Table Of ContentMolecular Mechanisms of Cellular Growth
Developments in Molecular and Cellular Biochemistry
Series Editor: Naranjan S. Dhalla, Ph.D., FACC
1. Victor A. Najjar (ed.): Biological Effects of Glutamic Acid and Its Derivatives. 1981 ISBN 90-6193-841-4
2. Victor A. Najjar (ed.): Immunologically Active Peptides. 1981 ISBN 90-6193-842-2
3. Victor A. Najjar (ed.): Enzyme Induction and Modulation. 1983 ISBN 0-89838-583-0
4. Victor A. Najjar and Laszlo Lorand (eds.): Transglutaminase. 1984 ISBN 0-89838-593-8
5. Ger J. van der Vusse (ed.): Lipid Metabolism in Normoxic and Ischemic Heart. 1989 ISBN 0-7923-0479-9
6. Jan F.C. Glatz & Ger J. van der Vusse (eds.): Cellular Fatty Acid-Binding Proteins. 1990
ISBN 0-7923-0896-4
7. H.E. Morgan (ed.): Molecular Mechanisms of Cellular Growth. 1991 ISBN 0-7923-1183-3
Molecular Mechanisms
of Cellular Growth
edited by
HOWARD E. MORGAN
Senior Vice President
Geisinger Clinic
Weis Center for Research
Danville, Pennsylvania 178222-2601, USA
Reprinted from Molecular and Cellular Biochemistry, Volume 104, Nos. 1 & 2 (1991)
Springer- Science+Business Media, B.V.
Library of Congress Cataloging-in Publication Data
Molecular mechanisins of cellular growth / edited by Howard E. Morgan
... [et al. ].
p. cm. — (Developments in molecular and cellular
biochemistry v. 7)
;
"Reprinted from Molecular and cellular biochemistry, volume 104."
ISBN 978-1-4613-6733-8 ISBN 978-1-4615-3886-8 (eBook)
DOI 10.1007/978-1-4615-3886-8
1. Cells—Growth—Regulation—Congresses. 2. Paracrine
mechanisms—Congresses. 3. Autocrine mechanisms—Congresses.
I. Morgan, H. E. (Howard E.), 1927- . II. Molecular and cellular
biochemistry. III. Series.
QH604.M66 1991
574.87" 61—dc20 91-8478
CIP
Copyright
© 1991 by Springer Science+Business Media Dordrecht
Originally published by Kluwer Academic Publishers in 1991
Softcover reprint of the hardcover 1st edition 1991
All Rights Reserved. No part of this publication may be reproduced, stored in a
retrieval system, or transmitted in any form or by any means, mechanical, photocopy
ing, recording, or otherwise, without the prior written permission of the publishers,
Springer-Science+Business Media, B.V..
CONTENTS
MOLECULARMECHANISMSOFCELLULARGROWTHSYMPOSIUM
ASCIENTIFIC SYMPOSIUMWAS HELD ATGEISINGER ONJUNE 17-21, 1990TO CELEBRATE
THE75THANNIVERSARYOFTHEGEISINGERHEALTHCARESYSTEM
Foreword
H.-E.Morgan:GeisingerClinic- 75yearsofcommitmenttopatientcare,educationandresearch
SIGNALGENERATION
E. N. Olson,T.1. Brennan,T. Chakraborty,T.-C. Cheng,P. Cserjesi, D. Edmondson,G. James andL. Li:
Molecularcontrolofmyogenesis:antagonismbetweengrowthanddifferentiation 7
T.Jackson,M. F.Allard,C.M.Sreenan,L.K. Doss,S.P.BishopandJ.L.Swain:Transgenicanimalsasa
toolforstudyingtheeffectofthec-mycproto-oncogeneoncardiacdevelopment 15
D.J.Carey:Biologicalfunctionsofproteoglycans:useofspecificinhibitorsofproteoglycansynthesis 21
Y. Liu and D. R. Storm: Expression ofa neuromodulin-B-galactosidase fusion protein in primary cultured
neuronsanditsaccumulationingrowthcones 29
P. C. Simpson,K. Kariya,L. R. Karns, C. S.Longand1.S. Karliner: Adrenergic hormonesandcontrolof
cardiacmyocytegrowth 35
T.C.Peeler,K. M. Baker,C.F.EsmurdocandM. I.Chemin:Angiotensinconvertingenzymeinhibitionin
Dahlsalt-sensitiverats 45
P. A. Watson, K. E.GigerandC.M. Frankenfield: ActivationofadenylatecyclaseduringswellingofS49
