MOLECULAR MAPPING OF THE GENOMIC REGIONS ASSOCIATED WITH ALKALINITY ...

100 Pages·2016·0.91 MB·English

MOLECULAR MAPPING OF THE GENOMIC REGIONS
ASSOCIATED WITH ALKALINITY TOLERANCE IN
RICE (Oryza sativa L.)

By

TALARI JYOTHSNA
B.Sc. (Ag.)

THESIS SUBMITTED TO
ACHARYA N. G. RANGA AGRICULTURAL UNIVERSITY
IN PARTIAL FULFILMENT OF THE REQUIREMENTS
FOR THE AWARD OF THE DEGREE OF

MASTER OF SCIENCE IN AGRICULTURE

DEPARTMENT OF GENETICS AND PLANT BREEDING
COLLEGE OF AGRICULTURE
ACHARYA N. G. RANGA AGRICULTURAL UNIVERSITY
RAJENDRANAGAR, HYDERABAD - 500 030.

JULY 2009
CERTIFICATE

This is to certify that Miss. TALARI JYOTHSNA has satisfactorily prosecuted
the course of research and that the thesis entitled “MOLECULAR MAPPING OF
THE GENOMIC REGIONS ASSOCIATED WITH ALKALINITY TOLERANCE
IN RICE (Oryza sativa L.)” submitted is the result of original research work and is of
sufficiently high standard to warrant its presentation to the examination. I also certify
that the thesis or part thereof has not been previously submitted by her for a degree of
any university.

Place: Hyderabad (K. RADHIKA)
Date : Chairman of the Advisory Committee
CERTIFICATE

This is to certify that the thesis entitled “MOLECULAR MAPPING OF THE
GENOMIC REGIONS ASSOCIATED WITH ALKALINITY TOLERANCE IN
RICE (Oryza sativa L.)” submitted in partial fulfillment of the requirements for the
degree of MASTER OF SCIENCE IN AGRICULTURE (GENETICS AND
PLANT BREEDING) of Acharya N. G. Ranga Agricultural University, Hyderabad, is
the record of the bonafide research work carried out by Miss. TALARI JYOTHSNA
under our guidance and supervision. The Student Advisory Committee has approved the
subject of the thesis.
No part of the thesis has been submitted by the student for any other degree or
diploma or has been published. The published part has been fully acknowledged. All the
assistance and help received during the course of investigation have been duly
acknowledged by the author of the thesis.
(K. RADHIKA)
Chairman of the Advisory Committee

Thesis approved by the student advisory committee

Chairman (Dr. K. RADHIKA) _____________________
Assistant Professor
Department of Genetics and Plant Breeding
College of Agriculture
ANGRAU
Rajendranagar, Hyderabad

Co-
Chairman (Dr. G. PADMAVATHI) _____________________
Senior Scientist
Department of Plant Breeding
Directorate of Rice Research
Rajendranagar, Hyderabad.

Member (Dr. V. PADMA) ______________________
Associate Professor
Department of Plant Physiology
College of Agriculture
ANGRAU
Rajendranagar, Hyderabad.
CONTENTS

CHAPTER TITLE PAGE NO.
I INTRODUCTION
II REVIEW OF LITERATURE
III MATERIAL AND METHODS
IV RESULTS
V DISCUSSION
VI SUMMARY
LITERATURE CITED
APPENDICES
LIST OF TABLES

TABLE PAGE
TITLE
NO. NO.
1 QTL(s) gene identified for salt-tolerance
2 Details of parents used for parental polymorphism
3 Standard Evalution Score (SES) of visual alkalinity injury
4 Chemicals required for the preparation of extraction buffer
5 Details for Preparation of Master Mix for PCR
6 Details of thermal profile for DNA amplification using PCR
7 Details of SSR markers used for mapping of F population
2
of CSR 27 × Samba mahsuri
8 Phenotypic performance of parents and F population of
2:3
the cross CSR 27 × Samba mahsuri for alkalinity tolerance
9 Correlation Co-efficient among phenotypic traits studied in
193 F families
2:3
10 Putative QTLs for alkalinity tolerance at reproductive stage
by interval mapping in the F population derived from
2:3
CSR 27 and Samba mahsuri
LIST OF ILLUSTRATIONS

