Table Of ContentShusheng Zhang · Sai Bi · Xinyue Song
Editors
Nucleic Acid
Amplification Strategies
for Biosensing,
Bioimaging and
Biomedicine
fi
Nucleic Acid Ampli cation Strategies
for Biosensing, Bioimaging and Biomedicine
Shusheng Zhang Sai Bi
(cid:129) (cid:129)
Xinyue Song
Editors
fi
Nucleic Acid Ampli cation
Strategies for Biosensing,
Bioimaging and Biomedicine
123
Editors
ShushengZhang SaiBi
Shandong Provincial KeyLaboratory of Collegeof Chemistry andChemical
Detection Technologyfor Tumour Markers Engineering
Linyi University Qingdao University
Linyi, Shandong,China Qingdao,Shandong,China
Xinyue Song
Shandong Provincial KeyLaboratory of
Detection Technologyfor Tumour Markers,
Collegeof Chemistry andChemical
Engineering
Linyi University
Linyi, Shandong,China
ISBN978-981-13-7043-4 ISBN978-981-13-7044-1 (eBook)
https://doi.org/10.1007/978-981-13-7044-1
LibraryofCongressControlNumber:2019935994
©SpringerNatureSingaporePteLtd.2019
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Nucleic acid amplification strategies are a
new class of strategies including multiple
isothermal and thermocycling amplification
techniques. This book mainly introduces the
typical nucleic acid amplification strategies,
experimental research methods, their
applications andfuturedevelopmenttrendsin
the construction of optical and
electrochemical biosensors, in vitro and
in vivo bioimaging systems and biomedical
platforms. This book can be used as a
reference for scientific researchers in related
disciplines. It can also be used as a teaching
reference book for undergraduate or
postgraduate students in chemistry,
materials, medicine and environment.
Foreword
Nucleic acid amplification strategies have been rapidly developed over the past
years to enable highly sensitive and specific detection of a wide range of biomo-
lecules,anessentialprocessinbiochemicalanalysisandearlyclinicaltestoftumor
markers for cancer diagnosis. Understanding the theory, methodology, character-
isticanddynamicofamplificationstrategiesisofgreatimportancetoin-depthstudy
and application. However, the development of accurate and robust methods based
on nucleic acid amplification is still in urgent need for the analysis of practical
samples.
ProfessorShushengZhang,Dr.SaiBiandDr.XinyueSongkindlyprovidethis
book that focuses on nucleic acid amplification strategies. The overall aim of the
bookistobringtoreadersacompletescopeofnucleicacidamplificationstrategies,
rangingfromfundamentaltheoryandexperimentaltechniquestomajorapplications
and future development trends. Hence, the content of the book covers basic con-
cepts,knowledgeandresearchmethodsofnucleicacidamplificationstrategiesanda
varietyofopticalandelectrochemicaldetectiontechniquesthatarewidelyapplicable
in many fields, especially in biosensing, bioimaging and biomedicine. More
specifically,thisbookisdividedintofourpartswith15chapters.PartI(Chap.1)is
the introduction that gives an overview of the theoretical basis for nucleic acid
amplification strategies, as well as typical examples. Part II (Chaps. 2–10) sum-
marizestheapplicationsofnucleicacidamplificationstrategiesfortheconstruction
of biosensors: The first half, from Chaps. 2 to 6, goes through five major types of
nucleicacidamplificationstrategy-basedopticalbiosensors,includingfluorescence,
chemiluminescence, electrochemiluminescence, colorimetric and surface plasmon
resonance biosensors; and the second half, from Chaps. 7 to 10, discusses
electrochemical-relatedbiosensors,includingelectrochemicalDNAsensors,photo-
electrochemical biosensors, nanopore sensors and quartz crystal microbalance.
Part III (Chaps. 11 and 12) introduces nucleic acid amplification strategy-based
bioimaging, within which Chap. 11 is to summarize nucleic acid amplification
strategy-based fluorescence imaging, including applications of isothermal and
thermal cycling amplification techniques, and Chap. 12 is to describe nucleic acid
amplificationstrategy-basedsurface-enhancedRamanspectroscopyforbioimaging,
vii
viii Foreword
such as nucleic acid-induced distance change and aggregation. Finally, Part IV
(Chaps. 13–15) gives an overview of the current state-of-the-art development
and future perspectives of applying nucleic acid amplification strategies for bio-
medicine:Chap.13focusesonapplications ofnucleicacid amplificationstrategies
forinvitroandinvivodetectionofmetalions;Chap.14highlightsapplicationsof
various nucleic acid amplification strategies in theranostics; and Chap. 15 summa-
rizes and prospects the use of miniaturized devices for nucleic acid amplification
strategies.