cellsinhypotonicmediumisnotinvolvedinsubsequentvolumeregulation 51
F.Sachs:Mechanicaltransductionbymembraneionchannels:aminireview 57
SIGNALTRANSDUCTION
K. A. Foster, P.1. McDermottand J. D. Robishaw: Theeffectofculture and membrane potential on Goa
expressioninneonatalratcardiacmyocytes 63
J. Krupinski:Theadenylylcyclasefamily 73
1.H.Exton,S.J.Taylor,G.AugertandS.B.Bocckino:Cellsignallingthroughphospholipidbreakdown 81
MODULATIONOFGENEEXPRESSIONBYINTRACELLULARSIGNALS
1.LiuandR.W. Hanson:Regulationofphosphoenolpyruvatecarboxykinase(GTP)genetranscription 89
K. R. Boheler, L. Carrier, C. Chassagne, D. de laBastie,J. J. Mercadierand K. Schwartz: Regulation of
myosinheavychainandactinisogenesexpressionduringcardiacgrowth 101
1. H. Reese and W. A. Maltese: Post-translational modification of proteins by 15-carbon and 20-carbon
isoprenoidsinthreemammaliancelllines 109
RIBOSOMEBIOGENESIS:TRANSCRIPTIONOFrDNA
M. R. Paule,E. Bateman,L. Hoffman,C. Iida,M. Imboden,W. Kubaska, P. Kownin, H. Li, A. Lofquist,
P.Risi,Q.YangandM.Zwick:InitiationandregulationmechanismsofribosomalRNAtranscriptionin
theeukaryoteAcanthamoebacastellanii 119
W.Q.Xie,D.1.O'Mahony,S.D. SmithandL.Rothblum:Complementaryinvivoandinvitroanalysesof
theinteractionsbetweenthecis-actingelementsoftheratrDNApromoter 127
A.Schnapp,H.RosenbauerandI.Grummt:Trans-actingfactorsinvolvedinspecies-specificityandcontrol
ofmouseribosomalgenetranscription 137
B. Sollner-Webb,L.Pape,K.Ryan,E. B.Mougey,R. Porretta,E. Nikolov,M. H. Paalman,I.Lazdinsand
C.Martin:ExpressionofmouseandfrogrDNAgenes:transcriptionandprocessing 149
S. T. Jacob, J. Zhang, L. C. Garg and C.-B. Book: Multiple functional enhancer motifs ofrat ribosomal
pM l~
S. P. Johnson and J. R. Warner: Termination of transcription of ribosomal RNA in Saccharomyces
cerevisiae 163
T. Haneda and P. J. McDermott: Stimulation ofribosomal RNA synthesis during hypertrophic growth of
culturedheartcellsbyphorbolester 169
RIBOSOME BIOGENESIS SYNTHESIS OF RIBOSOMAL PROTEINS AND RIBOSOME
FORMATION
W. H. Magerand R. J. Planta: Coordinateexpressionofribosomal protein genes in yeastas a function of
cellulargrowthrate 181
P. Zahradka, D. E. Larson and B. H. Sells: Regulation of ribosome biogenesis in differentiated rat
myotubes 189
P.B.MahajanandE.A.Thompson:GlucocorticoidregulationofrRNAsynthesis 195
H. W. Weber, S. Vallett, L. Neilson, M. Grotke, Y. Chao, M. Brudnak, A. S. Juan and M. Pellegrini:
Serum, insulin and phorbol esters stimulate rRNA and tRNA gene expression in both dividing and
nondividingDrosophilacells 201
Index 209
MolecularandCellularBiochemistry104:1-3,1991.
©1991KluwerAcademicPublishers.
Foreword: Geisinger Clinic -75 years ofcommitment to patient care,
education and research
In1990theGeisingerHealthCareSystemcelebratedits75thanniversary. Sinceitsfounding, Geisingerhas
grownfrom asingle63-bedhospitalinDanvilleintoamulti-institutionalsystemofhealthcarethatdelivers
servicesin45 locationsthroughout central and northeastern Pennsylvania.
Thesystem includes:
- GeisingerFoundation- parentorganization.
- GeisingerClinic- themulti-specialty,salariedphysiciangroup,whichincludesmorethan460physicians
practicing in 65 specialty areas. The clinic administers the research program and conducts continuing
medicaleducationprograms. GeisingerClinicphysiciansrecorded more than1millionpatientvisitslast
year.