FIGURE PAGE
TITLE
NO. NO.
1 Frequency distribution of different phenotypic traits
2 QTL maps for different phenotypic traits
LIST OF PLATES
PLATE PAGE
TITLE
NO. NO.
1 Gel showing some of the markers used for parental
polymorphism survey of Samba mahsuri and CSR 27
2 Segregating pattern of RM 26086 in F population of
2
CSR 27 x Samba mahsuri
LIST OF APPENDICES

PAGE
APPENDIX TITLE
NO.
Ι Classification of saline and sodic soils according to USDA
Soil Salinity Laboratory (USSL, 2005)
ΙΙ List of Chemicals
ΙΙΙ Buffers and stock solutions
ΙV List of Equipments
ACKNOWLEDGEMENTS
I thank the almighty GOD for his love and blessings without which I would
not have been able to complete my studies hitherto and present this piece of work.
I would like to take this opportunity to place my profound debt of
gratitude on record to my major advisor and Chairman of the Advisory Committee,
Dr. K. Radhika Assistant Professor, Department of Genetics and Plant Breeding,
College of Agriculture, Rajendranagar, Hyderabad for her valuable suggestions,
encouragement, affection, personal guidance, keen interest, immeasurable help and
inspiration given to me throughout my work and who has made it possible to bring
out this thesis.
I am equally and deeply indebted to my Co-Chairman of Advisory Committee
Dr. G. Padmavathi, Senior Scientist, Department of Plant Breeding, Directorate
of Rice Research, Rajendranagar, Hyderabad for her encouragement, technical
suggestions and constructive criticism in perfect conduct of the experimental work
and presentation of thesis.
I owe my effusive thanks to the member of my Advisory Committee
Dr. V. Padma, Associate Professor, Department of Plant Physiology, College of
Agriculture, Rajendranagar, Hyderabad for her timely help, co-operation, constant
guidance and valuable suggestions throughout the period of investigation.
I render my special thanks to Dr. M. Ganesh, Professor and Head,
Department of Genetics and Plant Breeding, College of Agriculture, Rajendranagar,
Hyderabad for his kind, enthusiastic and spontaneous co-operation during my
research work.
I render my special thanks to Dr. K. Subramanyam Reddy, Professor,
Department of Statistics and mathematics, College of Agriculture, Rajendranagar,
Hyderabad for his kind, enthusiastic and spontaneous co-operation during my
statistical analysis.
My sincere thanks to Dr. M. V. Brahmeswar Rao and Dr. K. Murali
Krishna for their support and encouragement during the course of my study. I am
very much thankful to all of my department staff for their encouragement during the
course of my study.
It is time to surface out genuflect love and affectionate gratitude to my
dearest parents, Smt. Laxmi and Sri. Narsaiah for their blessings, inspiration,
encouragement and moral support throughout my educational career.
I owe much to my loving brothers Narender-Sruthi, Surendra-Jyothi and
Naresh Gandhi and Prince Adithya, sister Aruna for their everlasting affection,
co-operation and encouragement in my life. They have been my motivation and best
critics.
Words fail to express my heartfelt thanks to my special friend,
K. Bhanupriya for her co-operation, moral support and transcendent suggestions
during the course of my study.
Words are not enough to express my heartfelt thanks to my best friends
Krishna veni, Pramila, Ravichandra, Rebelson, Karunakar Reddy, Raju and
my beloved juniors Swapna, Srilatha, Sujatha and Aruna for their unparallel
affection, encouragement and moral support, which instilled in me confidence to
proceed forward.
I am very much thankful to Janaki Ramayya, K. Ramesh and Ramdeen
for their timely support, spending their valuable time for the completion of my
research work successfully.
My special thanks to my classmates, Usha Rani, Raghu bayya, Minnie,
Chandresh Singh, Maruti, Priyatam Kumar, Shreya, Anjali, Shirisha,
Shailaja, Ravi and Vijay for their splendid company and cooperation all through
the period of my study.
Finally I am very much thankful to Acharya N. G. Ranga Agricultural
University and Government of Andhra Pradesh for the financial support
received during the course of investigation.

Date: (TALARI JYOTHSNA)