Models of Anaerobic Infection: Proceedings of the third Anaerobe Discussion Group Symposium held at Churchill College, University of Cambridge, July 30–31, 1983, followed by the abstracts of the first meeting of the Society for Intestinal Microbial Ecolog

286 Pages·1984·7.21 MB·English

by  M. J. Kelly (auth.)

MODELS OF ANAEROBIC INFECTION
NEW PERSPECTIVES IN CLINICAL MICROBIOLOGY
Brumfitt W, ed Hamilton-Miller JMT, assist. ed: New perspectives in clinical micro
biology. 1978. ISBN 90-247-2074-5
Tyrrell DAJ, ed: Aspects of slow and persistent virus infections. 1979.
ISBN 90-247-2281-0
Brumfitt W, Curcio L, Silvestri L, eds: Combined antimicrobial therapy. 1979.
ISBN 90-247-2280-2
van Furth R, ed: Developments in antibiotic treatment of respiratory infections. 1981.
ISBN 90-247-2493-7
van Furth R, ed: Evaluation and management of hospital infections. 1982.
ISBN 90-247-2754-5
Kuwahara S, Pierce NF, eds: Advances in research on cholera and related diarrheas 1.
1983. ISBN 0-89838-592-X
Ristic M, Ambroise-Thomas P, Kreier J, eds: Malaria and babesiosis: Research findings
and control measures. 1984. ISBN 0-89838-675-6
Kuwahara S, Pierce NF, eds: Advances in research on cholera and related diarrheas 2.
1984. ISBN 0-89838-680-2
Takeda Y: Bacterial diarrheal diseases. 1984. ISBN 0-89838-681-0
Hill MJ, Borriello SP, Hardie JM, Hudson MJ, Lysons RJ, Tabaqchali S, eds: Models
of anaerobic infection.
1984. ISBN 0-89838-688-8
MODELS OF ANAEROBIC
INFECTION
Proceedings of the third Anaerobe Discussion Group Symposium held at
Churchill College, University of Cambridge, July 30-31, 1983, followed by
the abstracts of the first meeting of the Society for Intestinal Microbial
Ecology and Disease, Boston, November 1983
edited by the steering committee of the Anaerobe Discussion Group
editor in chief:
M.J. HILL
members:
S.P. BORRIELLO, J.M. HARDIE, M.J. HUDSON, R.J. LYSONS,
S. TABAQCHALI
1984 MARTINUS NIJHOFF PUBLISHERS 1rI...
1111
a member of the KLUWER ACADEMIC PUBLISHERS GROUP
DORDRECHT / BOSTON / LANCASTER •
Distributors
for the United States and Canada: Kluwer Academic Publishers, 190 Old Derby
Street, Hingham, MA 02043, USA
for the UK and Ireland: Kluwer Academic Publishers, MTP Press Limited,
Falcon House, Queen Square, Lancaster LA1 1RN, England
for all other countries: Kluwer Academic Publishers Group, Distribution Center,
P.O. Box 322, 3300 AH Dordrecht, The Netherlands
Library of Congress Cataloging in Publication Data
ISBN-13: 978-94-009-6056-5 e-ISBN-13: 978-94-009-6054-1
DOl: 10.1007/978-94-009-6054-1
Copyright
© 1984 by Martinus Nijhoff Publishers, Dordrecht.
Softcover reprint of the hardcover I st edition 1984
All rights reserved. No part of this publication may be reproduced, stored in a
retrieval system, or transmitted in any form or by any means, mechanical,
photocopying, recording, or otherwise, without the prior written permission of
the publishers,
Martinus Nijhoff Publishers, P.O. Box 163, 3300 AD Dordrecht,
The Netherlands.
v
PREFACE
The ADG held its first International Symposium at Churchill College,
Cambridge, in July 1979. The second symposium was also held at Churchill
College on 30-31 July, 1981, and this, the third, took place at the same
college on 30-31 July,. 1983.
The meeting was structured in a format which we hoped would appeal to
the full range of our membership. The philosophy of the ADG is that
medical microbiologists, veterinarians, toxicologists and dental
bacteriologists have much to learn from each other and can best be
achieved by bringing these various disciplines together frequently and
in informal surroundings. Again the symposium was very generously
sponsored by May and Baker Limited who met all costs of the meeting and
entertained us splendidly. David Jackson and Donald Bedford were re
sponsible for coordinating with the ADG on behalf of May and Baker and,
as usual, gave us their full cooperation.
