Table Of ContentNew Techniques
for Studying Biomembranes
Methods in Signal Transduction
Series Editors: Joseph Eichberg Jr., Michael X. Zhu, and Harpreet Singh
The overall theme of this series continues to be the presentation of the wealth of up-to-date research
methods applied to the many facets of signal transduction. Each volume is assembled by one or more
editors who are preeminent in their specialty. In turn, the guiding principle for editors is to recruit chapter
authors who will describe procedures and protocols with which they are intimately familiar in a reader
friendly format. The intent is to assure that each volume will be of maximum practical value to a broad
audience, including students and researchers just entering an area, as well as seasoned investigators.
New Techniques for Studying Biomembranes
Edited by Qiu-Xing Jiang
Polycystic Kidney Disease
Jinghua Hu and Yong Yu
Signal Transduction and Smooth Muscle
Mohamed Trebak and Scott Earley
Autophagy and Signaling
Esther Wong
Lipid-Mediated Signaling Transduction, Second Edition
Eric Murphy, Thad Rosenberger, and Mikhail Golovko
Calcium Entry Channels in Non-Excitable Cells
Juliusz Ashot Kozak and James W. Putney, Jr.
Signaling Mechanisms Regulating T Cell Diversity and Function
Jonathan Soboloff and Dietmar J. Kappes
Gap Junction Channels and Hemichannels
Donglin Bai and Juan C. Saez
TRP Channels
Michael Xi Zhu
Cyclic Nucleotide Signaling
Xiaodong Cheng
Lipid-Mediated Signaling
Eric J. Murphy and Thad A. Rosenberger
Signaling by Toll-Like Receptors
Gregory W. Konat
Signal Transduction in the Retina
Steven J. Fliesler and Oleg G. Kisselev
Analysis of Growth Factor Signaling in Embryos
Malcolm Whitman and Amy K. Sater
Calcium Signaling, Second Edition
James W. Putney, Jr.
G Protein-Coupled Receptors: Structure, Function, and Ligand Screening
Tatsuya Haga and Shigeki Takeda
For more information about this series, please visit: https://www.crcpress.com/Methods-in-Signal-
Transduction-Series/book-series/CRCMETSIGTRA?page=&order=pubdate&size=12&view=list&status=
published, forthcoming
Methods in Signal Transduction
Series Editors: Joseph Eichberg Jr., Michael X. Zhu, and Harpreet Singh
New Techniques
The overall theme of this series continues to be the presentation of the wealth of up-to-date research
methods applied to the many facets of signal transduction. Each volume is assembled by one or more
editors who are preeminent in their specialty. In turn, the guiding principle for editors is to recruit chapter
authors who will describe procedures and protocols with which they are intimately familiar in a reader for Studying Biomembranes
friendly format. The intent is to assure that each volume will be of maximum practical value to a broad
audience, including students and researchers just entering an area, as well as seasoned investigators.
New Techniques for Studying Biomembranes
Edited by Qiu-Xing Jiang
Polycystic Kidney Disease
Jinghua Hu and Yong Yu
Signal Transduction and Smooth Muscle
Mohamed Trebak and Scott Earley
Autophagy and Signaling
Esther Wong
Lipid-Mediated Signaling Transduction, Second Edition
Eric Murphy, Thad Rosenberger, and Mikhail Golovko
Calcium Entry Channels in Non-Excitable Cells
Juliusz Ashot Kozak and James W. Putney, Jr.
Signaling Mechanisms Regulating T Cell Diversity and Function
Jonathan Soboloff and Dietmar J. Kappes
Edited by
Gap Junction Channels and Hemichannels
Donglin Bai and Juan C. Saez Qiu-Xing Jiang
TRP Channels
Michael Xi Zhu
Cyclic Nucleotide Signaling
Xiaodong Cheng
Lipid-Mediated Signaling
Eric J. Murphy and Thad A. Rosenberger
Signaling by Toll-Like Receptors
Gregory W. Konat
Signal Transduction in the Retina
Steven J. Fliesler and Oleg G. Kisselev
Analysis of Growth Factor Signaling in Embryos
Malcolm Whitman and Amy K. Sater
Calcium Signaling, Second Edition
James W. Putney, Jr.
