Molecular Characterization of VIM Carbapenemases in the Arabian Peninsula

137 Pages·2016·15.99 MB·English

by  Nour Yahfoufi

United Arab Emirates University
Scholarworks@UAEU
Theses Electronic Theses and Dissertations
2-2014
Molecular Characterization of VIM
Carbapenemases in the Arabian Peninsula
Nour Yahfoufi
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Recommended Citation
Yahfoufi, Nour, "Molecular Characterization of VIM Carbapenemases in the Arabian Peninsula" (2014).Theses. 262.
https://scholarworks.uaeu.ac.ae/all_theses/262
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UniteAdr abE miratUensi versity
Colleogf M edicianned H ealtShc iences
DepartmeonfMt i.c orbioloagnyd I mmunology
MOLEUCL R CHARACTERlAZn ON OFV IMC ARBAPNEEMASEISN
THEA RABAINP E INSULA
NoruY ahofuif
ThiTsh eissi ss ubimtteidnp atrifaullfi lenmotft hree quirefmorte hnet stM eaorsf
Medciinaen Hde alctihe nicnMe isco rbiloogayn Id mmnuoglyod egree
Undetrh ee dcitiroofPn r eosfsToirob rP al
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DeclaratoifoO nr iginWaolr k Page
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Unie ri t(yU AE) a ndt haeu thooftr h teh edsiis se/rtattiidto "lnMe ocluerl a
haarcteriozfaV t]iMoC na rbeanpemeas inA ratbhieaP nen nisulhaee"rb y
olemndlelycae r thatth itsh ie/ id erttiaoinsa no rigiwnoarlkd onaen d
prepabr meedu ndtehrge u idaonfc eP Troibf.oP ra iln Ctohllege eo fM edciien
andH ealht ceinceDse,p tamrenoftM icorbigoyla ondI mmunoloAgtUy A EU.
ThiW orkh a notp reivousflonyn etdh bea sfiorst he awoafar ndyd egree,
diopmlao ri mliatrie t altt hiosra nyo thuenrei rviyst.T heM atreiablosr rowed
form otheoruc resan di cnlduedi nm y thedsiissset/ratihoanv e bpeorepne rly
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a knolwdeged.
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tudenitg'tnsuae r. ..... ). . Date ./)�..lcJ. of L. I
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Copyr©i g2h01t4b y ouYra hofuif
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SignatPuargee s
ApproveBdy dvio ryC ommittee:
1)A dvoir ' TiborP alM D, PhD
Title' Professor
DepartmeMnitrc:o bioalnoIdgm ymn uoolgy
Int itutioCno:l gleoe fM edicainnHdee a lh tcienUcAeEsU,
Dat2e8-0 12-014
2)M emebr: Bassma AliP hD
Title: Professor
DepartmePnatt:h ology
Int itutioCno:lg leoe fM edicainnHdee a lhS tcienUcAEeUs ,
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3) Memb:e r AgnesS onnevenMDd, PhD
Tilt:e AssisPtraonfte ssor
DepartmeMnitc:r obiaonlIdom gmyuo ngoyl
IsntitutiCoonll:ge eo fM edicainnHdee ahlS tcienUcAEeUs ,
Signature
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Thei Examinatioonm mittee:
1)A dyoir . TiboPra lM D PhD
TIlte: Profoer
DepartmeMnItc:r obiaonIldom ugmnyoo gly
InstitutCloOllnge:eo f MedaincHdie anlheS t cienUcAeEsU,
Dat2e80 -12-014
2)M ember: BassamA liP hD
Title: Profoers
DepartmePnatt:h ology
InstitutCioollnge:eo fM edicainnHdee ahlS tcienUcAeEsU,.
Signature Dat2e8- 012-014
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3)M ember: gnesS onnevenMdD ,P hD
Title: AssisPtraonfte ssor
DepartmeMnitc:r olgboyia onIdm muongoyl
IsntitutioCnol:lge eo fM edicainnHdee alStchi enUcAeEsU,
Signature Dat2e80 -12-014
cont.
4)E xteErnxamlai ner:D avidL esliPea tersMoDn, PhD
Title: Professor
Department: CentfeoCrril n icRaels earch
