Table Of ContentAdvances in Experimental Medicine and Biology 1239
Lynne M. Coluccio Editor
Myosins
A Superfamily of Molecular Motors
Second Edition
Advances in Experimental Medicine
and Biology
Volume 1239
Series Editors
Wim E. Crusio, Institut de Neurosciences Cognitives et Intégratives
d’Aquitaine, CNRS and University of Bordeaux UMR 5287, Pessac Cedex,
France
John D. Lambris, University of Pennsylvania, Philadelphia, PA, USA
Heinfried H. Radeke, Institute of Pharmacology & Toxicology, Clinic of the
Goethe University Frankfurt Main, Frankfurt am Main, Hessen, Germany
Nima Rezaei, Research Center for Immunodeficiencies, Children’s Medical
Center, Tehran University of Medical Sciences, Tehran, Iran
Advances in Experimental Medicine and Biology provides a platform for
scientific contributions in the main disciplines of the biomedicine and the life
sciences. This series publishes thematic volumes on contemporary research
in the areas of microbiology, immunology, neurosciences, biochemistry,
biomedical engineering, genetics, physiology, and cancer research. Covering
emerging topics and techniques in basic and clinical science, it brings together
clinicians and researchers from various fields.
Advances in Experimental Medicine and Biology has been publishing
exceptional works in the field for over 40 years, and is indexed in SCOPUS,
Medline (PubMed), Journal Citation Reports/Science Edition, Science
Citation Index Expanded (SciSearch, Web of Science), EMBASE, BIOSIS,
Reaxys, EMBiology, the Chemical Abstracts Service (CAS), and Pathway
Studio.
2018 Impact Factor: 2.126.
More information about this series at http://www.springer.com/series/5584
Lynne M. Coluccio
Editor
Myosins
A Superfamily of Molecular Motors
Second Edition
Editor
Lynne M. Coluccio
Department of Physiology & Biophysics
Boston University School of Medicine
Boston, MA, USA
ISSN 0065-2598 ISSN 2214-8019 (electronic)
Advances in Experimental Medicine and Biology
ISBN 978-3-030-38061-8 ISBN 978-3-030-38062-5 (eBook)
https://doi.org/10.1007/978-3-030-38062-5
© Springer Nature Switzerland AG 2008, 2020
This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or
part of the material is concerned, specifically the rights of translation, reprinting, reuse of
illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way,
and transmission or information storage and retrieval, electronic adaptation, computer software,
or by similar or dissimilar methodology now known or hereafter developed.
The use of general descriptive names, registered names, trademarks, service marks, etc. in this
publication does not imply, even in the absence of a specific statement, that such names are
exempt from the relevant protective laws and regulations and therefore free for general use.
The publisher, the authors, and the editors are safe to assume that the advice and information in
this book are believed to be true and accurate at the date of publication. Neither the publisher nor
the authors or the editors give a warranty, expressed or implied, with respect to the material
contained herein or for any errors or omissions that may have been made. The publisher remains
neutral with regard to jurisdictional claims in published maps and institutional affiliations.
This Springer imprint is published by the registered company Springer Nature Switzerland AG.
The registered company address is: Gewerbestrasse 11, 6330 Cham, Switzerland
Preface
In the years since publication in 2008 of Myosins: A Superfamily of Molecular
Motors, considerable new strides have been made in understanding the struc-
ture and function of myosin motors. In particular, the application of advanced
cell imaging approaches, the development of new animal models, the avail-
ability of new crystal structures, and the discovery of isoform-specific inhibi-
tors have propelled the field forward. This volume addresses the current state
of our collective knowledge regarding myosin structure and function. The
chapters are written by internationally recognized experts in molecular
motors. It is a testament to the importance of this endeavor that authors of
chapters in the 2008 volume who were asked to contribute to this new volume
all enthusiastically agreed. In addition, new authors were effortlessly recruited
to cover new areas including the more recently discovered myosins XVI,
XVIII, and XIX, myosin inhibitors, and the structural basis for cargo binding.
The hope is that this volume will serve as a reference for both investigators
and students and stimulate new research on this amazing family of motor
molecules. I am sincerely grateful to the contributors who made this volume
possible. It has been my privilege and pleasure to work with each of them. I
would also like to thank Gonzalo Cordova from Springer Nature BV,
Dordrecht, for the opportunity to edit this book. The careful editing by
Springer staff members is greatly appreciated.