This book contains the full-length papers, followed by the posters
presented at the meeting.
This book also serves as a vehicle for the abstracts of the first meeting
of the Society for Intestinal Microbial Ecology and Disease, SIMED, held
in Boston, Massachusetts. An introduction to this new society by its
President, Sydney M. Finegold, M.D., precedes the abstracts.
M.J. Hill
VI
CONTENTS
Preface v
List of contributors VIII
Introduction to the Anaerobe Discussion Group
M.J. Hill XI
A guinea-pig model demonstrating synergy between Escherichia coli
and Bacteroides fragilis in infected surgical wounds.
M.J. Kelly
An animal model of peritonitis and the effect of anti-microbial
prophylaxis and therapy.
C.E. Nord and G. Lahnborg 9
The pathology of animal models of antibiotic-associated colitis.
A. Price 23
Bacterioides fragilis, Propionibacterium acnes and Peptostreptococcus
anaerobius in experimental otitis media in guinea-pig.
M. Thore, L.G. Burman and S.E. Holm 39
Success and failure in the association of anaerobes from human sub
gingival plaque with conventional and gnotobiotic rats.
B. Guggenheim, R. Gmur and C. Wyss 51
Enterocolitis in pigs.
R.J. Lysons 67
Animal models of Clostridium spiroforme-mediated diarrhoea (iota entero
toxaemia) of rabbits.
R.J. Carman and S.P. Borriello 77
Ovine footrot: Histopathology of a synergic disease.
Paul M. Hine 85
A model for investigating polymorph function ln the presence of non
sporing anaerobes.
H.R. Ingham and Penelope R. Sisson 99
Effect of Bacteroides fragilis lipopolysaccharide and capsular poly
saccharide on polymorph function and serum killing.
Janet C. Connolly and Soad Tabaqchali 109
The influence of Bacteroides sp. on the opsonic activity of human serum
and its effect on the phagocytosis of various bacteria.
C.G. Gemmell, G.R. Jones and R. McNaught 119
In vitro and in vivo studies on the pertubation or host derence by
black-pigmented Bacteroides species.
G. Sundqvist and J. Carlsson 129
In vivo metabolism in the rumen fermentation.
R. E. Hungate 139
VII
Mathematical models.
P.N. Hobson 151
The application of continuous culture techniques to the study of oral
anaerobes.
Ailsa S. McKee, Ann S. McDermid, P.D. Marsh and D.C. Ellwood 165
In vitro models of the mammalian caecum and colon.
I.R. Rowland 177
Tissue culture systems for the examination of bacterial virulence.
L.G. Giuglano, M. Barer, G.F. Mann and B.S. Drasar 189
Adherence in anaerobes: An in vitro model of adhesion in Clostridium
difficile.
M.R. Barer
201
Adhesion in Clostridia.
P.D. Walker
211
Posters presented at the Third Anaerobe Discussion Group Symposium,
1983
221
Introduction to the Society for Intestinal Microbial Ecology and Disease
by S.M. Finegold 261
Abstracts of the first meeting of the Society for Intestinal Microbial
Ecology and Disease, Boston, Massachusetts, 1983 263
VIII
LIST OF CONTRIBUTORS
Barer, M.R. Department of Medical Microbiology, London
School of Hygiene and Tropical Medicine,
Keppel Street (Gower St.), London, WC1E 7HT.
Borriello, S.P. Division of Communicable Diseases, Clinical
Research Centre, Watford Road, Harrow,
Middlesex, HA1 3UJ.
Burman, L.G. Lasarattet, Klt Bakt Lab., 721 89 Veastaras,
Sweden.
Carman, R.J. Animal Unit, Charing Cross Hospital Medical
School, St. Dunstan's Road, London, W6 8RP.
Car lsson , J. Department of Endodontics, School of
Dentistry, University of Ume!, Ume: 6, Sweden.
Connolly, Janet C. Department of Medical Microbiology, St.
Bartholomew's Hospital Medical College,
London, EC1.