- GeisingerMedical Center- a577-bed regionaltertiary referral centerlocatedinDanville, PA.
- Geisinger Wyoming ValleyMedical Center- a230-bedsecondaryreferral centerin Wilkes-Barre.
- Marworth- anationally-recognizedalcoholandchemicaldependencytreatmentcenterthatoperatestwo
facilities- onein Waverly, nearScranton, andone in ShawneeonDelaware, near the DelawareWater
Gap.
- GeisingerHealthPlan- a88,000memberhealthmaintenanceorganizationlicensedtodeliverservicesin
17Pennsylvaniacounties.
- Geisinger Medical ManagementCorporation- afor-profit corporation that providescontract manage
ment, consulting, and other medical servicesto health care providers inside and outside the Geisinger
system.
TheSigfriedandJanetWeisCenterforResearchstandsastangibleevidenceoftheGeisingerorganization's
commitment to fulfilling its triple missionofpatientcare, education, and research.
Dedicated in September 1987, the center includes 65,000 square feet oflaboratories, conference areas,
offices, and informal meeting rooms. Initially opened with a shelled-in third floor, the center has ten
laboratoriesfor itstenstaffscientists, eightassociatescientists,seven postdoctoralfellows, and25research
technicians.
Focusingatthecellularandmolecularlevelofcardiovascularfunction,workinprogressisfundedthisyear
at more than $1.3 million by outside agencies, including the National Institutes ofHealth, the American
Heart Association, Mars Foundation, NationalScienceFoundation, and theArthritis Foundation.
Currentresearchtopicsincludestudiesofextracellularmatrixproteinsandcontrolofcellulargrowth,the
role ofrenin angiotensinsystemsincardiaccellgrowth,secondmessengersinphysicalsignaltransduction,
the expressionofG-proteins in myocytes, post-translationalmodificationofproteinsby isoprenoids.
Other topics include the control of cell cycling, transacting factors in rDNA expressions, effects of
contractionon extracellularmatrix protein genes, and control ofcontraction ofvascularsmoothmuscles.
TheSigfriedandJanetWeisCenterforResearchhasbeen namedto honorthecommitmentoftheWeis
familytotheGeisingersystem.ThesupportofSigfriedandJanetWeishasbeenkeyininitiationoftheBasic
Research Program at Geisinger and in its continued development. To recognize these contributions, the
publicationofthisscientificsymposiumentitled'MolecularMechanismsofCellularGrowth'isdedicatedto
Sigfried and Janet Weis. The symposium was held at Geisingeron June 17-21, 1990to celebrate the 75th
Anniversaryofthe Geisinger HealthCare System.
2
SigfriedandJanetWeis
Sigfried Weis became chairman ofthe Geisinger Medical CenterBoard ofDirectors in 1978. Since 1981,
whenhebecamethefirst chairmanoftheGeisingerFoundation,Mr. Weishasbeenasourceofinspiration
and wisdom for Geisinger's transformation into a multi-institutional systemofhealthcare. The Geisinger
systemtoday isone ofthe largestnot-for-profit healthsystemsintheeastern UnitedStates.
Janet Weis, a published author and civic leader, has served for five years as co-chairperson of the
Children'sMiracleNetworkTelethonatGeisingerMedicalCenter. Shehascontributedherenergytohelp
the GeisingerTelethonraise more than$3.8million to 'helpmake miracleshappen'.
TheWeis family has along history ofactive communityinvolvement. The Sigmundand Claire G. Weis
CenterforthePerformingArtsatBucknellUniversityisnamedinhonorofMr. Weis'sparents.Brother-in-
3
lawCharlesDegensteinhasestablishedtheDegensteinFundforDistinguishedScientistsintheWeisCenter
for Research at Geisinger. The lobbyofthe research centerhasbeen namedin hishonor.
On behalfofthe GeisingerHealthCare System, Iwant to thankthe participantsoftheSymposiumand
thosewhosubmittedpapersforthisfocussed issueofMolecularandCellularBiochemistry. Iamespecially
appreciative for the supportofNaranjan S. Dhalla, Editorwho invited publication ofthese papers.
H.E. Morgan, M.D., Guest Editor
SeniorVice Presidentfor Research
GeisingerClinic, Sigfriedand Janet WeisCenter, for Research,
Danville, PA17822-2601,USA
SIGNAL GENERATION