G Protein-Coupled Receptors: Structure, Function, and Ligand Screening
Tatsuya Haga and Shigeki Takeda
For more information about this series, please visit: https://www.crcpress.com/Methods-in-Signal-
Transduction-Series/book-series/CRCMETSIGTRA?page=&order=pubdate&size=12&view=list&status=
published, forthcoming
First edition published 2020
by CRC Press
6000 Broken Sound Parkway NW, Suite 300, Boca Raton, FL 33487-2742
and by CRC Press
2 Park Square, Milton Park, Abingdon, Oxon, OX14 4RN
© 2020 Taylor & Francis Group, LLC
CRC Press is an imprint of Taylor & Francis Group, LLC
Reasonable efforts have been made to publish reliable data and information, but the author and publisher
cannot assume responsibility for the validity of all materials or the consequences of their use. The authors
and publishers have attempted to trace the copyright holders of all material reproduced in this publica-
tion and apologize to copyright holders if permission to publish in this form has not been obtained. If
any copyright material has not been acknowledged please write and let us know so we may rectify in any
future reprint.
Except as permitted under U.S. Copyright Law, no part of this book may be reprinted, reproduced,
transmitted, or utilized in any form by any electronic, mechanical, or other means, now known or hereafter
invented, including photocopying, microfilming, and recording, or in any information storage or retrieval
system, without written permission from the publishers.
For permission to photocopy or use material electronically from this work, access www.copyright.com or
contact the Copyright Clearance Center, Inc. (CCC), 222 Rosewood Drive, Danvers, MA 01923, 978-750-
8400. For works that are not available on CCC please contact [email protected]
Trademark notice: Product or corporate names may be trademarks or registered trademarks, and are used
only for identification and explanation without intent to infringe.
ISBN: 9781138618060 (hbk)
ISBN: 9780429461385 (ebk)
Typeset in Times
by Lumina Datamatics Limited
Contents
Preface......................................................................................................................vii
Editor ........................................................................................................................ix
Contributors ..............................................................................................................xi
Chapter 1 Introduction: New Tools for Challenges in Membrane Biology ..........1
Qiu-Xing Jiang
Chapter 2 Lipidomics in Human Cancer and Malnutrition ..................................9
Iqbal Mahmud and Timothy J. Garrett
Chapter 3 Tracking and Imaging: SERS Characterization
of Biomembranes ................................................................................25
Wen Ren and Joseph Irudayaraj
First edition published 2020 Chapter 4 Scanning Angle Interference Microscopy (SAIM):
by CRC Press Theory, Acquisition, Analysis and Biological Applications ..............39
6000 Broken Sound Parkway NW, Suite 300, Boca Raton, FL 33487-2742
Cristina Bertocchi, Timothy J. Rudge, and Andrea Ravasio
and by CRC Press
2 Park Square, Milton Park, Abingdon, Oxon, OX14 4RN
Chapter 5 Atomic Force Microscopy of Biomembranes: A Tool
© 2020 Taylor & Francis Group, LLC
for Studying the Dynamic Behavior of Membrane Proteins ..............63
CRC Press is an imprint of Taylor & Francis Group, LLC
Yi Ruan, Lorena Redondo-Morata, and Simon Scheuring
Reasonable efforts have been made to publish reliable data and information, but the author and publisher
cannot assume responsibility for the validity of all materials or the consequences of their use. The authors
and publishers have attempted to trace the copyright holders of all material reproduced in this publica- Chapter 6 Reconstituted Membrane Systems for Assaying
tion and apologize to copyright holders if permission to publish in this form has not been obtained. If
Membrane Proteins in Controlled Lipid Environments .....................93
any copyright material has not been acknowledged please write and let us know so we may rectify in any
future reprint.