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ABT RACT
The emegrence agonlbdla spreaodf carbaperneesmi stant
Entearcotberiaic aenaa el naningw orld-pwhiedneo metnhoaantlo s affecttsh e
MiddlEea t duet ol miitetdr eatomepntti oforn sucihne fctiaonndst heir
commoan o ciawtiitohhn gi hl eevlof f atlatiy. mTohset p oitrmanmtec hiasnomf
ucrhse itsanicsce a eudsb yt hpeor ductoifvo anr icoaubr apeneemnayzsmee sI.n
thAera biPaenni nls,asu of ar,N DMa nOd XA4-8ilekc abrapenemhaasvbeees e n
reporwtheiedl e, les\h eer otheenrz ymee.sg,V. I MI, MP andK PC area los
commonflou ndO.u ra imw ast os yesmtatliylc laookf or tVheIM -type
carbpaenemaasmeosn glc oali slotaesa ndt o chaacrtertihzeeig ren iect
bacrkogund.
Inailyt,lis crneienigs otlefasor m AbuD habhio sapliswt ei denetdai sfiingle
Enterobcalcotaecrse tairen ac arryintgh VeI M-4 aleell.T hiwsa st hfei rssutc h
isaotleev erre porftrmeo tdh Peen insSuublsaeu.qe n,ti lvnyestiingigas totlefasor m
Kuwa,Si atuAdraib ,iO amana ntdh UeA E,w ei deinefitda f urth1e1irs otleoasn,e
E.c loacfreoameS auAdria bitaw,of o rmO mano,n efor mK uwaaintda los one
E chericchoilfaoir mt hciosu rnytB.e sitdheess el,ta ttcehoreu r nyat losp rovided
sxiK lebsipenleluami oanie sotleAasl.s lt irnapsr oudcetdh VeJ M-v4ar iaonftt h e
enzymea sd eteirnmebdyt hsee quenocfti ehniggr en esI.na lcla setsh,e gweanse
lcoaetd opanl smiodfsv ayrinsgie zse,ti henro ny-ptabolreb elongtoit nhge
lnAciC gorupm;os to f twheeemrc ojnguaiteva ntdh ecyo mmonhlaryb oroetdh er
13-laamcatsgeen ess,u cahsC TX-oMr C-M4YI. na lslta rintshVe IM -4g enwea s
locawtitehdia c nal sIsi tnegr-owni tsho mvear iatbieotnwset ehgnee ncea steets
prese-nsti lmaitro s tirnapsr eovuisliyd eiinfetdi nN ortAhfr icaan dIa tyl,
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sugget ign thei bpiiotol yfs pera.dC lnoatlpy inrgee altehdat hte l airetvelhyi gh
icnidenocfe V-IpMruocdeErn treobcateriea ecnecaoeurneitdnK uv.iawta sn ot
duet ot hsep erad opfat riaac ru olcnleb,u mto sptr oabblwya st hrese uolftt he
trafnero fa nI cn C pImai dc,o -hoarribbnlga vaIMn4db l acM.iY tn-4oK lebsiella
pneumoena inaEd. c oli.
Ourd atsah otwh ,ab teyoNnDdM a ndO XA-4i8ek-,Vl IMt ypceab rapenemases
arteh teh dim rosctom mon oliaestsi nt hAer aibnaP enisnulat.h eiFvrnue risgtation
i needteomd o niotrt hsep erad colnfoe asn gde neisn tghieo nr.e
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ACKNOWLED GME TS
I takteh iosp ptouryn ittoe xpremsys p rooufngdr iattduet o msyu pveIorsr
PreosfsToirob rP al,a sw ellas D r.g neso nnveenfdor t ehigru idaanncde
monitnogr.i
Fosru er,Iw oulhda e b eeunn ablaec ihteovte h is wtitahsotkuh te siuerpvr ision
as waesl lth heelo pft hger oumpe mebrasn ds tfai fnb acrtieoglyl oaboratories,
M A. kelaG hzaawMir,. M ohammedA I-Hjaa ndM rs.D aniDaa wricSho.m, y
graicoutsh antko eer oyn oef t hem.
Thantkosfo or t he cotnartirnuosesld t,oP r.oV finceRnott imDir,W. a afJ amal,
Proft.e fh ibl,S eDiArfIA. -b rDir,A. m niaA IJ-aardnDir,S. a fiGniraigz,s D r.
Rah aHn ashmyea ndD rW.a heedi qT.a r
Iw oulda los liet koe xtemnyds icneer greientgtsoP ro.B fassamA li aanldl t o
fauctlym emebrisnt hDee ptamreonftM icorbiloogayn Id mmunloogCyo,ll geeo f
Mediicena nHde alStcehinc esU,ni tAedra b Eamtiers eUsrnyiit.v
Ina ddiiotnd eae tph aynoktu o my baronatdhl melyr sif ernds.
NouYra hofuif
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