Boston, MA, USA Lynne M. Coluccio
October 2019
v
Contents
1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Lynne M. Coluccio
2 Myosin Structures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
H. Lee Sweeney, Anne Houdusse, and Julien Robert-Paganin
3 Cargo Binding by Unconventional Myosins . . . . . . . . . . . . . . . . 21
Jianchao Li and Mingjie Zhang
4 The Structure of Acto-Myosin . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Rasmus R. Schröder
5 Small Molecule Effectors of Myosin Function . . . . . . . . . . . . . . 61
Dietmar J. Manstein and Matthias Preller
6 Single-Molecule Biophysical Techniques
to Study Actomyosin Force Transduction . . . . . . . . . . . . . . . . . . 85
Yasuharu Takagi, Nikolas Hundt, and Adam Fineberg
7 High-Speed Atomic Force Microscopy
to Study Myosin Motility . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 127
Noriyuki Kodera and Toshio Ando
8 How Myosin 5 Walks Deduced
from Single-Molecule Biophysical Approaches . . . . . . . . . . . . . 153
James R. Sellers and Yasuharu Takagi
9 How Actin Tracks Affect Myosin Motors . . . . . . . . . . . . . . . . . . 183
Alicja Santos, Yauhen Shauchuk, Urszula Cichoń,
Kevin C. Vavra, and Ronald S. Rock
10 Myosins in the Nucleus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 199
Ivan V. Maly and Wilma A. Hofmann
11 Myosins in Cytokinesis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 233
Thomas D. Pollard
12 Myosins and Disease . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 245
Lynne M. Coluccio
13 Myosins and Hearing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 317
Thomas B. Friedman, Inna A. Belyantseva,
and Gregory I. Frolenkov
vii
viii Contents
14 The Actomyosin Systems in Apicomplexa . . . . . . . . . . . . . . . . . . 331
Karine Frénal, Aarti Krishnan, and Dominique Soldati-Favre
15 Approaches to Identify and Characterise
MYO6-Cargo Interactions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 355
Thomas O’Loughlin, John Kendrick-Jones, and Folma Buss
16 Class IX Myosins: Motorized RhoGAP
Signaling Molecules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 381
Peter J. Hanley, Veith Vollmer, and Martin Bähler
17 Myosin X . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 391
Hiroshi Tokuo
18 Myosin XVI . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 405
Beáta Bugyi and András Kengyel
19 Myosin XVIII. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 421
Manuel H. Taft and Sharissa L. Latham
20 Myosin XIX . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 439
Jennifer L. Bocanegra, Rebecca Adikes,
and Omar A. Quintero
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 453
Contributors
Rebecca Adikes Department of Biochemistry and Cell Biology, 401 Life
Sciences Building, Stony Brook University, Stony Brook, NY, USA
Toshio Ando Nano Life Science Institute (WPI NanoLSI), Kanazawa
University, Kanazawa, Japan
Martin Bähler Institute for Molecular Cell Biology, Westfälische Wilhelms-
Universität Münster, Münster, Germany
Inna A. Belyantseva National Institute on Deafness and Other
Communication Disorders, National Institutes of Health, Section on Human
Genetics, Bethesda, MD, USA
Jennifer L. Bocanegra Department of Biochemistry, University of
Washington, Seattle, WA, USA
Beáta Bugyi Department of Biophysics, Medical School, University of
Pecs, Pecs, Hungary
Folma Buss Cambridge Institute for Medical Research, University of
Cambridge, The Keith Peters Building, Cambridge, UK
Urszula Cichoń Department of Biochemistry and Molecular Biology,
The University of Chicago, Chicago, IL, USA
Lynne M. Coluccio Department of Physiology & Biophysics, Boston
University School of Medicine, Boston, MA, USA
Adam Fineberg Physical and Theoretical Chemistry Laboratory (PTCL),
Department of Chemistry, University of Oxford, Oxford, UK
Karine Frénal Microbiologie Fondamentale et Pathogénicité, UMR 5234,
University of Bordeaux and CNRS, Bordeaux Cedex, France
Thomas B. Friedman Laboratory of Molecular Genetics, National Institute
on Deafness and Other Communication Disorders, Porter Neuroscience
Research Center, National Institutes of Health, Bethesda, MD, USA
Gregory I. Frolenkov Department of Physiology, University of Kentucky
College of Medicine, Lexington, KY, USA
Peter J. Hanley Institute of Molecular Cell Biology, Westfalian Wilhelms
University Münster, Münster, Germany
ix
x Contributors
Wilma A. Hofmann Department of Physiology and Biophysics, Jacobs
School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo,
NY, USA
Anne Houdusse Institut Curie, CNRS, Paris Cedex 05, France
Nikolas Hundt Department of Cellular Physiology, Ludwig-Maximilians-
Universität München, Munich, Germany
John Kendrick-Jones MRC Laboratory of Molecular Biology, Cambridge,
UK
András Kengyel Department of Biophysics, Medical School, University of
Pecs, Pecs, Hungary
Noriyuki Kodera Nano Life Science Institute (WPI NanoLSI), Kanazawa
University, Kanazawa, Japan
Aarti Krishnan Department of Microbiology and Molecular Medicine,
Faculty of Medicine, University of Geneva, Geneva, Switzerland
Sharissa L. Latham The Kinghorn Cancer Centre, Garvan Institute of
Medical Research, Darlinghurst, NSW, Australia
St Vincent’s Clinical School, Faculty of Medicine, UNSW Sydney,
Darlinghurst, NSW, Australia
Jianchao Li Division of Cell, Developmental and Integrative Biology,
School of Medicine, South China University of Technology, Guangzhou,
China
Ivan V. Maly Department of Physiology and Biophysics, Jacobs School of
Medicine and Biomedical Sciences, State University of New York, Buffalo,
NY, USA
Dietmar J. Manstein Hannover Medical School, Institute for Biophysical
Chemistry, Fritz-Hartmann Center for Molecular Medicine, Hannover,
Germany
Thomas O’Loughlin Cambridge Institute for Medical Research, University
of Cambridge, The Keith Peters Building, Cambridge, UK
Thomas D. Pollard Department of Molecular, Cellular and Developmental
Biology, Department of Molecular Biophysics and Biochemistry, Department
of Cell Biology, Yale University, New Haven, CT, USA
Matthias Preller Hannover Medical School, Institute for Biophysical
Chemistry, Hannover, Germany
German Electron Synchrotron (DESY), Hamburg, Germany
Omar A. Quintero Department of Biology, University of Richmond,
Richmond, VA, USA
Julien Robert-Paganin Institut Curie, CNRS, Paris Cedex 05, France
Ronald S. Rock Department of Biochemistry and Molecular Biology, The
University of Chicago, Chicago, IL, USA