Gaya P. Yadav and Qiu-Xing Jiang
Except as permitted under U.S. Copyright Law, no part of this book may be reprinted, reproduced,
transmitted, or utilized in any form by any electronic, mechanical, or other means, now known or hereafter
invented, including photocopying, microfilming, and recording, or in any information storage or retrieval Chapter 7 Cholesterol Modulation of BK (MaxiK; Slo1) Channels:
system, without written permission from the publishers. Mechanistic and Pharmacological Aspects ......................................123
For permission to photocopy or use material electronically from this work, access www.copyright.com or Alex M. Dopico, Anna N. Bukiya, and Kelsey North
contact the Copyright Clearance Center, Inc. (CCC), 222 Rosewood Drive, Danvers, MA 01923, 978-750-
8400. For works that are not available on CCC please contact [email protected]
Chapter 8 Restoration of Membrane Environments for Membrane
Trademark notice: Product or corporate names may be trademarks or registered trademarks, and are used
only for identification and explanation without intent to infringe. Proteins for Structural and Functional Studies ................................145
ISBN: 9781138618060 (hbk) Liguo Wang
ISBN: 9780429461385 (ebk)
Typeset in Times Index ......................................................................................................................161
by Lumina Datamatics Limited
v
Preface
While working on the structural basis and the energetics of the lipid-dependent
gating of voltage-gated potassium channels, a new hypothesis which was raised
by my laboratory and has stood the test of time, I realized that the available tech-
niques for studying lipids and membrane proteins in artificial and cell membranes
have various limitations. My laboratory has implemented various membrane sys-
tems and imaging techniques and developed our own bead-supported unilamellar
membrane (bSUM) system. New techniques have been developed by others in dif-
ferent directions in the past decades and could be systematically introduced in new
books to researchers in the field and interested readers. Upon the suggestions of
Dr. Michael X. Zhu at the University of Texas Health Science Center at Houston,
Texas, USA, I took the task of editing this book and a forthcoming book on biologi-
cal membranes (biomembranes). New Techniques for Studying Biomembranes will
introduce the latest technologies and the forthcoming book, Lipid Determanant
of Membrane-Protein Interactions will be focused on the lipid effects on specific
membrane proteins. This book is the result of a year or so of preparative work. My
initial goal in this part is to introduce newer techniques for analysis of biomem-
branes. New technologies always drive our studies to a higher level and a newer
frontier. Lack of high sensitivity and high spatial and temporal resolutions in study-
ing individual lipid molecules in biomembranes has been a long-standing bottle-
neck. Different techniques to be introduced in the main body of the book reflect the
ample efforts of different investigators in this area. I hope that some of these efforts
will ultimately lead to tools for studying individual lipid and protein molecules in
a native environment, which once achieved will reveal unprecedented insights into
lipid-protein interactions and the co-evolution of membrane proteins with the lipid
environments of their native niches.
I would like to take this opportunity to thank Dr. Zhu and all contributing authors.
The editorial team at CRC Press, including Dr. Chuck R. Crumly, Mrs. Ana Lucia
Eberhart, and their colleagues worked hard to accelerate the publication of this work.
I am deeply indebted to their time and efforts. This work is dedicated to Dr. David
Gadsby of the Rockefeller University, who passed away early this year and had been
a giant in structure–function studies of membrane proteins and whose work has
influenced my own work significantly in the past.
Qiu-Xing Jiang
Gainesville, Florida
vii
Editor
Dr. Qiu-Xing Jiang is an associate professor in the Department of Microbiology
and Cell Science at the University of Florida, and the head of the Laboratory of
Cell Physiology and Molecular Biophysics. In 2020, he is becoming the Director
of cryoEM Center at the Hauptman-Woodward Medical Research Institute in
Buffalo, New York. He earned a BS in biophysics from University of Science and
Technology of China, an MS in cellular biophysics from the Institute of Biophysics
at the Chinese Academy of Sciences, and a PhD in cellular and molecular physi-
ology at Yale University. He did a postdoc training at The Rockefeller University
before starting his independent research. Afterward, he was an assistant profes-
sor of cell biology at UT Southwestern Medical Center in Dallas until he relocated
his lab to Florida in 2015. Dr. Jiang received a EUREKA award from NIH of the
United States in 2009 and a National Innovative Award from AHA in 2012. His lab
has performed substantial amounts of work on membrane biology and molecular
biophysics. Currently Dr. Jiang’s research programs focus on three general areas:
(1) membrane biology with main interests in lipid-dependent gating of voltage-gated
ion channels and roles of membrane proteins in the regulated secretory pathway;
(2) intracellular RNA-binding complexes and the structural basis for their signaling;
and (3) chemical functionalization at the nanometer scale and its application to both
cryoEM imaging and biological questions.
ix
