEV KUMAR MAHTO’ HAIFA CPC ................................ .. G01N 33/5058 (2013.01) (IL) USPC ........................................ .. 435/29, 435/2887 (21) Appl. No.: 14/512,557 (57) ABSTRACT (22) Filed: oct' 13’ 2014 A neural network is disclosed. The neural network comprises . . a plurality of optogenetically modi?ed neural cells being Related U's' Apphcatlon Data three-dimensionally distributed in a hydrogel medium and (62) Division of applicationNo. 13/733,163,?1ed on Jan.3, being disconnected from any solid support having a shear 2013, now Pat. No. 8,858,929. modulus above 1 GPa. Patent Application Publication Jan. 29, 2015 Sheet 1 0f 22 US 2015/0031070 A1 PEG. 2 Patent Application Publication Jan. 29, 2015 Sheet 2 0f 22 US 2015/0031070 A1 10x FEG. 3 PEG. 4A 1% FEG. 45 PBS. 46 FEG. 4D Patent Application Publication Jan. 29, 2015 Sheet 3 0f 22 US 2015/0031070 A1 HG. 5 Patent Application Publication Jan. 29, 2015 Sheet 4 0f 22 US 2015/0031070 A1 80 72 i , 5 F58. 68 Patent Application Publication Jan. 29, 2015 Sheet 5 0f 22 US 2015/0031070 A1 F56. 66 PEG. 6D Patent Application Publication Jan. 29, 2015 Sheet 6 0f 22 US 2015/0031070 A1 PEG. 7178 Patent Application Publication Jan. 29, 2015 Sheet 7 0f 22 US 2015/0031070 A1 122 122 “x i 1 32.355 <> ’ i > \ < > 3 lZ—Q 128 ii > < w 12% PEG. 7C PEG. 7D Patent Application Publication Jan. 29, 2015 Sheet 8 0f 22 US 2015/0031070 A1 PEG. 7E FEG. 7F Patent Application Publication Jan. 29, 2015 Sheet 9 0f 22 US 2015/0031070 A1 226 242 202

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LIMITED, HAIFA (IL). (51) Int. Cl. (72) Inventors: SHY SHOHAM, HAIFA (IL); ANAT. G01N 33/50. (200601). MAROM, KIRYAT-BIALIK (IL);. (52) us CL.

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LIMITED, HAIFA (IL). (51) Int. Cl. (72) Inventors: SHY SHOHAM, HAIFA (IL); ANAT. G01N 33/50. (200601). MAROM, KIRYAT-BIALIK (IL);. (52) us CL.

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