Drasar, B.S. Department of Medical Microbiology, London
School of Hygiene and Tropical Medicine,
Keppel Street (Gower St.), London, WC1 7HT.
Ellwood, D.C. PHLS Centre for Applied Microbiology and
Research, Pathogenic Microbes Research
Laboratory, Porton Down, Salisbury, Wiltshire,
SP4 OJG.
GelllDel, C.G. University of Glasgow, Bacteriology
Department, Royal Infirmary, Glasgow, G4 OSF.
Giugl1ano, L.G. Department of Medical Microbiology, London
School of Hygiene and Tropical Medicine,
Keppel Street (Gower St.), London, WC1E 7HT.
Guggenheim, B., University of Zurich, Zahnarzliches Institut,
Gmur, R. and Plattenstrasse 11, CH-8028, Zurich.
Wyss, C.
Hill, M.J. PHLS Centre for Applied Microbiology and
Research, Bacterial Metabolism Research
Laboratory, Porto n Down, Salisbury, Wiltshire,
SP4 OJG.
Hine, Paul M. Department of Bacteriology R&D, The Wellcome
Research Laboratories, Langley Court,
Beckenham, Kent, BR3 3BS.
Hobson, P.N. The Rowett Research Institute, Microbial
Biochemistry Department, Greenburn Road,
Bucksburn, Aberdeen, AB2 9SB.
Holm, S.E. Lasarattet, KLT Bakt Lab., 721 89 Veastaras,
Sweden.
IX
Hungate, R.E. University of California, Department of
Bacteriology, Davis California 95616, USA.
Ingham, H.R. Department of Microbiology, Institute of
Pathology, General Hospital, Westgate Road,
Newcastle-Upon-Tyne, NE4 6BE.
Jones, G.R. University of Glasgow, Bacteriology
Department, Royal Infirmary, Glasgow, G4 OSF.
Kelly, M.J. Department of Surgery, Southmead General
Hospital, Westbury-on-Trym, Bristol, BS10 5NB.
Lysons, R.J. ARC Institute for Research on Animal Diseases,
Compton, Nr. Newbury, Berkshire, RG16 ONN.
McDermid, Ann S. PHLS Centre for Applied Microbiology and
Research, Pathogenic Microbes Research
Laboratory, Porton Down, Salisbury, Wiltshire,
SP4 OJG.
McKee, Ailsa S. PHLS Centre for Applied Microbiology and
Research, Pathogenic Microbes Research
Laboratory, Porton Down, Salisbury, Wiltshire,
SP4 OJG.
McNaught, R. University of Glasgow, Bacteriology
Department, Royal Infirmary, Glasgow, G4 OSF.
Mann, G.F. Department of Medical Microbiology, London
School of Hygiene and Tropical Medicine,
Keppel Street (Gower St.), London, WC1E 7HT.
Marsh, P.D. PHLS Centre for Applied Microbiology and
Research, Pathogenic Microbes Research
Laboratory, Porton Down, Salisbury, Wiltshire,
SP4 OJG.
Nord, C.E. and The National Bacteriological Laboratory,
Lahnborg, G. S-105 21 Stockholm, Sweden.
Price, A. Department of Histopathology, Northwick Park
Hospital and Clinical Research Centre, Watford
Road, Harrow, Middlesex, HA1 3UJ.
Rowland, I.R. British Industrial Biological Research
Association, Woodmansterne Road, Carshalton,
Surrey, SM5 4DS.
Sisson, Penelope R. Department of Microbiology, Institute of
Pathology, General Hospital, Westgate Road,
Newcastle-Upon-Tyne, NE4 6BE.
Sundqvist, G. Department of Endodontics, School of
Dentistry, University of Ume~, Ume! 6, Sweden.
Tabaqchali, Soad Department of Medical Microbiology, St.
Bartholomew's Hospital Medical College,
London, EC 1 •
x
Thore, M. Lasarattet, KLT Bakt Lab., 721 89 Veastaras,
Sweden.
Walker, P.D. Department of Bacteriology R&D, The Wellcome
Research Laboratories, Langley Court,
Beckenham, Kent, BR3 3BS.
Wyss, C. University of Zurich, Zahnarzliches Institut,
Plattenstrasse 11, CH-8028 ZUrich.