Sincenucleicacidamplificationstrategiesareatthestageofdevelopment,many
theoretical models and experimental methods are still being developed and
improved.Relyingonthedeepunderstandingandfruitfulresearchachievementsof
Prof. Shusheng Zhang, the authors present to readers a comprehensive overview
of the emerging nucleic acid amplification strategies and their application and
prospectsinthefieldsofbiosensing,bioimagingandbiomedicine.Itisforsurethat
many readers will be able to learn systematically the theoretical and practical
knowledge, and gain thinking and enlightenment from this book. Readers are also
encouragedtoexploreandcontributetothegrowingliteratureofthisevolvingfield.
We earnestly hope that this book will promote the scientific excitement and con-
tribute to the advance of biosensing, bioimaging and biomedicine.
Nanjing, China Yi-Tao Long, Ph.D.
Professor of Chemistry, School of Chemistry
andChemicalEngineering,NanjingUniversity
Preface
ThewritingandpublishingofNucleicAcidAmplificationStrategiesforBiosensing,
BioimagingandBiomedicineareamatterofcoursewiththevigorousdevelopment
of nucleic acid amplification techniques. This book contains the following two
aspects of significance.
Firstly,nucleicacidamplificationisanemergingtechnologyandcloselyrelated
to life science. Since the invention of polymerase chain reaction (PCR) in 1980s,
PCR-based methods have been widely used in clinical diagnosis and treatment
because trace DNA can be exponentially amplified with high efficiency. In con-
sideration of the complicated temperature control of the thermocycling amplifica-
tiontechnique,isothermalamplificationtechniquesbasedonbothtoolenzymeand
enzymes-free strategy have been developed for signal amplification. With the
specific properties of tool enzymes, such as polymerase, exonuclease and
endonuclease, varioustoolenzyme-basedisothermalamplificationtechniques such
as rolling circle amplification (RCA) and recombinase polymerase amplification
(RPA) have been developed. Based on complementary base pairing, isothermal
amplification techniques such as hybridization chain reaction (HCR) and catalytic
hairpin assembly (CHA) have realized the non-enzymatic amplification with high
efficiency, which further extends the nucleic acid amplification strategies. On the
one hand, the amplification techniques meet the needs of sensitive detection of a
widerangeofbiomolecules.Ontheotherhand,thediverserequirementsofnucleic
acid amplification strategies pose new challenges to the existing methods such as
higher specificity and portability. Novel amplification techniques are needed, and
thedevelopmentdirectionoftheamplificationstrategiesisalsoreflectedinthetopic
of this book.
Secondly, nucleic acid amplification iscloselyintegrated with real applications.
Through combining with optical and electrochemical detection techniques, nucleic
acid amplification strategies have been widely used in biosensing, bioimaging and
biomedicineandbecomemoreandmoreimportantindiseasediagnosis,especially
incancers.Overthepastdecades,thedevelopmentofthenucleicacidamplification
strategies is unprecedented. Under this great situation, how to expand the appli-
cationsofnucleicacidamplificationstrategiesinmoreaspectsshouldbeaddressed.
ix
x Preface
In this book, we cover this topic in the presentation and discussion of the nucleic
acid amplification strategies, such as features, advantages, disadvantages and
challenges.
The compilation and distribution of Nucleic Acid Amplification Strategies for
Biosensing, Bioimaging and Biomedicine also reflect the prosperous development
of biochemical analysis. Each chapter of the book is written by scientists in the
field, which not only summarizes the framework and development of nucleic acid
amplification strategies at a high level, but also provides a systematic introduction
for readers to understand their achievements in biosensing, bioimaging and
biomedicine.
Linyi, China Shusheng Zhang
Qingdao, China Sai Bi
Linyi, China Xinyue Song
Acknowledgements
It is a special privilege to express our heartfelt gratitude and respect for all the
peoplewhohelpeduswiththisbook.Firstandforemost,wewouldliketogiveour
deepestgratitudetotheFundCommittee.Ourworkhasbeenfinanciallysupported
by the National Natural Science Foundation of China and Nature Science
Foundation of Shandong Province. Secondly, we are greatly indebted to all the
professors in our institute who have achieved interesting and meaningful research
work.Lastbutnotleast,wewouldliketoshowourthankstoteachersandstudents
in the Linyi University. With their help and cooperation, we can finish the book
successfully.
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